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Year 2021, Volume: 24 Issue: 4, 361 - 370, 03.01.2022

Muhammet Şengül , Vildan Bostancı

https://doi.org/10.7126/cumudj.999234

Abstract

Supporting Institution

CUMHURİYET ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ

Project Number

DİŞ-257

References

  • REFERENCES 1. Giannoudis P, Psarakis S, Kontakis GJI. Can we accelerate fracture healing?: A critical analysis of the literature. 38(1), S81-S89, 2007.
  • 2. Gutierrez G, Lalka D, Garrett I, Rossini G, Mundy GJOi. Transdermal application of lovastatin to rats causes profound increases in bone formation and plasma concentrations. 17(7), 1033-42, 2006.
  • 3. Urist MRJS. Bone: formation by autoinduction. 150(3698), 893-99, 1965.
  • 4. Hollinger JO, Mark DE, Goco P, Quigley N, Desverreaux RW, Bach DEJCo ve ark. A comparison of four particulate bone derivatives. (267), 255-63, 1991.
  • 5. Senesi N, Miano TM, Provenzano MR, Brunetti G. Characterization, differentiation, and classification of humic substances by fluorescence spectroscopy. Soil Science, 152(4), 259-71, 1991.
  • 6. Davies G. Properties and Functions of Humic Acids. Meeting Report. The Nucleus Febr, 17, 1996.
  • 7. Orlov D. Humic substances in the biosphere. Soros Educ J, (2), 56-63, 1997.
  • 8. Klöcking R, Helbig B. Medical aspects and applications of humic substances. Biopolymers for Medical and Pharmaceutical Applications WILEY-VCH Verlag GmbH & C KGaA Weinheim, 3-16, 2005.
  • 9. Feldkamp LA, Goldstein SA, Parfitt MA, Jesion G, Kleerekoper M. The direct examination of three‐dimensional bone architecture in vitro by computed tomography. Journal of bone and mineral research, 4(1), 3-11, 1989.
  • 10. Kuhn J, Goldstein S, Feldkamp L, Goulet R, Jesson G. Evaluation of a microcomputed tomography system to study trabecular bone structure. Journal of Orthopaedic Research, 8(6), 833-42, 1990.
  • 11. Şahin FÜ, Topuz Ö. Diş hekimliği araştırmalarında mikrobilgisayarlı tomografi uygulamaları. Acta Odontologica Turcica, 31(2), 114-20, 2014.
  • 12. Gielkens PF, Schortinghuis J, de Jong JR, Huysmans MCD, van Leeuwen MBM, Raghoebar GM ve ark. A comparison of micro-CT, microradiography and histomorphometry in bone research. Archives of Oral Biology, 53(6), 558-66, 2008.
  • 13. Nazirkar G, Singh S, Dole V, Nikam A. Effortless effort in bone regeneration: a review. Journal of international oral health: JIOH, 6(3), 120, 2014.
  • 14. Zimmermann G, Moghaddam A. Allograft bone matrix versus synthetic bone graft substitutes. Injury, 42, S16-S21, 2011.
  • 15. Van Rensburg C, Snyman J, Mokoele T, Cromarty A. Brown coal-derived humate inhibits contact hypersensitivity; an efficacy, toxicity and teratogenicity study in rats. Inflammation, 30(5), 148-52, 2007.
  • 16. Feldkamp LA, Goldstein SA, Parfitt MA, Jesion G, Kleerekoper M. The direct examination of three‐dimensional bone architecture in vitro by computed tomography. Journal of bone and mineral research, 4(1), 3-11, 1989.
  • 17. Van Rensburg CEJ, Naude PJ. Potassium humate inhibits complement activation and the production of inflammatory cytokines in vitro. Inflammation, 32(4), 270-76, 2009.
  • 18. Gau R-J, Yang H-L, Chow S-N, Suen J-L, Lu F-J. Humic acid suppresses the LPS-induced expression of cell-surface adhesion proteins through the inhibition of NF-κB activation. Toxicology and applied pharmacology, 166(1), 59-67, 2000.
  • 19. Botes ME, Dekker J, van Rensburg C. Phase I trial with oral oxihumate in HIV‐infected patients. Drug development research, 57(1), 34-39, 2002.
  • 20. Kreutz B, Schlikekewey W. Effects of implanted bovine calcium hydroxyapatite with humate. Arch Orthop Trauma Surg, 111(5), 259-64, 1992.
  • 21. Frame J. A convenient animal model for testing bone substitute materials. Journal of oral surgery (American Dental Association: 1965), 38(3), 176-80, 1980.
  • 22. Prolo DJ, Gutierrez RV, DeVine JS, Okerlund SA. Clinical utility of allogeneic skull discs in human craniotomy. Neurosurgery, 14(2), 183-86, 1984.
  • 23. Özeç İ, Kılıç E, Gümüş C, Göze F. Lokal olarak üç farklı dozda simvastatin uygulamasının kemik defekti iyileşmesi üze rine etkisinin değerlendirilmesi. Cumhuriyet Üniversitesi Diş Hekimliği Fakültesi Dergisi, 10(2), 82-86, 2007.
  • 24. Donos N, Graziani F, Mardas N, Kostopoulos L. The use of human hypertrophic chondrocytes‐derived extracellular matrix for the treatment of critical‐size calvarial defects. Clinical oral implants research, 22(12), 1346-53, 2011.
  • 25. Develioğlu H. Kritik boyutlu ve kritik boyutlu olmayan defektler. Cumhuriyet Üniversitesi Diş Hek Fak Derg, 6(1), 60-63, 2003.
  • 26. Takagi K, Urist MR. The role of bone marrow in bone morphogenetic protein-induced repair of femoral massive diaphyseal defects. Clinical orthopaedics and related research, (171), 224-31, 1982.
  • 27. Mulliken JB, Glowacki J. Induced osteogenesis for repair and construction in the craniofacial region. Plastic and reconstructive surgery, 65(5), 553-60, 1980.
  • 28. Glowacki J, Altobelli D, Mulliken JB. Fate of mineralized and demineralized osseous implants in cranial defects. Calcified tissue international, 33(1), 71-76, 1981.
  • 29. Prolo DJ, Pedrotti PW, Burres KP, Okerlund S. Superior osteogenesis in transplanted allogeneic canine skull following chemical sterilization. Clinical orthopaedics and related research, (168), 230-42, 1982.
  • 30. OU T. Rifamisin ile dekontamine edilen otojen blok kemiğin onley greft olarak kullanımının deneysel olarak incelenmesi. [Doktora Tezi], Cumhuriyet Üniversitesi Sağlık Bilimleri Enstitüsü, 2011.
  • 31. Y. B. Nitrik oksidin kemik iyileşmesinde rolü. [Uzmanlık Tezi], İstanbul Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı. , 2000.
  • 32. Şahin FÜ, Topuz Ö. Diş hekimliği araştırmalarında mikrobilgisayarlı tomografi uygulamaları. Acta Odontologica Turcica, 31(2), 114-20, 2014.
  • 33. Gielkens PF, Schortinghuis J, de Jong JR, Huysmans MCD, van Leeuwen MBM, Raghoebar GM ve ark. A comparison of micro-CT, microradiography and histomorphometry in bone research. Archives of Oral Biology, 53(6), 558-66, 2008.
  • 34. Kumasaka A, Iikubo M, Nishioka T, Kojima I, Shoji N, Sakamoto M ve ark. Insulin‐Like Growth Factor I Inhibits Alveolar Bone Loss Following Tooth Extraction in Rats. Clinical Implant Dentistry and Related Research, 17(6), 1174-79, 2015.
  • 35. Histing T, Andonyan A, Klein M, Scheuer C, Stenger D, Holstein J ve ark. Obesity does not affect the healing of femur fractures in mice. Injury, 47(7), 1435-44, 2016.
  • 36. King S, Baptiston Tanaka C, Ross D, Kruzic JJ, Levinger I, Klineberg I ve ark. A diet high in fat and fructose adversely affects osseointegration of titanium implants in rats. Clinical and experimental dental research, 6(1), 107-16, 2020.
  • 37. Çalışır M, Akpınar A, Poyraz Ö, Göze F, Çınar Z. The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature‐induced periodontitis in rats. Journal of Periodontal Research, 51(4), 499-507, 2016.
  • 38. Tkachenko S, Rutskiĭ V, Grachev I. under the effect of mumie-asyl. Ortopediia Travmatologiia I Protezirovanie [Ortop Travmatol Profitez], 49-52, 1979.
  • 39. Kel'ginbaev N, Sorokina V, Stefani A, Ismailova V. Treatment of long tubular bone fractures with Mumie Assil preparations in experiments and clinical conditions. Eksperimental'naia khirurgiia i anesteziologiia, 18(4), 31-35, 1973.
  • 40. Durmuş K, Bora A, Doğan M, Özer H, Tuncer E, Altuntaş EE. Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates, 7(2), 57-61, 2017.

INVESTIGATION OF THE EFFECT OF HUMIC ACID ON BONE REGENERATION IN PARIETAL DEFECTS FORMED IN RATS RATLARDA OLUŞTURULAN PARİETAL DEFEKTLERDE HUMİK ASİTİN KEMİK REJENERASYONU ÜZERİNE ETKİSİNİN ARAŞTIRILMASI

Year 2021, Volume: 24 Issue: 4, 361 - 370, 03.01.2022

Muhammet Şengül , Vildan Bostancı

https://doi.org/10.7126/cumudj.999234

Abstract

INVESTIGATION OF THE EFFECT OF HUMIC ACID ON BONE REGENERATION IN PARIETAL DEFECTS FORMED IN RATS


ABSTRACT
Objectives: The objective of our study is to research the effect of the local humic acid on the premature new bone amount in autogenous bone graft applications.
Materials and Methods: 12-Week 24 rats of Wistar Albino species with a ranging weight of 230-250 g were used as subjects. The laboratory animals were divided into 4 groups. Those were determined as the control group, only autogenous graft-applied group, autogenous graft group applied with 100 mg/kg humic acid and autogenous graft group applied with 200 mg/kg humic acid. The bicortical bone fragment in the diameter of 5 mm at a critical size on the parietal bone of the laboratory animals was removed under physiological saline solution irrigation using an automax drill. The autogene graft obtained during the formation on the defected area was applied to the relevant experimental groups. The amount of humic acid determined for the groups of local application was applied by being mixed with the autogene graft. The laboratory animals were sacrificed on the 28th day following the procedure. Then the grafted defect area was evaluated using micro-computed tomography (Micro- CT).
Results: The highest value among trabecular thickness (Tb.Th) and the ratio of the bone volume to the tissue volume (BV/ TV) was encountered in the autogenous graft group applied with 100mg/kg humic acid. The highest value in the bone volume (BV) variance was detected in the group to which only the autogenous graft was applied. A statistically significant difference was found between the control group and the only autogenous graft-applied group and the autogenous graft group applied with local 100mg/kg humic acid upon comparing the groups in pairs for bone surface area (BS) variance. A statistically significant difference was found between the control group and the other groups in the ratio of bone surface are to the bone volume (BS/BV). BS/BV value was found higher in the autogenous graft group applied with local 200mg/kg humic acid than the autogenous group applied with local 100mg/kg humic acid.
Conclusions: The applied humic acid caused a decrease in the BV value. However, the positive effect of the humic acid application was observed in Tb.Th and BV/TV values, dosage increase negatively impacted the same. The dosage increase negatively affected the BS value. No positive effect of the humic acid application to BS/BV value was observed. The statistical significance between the control group and the other groups is considered to be autogenous graft.

Keywords

Humic acid autogenous bone graft bone regeneration

Project Number

DİŞ-257

References

  • REFERENCES 1. Giannoudis P, Psarakis S, Kontakis GJI. Can we accelerate fracture healing?: A critical analysis of the literature. 38(1), S81-S89, 2007.
  • 2. Gutierrez G, Lalka D, Garrett I, Rossini G, Mundy GJOi. Transdermal application of lovastatin to rats causes profound increases in bone formation and plasma concentrations. 17(7), 1033-42, 2006.
  • 3. Urist MRJS. Bone: formation by autoinduction. 150(3698), 893-99, 1965.
  • 4. Hollinger JO, Mark DE, Goco P, Quigley N, Desverreaux RW, Bach DEJCo ve ark. A comparison of four particulate bone derivatives. (267), 255-63, 1991.
  • 5. Senesi N, Miano TM, Provenzano MR, Brunetti G. Characterization, differentiation, and classification of humic substances by fluorescence spectroscopy. Soil Science, 152(4), 259-71, 1991.
  • 6. Davies G. Properties and Functions of Humic Acids. Meeting Report. The Nucleus Febr, 17, 1996.
  • 7. Orlov D. Humic substances in the biosphere. Soros Educ J, (2), 56-63, 1997.
  • 8. Klöcking R, Helbig B. Medical aspects and applications of humic substances. Biopolymers for Medical and Pharmaceutical Applications WILEY-VCH Verlag GmbH & C KGaA Weinheim, 3-16, 2005.
  • 9. Feldkamp LA, Goldstein SA, Parfitt MA, Jesion G, Kleerekoper M. The direct examination of three‐dimensional bone architecture in vitro by computed tomography. Journal of bone and mineral research, 4(1), 3-11, 1989.
  • 10. Kuhn J, Goldstein S, Feldkamp L, Goulet R, Jesson G. Evaluation of a microcomputed tomography system to study trabecular bone structure. Journal of Orthopaedic Research, 8(6), 833-42, 1990.
  • 11. Şahin FÜ, Topuz Ö. Diş hekimliği araştırmalarında mikrobilgisayarlı tomografi uygulamaları. Acta Odontologica Turcica, 31(2), 114-20, 2014.
  • 12. Gielkens PF, Schortinghuis J, de Jong JR, Huysmans MCD, van Leeuwen MBM, Raghoebar GM ve ark. A comparison of micro-CT, microradiography and histomorphometry in bone research. Archives of Oral Biology, 53(6), 558-66, 2008.
  • 13. Nazirkar G, Singh S, Dole V, Nikam A. Effortless effort in bone regeneration: a review. Journal of international oral health: JIOH, 6(3), 120, 2014.
  • 14. Zimmermann G, Moghaddam A. Allograft bone matrix versus synthetic bone graft substitutes. Injury, 42, S16-S21, 2011.
  • 15. Van Rensburg C, Snyman J, Mokoele T, Cromarty A. Brown coal-derived humate inhibits contact hypersensitivity; an efficacy, toxicity and teratogenicity study in rats. Inflammation, 30(5), 148-52, 2007.
  • 16. Feldkamp LA, Goldstein SA, Parfitt MA, Jesion G, Kleerekoper M. The direct examination of three‐dimensional bone architecture in vitro by computed tomography. Journal of bone and mineral research, 4(1), 3-11, 1989.
  • 17. Van Rensburg CEJ, Naude PJ. Potassium humate inhibits complement activation and the production of inflammatory cytokines in vitro. Inflammation, 32(4), 270-76, 2009.
  • 18. Gau R-J, Yang H-L, Chow S-N, Suen J-L, Lu F-J. Humic acid suppresses the LPS-induced expression of cell-surface adhesion proteins through the inhibition of NF-κB activation. Toxicology and applied pharmacology, 166(1), 59-67, 2000.
  • 19. Botes ME, Dekker J, van Rensburg C. Phase I trial with oral oxihumate in HIV‐infected patients. Drug development research, 57(1), 34-39, 2002.
  • 20. Kreutz B, Schlikekewey W. Effects of implanted bovine calcium hydroxyapatite with humate. Arch Orthop Trauma Surg, 111(5), 259-64, 1992.
  • 21. Frame J. A convenient animal model for testing bone substitute materials. Journal of oral surgery (American Dental Association: 1965), 38(3), 176-80, 1980.
  • 22. Prolo DJ, Gutierrez RV, DeVine JS, Okerlund SA. Clinical utility of allogeneic skull discs in human craniotomy. Neurosurgery, 14(2), 183-86, 1984.
  • 23. Özeç İ, Kılıç E, Gümüş C, Göze F. Lokal olarak üç farklı dozda simvastatin uygulamasının kemik defekti iyileşmesi üze rine etkisinin değerlendirilmesi. Cumhuriyet Üniversitesi Diş Hekimliği Fakültesi Dergisi, 10(2), 82-86, 2007.
  • 24. Donos N, Graziani F, Mardas N, Kostopoulos L. The use of human hypertrophic chondrocytes‐derived extracellular matrix for the treatment of critical‐size calvarial defects. Clinical oral implants research, 22(12), 1346-53, 2011.
  • 25. Develioğlu H. Kritik boyutlu ve kritik boyutlu olmayan defektler. Cumhuriyet Üniversitesi Diş Hek Fak Derg, 6(1), 60-63, 2003.
  • 26. Takagi K, Urist MR. The role of bone marrow in bone morphogenetic protein-induced repair of femoral massive diaphyseal defects. Clinical orthopaedics and related research, (171), 224-31, 1982.
  • 27. Mulliken JB, Glowacki J. Induced osteogenesis for repair and construction in the craniofacial region. Plastic and reconstructive surgery, 65(5), 553-60, 1980.
  • 28. Glowacki J, Altobelli D, Mulliken JB. Fate of mineralized and demineralized osseous implants in cranial defects. Calcified tissue international, 33(1), 71-76, 1981.
  • 29. Prolo DJ, Pedrotti PW, Burres KP, Okerlund S. Superior osteogenesis in transplanted allogeneic canine skull following chemical sterilization. Clinical orthopaedics and related research, (168), 230-42, 1982.
  • 30. OU T. Rifamisin ile dekontamine edilen otojen blok kemiğin onley greft olarak kullanımının deneysel olarak incelenmesi. [Doktora Tezi], Cumhuriyet Üniversitesi Sağlık Bilimleri Enstitüsü, 2011.
  • 31. Y. B. Nitrik oksidin kemik iyileşmesinde rolü. [Uzmanlık Tezi], İstanbul Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı. , 2000.
  • 32. Şahin FÜ, Topuz Ö. Diş hekimliği araştırmalarında mikrobilgisayarlı tomografi uygulamaları. Acta Odontologica Turcica, 31(2), 114-20, 2014.
  • 33. Gielkens PF, Schortinghuis J, de Jong JR, Huysmans MCD, van Leeuwen MBM, Raghoebar GM ve ark. A comparison of micro-CT, microradiography and histomorphometry in bone research. Archives of Oral Biology, 53(6), 558-66, 2008.
  • 34. Kumasaka A, Iikubo M, Nishioka T, Kojima I, Shoji N, Sakamoto M ve ark. Insulin‐Like Growth Factor I Inhibits Alveolar Bone Loss Following Tooth Extraction in Rats. Clinical Implant Dentistry and Related Research, 17(6), 1174-79, 2015.
  • 35. Histing T, Andonyan A, Klein M, Scheuer C, Stenger D, Holstein J ve ark. Obesity does not affect the healing of femur fractures in mice. Injury, 47(7), 1435-44, 2016.
  • 36. King S, Baptiston Tanaka C, Ross D, Kruzic JJ, Levinger I, Klineberg I ve ark. A diet high in fat and fructose adversely affects osseointegration of titanium implants in rats. Clinical and experimental dental research, 6(1), 107-16, 2020.
  • 37. Çalışır M, Akpınar A, Poyraz Ö, Göze F, Çınar Z. The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature‐induced periodontitis in rats. Journal of Periodontal Research, 51(4), 499-507, 2016.
  • 38. Tkachenko S, Rutskiĭ V, Grachev I. under the effect of mumie-asyl. Ortopediia Travmatologiia I Protezirovanie [Ortop Travmatol Profitez], 49-52, 1979.
  • 39. Kel'ginbaev N, Sorokina V, Stefani A, Ismailova V. Treatment of long tubular bone fractures with Mumie Assil preparations in experiments and clinical conditions. Eksperimental'naia khirurgiia i anesteziologiia, 18(4), 31-35, 1973.
  • 40. Durmuş K, Bora A, Doğan M, Özer H, Tuncer E, Altuntaş EE. Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates, 7(2), 57-61, 2017.
There are 40 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research Articles
Authors

Muhammet Şengül 0000-0002-6101-4647

Vildan Bostancı 0000-0002-4460-301X

Project Number DİŞ-257
Publication Date January 3, 2022
Submission Date September 24, 2021
Published in Issue Year 2021Volume: 24 Issue: 4

Cite

EndNote Şengül M, Bostancı V (January 1, 2022) INVESTIGATION OF THE EFFECT OF HUMIC ACID ON BONE REGENERATION IN PARIETAL DEFECTS FORMED IN RATS RATLARDA OLUŞTURULAN PARİETAL DEFEKTLERDE HUMİK ASİTİN KEMİK REJENERASYONU ÜZERİNE ETKİSİNİN ARAŞTIRILMASI. Cumhuriyet Dental Journal 24 4 361–370.
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