Abstract
References
- 1. Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J. Biomed. Mater. Res. 2008;86:523-529
- 2. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeek B, De Munck J. Bonding effectiveness of a new ‘multi-mode’adhesive to enamel and dentine. J. Dent. 2012;40:475-484
- 3. Perdigão J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multipurpose dentin adhesive. Am. J. Dent. 2012;25:153
- 4. Perdigão J, Muñoz MA, Sezinando A, Luque-Martinez IV, Staichak R, Reis A, Loguercio AD. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper. Dent. 2014;39:489-499
- 5. Caughman WF, Rueggeberg F. Shedding new light on composite polymerization. Oper. Dent. 2002;27:636-638
- 6. Mills RW, Uhl A, Blackwell GB, Jandt KD. High power light emitting diode (LED) arrays versus halogen light polymerization of oral biomaterials: Barcol hardness, compressive strength and radiometric properties. Biomaterials. 2002;23:2955-2963
- 7. Wataha JC. Principles of biocompatibility for dental practitioners. J. Prosthet. Dent. 2001;86:203-209
- 8. Edgerton M, Levine MJ. Biocompatibility: its future in prosthodontic research. The J. Prosthet. Dent. 1993;69:406-415
- 9. Schmalz G, Schuster U, Koch A, Schweikl H. Cytotoxicity of low pH dentinbonding agents in a dentin barrier test in vitro. J. Endod. 2002;28:188-192
- 10. Sakaguchi RL. Craig's Restorative Dental Materials: Elsevier Health Sciences, 2006.
- 11. Zorba YO, Yıldız M. Adeziv Restoratif Materyallerde Biyouyumluluk Testleri Ve Kriterleri. J. Dent. Fac. Atatürk Uni.;2007.
- 12. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clin. Oral Investig. 1998;1:154-162.
- 13. Anonymous, ANSI/AAMI Standards and Recommended Practices. Biological evaluation of medical devices - ISO 10993, Parts 5 and 11, vol. 4, 1995.
- 14. Hosseinpour S, Gaudin A, Peters OA. A critical analysis of research methods and experimental models to study biocompatibility of endodontic materials. International Endodontic Journal. 2022 Apr;55:346-69.
- 15. Han X, Chen Y, Jiang Q, Liu X, Chen Y. Novel bioactive glass-modified hybrid composite resin: mechanical properties, biocompatibility, and antibacterial and remineralizing activity. Frontiers in Bioengineering and Biotechnology. 2021 Jun 1;9:661734.
- 16. Standardization IOF (2009). ISO 10993-5: Biological Evaluation of Medical Devices—Part 5: Tests for in Vitro Cytotoxicity. Geneva: International Organization for Standardization (ISO).
- 17. Standardization IOF (2012). ISO 10993-12: Biological Evaluation of Medical Devices—Part 12: Sample Preparation and Reference Materials. Geneva: International Organization for Standardization (ISO).
- 18. Repetto G, Del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature protocols. 2008 Jul;3(7):1125-31.
- 19. Standardization IOF (2011). ISO 7045: Pan Head Screws With Type H or Type Z Cross Recess—Product Grade A. Geneva: International Organization for Standardization (ISO).
- 20. EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing Version 12.0 (January 2024)
- 21. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 14.0, 2024.
- 22. Sürmelioğlu D, Hepokur C, Yavuz SA, Aydın U. Evaluation of the cytotoxic and genotoxic effects of different universal adhesive systems. Journal of Conservative Dentistry. 2020 Jul 1;23(4):384-9.
- 23. Eick JD, Kostoryz EL, Rozzi SM, Jacobs DW, Oxman JD, Chappelow CC, Glaros AG, Yourtee DM. In vitro biocompatibility of oxirane/polyol dental composites with promising physical properties. Dental Materials. 2002 Jul 1;18(5):413-21.
- 24. Kılıc K, Kesim B, Sumer Z, Polat Z, Ozturk A. Tam seramik materyallerinin biyouyumluluğunun MTT testi ile incelenmesi. J. Health Sci. 2010;19:125-132
- 25. Ruyter I. Physical and chemical aspects related to substances released from polymer materials in an aqueous environment. Adv. Dent. Res. 1995;9:344-347
- 26. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit. Rev. Oral Biol. Med. 2000;11:333-355
- 27. Hanks C, Strawn S, Watahai J, Craig R. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J. Dent. Res. 1991;70:1450-1455.
- 28. Al‐Dawood A, Wennberg A. Biocompatibility of dentin bonding agents. Endod. Dent. Traumatol. 1993;9:1-7
- 29. Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials: Springer, 2009
- 30. Schmalz G, Schuster U, Thonemann B, Barth M, Esterbauer S. Dentin barrier test with transfected bovine pulp-derived cells. J. Endod. 2001;27:96-102.
- 31. Jorge JH, Giampaolo ET, Vergani CE, Machado AL, Pavarina AC, Carlos IZ. Effect of post-polymerization heat treatments on the cytotoxicity of two denture base acrylic resins. J. Appl. Oral Sci. 2006;14:203-207
- 32. Schedle A, Franz A, Rausch-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dent. Mater. 1998;14:429-440
- 33. Samuelsen JT, Dahl JE, Karlsson S, Morisbak E, Becher R. Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK. Dent. Mater. 2007;23:34-39
- 34. Ratanasathien S, Wataha J, Hanks C, Dennison J. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J. Dent. Res. 1995;74:1602-1606
- 35. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J. Biomed. Mater. Res. 1997;37:517-524
- 36. Ergün G, Eğilmez F, Üçtaşli M, Yilmaz Ş. Effect of light curing type on cytotoxicity of dentine‐bonding agents. Int. Endod. J. 2007;40:216-223.
Investigation of Cytotoxic Effects and Antimicrobial Activities of Light-cured and Self-cured Universal Adhesive Systems
Abstract
Objective: This study aimed to compare the cytotoxicity and antimicrobial activity of a light-cured adhesive system and a self-cured adhesive system from the same company.
Materials and Methods: A Tokuyama BOND force II (Light-cured) adhesive system (TF2B) and a Tokuyama Universal Bond (Self-cured) adhesive system (TUB) were selected for the study. The cytotoxicity evaluation of these two systems on cell cultures was performed using MTT assay and Agar Diffusion assay in L929 fibroblast cells. Disk diffusion method and liquid microdilution (MIC) method were used to evaluate their antimicrobial activity. The experiments were performed on 6 pathogenic bacteria and 1 yeast fungus.
Results: According to MTT test results, both adhesive systems have no significant toxic effect on healthy cells (L929). However, when TUB and TF2B were compared with each other, it was found that TF2B had almost no toxic effect. In the agar diffusion test, when the two bonds were compared with each other, a weak color lightening was observed only around the first concentration of TUB. No visible melting was detected in other concentrations of TUB and TF2B. Both adhesive systems failed to reach MIC values effectively on the test microorganisms. Since the results were far above the MIC values of the reference antibiotics, it was determined that they did not have antimicrobial effects. Disk diffusion results similarly showed that both bonds did not form an inhibition zone on the test microorganisms.
Conclusions: In dentistry, cytotoxic effects of universal adhesive systems on living cells can be observed. Self-cured and Light-cured adhesive systems did not show toxic effects on L929 cells. In addition, antimicrobial effects on test microorganisms were not detected. The cytotoxicity of the materials can be tested on different cells.
References
- 1. Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J. Biomed. Mater. Res. 2008;86:523-529
- 2. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeek B, De Munck J. Bonding effectiveness of a new ‘multi-mode’adhesive to enamel and dentine. J. Dent. 2012;40:475-484
- 3. Perdigão J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multipurpose dentin adhesive. Am. J. Dent. 2012;25:153
- 4. Perdigão J, Muñoz MA, Sezinando A, Luque-Martinez IV, Staichak R, Reis A, Loguercio AD. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper. Dent. 2014;39:489-499
- 5. Caughman WF, Rueggeberg F. Shedding new light on composite polymerization. Oper. Dent. 2002;27:636-638
- 6. Mills RW, Uhl A, Blackwell GB, Jandt KD. High power light emitting diode (LED) arrays versus halogen light polymerization of oral biomaterials: Barcol hardness, compressive strength and radiometric properties. Biomaterials. 2002;23:2955-2963
- 7. Wataha JC. Principles of biocompatibility for dental practitioners. J. Prosthet. Dent. 2001;86:203-209
- 8. Edgerton M, Levine MJ. Biocompatibility: its future in prosthodontic research. The J. Prosthet. Dent. 1993;69:406-415
- 9. Schmalz G, Schuster U, Koch A, Schweikl H. Cytotoxicity of low pH dentinbonding agents in a dentin barrier test in vitro. J. Endod. 2002;28:188-192
- 10. Sakaguchi RL. Craig's Restorative Dental Materials: Elsevier Health Sciences, 2006.
- 11. Zorba YO, Yıldız M. Adeziv Restoratif Materyallerde Biyouyumluluk Testleri Ve Kriterleri. J. Dent. Fac. Atatürk Uni.;2007.
- 12. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clin. Oral Investig. 1998;1:154-162.
- 13. Anonymous, ANSI/AAMI Standards and Recommended Practices. Biological evaluation of medical devices - ISO 10993, Parts 5 and 11, vol. 4, 1995.
- 14. Hosseinpour S, Gaudin A, Peters OA. A critical analysis of research methods and experimental models to study biocompatibility of endodontic materials. International Endodontic Journal. 2022 Apr;55:346-69.
- 15. Han X, Chen Y, Jiang Q, Liu X, Chen Y. Novel bioactive glass-modified hybrid composite resin: mechanical properties, biocompatibility, and antibacterial and remineralizing activity. Frontiers in Bioengineering and Biotechnology. 2021 Jun 1;9:661734.
- 16. Standardization IOF (2009). ISO 10993-5: Biological Evaluation of Medical Devices—Part 5: Tests for in Vitro Cytotoxicity. Geneva: International Organization for Standardization (ISO).
- 17. Standardization IOF (2012). ISO 10993-12: Biological Evaluation of Medical Devices—Part 12: Sample Preparation and Reference Materials. Geneva: International Organization for Standardization (ISO).
- 18. Repetto G, Del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature protocols. 2008 Jul;3(7):1125-31.
- 19. Standardization IOF (2011). ISO 7045: Pan Head Screws With Type H or Type Z Cross Recess—Product Grade A. Geneva: International Organization for Standardization (ISO).
- 20. EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing Version 12.0 (January 2024)
- 21. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 14.0, 2024.
- 22. Sürmelioğlu D, Hepokur C, Yavuz SA, Aydın U. Evaluation of the cytotoxic and genotoxic effects of different universal adhesive systems. Journal of Conservative Dentistry. 2020 Jul 1;23(4):384-9.
- 23. Eick JD, Kostoryz EL, Rozzi SM, Jacobs DW, Oxman JD, Chappelow CC, Glaros AG, Yourtee DM. In vitro biocompatibility of oxirane/polyol dental composites with promising physical properties. Dental Materials. 2002 Jul 1;18(5):413-21.
- 24. Kılıc K, Kesim B, Sumer Z, Polat Z, Ozturk A. Tam seramik materyallerinin biyouyumluluğunun MTT testi ile incelenmesi. J. Health Sci. 2010;19:125-132
- 25. Ruyter I. Physical and chemical aspects related to substances released from polymer materials in an aqueous environment. Adv. Dent. Res. 1995;9:344-347
- 26. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit. Rev. Oral Biol. Med. 2000;11:333-355
- 27. Hanks C, Strawn S, Watahai J, Craig R. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J. Dent. Res. 1991;70:1450-1455.
- 28. Al‐Dawood A, Wennberg A. Biocompatibility of dentin bonding agents. Endod. Dent. Traumatol. 1993;9:1-7
- 29. Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials: Springer, 2009
- 30. Schmalz G, Schuster U, Thonemann B, Barth M, Esterbauer S. Dentin barrier test with transfected bovine pulp-derived cells. J. Endod. 2001;27:96-102.
- 31. Jorge JH, Giampaolo ET, Vergani CE, Machado AL, Pavarina AC, Carlos IZ. Effect of post-polymerization heat treatments on the cytotoxicity of two denture base acrylic resins. J. Appl. Oral Sci. 2006;14:203-207
- 32. Schedle A, Franz A, Rausch-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dent. Mater. 1998;14:429-440
- 33. Samuelsen JT, Dahl JE, Karlsson S, Morisbak E, Becher R. Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK. Dent. Mater. 2007;23:34-39
- 34. Ratanasathien S, Wataha J, Hanks C, Dennison J. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J. Dent. Res. 1995;74:1602-1606
- 35. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J. Biomed. Mater. Res. 1997;37:517-524
- 36. Ergün G, Eğilmez F, Üçtaşli M, Yilmaz Ş. Effect of light curing type on cytotoxicity of dentine‐bonding agents. Int. Endod. J. 2007;40:216-223.
Details
| Primary Language | English |
|---|---|
| Subjects | Restorative Dentistry, Dental Materials, Dentistry (Other) |
| Journal Section | Original Research Articles |
| Authors | |
| Publication Date | September 30, 2024 |
| Submission Date | July 16, 2024 |
| Acceptance Date | August 11, 2024 |
| Published in Issue | Year 2024Volume: 27 Issue: 3 |
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