Research Article
BibTex RIS Cite

The effect of minimally invasive treatments on enamel microhardness and resistance to further demineralization

Year 2022, Volume: 25 Issue: 4, 285 - 290, 30.12.2022

Horieh Moosavi Fatemeh Rezaee Safoora Afshari Salehe Sekandari , Farzaneh Ahrari

https://doi.org/10.7126/cumudj.1106247

Abstract

Objectives: The present study aimed to compare microhardness of inactive proximal lesions treated by resin infiltration, Er:YAG laser + resin infiltration and Bioactive glass, and investigate the resistance of treated lesions to further demineralization challenge.
Methods: In this in-vitro study, 30 human molars with inactive proximal lesions were selected and randomly divided into three groups of 10. In group 1 (resin infiltration), the lesions were treated by a resin infiltrant (Icon). In group 2, the surface was conditioned by an Er:YAG laser prior to resin infiltration. The specimens in group 3 were remineralized by bioactive glass. The treated specimens were kept in artificial saliva for 1 week and then immersed in a demineralization solution for 8 weeks. Surface microhardness was measured at baseline (T0), after remineralization (T1) and after exposure to the demineralization solution (T2), and the difference in microhardness between time points (ΔVHN) was calculated.
Results: Microhardness after demineralization (T2) was significantly lower than those of other intervals (P<0.05), but no significant difference was found between the T0 and T1 values (P>0.05). The statistical analysis revealed no significant difference either in ΔVHNT1-T0 or in ΔVHNT2-T1 among the study groups (P>0.05)
Conclusion: Pretreatment by the Er:YAG laser prior to resin infiltration was more effective that other treatments in enhancing microhardness and protecting the tooth against acidic challenge. However, the difference between groups did not reach a statistical significance, implying the need for further studies to achieve more conclusive results.

Keywords

proximal caries remineralization Bioactive glass Er:YAG laser resin infiltration

Supporting Institution

Mashhad University of Medical Sciences

Project Number

940325

References

  • 1.El Meligy OA, Alamoudi NM, Ibrahim ST, Felemban OM, Al-Tuwirqi AA. Effect of resin infiltration application on early proximal caries lesions in vitro. Journal of dental sciences. 2021 Jan 1;16(1):296-303.
  • 2.Braga MM, Mendes FM, Ekstrand KR. Detection activity assessment and diagnosis of dental caries lesions. Dental Clinics of North America. 2010;54:479-93.
  • 3.Splieth CH, Kanzow P, Wiegand A, Schmoeckel J, Jablonski-Momeni A. How to intervene in the caries process: proximal caries in adolescents and adults-a systematic review and meta-analysis. Clin Oral Investig. 2020;24:1623-1636.
  • 4.Ahrari F, Akbari M, Mohammadi M, Fallahrastegar A, Najafi MN. The validity of laser fluorescence (LF) and near-infrared reflection (NIRR) in detecting early proximal cavities. Clin Oral Investig 2021;25(8):4817-24.
  • 5.Dai LL, Mei ML, Chu CH, Lo ECM. Mechanisms of Bioactive Glass on Caries Management: A Review. Materials (Basel). 2019;12(24):4183.
  • 6. Manoharan V, Arun Kumar S, Arumugam SB, Anand V, Krishnamoorthy S, Methippara JJ. Is Resin Infiltration a Microinvasive Approach to White Lesions of Calcified Tooth Structures?: A Systemic Review. Int J Clin Pediatr Dent. 2019;12:53-58.
  • 7.Liang Y, Deng Z, Dai X, Tian J, Zhao W. Micro-invasive interventions for managing non-cavitated proximal caries of different depths: a systematic review and meta-analysis. Clin Oral Investig. 2018;22:2675–84.
  • 8.Dorri M, Dunne SM, Walsh T, Schwendicke F. Micro-invasive interventions for managing proximal dental decay in primary and permanent teeth. Cochrane Database Syst Rev. 2015;2015:CD010431.
  • 9.Krois J, Göstemeyer G, Reda S, Schwendicke F. Sealing or infiltrating proximal carious lesions [published correction appears in J Dent. 2018 Sep;76:137-138]. J Dent. 2018;74:15-22.
  • 10.Ammari MM, Soviero VM, da Silva Fidalgo TK, et al. Is non-cavitated proximal lesion sealing an effective method for caries control in primary and permanent teeth? A systematic review and meta-analysis. J Dent. 2014;42:1217-1227.
  • 11.Chatzimarkou S, Koletsi D, Kavvadia K. The effect of resin infiltration on proximal caries lesions in primary and permanent teeth. A systematic review and meta-analysis of clinical trials. J Dent. 2018;77:8–17.
  • 12. Moosavi H, Ahrari F, Zanjani M. Effect of Er:YAG laser radiation on pull-out fracture load of esthetic posts luted to root canal dentin with various resin cements. Dent Res J (Isfahan) 2021;18:17.
  • 13.Moosavi H, Ghorbanzadeh S, Ahrari F. Structural and Morphological Changes in Human Dentin after Ablative and Subablative Er:YAG Laser Irradiation. J Lasers Med Sci 2016;7:86-91.
  • 14.Heravi F, Ahrari F, Mahdavi M, Basafa S. Comparative evaluation of the effect of Er:YAG laser and low level laser irradiation combined with CPP-ACPF cream on treatment of enamel caries. J Clin Exp Dent 2014;6(2):e121-6.
  • 15.Ahrari F, Poosti M, Motahari P. Enamel resistance to demineralization following Er:YAG laser etching for bonding orthodontic brackets. Dent Res J (Isfahan) 2012;9(4):472-7.
  • 16.Dehghani M, Ahrari F. The effect of surface treatment with Er: YAG laser on shear bond strength of orthodontic brackets to fiber-reinforced composite. J Clin Exp Dent 2014;6(4):e379-83.
  • 17.Zhang Y, Wang Y, Chen Y, Chen Y, Zhang Q, Zou J. The clinical effects of laser preparation of tooth surfaces for fissure sealants placement: a systematic review and meta-analysis. BMC Oral Health. 2019;19(1):1-12.
  • 18.Jones JR. Review of bioactive glass: from Hench to hybrids. Acta biomaterialia. 2013;9:4457-86.
  • 19.DAI JM, LI FH, Qiu XL, DONG X. Effects of Rein Infiltration and Bioactive Glass on Early Caries. Journal of Oral Science Research. 2019 May 28;35(5):453-6.
  • 20.Torres CR, Rosa PC, Ferreira NS, Borges AB. Effect of caries infiltration technique and fluoride therapy on microhardness of enamel carious lesions. Oper Dent. 2012;37:363-369.
  • 21.Paris S, Meyer-Lueckel H, Cölfen H, Kielbassa AM. Resin infiltration of artificial enamel caries lesions with experimental light curing resins. Dent Mater J. 2007;26:582-8.
  • 22.Ahrari F, Jalaly T, Zebarjad M. Tensile properties of orthodontic elastomeric ligatures. Indian J Dent Res 2010;21(1):23-9.
  • 23.Ghanbarzadeh M, Ahrari F, Akbari M, Hamzei H. Microhardness of demineralized enamel following home bleaching and laser-assisted in office bleaching. J Clin Exp Dent 2015;7:e405-9.
  • 24.Ahrari F, Hasanzadeh N, Rajabi O, Forouzannejad Z. Effectiveness of sodium bicarbonate combined with hydrogen peroxide and CPP-ACPF in whitening and microhardness of enamel. J Clin Exp Dent 2017;9(3):e344-e50.
  • 25.Paris S, Schwendicke F, Seddig S, MüllerW-D, Dörfer C, Meyer-Lueckel H. Micro-hardness and mineral loss of enamel lesions after infiltration with various resins: Influence of infiltrant composition and application frequency in vitro. J Dent. 2013;41:543-8.
  • 26.OAESE M, Ibrahim ST, Alamoudi NM. Resin infiltration of non-cavitated proximal caries lesions: a literature review. J Oral Hyg Health. 2018;6(1):235-43.
  • 27.Paris S, Meyer-Lueckel H. Inhibition of caries progression by resin infiltration in situ. Caries research. 2010;44(1):47-54.
  • 28.Polat YK, Ilday NO. The effects of different anticavity agents and Er: YAG laser usage on enamel surface microhardness. Oral Health Prev Dent. 2020;18(1):601-6.
  • 29.Ahrari F, Basafa M, Fekrazad R, Mokarram M, Akbari M. The efficacy of Er,Cr:YSGG laser in reconditioning of metallic orthodontic brackets. Photomed Laser Surg 2012;30:41-6.
  • 30.Zarabian T, Azadi Mood S, Kiomarsi N, Noorollahian H, Hakimiha N. Microshear Bond Strength of a Self-adhesive Composite to Erbium Laser-Treated Primary Enamel. J Lasers Med Sci. 2020;11:181-186.
  • 31.Manhart J, Huth KC, Chen HY, Hickel R. Influence of the pretreatment of occlusal pits and fissures on the retention of a fissure sealant. Am J Dent. 2004;17:12-18.
  • 32.Lupi-Pegurier L, Bertrand MF, Muller-Bolla M, Rocca JP, Bolla M. Comparative study of microleakage of a pit and fissure sealant placed after preparation by Er:YAG laser in permanent molars. J Dent Child (Chic). 2003;70:134-8.
  • 33.Ahrari F, Fekrazad R, Kalhori KA, Ramtin M. Reconditioning of ceramic orthodontic brackets with an Er,Cr:YSGG laser. Lasers Med Sci 2013;28:223-8.
  • 34.Dehghani M, Ahrari F. The effect of surface treatment with Er: YAG laser on shear bond strength of orthodontic brackets to fiber-reinforced composite. J Clin Exp Dent 2014;6:e379-83.
  • 35.Xu YT, Wu Q, Chen YM, Smales RJ, Shi SY, Wang MT. Antimicrobial effects of a bioactive glass combined with fluoride or triclosan on Streptococcus mutans biofilm. Arch Oral Biol. 2015;60:1059-65.
  • 36.Geraldo-Martins VR, Lepri CP, Palma-Dibb RG. Influence of Er,Cr:YSGG laser irradiation on enamel caries prevention. Lasers Med Sci. 2013;28:33-9.
  • 37.Ali, S.; Farooq, I.; Iqbal, K. A review of the effect of various ions on the properties and the clinical applications of novel bioactive glasses in medicine and dentistry. Saudi Dent. J. 2014, 26, 1–5.
  • 38.Qi Y-p, Li N, Niu L-n, Primus CM, Ling J-Q, Pashley DH, et al. Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate. Acta Biomaterialia. 2012;8:836-42.
  • 39.Ebrahimi M, Mehrabkhani M, Ahrari F, Parisay I, Jahantigh M. The effects of three remineralizing agents on regression of white spot lesions in children: A two-week, single-blind, randomized clinical trial. J Clin Exp Dent 2017;9:e641-e48.
  • 40.Heravi F, Ahrari F, Tanbakuchi B. Effectiveness of MI Paste Plus and Remin Pro on remineralization and color improvement of postorthodontic white spot lesions. Dent Res J (Isfahan) 2018;15:95-103.
  • 41.Moghaddas MJ, Moosavi H, Yaghoubirad S, Chiniforush N. The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study. J Lasers Med Sci. 2020;11(2):160-166.
  • 42.Kim HJ, Mo SY, Kim DS. Effect of bioactive glass-containing light-curing varnish on enamel remineralization. Materials. 2021;14(13):3745.
  • 43.Dai LL, Mei ML, Chu CH, Lo ECM. Remineralizing effect of a new strontium-doped bioactive glass and fluoride on demineralized enamel and dentine. J Dent. 2021;108:103633.
  • 44.Sharda S, Gupta A, Goyal A, Gauba K. Remineralization potential and caries preventive efficacy of CPP-ACP/Xylitol/Ozone/Bioactive glass and topical fluoride combined therapy versus fluoride mono-therapy - a systematic review and meta-analysis. Acta Odontol Scand. 2021;79:402-417.

Year 2022, Volume: 25 Issue: 4, 285 - 290, 30.12.2022

Horieh Moosavi Fatemeh Rezaee Safoora Afshari Salehe Sekandari , Farzaneh Ahrari

https://doi.org/10.7126/cumudj.1106247

Abstract

Project Number

940325

References

  • 1.El Meligy OA, Alamoudi NM, Ibrahim ST, Felemban OM, Al-Tuwirqi AA. Effect of resin infiltration application on early proximal caries lesions in vitro. Journal of dental sciences. 2021 Jan 1;16(1):296-303.
  • 2.Braga MM, Mendes FM, Ekstrand KR. Detection activity assessment and diagnosis of dental caries lesions. Dental Clinics of North America. 2010;54:479-93.
  • 3.Splieth CH, Kanzow P, Wiegand A, Schmoeckel J, Jablonski-Momeni A. How to intervene in the caries process: proximal caries in adolescents and adults-a systematic review and meta-analysis. Clin Oral Investig. 2020;24:1623-1636.
  • 4.Ahrari F, Akbari M, Mohammadi M, Fallahrastegar A, Najafi MN. The validity of laser fluorescence (LF) and near-infrared reflection (NIRR) in detecting early proximal cavities. Clin Oral Investig 2021;25(8):4817-24.
  • 5.Dai LL, Mei ML, Chu CH, Lo ECM. Mechanisms of Bioactive Glass on Caries Management: A Review. Materials (Basel). 2019;12(24):4183.
  • 6. Manoharan V, Arun Kumar S, Arumugam SB, Anand V, Krishnamoorthy S, Methippara JJ. Is Resin Infiltration a Microinvasive Approach to White Lesions of Calcified Tooth Structures?: A Systemic Review. Int J Clin Pediatr Dent. 2019;12:53-58.
  • 7.Liang Y, Deng Z, Dai X, Tian J, Zhao W. Micro-invasive interventions for managing non-cavitated proximal caries of different depths: a systematic review and meta-analysis. Clin Oral Investig. 2018;22:2675–84.
  • 8.Dorri M, Dunne SM, Walsh T, Schwendicke F. Micro-invasive interventions for managing proximal dental decay in primary and permanent teeth. Cochrane Database Syst Rev. 2015;2015:CD010431.
  • 9.Krois J, Göstemeyer G, Reda S, Schwendicke F. Sealing or infiltrating proximal carious lesions [published correction appears in J Dent. 2018 Sep;76:137-138]. J Dent. 2018;74:15-22.
  • 10.Ammari MM, Soviero VM, da Silva Fidalgo TK, et al. Is non-cavitated proximal lesion sealing an effective method for caries control in primary and permanent teeth? A systematic review and meta-analysis. J Dent. 2014;42:1217-1227.
  • 11.Chatzimarkou S, Koletsi D, Kavvadia K. The effect of resin infiltration on proximal caries lesions in primary and permanent teeth. A systematic review and meta-analysis of clinical trials. J Dent. 2018;77:8–17.
  • 12. Moosavi H, Ahrari F, Zanjani M. Effect of Er:YAG laser radiation on pull-out fracture load of esthetic posts luted to root canal dentin with various resin cements. Dent Res J (Isfahan) 2021;18:17.
  • 13.Moosavi H, Ghorbanzadeh S, Ahrari F. Structural and Morphological Changes in Human Dentin after Ablative and Subablative Er:YAG Laser Irradiation. J Lasers Med Sci 2016;7:86-91.
  • 14.Heravi F, Ahrari F, Mahdavi M, Basafa S. Comparative evaluation of the effect of Er:YAG laser and low level laser irradiation combined with CPP-ACPF cream on treatment of enamel caries. J Clin Exp Dent 2014;6(2):e121-6.
  • 15.Ahrari F, Poosti M, Motahari P. Enamel resistance to demineralization following Er:YAG laser etching for bonding orthodontic brackets. Dent Res J (Isfahan) 2012;9(4):472-7.
  • 16.Dehghani M, Ahrari F. The effect of surface treatment with Er: YAG laser on shear bond strength of orthodontic brackets to fiber-reinforced composite. J Clin Exp Dent 2014;6(4):e379-83.
  • 17.Zhang Y, Wang Y, Chen Y, Chen Y, Zhang Q, Zou J. The clinical effects of laser preparation of tooth surfaces for fissure sealants placement: a systematic review and meta-analysis. BMC Oral Health. 2019;19(1):1-12.
  • 18.Jones JR. Review of bioactive glass: from Hench to hybrids. Acta biomaterialia. 2013;9:4457-86.
  • 19.DAI JM, LI FH, Qiu XL, DONG X. Effects of Rein Infiltration and Bioactive Glass on Early Caries. Journal of Oral Science Research. 2019 May 28;35(5):453-6.
  • 20.Torres CR, Rosa PC, Ferreira NS, Borges AB. Effect of caries infiltration technique and fluoride therapy on microhardness of enamel carious lesions. Oper Dent. 2012;37:363-369.
  • 21.Paris S, Meyer-Lueckel H, Cölfen H, Kielbassa AM. Resin infiltration of artificial enamel caries lesions with experimental light curing resins. Dent Mater J. 2007;26:582-8.
  • 22.Ahrari F, Jalaly T, Zebarjad M. Tensile properties of orthodontic elastomeric ligatures. Indian J Dent Res 2010;21(1):23-9.
  • 23.Ghanbarzadeh M, Ahrari F, Akbari M, Hamzei H. Microhardness of demineralized enamel following home bleaching and laser-assisted in office bleaching. J Clin Exp Dent 2015;7:e405-9.
  • 24.Ahrari F, Hasanzadeh N, Rajabi O, Forouzannejad Z. Effectiveness of sodium bicarbonate combined with hydrogen peroxide and CPP-ACPF in whitening and microhardness of enamel. J Clin Exp Dent 2017;9(3):e344-e50.
  • 25.Paris S, Schwendicke F, Seddig S, MüllerW-D, Dörfer C, Meyer-Lueckel H. Micro-hardness and mineral loss of enamel lesions after infiltration with various resins: Influence of infiltrant composition and application frequency in vitro. J Dent. 2013;41:543-8.
  • 26.OAESE M, Ibrahim ST, Alamoudi NM. Resin infiltration of non-cavitated proximal caries lesions: a literature review. J Oral Hyg Health. 2018;6(1):235-43.
  • 27.Paris S, Meyer-Lueckel H. Inhibition of caries progression by resin infiltration in situ. Caries research. 2010;44(1):47-54.
  • 28.Polat YK, Ilday NO. The effects of different anticavity agents and Er: YAG laser usage on enamel surface microhardness. Oral Health Prev Dent. 2020;18(1):601-6.
  • 29.Ahrari F, Basafa M, Fekrazad R, Mokarram M, Akbari M. The efficacy of Er,Cr:YSGG laser in reconditioning of metallic orthodontic brackets. Photomed Laser Surg 2012;30:41-6.
  • 30.Zarabian T, Azadi Mood S, Kiomarsi N, Noorollahian H, Hakimiha N. Microshear Bond Strength of a Self-adhesive Composite to Erbium Laser-Treated Primary Enamel. J Lasers Med Sci. 2020;11:181-186.
  • 31.Manhart J, Huth KC, Chen HY, Hickel R. Influence of the pretreatment of occlusal pits and fissures on the retention of a fissure sealant. Am J Dent. 2004;17:12-18.
  • 32.Lupi-Pegurier L, Bertrand MF, Muller-Bolla M, Rocca JP, Bolla M. Comparative study of microleakage of a pit and fissure sealant placed after preparation by Er:YAG laser in permanent molars. J Dent Child (Chic). 2003;70:134-8.
  • 33.Ahrari F, Fekrazad R, Kalhori KA, Ramtin M. Reconditioning of ceramic orthodontic brackets with an Er,Cr:YSGG laser. Lasers Med Sci 2013;28:223-8.
  • 34.Dehghani M, Ahrari F. The effect of surface treatment with Er: YAG laser on shear bond strength of orthodontic brackets to fiber-reinforced composite. J Clin Exp Dent 2014;6:e379-83.
  • 35.Xu YT, Wu Q, Chen YM, Smales RJ, Shi SY, Wang MT. Antimicrobial effects of a bioactive glass combined with fluoride or triclosan on Streptococcus mutans biofilm. Arch Oral Biol. 2015;60:1059-65.
  • 36.Geraldo-Martins VR, Lepri CP, Palma-Dibb RG. Influence of Er,Cr:YSGG laser irradiation on enamel caries prevention. Lasers Med Sci. 2013;28:33-9.
  • 37.Ali, S.; Farooq, I.; Iqbal, K. A review of the effect of various ions on the properties and the clinical applications of novel bioactive glasses in medicine and dentistry. Saudi Dent. J. 2014, 26, 1–5.
  • 38.Qi Y-p, Li N, Niu L-n, Primus CM, Ling J-Q, Pashley DH, et al. Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate. Acta Biomaterialia. 2012;8:836-42.
  • 39.Ebrahimi M, Mehrabkhani M, Ahrari F, Parisay I, Jahantigh M. The effects of three remineralizing agents on regression of white spot lesions in children: A two-week, single-blind, randomized clinical trial. J Clin Exp Dent 2017;9:e641-e48.
  • 40.Heravi F, Ahrari F, Tanbakuchi B. Effectiveness of MI Paste Plus and Remin Pro on remineralization and color improvement of postorthodontic white spot lesions. Dent Res J (Isfahan) 2018;15:95-103.
  • 41.Moghaddas MJ, Moosavi H, Yaghoubirad S, Chiniforush N. The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study. J Lasers Med Sci. 2020;11(2):160-166.
  • 42.Kim HJ, Mo SY, Kim DS. Effect of bioactive glass-containing light-curing varnish on enamel remineralization. Materials. 2021;14(13):3745.
  • 43.Dai LL, Mei ML, Chu CH, Lo ECM. Remineralizing effect of a new strontium-doped bioactive glass and fluoride on demineralized enamel and dentine. J Dent. 2021;108:103633.
  • 44.Sharda S, Gupta A, Goyal A, Gauba K. Remineralization potential and caries preventive efficacy of CPP-ACP/Xylitol/Ozone/Bioactive glass and topical fluoride combined therapy versus fluoride mono-therapy - a systematic review and meta-analysis. Acta Odontol Scand. 2021;79:402-417.
There are 44 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research Articles
Authors

Horieh Moosavi 0000-0001-7386-457X

Fatemeh Rezaee 0000-0003-1078-0576

Safoora Afshari 0000-0003-4540-9400

Salehe Sekandari 0000-0001-8190-915X

Farzaneh Ahrari 0000-0003-1257-8881

Project Number 940325
Publication Date December 30, 2022
Submission Date May 6, 2022
Published in Issue Year 2022Volume: 25 Issue: 4

Cite

EndNote Moosavi H, Rezaee F, Afshari S, Sekandari S, Ahrari F (December 1, 2022) The effect of minimally invasive treatments on enamel microhardness and resistance to further demineralization. Cumhuriyet Dental Journal 25 4 285–290.
 Download Cover Image

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


CDJ accepts articles in English. Submitting a paper to CDJ is free of charges. In addition, CDJ has not have article processing charges.

Frequency: Four times a year (March, June, September, and December)

IMPORTANT NOTICE

All users of Cumhuriyet Dental Journal should visit to their user's home page through the "https://dergipark.org.tr/tr/user" " or "https://dergipark.org.tr/en/user" links to update their incomplete information shown in blue or yellow warnings and update their e-mail addresses and information to the DergiPark system. Otherwise, the e-mails from the journal will not be seen or fall into the SPAM folder. Please fill in all missing part in the relevant field.

Please visit journal's AUTHOR GUIDELINE to see revised policy and submission rules to be held since 2020.