ASSESSMENT OF DMFT INDEXES, SALIVARY FLOW RATE, PH, AND DETECTIONS OF S.MUTANS SALIVARY LEVELS BY A QUANTITATIVE REAL-TIME PCR IN POLYCYSTIC OVARY SYNDROME

N. Ezgi Yeniçeri Hilaloğlu; Derya Gursel Surmelioglu

doi:10.7126/cumudj.1132273

Research Article

Year 2022, Volume: 25 Issue: 2, 163 - 171, 30.06.2022

N. Ezgi Yeniçeri Hilaloğlu , Derya Gursel Surmelioglu

https://doi.org/10.7126/cumudj.1132273

Abstract

Supporting Institution

Gaziantep Universitesi Bilimsel Araştırmalar Projeler Birimi

Project Number

DHF.UT.19.04.

References

1. Barber TM, Franks S. Obesity and polycystic ovary syndrome. Clin. Endocrinol. 2021;95(4):531-541.
2. Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. The Lancet. 2007;370(9588):685-697.
3. Indhavivadhana S, Kuichanuan M, Wongwananuruk T, Techatraisak K, Chantrapanichkul P, Dangrat C. Correlation of Hyperandrogenemia and Metabolic Syndrome in Thai Women With Polycystic Ovary Syndrome (PCOS). 2021; 1-12.
4. Fulghesu AM, Piras C, Dessì A, et al. Urinary Metabolites Reveal Hyperinsulinemia and Insulin Resistance in Polycystic Ovarian Syndrome (PCOS). Metabolites. 2021;11(7):437.
5. Dursun E, Akalın FA, Güncü GN, et al. Periodontal disease in polycystic ovary syndrome. Fertil. Steril. 2011;95(1):320-323.
6. Kassebaum N, Bernabé E, Dahiya M, Bhandari B, Murray C, Marcenes W. Global burden of severe periodontitis in 1990-2010: a systematic review and meta-regression. J. Dent. Res. 2014;93(11):1045-1053.
7. Cardoso EM, Reis C, Manzanares-Céspedes MC. Chronic periodontitis, inflammatory cytokines, and interrelationship with other chronic diseases. Postgrad Med. 2018;130(1):98-104.
8. Bowen W, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011;45(1):69-86.
9. Koo H, Falsetta M, Klein M. The exopolysaccharide matrix: a virulence determinant of cariogenic biofilm. J. Dent. Res. 2013;92(12):1065-1073.
10. Motallaei MN, Yazdanian M, Tebyanian H, et al. The Current Strategies in Controlling Oral Diseases by Herbal and Chemical Materials. Evid Based Complementary Altern Med. 2021;2021
11. Arce J-C, Smitz J. Exogenous hCG activity, but not endogenous LH activity, is positively associated with live birth rates in anovulatory infertility. Hum Fertil. 2011;14(3):192-199.
12. Solorzano CMB, Beller JP, Abshire MY, Collins JS, McCartney CR, Marshall JC. Neuroendocrine dysfunction in polycystic ovary syndrome. Steroids. 2012;77(4):332-337.
13. Tedjosasongko U, Ramadhaniati DM, Pradopo S. Streptococcus mutans detection on mother-child pairs using matrix-assisted laser desorption ionization–time of flight mass spectrometry and polymerase chain reaction. Dental Journal (Majalah Kedokteran Gigi). 2021;54(1):52-56.
14. Al-Blooshi SY, Latif MAA, Sabaneh NK, Mgaogao M, Hossain A. Development of a novel selective medium for culture of Gram-negative bacteria. BMC Res Notes. 2021;14(1):1-6.
15. Ethirajulu A, Alkasabera A, Onyali CB, et al. Insulin Resistance, Hyperandrogenism, and Its Associated Symptoms Are the Precipitating Factors for Depression in Women With Polycystic Ovarian Syndrome. Cureus. 2021;13(9)
16. Szczuko M, Kikut J, Szczuko U, et al. Nutrition strategy and life style in polycystic ovary syndrome—Narrative review. Nutrients. 2021;13(7):2452.
17. Isola G. Advances in biomarkers and diagnostics in periodontitis and oral diseases. Multidisciplinary Digital Publishing Institute; 2021.
18. Aiuchi H, Kitasako Y, Fukuda Y, Nakashima S, Burrow M, Tagami J. Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential. Aust. Dent. J.2008;53(2):167-171.
19. Radović K, Brković B, Roganović J, Ilić J, Milić Lemić A, Jovanović B. Salivary VEGF and post-extraction wound healing in type 2 diabetic immediate denture wearers. Acta Odontol. Scand.2021:1-6.
20. Zhang X, Wang M, Wang X, et al. Relationship between periodontitis and microangiopathy in type 2 diabetes mellitus: a meta‐analysis. J. Periodontal Res.2021; 56:1019–1027.
21. Malicka B, Kaczmarek U, Skośkiewicz-Malinowska K. Prevalence of xerostomia and the salivary flow rate in diabetic patients. Adv Clin Exp Med 2014;23(2):225-233.
22. Boyce HW, Bakheet MR. Sialorrhea: a review of a vexing, often unrecognized sign of oropharyngeal and esophageal disease. J. Clin. Gastroenterol 2005;39(2):89-97.
23. Saleh J, Figueiredo MAZ, Cherubini K, Salum FG. Salivary hypofunction: an update on aetiology, diagnosis and therapeutics. Arch. Oral Biol 2015;60(2):242-255.
24. da Mata ADSP, Amaral JPdAR, Thomson WM, et al. Patient‐related outcomes in Sjögren syndrome treated with stimulants of salivary secretion: Randomized clinical trial. Oral Dis. 2020;26(2):313-324.
25. Özcan E, Bulut İ, Berk S, Çanakçı CF. Astımlı hastalarda kısa ve uzun dönem inhaler kortikosteroid kullanımının oral ve periodontal sağlık üzerine etkileri. 2011; 13(2): 16-22.
26. Mumcu G, Ergun T, Inanc Na, et al. Oral health is impaired in Behcet's disease and is associated with disease severity. Rheumatology. 2004;43(8):1028-1033.
27. Loyola Rodriguez J, Galvan Torres L, Martinez Martinez R, et al. Frequency of dental caries in active and inactive systemic lupus erythematous patients: salivary and bacterial factors. Lupus. 2016;25(12):1349-1356.
28. Russell SL, Mayberry LJ. Pregnancy and oral health: a review and recommendations to reduce gaps in practice and research. MCN Am J Matern Child Nurs . 2008;33(1):32-37.
29. Rajesh K, Zareena SH, Kumar MA. Assessment of salivary calcium, phosphate, magnesium, pH, and flow rate in healthy subjects, periodontitis, and dental caries. Contemp. Clin. Dent." 2015;6(4):461.
30. Erdem V, Yıldız M, Erdem T. The evaluation of saliva flow rate, pH, buffer capacity, microbiological content and indice of decayed, missing and filled teeth in behçet’s patients. Balkan Med J. 2013;30(2):211.
31. Curtis MA, Diaz PI, Van Dyke TE. The role of the microbiota in periodontal disease. Periodontol 2000. 2020;83(1):14-25.
32. Nair SD, Varma S, Suragimath G, Zope S, Kale V, Abbayya K. Prevalence of periodontal disease in women with polycystic ovary syndrome-a comparative descriptive study. J Evol Med Dent Sci. 2017;6:4733-4736.
33. Porwal S, Tewari S, Sharma RK, Singhal SR, Narula SC. Periodontal status and high‐sensitivity C‐reactive protein levels in polycystic ovary syndrome with and without medical treatment. J. Periodontol.2014;85(10):1380-1389.
34. Surmelioglu D, Yeniceri E, İsman O, Gunduz R. Evaluation Of Saliva Analysis And Dmft Indexs In Polycystic Ovary Syndrome. Akd Med J 2020;6:346-350.
35. Siudikiene J, Machiulskiene V, Nyvad B, Tenovuo J, Nedzelskiene I. Dental caries and salivary status in children with type 1 diabetes mellitus, related to the metabolic control of the disease. Eur. J. Oral Sci. 2006;114(1):8-14.
36. Nanda J, Sachdev V, Sandhu M, Deep-Singh-Nanda K. Correlation between dental caries experience and mutans streptococci counts using saliva and plaque as microbial risk indicators in 3-8 year old children. A cross sectional study. J Clin Exp Dent 2015;7(1):e114.
37. Rodrigues MJ, Menezes VA, Marques KMG, Santos FAd. Caries prevalence and socioeconomic factors in children with sickle cell anemia. Braz. Oral Res 2012;26(1):43-49.
38. Erdem V, Yildiz M, Erdem T. The Evaluation of Saliva Flow Rate. Balkan Med J. 2013;30(2)
39. Glodny B, Nasseri P, Crismani A, et al. The occurrence of dental caries is associated with atherosclerosis. Clinics. 2013;68(7):946-953.
40. Silva AER, Menezes AMB, Demarco FF, Vargas-Ferreira F, Peres MA. Obesity and dental caries: systematic review. Rev Saude Publica 013;47:799-812.
41. Syrjälä A-MH, Niskanen MC, Ylöstalo P, Knuuttila ML. Metabolic control as a modifier of the association between salivary factors and dental caries among diabetic patients. Caries Res. 2003;37(2):142-147.
42. Nakahara Y, Sano T, Kodama Y, Ozaki K, Matsuura T. Alloxan-induced hyperglycemia causes rapid-onset and progressive dental caries and periodontitis in F344 rats. Histol Histopathol 2012; 27: 1297-1306. 43. Bowden G. The microbial ecology of dental caries. Microb Ecol Health Dis. 2000;12(3):138-148.

ASSESSMENT OF DMFT INDEXES, SALIVARY FLOW RATE, PH, AND DETECTIONS OF S.MUTANS SALIVARY LEVELS BY A QUANTITATIVE REAL-TIME PCR IN POLYCYSTIC OVARY SYNDROME

Year 2022, Volume: 25 Issue: 2, 163 - 171, 30.06.2022

N. Ezgi Yeniçeri Hilaloğlu , Derya Gursel Surmelioglu

https://doi.org/10.7126/cumudj.1132273

Abstract

Objectives: PCOS is an endocrine disorder that is common in women. However, PCOS effects on oral and dental health have not been stated clearly. The aim of this study is to examine the effects of Polycystic Ovary Syndrome (PCOS), which is common in women of reproductive age, on saliva and dental tissues in these women.
Materials and Methods: One-hundred individuals who were / were not diagnosed with PCOS and insulin resistance were included in this study (n=100). Subsequently, individuals, with PCOS and insulin resistance (PCOSID +), with PCOS and non-insulin resistance (PCOSID-), without PCOS and insulin resistance (ControlID +) and without PCOS and non-insulin resistance (ControlID-) were divided into 4 groups (n=25). DMFT (Decayed, Missing, Filled Teeth) index was used for dental health evaluation, while pH meter was used for saliva pH measurement. Also, Streptococcus Mutans (S. Mutans) numbers were analyzed by the real-time Polymerase Chain Reaction (PCR) method. In statistical analysis p<0.05 was considered significant.
Results: In comparison among the groups, significant differences were found in terms of DMFT index, S. Mutans, and salivary pH values (p<0.05). Among the compared groups, the highest DMFT index, S. Mutans values were found in the PCOSID(+) group, the lowest in ControlID(-) group, while the lowest saliva pH value was found in the PCOSID(+) group.
Conclusions: S. Mutans and DMFT index values were found highly in the saliva of PCOS patients, which is a multifactorial syndrome, and it is determined that salivary parameters have an effect on this situation.

Keywords

PCOS, real-time PCR, S. Mutans, salivary pH

Project Number

DHF.UT.19.04.

References

1. Barber TM, Franks S. Obesity and polycystic ovary syndrome. Clin. Endocrinol. 2021;95(4):531-541.
2. Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. The Lancet. 2007;370(9588):685-697.
3. Indhavivadhana S, Kuichanuan M, Wongwananuruk T, Techatraisak K, Chantrapanichkul P, Dangrat C. Correlation of Hyperandrogenemia and Metabolic Syndrome in Thai Women With Polycystic Ovary Syndrome (PCOS). 2021; 1-12.
4. Fulghesu AM, Piras C, Dessì A, et al. Urinary Metabolites Reveal Hyperinsulinemia and Insulin Resistance in Polycystic Ovarian Syndrome (PCOS). Metabolites. 2021;11(7):437.
5. Dursun E, Akalın FA, Güncü GN, et al. Periodontal disease in polycystic ovary syndrome. Fertil. Steril. 2011;95(1):320-323.
6. Kassebaum N, Bernabé E, Dahiya M, Bhandari B, Murray C, Marcenes W. Global burden of severe periodontitis in 1990-2010: a systematic review and meta-regression. J. Dent. Res. 2014;93(11):1045-1053.
7. Cardoso EM, Reis C, Manzanares-Céspedes MC. Chronic periodontitis, inflammatory cytokines, and interrelationship with other chronic diseases. Postgrad Med. 2018;130(1):98-104.
8. Bowen W, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011;45(1):69-86.
9. Koo H, Falsetta M, Klein M. The exopolysaccharide matrix: a virulence determinant of cariogenic biofilm. J. Dent. Res. 2013;92(12):1065-1073.
10. Motallaei MN, Yazdanian M, Tebyanian H, et al. The Current Strategies in Controlling Oral Diseases by Herbal and Chemical Materials. Evid Based Complementary Altern Med. 2021;2021
11. Arce J-C, Smitz J. Exogenous hCG activity, but not endogenous LH activity, is positively associated with live birth rates in anovulatory infertility. Hum Fertil. 2011;14(3):192-199.
12. Solorzano CMB, Beller JP, Abshire MY, Collins JS, McCartney CR, Marshall JC. Neuroendocrine dysfunction in polycystic ovary syndrome. Steroids. 2012;77(4):332-337.
13. Tedjosasongko U, Ramadhaniati DM, Pradopo S. Streptococcus mutans detection on mother-child pairs using matrix-assisted laser desorption ionization–time of flight mass spectrometry and polymerase chain reaction. Dental Journal (Majalah Kedokteran Gigi). 2021;54(1):52-56.
14. Al-Blooshi SY, Latif MAA, Sabaneh NK, Mgaogao M, Hossain A. Development of a novel selective medium for culture of Gram-negative bacteria. BMC Res Notes. 2021;14(1):1-6.
15. Ethirajulu A, Alkasabera A, Onyali CB, et al. Insulin Resistance, Hyperandrogenism, and Its Associated Symptoms Are the Precipitating Factors for Depression in Women With Polycystic Ovarian Syndrome. Cureus. 2021;13(9)
16. Szczuko M, Kikut J, Szczuko U, et al. Nutrition strategy and life style in polycystic ovary syndrome—Narrative review. Nutrients. 2021;13(7):2452.
17. Isola G. Advances in biomarkers and diagnostics in periodontitis and oral diseases. Multidisciplinary Digital Publishing Institute; 2021.
18. Aiuchi H, Kitasako Y, Fukuda Y, Nakashima S, Burrow M, Tagami J. Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential. Aust. Dent. J.2008;53(2):167-171.
19. Radović K, Brković B, Roganović J, Ilić J, Milić Lemić A, Jovanović B. Salivary VEGF and post-extraction wound healing in type 2 diabetic immediate denture wearers. Acta Odontol. Scand.2021:1-6.
20. Zhang X, Wang M, Wang X, et al. Relationship between periodontitis and microangiopathy in type 2 diabetes mellitus: a meta‐analysis. J. Periodontal Res.2021; 56:1019–1027.
21. Malicka B, Kaczmarek U, Skośkiewicz-Malinowska K. Prevalence of xerostomia and the salivary flow rate in diabetic patients. Adv Clin Exp Med 2014;23(2):225-233.
22. Boyce HW, Bakheet MR. Sialorrhea: a review of a vexing, often unrecognized sign of oropharyngeal and esophageal disease. J. Clin. Gastroenterol 2005;39(2):89-97.
23. Saleh J, Figueiredo MAZ, Cherubini K, Salum FG. Salivary hypofunction: an update on aetiology, diagnosis and therapeutics. Arch. Oral Biol 2015;60(2):242-255.
24. da Mata ADSP, Amaral JPdAR, Thomson WM, et al. Patient‐related outcomes in Sjögren syndrome treated with stimulants of salivary secretion: Randomized clinical trial. Oral Dis. 2020;26(2):313-324.
25. Özcan E, Bulut İ, Berk S, Çanakçı CF. Astımlı hastalarda kısa ve uzun dönem inhaler kortikosteroid kullanımının oral ve periodontal sağlık üzerine etkileri. 2011; 13(2): 16-22.
26. Mumcu G, Ergun T, Inanc Na, et al. Oral health is impaired in Behcet's disease and is associated with disease severity. Rheumatology. 2004;43(8):1028-1033.
27. Loyola Rodriguez J, Galvan Torres L, Martinez Martinez R, et al. Frequency of dental caries in active and inactive systemic lupus erythematous patients: salivary and bacterial factors. Lupus. 2016;25(12):1349-1356.
28. Russell SL, Mayberry LJ. Pregnancy and oral health: a review and recommendations to reduce gaps in practice and research. MCN Am J Matern Child Nurs . 2008;33(1):32-37.
29. Rajesh K, Zareena SH, Kumar MA. Assessment of salivary calcium, phosphate, magnesium, pH, and flow rate in healthy subjects, periodontitis, and dental caries. Contemp. Clin. Dent." 2015;6(4):461.
30. Erdem V, Yıldız M, Erdem T. The evaluation of saliva flow rate, pH, buffer capacity, microbiological content and indice of decayed, missing and filled teeth in behçet’s patients. Balkan Med J. 2013;30(2):211.
31. Curtis MA, Diaz PI, Van Dyke TE. The role of the microbiota in periodontal disease. Periodontol 2000. 2020;83(1):14-25.
32. Nair SD, Varma S, Suragimath G, Zope S, Kale V, Abbayya K. Prevalence of periodontal disease in women with polycystic ovary syndrome-a comparative descriptive study. J Evol Med Dent Sci. 2017;6:4733-4736.
33. Porwal S, Tewari S, Sharma RK, Singhal SR, Narula SC. Periodontal status and high‐sensitivity C‐reactive protein levels in polycystic ovary syndrome with and without medical treatment. J. Periodontol.2014;85(10):1380-1389.
34. Surmelioglu D, Yeniceri E, İsman O, Gunduz R. Evaluation Of Saliva Analysis And Dmft Indexs In Polycystic Ovary Syndrome. Akd Med J 2020;6:346-350.
35. Siudikiene J, Machiulskiene V, Nyvad B, Tenovuo J, Nedzelskiene I. Dental caries and salivary status in children with type 1 diabetes mellitus, related to the metabolic control of the disease. Eur. J. Oral Sci. 2006;114(1):8-14.
36. Nanda J, Sachdev V, Sandhu M, Deep-Singh-Nanda K. Correlation between dental caries experience and mutans streptococci counts using saliva and plaque as microbial risk indicators in 3-8 year old children. A cross sectional study. J Clin Exp Dent 2015;7(1):e114.
37. Rodrigues MJ, Menezes VA, Marques KMG, Santos FAd. Caries prevalence and socioeconomic factors in children with sickle cell anemia. Braz. Oral Res 2012;26(1):43-49.
38. Erdem V, Yildiz M, Erdem T. The Evaluation of Saliva Flow Rate. Balkan Med J. 2013;30(2)
39. Glodny B, Nasseri P, Crismani A, et al. The occurrence of dental caries is associated with atherosclerosis. Clinics. 2013;68(7):946-953.
40. Silva AER, Menezes AMB, Demarco FF, Vargas-Ferreira F, Peres MA. Obesity and dental caries: systematic review. Rev Saude Publica 013;47:799-812.
41. Syrjälä A-MH, Niskanen MC, Ylöstalo P, Knuuttila ML. Metabolic control as a modifier of the association between salivary factors and dental caries among diabetic patients. Caries Res. 2003;37(2):142-147.
42. Nakahara Y, Sano T, Kodama Y, Ozaki K, Matsuura T. Alloxan-induced hyperglycemia causes rapid-onset and progressive dental caries and periodontitis in F344 rats. Histol Histopathol 2012; 27: 1297-1306. 43. Bowden G. The microbial ecology of dental caries. Microb Ecol Health Dis. 2000;12(3):138-148.

There are 42 citations in total.

Details

Primary Language	English
Subjects	Health Care Administration
Journal Section	Original Research Articles
Authors	N. Ezgi Yeniçeri Hilaloğlu 0000-0003-4415-4944 Derya Gursel Surmelioglu 0000-0002-6034-3131
Project Number	DHF.UT.19.04.
Publication Date	June 30, 2022
Submission Date	June 17, 2022
Published in Issue	Year 2022Volume: 25 Issue: 2

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EndNote	Yeniçeri Hilaloğlu NE, Gursel Surmelioglu D (June 1, 2022) ASSESSMENT OF DMFT INDEXES, SALIVARY FLOW RATE, PH, AND DETECTIONS OF S.MUTANS SALIVARY LEVELS BY A QUANTITATIVE REAL-TIME PCR IN POLYCYSTIC OVARY SYNDROME. Cumhuriyet Dental Journal 25 2 163–171.

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