Abstract
Objectives: Describe rat gingiva histology with a traditional hematoxylin-eosin stain and non-traditional Goldner trichrome stain technique.
Materials and Methods: Samples of gingiva of adult Wistar rats were processed for histological purposes. The classic hematoxylin and eosin stain and Goldner trichrome stain technique were applied to the tissue. Digital microphotography of the three parts of the gingiva were obtained.
Results: For the free gingiva its oral, sulcular and junctional epitheliums were described. For the attached gingiva, its oral epithelium and for the interdental gingiva, its particular epithelium was detailed as well. Each layer of the stratum of the five epitheliums were specified and the cellular morphology explained. The gingiva lamina propria was also detailed. Among others, structures such as the gingival groove, the collagen fibers corresponding with the transseptal fibers, the bone on the alveolar crest, the enamel space, the dentin and the pulp were also identified.
Conclusions: Goldner’s trichrome stain allows simpler tissue identification, easier epithelium stratum establishment and detailed cellular morphology observation for the rat free, attached and interdental gingiva.
Ethical Statement
The experiment received approval from the Biomedical Experimentation Animal Ethics Committee under the internal code 013-017
Thanks
The author would like to acknowledge the Laboratory of Histology of the Universidad del Valle, Cali, Colombia.
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Details
| Primary Language | English |
|---|---|
| Subjects | Periodontics |
| Journal Section | Original Research Articles |
| Authors | |
| Publication Date | June 30, 2025 |
| Submission Date | January 22, 2025 |
| Acceptance Date | April 5, 2025 |
| Published in Issue | Year 2025Volume: 28 Issue: 2 |
Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.
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