Öz
Purpose: The aim of this study was to assess the cytotoxicity of different types of alginate impression materials.
Material and Method: Cavex CA37, A3KROM, ALGINPLUS FAST ve ORALGHINE alginate impression materials were used. According to the manufacturer’s instructions, alginates were mixed with serum physiological to obtain alginate specimens. Specimens were
performed in sterile with ethylene oxide gas and then placed on to the L929 fibroblast cell culture. According to the 1999 ISO 10993-5 protochols, cytotoxicity were determined by means of agar overley test.
Results: According to the lisis of the cells, ORALGH<NE and Cavex CA37; 4-4-4-5-5, ALGINPLUS and A3KROM; 2-3-3-3-2 were determined. Values of the cytotoxicity were determined 4,4 for ORALGH<NE and Cavex CA37, whereas 2,6 for ALGINPLUS and A3KROM.
Conclusion: Cytotoxicity degree of the alginates were different because of the composition of the alginates. It was determined that ORALGHINE and Cavex CA37 were severely cytotoxic, ALGINPLUS and A3KROM were moderately cytotoxic.
ÖZET
Amaç: Bu in vitro çalışmanın amacı, farklı tipteki aljinat ölçü maddelerini sitotoksisite yönünden değerlendirmektir.
Gereç ve Yöntem: Farklı tipteki dört aljinat, Cavex CA37, A3KROM, ALGINPLUS FAST ve ORALGHINE, üretici firmaların talimatlar doğrultusunda belirtilen oranlarda, serum fizyolojik ile karıştırılarak aljinat numuneleri hazırlandı. Hazırlanan numuneler etilen oksit gazıyla steril edildi ve L929 fibroblast hücre serisi kullanılarak elde edilen kültüre yerleştirildi. Agar overley testi kullanılarak sitotoksisitenin belirlenmesinde ISO 1999 yıl 10993-5 numaral protokolü takip edildi.
Bulgular: Hücrelerin lizis miktarına göre yapılan puanlama değerleri ORALGHINE ve Cavex CA37 için; 4-4-4-5-5, ALGINPLUS ve A3KROM için; 2-3-3-3-2 olarak tespit edildi. Sitotoksisite değerleri de ORALGHINE ve Cavex CA37 için 4.4, ALGINPLUS ve A3KROM için ise 2.6 olarak bulundu.
Sonuçlar: İçerik farklılığına bağlı olarak aljinatlarn sitotoksisite değerleri farklılık göstermiştir. ORALGHINE ve Cavex CA37’nin belirgin derecede sitotoksik olduğu, ALGINPLUS ve A3KROM’ un ise makul derecede sitotoksik olduğu belirlendi.
Anahtar kelimeler: Sitotoksisite, aljinat.Anahtar Kelimeler
Kaynakça
- Lloyd CH, Scrimgeour SN. ‘dental materials: 1994 Literature rewiew,’ J Dent 1996;24:153-184.
- Roberta T, Federico M, Federica B, Antonietta CM, Sergio B, Ugo C. Study of the potential cyctotoxicity of dental impression materials. Toxicology in Vitro 2003;17:657-662.
- Polyzois GL, Dahl JE, Hensten-Pettersen A.’Biological Testing of dental materials: Development of national and international standarts,’ J Biomater Appl 1995;9:355-361.
- McCabe JF. Di hekimliinde Maddeler Bilgisi, 1999, s.110-112.
- Sivers JE, Johnson GK. Adverse soft tissue response to impression procedures: report of case. J Am Dent Assoc. 1988;116(1):58-60.
- American Dental Association Specification No 18-1992. Alginate Impression Material.
- International Standard ISO 10993-5. 1999. Biological evaluation of medical devices- Part 5: Tests for in vitro cytotoxicity. Geneva: International Organization for Standardization.
- Liu CM, Huang FM, Yang LC, Chou LSS, Chou MY. Cyctotoxic effects of gingival retraction cords on human gingival fibroblast in vitro. J Oral Rehabil 2004;31:368-372.
- Wataha JC, Hanks CT, Craig RG. The effect of cell monolayer density on the cytotoxicity of metal ions which are released from dental alloys. Dent Mater 1993;9(3):172-176.
- Bumgardner JD, Lucas LC. Cellular response to metallic ions released from nickel-chromium dental alloys. J Dent Res 1995;74(8):1521-1527
- Schedle A, Samorapoompichit P, Rausch-Fan XH, Franz A, Füreder W, Sperr WR, Sperr W, Ellinger A, Slavicek R, BoltzNitulescu G, Valent P. Response of L-929 fibroblasts, human gingival fibroblasts, and human tissue mast cells to various metal cations. J Dent Res 1995;74(8):1513-1520.
Öz
Amaç: Bu in vitro çal man n amac , farkl tipteki aljinat ölçü maddelerini sitotoksisite yönünden de erlendirmektir. Gereç ve Yöntem: Farkl tipteki dört aljinat, Cavex CA37, A3KROM, ALGINPLUS FAST ve ORALGHINE, üretici firmalar n talimatlardo rultusunda belirtilen oranlarda, serum fizyolojik ile kar t r larak aljinat numuneleri haz rland . Haz rlanan numuneler etilen oksit gaz yla steril edildi ve L929 fibroblast hücre serisi kullan larak elde edilen kültüre yerle tirildi. Agar overley testi kullan larak sitotoksisitenin belirlenmesinde ISO 1999 y l 10993-5 numaral protokolü takip edildi. Bulgular: Hücrelerin lizis miktar na göre yap lan puanlama de erleri ORALGH
Anahtar Kelimeler
Kaynakça
- Lloyd CH, Scrimgeour SN. ‘dental materials: 1994 Literature rewiew,’ J Dent 1996;24:153-184.
- Roberta T, Federico M, Federica B, Antonietta CM, Sergio B, Ugo C. Study of the potential cyctotoxicity of dental impression materials. Toxicology in Vitro 2003;17:657-662.
- Polyzois GL, Dahl JE, Hensten-Pettersen A.’Biological Testing of dental materials: Development of national and international standarts,’ J Biomater Appl 1995;9:355-361.
- McCabe JF. Di hekimliinde Maddeler Bilgisi, 1999, s.110-112.
- Sivers JE, Johnson GK. Adverse soft tissue response to impression procedures: report of case. J Am Dent Assoc. 1988;116(1):58-60.
- American Dental Association Specification No 18-1992. Alginate Impression Material.
- International Standard ISO 10993-5. 1999. Biological evaluation of medical devices- Part 5: Tests for in vitro cytotoxicity. Geneva: International Organization for Standardization.
- Liu CM, Huang FM, Yang LC, Chou LSS, Chou MY. Cyctotoxic effects of gingival retraction cords on human gingival fibroblast in vitro. J Oral Rehabil 2004;31:368-372.
- Wataha JC, Hanks CT, Craig RG. The effect of cell monolayer density on the cytotoxicity of metal ions which are released from dental alloys. Dent Mater 1993;9(3):172-176.
- Bumgardner JD, Lucas LC. Cellular response to metallic ions released from nickel-chromium dental alloys. J Dent Res 1995;74(8):1521-1527
- Schedle A, Samorapoompichit P, Rausch-Fan XH, Franz A, Füreder W, Sperr WR, Sperr W, Ellinger A, Slavicek R, BoltzNitulescu G, Valent P. Response of L-929 fibroblasts, human gingival fibroblasts, and human tissue mast cells to various metal cations. J Dent Res 1995;74(8):1513-1520.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Original Research Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Kasım 2011 |
| Gönderilme Tarihi | 1 Kasım 2011 |
| Yayımlandığı Sayı | Yıl 2009Cilt: 12 Sayı: 1 |
Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.
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