Farklı tipteki aljinat dental ölçü maddelerinin sitotoksisite yönünden değerlendirilmesi
Abstract
Purpose: The aim of this study was to assess the cytotoxicity of different types of alginate impression materials.
Material and Method: Cavex CA37, A3KROM, ALGINPLUS FAST ve ORALGHINE alginate impression materials were used. According to the manufacturer’s instructions, alginates were mixed with serum physiological to obtain alginate specimens. Specimens were
performed in sterile with ethylene oxide gas and then placed on to the L929 fibroblast cell culture. According to the 1999 ISO 10993-5 protochols, cytotoxicity were determined by means of agar overley test.
Results: According to the lisis of the cells, ORALGH<NE and Cavex CA37; 4-4-4-5-5, ALGINPLUS and A3KROM; 2-3-3-3-2 were determined. Values of the cytotoxicity were determined 4,4 for ORALGH<NE and Cavex CA37, whereas 2,6 for ALGINPLUS and A3KROM.
Conclusion: Cytotoxicity degree of the alginates were different because of the composition of the alginates. It was determined that ORALGHINE and Cavex CA37 were severely cytotoxic, ALGINPLUS and A3KROM were moderately cytotoxic.
ÖZET
Amaç: Bu in vitro çalışmanın amacı, farklı tipteki aljinat ölçü maddelerini sitotoksisite yönünden değerlendirmektir.
Gereç ve Yöntem: Farklı tipteki dört aljinat, Cavex CA37, A3KROM, ALGINPLUS FAST ve ORALGHINE, üretici firmaların talimatlar doğrultusunda belirtilen oranlarda, serum fizyolojik ile karıştırılarak aljinat numuneleri hazırlandı. Hazırlanan numuneler etilen oksit gazıyla steril edildi ve L929 fibroblast hücre serisi kullanılarak elde edilen kültüre yerleştirildi. Agar overley testi kullanılarak sitotoksisitenin belirlenmesinde ISO 1999 yıl 10993-5 numaral protokolü takip edildi.
Bulgular: Hücrelerin lizis miktarına göre yapılan puanlama değerleri ORALGHINE ve Cavex CA37 için; 4-4-4-5-5, ALGINPLUS ve A3KROM için; 2-3-3-3-2 olarak tespit edildi. Sitotoksisite değerleri de ORALGHINE ve Cavex CA37 için 4.4, ALGINPLUS ve A3KROM için ise 2.6 olarak bulundu.
Sonuçlar: İçerik farklılığına bağlı olarak aljinatlarn sitotoksisite değerleri farklılık göstermiştir. ORALGHINE ve Cavex CA37’nin belirgin derecede sitotoksik olduğu, ALGINPLUS ve A3KROM’ un ise makul derecede sitotoksik olduğu belirlendi.
Anahtar kelimeler: Sitotoksisite, aljinat.Keywords
References
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- American Dental Association Specification No 18-1992. Alginate Impression Material.
- International Standard ISO 10993-5. 1999. Biological evaluation of medical devices- Part 5: Tests for in vitro cytotoxicity. Geneva: International Organization for Standardization.
- Liu CM, Huang FM, Yang LC, Chou LSS, Chou MY. Cyctotoxic effects of gingival retraction cords on human gingival fibroblast in vitro. J Oral Rehabil 2004;31:368-372.
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
November 1, 2011
Submission Date
November 1, 2011
Acceptance Date
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Published in Issue
Year 2009 Volume: 12 Number: 1