Research Article
BibTex RIS Cite

Year 2020, Volume: 23 Issue: 4, 371 - 381, 31.12.2020

Sevim Atılan Yavuz Derya Surmelioglu

https://doi.org/10.7126/cumudj.792699
Cited By: 2

Abstract

References

  • 1. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clinical Oral Investig. 1998;1:154-162
  • 2. Perdigão J. Dentin bonding—Variables related to the clinical situation and the substrate treatment. Dent Mater .2010; 26:24-37
  • 3. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems: I: Depth of penetration beyond dentin smear layers. Dent Mater. 2001; 17:296-308
  • 4. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeck B, De Munck. Bonding effectiveness of a new ‘multi-mode’adhesive to enamel and dentine. J Dent. 2012;40:475-484
  • 5. Perdigão J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multi-purpose dentin adhesive. Am J Dent. 2012;25:153
  • 6. Muñoz MA, Luque I, Hass V, Reis A, Loguercio AD, Bombarda NHC. Immediate bonding properties of universal adhesives to dentine. J Dent. 2013;41:404-411.
  • 7. Lawson NC, Robles A, Fu CC, Lin CP, Sawlani K, Burgess JO. Two-year clinical trial of a universal adhesive in total-etch and self-etch mode in non-carious cervical lesions. J Dent. 2015;43:1229-1234.
  • 8. Al‐Dawood A, Wennberg A. Biocompatibility of dentin bonding agents. Denta Traumatol. 1993;9:1-7.
  • 9. Kusdemir M, Gunal S, Ozer F, Imazato S, Izutani N, Ebisu S, Blatz MB. Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests. Dent Mater J. 2011;799.
  • 10. Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials. 2009, Springer 1.
  • 11. Lanza CRM, Costa CAS, Furlan M, Alecio A, Hebling J. Transdentinal diffusion and cytotoxicity of self-etching adhesive systems. Cell Biol Toxicol 2009;25:533.
  • 12. Schedle A, Franz A, Raush-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dental Mater. 1998;14:429-440.
  • 13. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit Rev Oral Biol Med. 200;11:333-355.
  • 14. Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982; 16:265-273. 15. Schmalz G. Materials science: biological aspects. J Dent Res. 2002;81:660
  • 16. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602-1606.
  • 17. Tay FR, Carvalho R, Sado H, Pashley DH.Effect of smear layers on the bonding of a self-etching primer to dentin. J Adhes Dent. 200;2:99-116.
  • 18. Bergenholtz G. Evidence for bacterial causation of adverse pulpal responses in resin-based dental restorations. Crit Rev Oral Biol Med. 200;11:467-480. 19. Chen, RS, Liu CC, Tseng WY, Jeng JH, Lin CP. Cytotoxicity of three dentin bonding agents on human dental pulp cells. J Dent. 2003;31:223-229.
  • 20. Hashieh IA, Cosset A, Franquin JC, Camps J. In vitro cytotoxicity of one-step dentin bonding systems. J Endod. 1999;25:89-92.
  • 21. Karapınar‐Kazandag M, Bayrak ÖF, Yalvaç ME, Ersev H, Tanalp J, Şahin F, Bayırlı G. Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental pulp cells. Int Endod J. 2011; 44:626-634.
  • 22. Yasuda Y, Inuyama H, Maeda H, Akamine A, Nör JE, Saito T. Cytotoxicity of one‐step dentin‐bonding agents toward dental pulp and odontoblast‐like cells. J Oral Rehabil. 2008;35:940-946.
  • 23. Murray PE, About I, Lumley PJ, Franquin JC, remusat M, Smith AJ. Cavity remaining dentin thickness and pulpal activity. Am J Dent. 2012;15:41-46.
  • 24. Albuquerque M, Pegoraro M, Mattei G, Reis A, Loguercio AD. Effect of double-application or the application of a hydrophobic layer for improved efficacy y of one-step self-etch systems in enamel and dentin. Oper Dent. 2008;33:564-570.
  • 25. Perdigão J, Munoz MA, sezinando A, Luquel-Martinez IV, Staichak R, reis A, Loguerco AD. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper Dent. 2014;39:489-499.
  • 26. Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J Biomed Mater Res B. 2008;86:523-529.
  • 27. Chen C, Niu LN, Xie H, Zhang ZY, Zhou LQ, Jiao K, Chen JH, Pashley DH, Tay FR. Bonding of universal adhesives to dentine–Old wine in new bottles? J Dent. 2015; 43:525-536.
  • 28. Ergün G, Eğilmez F, Üçtaşlı MB, Yilmaz Ş. Effect of light curing type on cytotoxicity of dentine‐bonding agents. Int Endod J. 2006;40:216-223.
  • 29. Kılıç K, Kesim B, Sümer Z, Polat Z, Kesim S. Evalution of Biocompatibility of Full Ceramics with MTT. J Health Sci. 2010;19:125-132.
  • 30. da Silva JM, Rodrigues JR, Camargo CHS, Fernandes VVB, Hiller KA, Schweiki H, Schmalz G. Effectiveness and biological compatibility of different generations of dentin adhesives. Clin Oral Investig. 2014;18:607-613.
  • 31. Wataha J, Rueggeberg FA, Lapp CA, Lewis CA, Lockwood PE, Ergle JW, Mettenburg DJ. In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva. Clin Oral Investig. 1999;3:144-149.
  • 32. Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater. 2002;18:318-323.
  • 33. Bouillaguet S, Shaw L, Gonzalez L, Wataha JC, Krejci L. Long‐term cytotoxicity of resin‐based dental restorative materials. J Oral Rehabil. 2002;29:7-13.
  • 34. Wataha JC, Craig RG, Hanks C. Precision of and new methods for testing in vitro alloy cytotoxicity. Dent Mater. 1992;8:65-70.
  • 35. Ke N, Wang X, Xu X, Abassi YA. The xCELLigence system for real-time and label-free monitoring of cell viability, in Mammalian Cell Viability. Mammalian Cell Viability. 2011;740:33-43.
  • 36. Urcan E, Haertel U, Styllou M, Hickel R, Scherthan H, Reichl FX. Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts. Dent Mater.201;26:51-58.
  • 37. Malkoç S, Öztürk F, Çörekçi B, bozkurt BS, Hakki SS. Real-time cell analysis of the cytotoxicity of orthodontic mini-implants on human gingival fibroblasts and mouse osteoblasts. Am J Orthod Dentofac. 2012;141:419-426.
  • 38. Golke A, Cymerys j, Stonska A, Dzieciakowski T, Chmielewska a, Tucholska A,Banbura MW. The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection. Pol J Vet Sci. 2012;15:151-153.
  • 39. Ruyter I. Physical and chemical aspects related to substances released from polymer materials in an aqueous environment. Adv Dent Res. 1995;9:344-347.
  • 40. Geurtsen W, Spahl W, Leyhausen G. Variability of cytotoxicity and leaching of substances from four light‐curing pit and fissure sealants. J Biomed Mater Res. 1999; 44:73-77.
  • 41. Gerzina T, Hume W. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent. 1996;24:125-128.
  • 42. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res. 1998; 41:474-480.
  • 43. Knezevic A, Zeljezic D, Kopjar N, Tarle Z. Cytotoxicity of composite materials polymerized with LED curing units. Oper Dent. 2008;33:23-30.
  • 44. Taira M, Nakao H, Matsumoto T, Takahashi J. Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts. Int J Prosthodont. 2000;13:311-315.
  • 45. Koulaouzidou EA, Papazisis KT, Yiannaki E, Palaghias G, Antoniades MH. Effects of dentin bonding agents on the cell cycle of fibroblasts. J Endod.2009;35:275-279.
  • 46. Rakich DR, Wataha JC, Lefebvre CA, Weller RN. Effects of dentin bonding agents on macrophage mitochondrial activity. J Endod 24(8):528-533.
  • 47. Al‐Hiyasat AS, Darmani H, Elbetieha AM. Leached components from dental composites and their effects on fertility of female mice. Eur J Oral Sci. 2009;112:267-272.
  • 48. Franz A, König F, Lucas T, Watts DC, Schedle A. Cytotoxic effects of dental bonding substances as a function of degree of conversion. Dent Mater. 2009;25:232-239.
  • 49. Schmalz G, Schuster U, Koch A, Schwiki H. Cytotoxicity of low pH dentin-bonding agents in a dentin barrier test in vitro. J Endod. 2002; 28:188-192.

EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM

Year 2020, Volume: 23 Issue: 4, 371 - 381, 31.12.2020

Sevim Atılan Yavuz Derya Surmelioglu

https://doi.org/10.7126/cumudj.792699
Cited By: 2

Abstract

ABSTRACT
Objectives: The purpose of this study was to explore the cytotoxic effects of five different universal bonding agents on mouse fibroblast cell lines (L929).
Materials and Methods: Five different widely used universal adhesive systems were chosen that have different contents, pH levels, and polymerization methods. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Germany, and ACEA Biosciences, USA) was used for cytotoxic evaluation of light-cured polymerized G-Premio Bond (GC Europe, Belgium), Prime&Bond Universal (Dentsply Sirona, USA), Universal Bond Quick (Kuraray, USA), Single Bond Universal (3M ESPE, USA) and self-cured polymerized Tokuyama Universal Bond (Tokuyama, USA) experimental groups. L929 were cultured in Dulbecco’s modified Eagle’s medium and supplemented with 10% fetal bovine serum and 1 % antibiotics. The assay was performed E-plate-16 and monitored every 15 min for 72 h. Statistical analysis was performed using ANOVA and Tukey’s posthoc tests.
Results: All tested universal adhesive systems showed a statistically significant difference in cytotoxicity values in different time periods (p<0.05). Among the groups compared, G-Premio Bond showed the least cytotoxic effect; and Tokuyama Universal Bond showed the most cytotoxic effect. Different times of all universal adhesive systems significantly increased the count of viable cells compared to the control group (p<0.05).
Conclusions: In dentistry, universal adhesive systems can be observed cytotoxic effects to live cells. The evaluation of cytotoxicity with xCELLigence device is a reliable method and should be supported by new studies on this subject.

Keywords

xCELLigence cytotoxicity universal adhesive systems L929

References

  • 1. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clinical Oral Investig. 1998;1:154-162
  • 2. Perdigão J. Dentin bonding—Variables related to the clinical situation and the substrate treatment. Dent Mater .2010; 26:24-37
  • 3. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems: I: Depth of penetration beyond dentin smear layers. Dent Mater. 2001; 17:296-308
  • 4. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeck B, De Munck. Bonding effectiveness of a new ‘multi-mode’adhesive to enamel and dentine. J Dent. 2012;40:475-484
  • 5. Perdigão J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multi-purpose dentin adhesive. Am J Dent. 2012;25:153
  • 6. Muñoz MA, Luque I, Hass V, Reis A, Loguercio AD, Bombarda NHC. Immediate bonding properties of universal adhesives to dentine. J Dent. 2013;41:404-411.
  • 7. Lawson NC, Robles A, Fu CC, Lin CP, Sawlani K, Burgess JO. Two-year clinical trial of a universal adhesive in total-etch and self-etch mode in non-carious cervical lesions. J Dent. 2015;43:1229-1234.
  • 8. Al‐Dawood A, Wennberg A. Biocompatibility of dentin bonding agents. Denta Traumatol. 1993;9:1-7.
  • 9. Kusdemir M, Gunal S, Ozer F, Imazato S, Izutani N, Ebisu S, Blatz MB. Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests. Dent Mater J. 2011;799.
  • 10. Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials. 2009, Springer 1.
  • 11. Lanza CRM, Costa CAS, Furlan M, Alecio A, Hebling J. Transdentinal diffusion and cytotoxicity of self-etching adhesive systems. Cell Biol Toxicol 2009;25:533.
  • 12. Schedle A, Franz A, Raush-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dental Mater. 1998;14:429-440.
  • 13. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit Rev Oral Biol Med. 200;11:333-355.
  • 14. Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982; 16:265-273. 15. Schmalz G. Materials science: biological aspects. J Dent Res. 2002;81:660
  • 16. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602-1606.
  • 17. Tay FR, Carvalho R, Sado H, Pashley DH.Effect of smear layers on the bonding of a self-etching primer to dentin. J Adhes Dent. 200;2:99-116.
  • 18. Bergenholtz G. Evidence for bacterial causation of adverse pulpal responses in resin-based dental restorations. Crit Rev Oral Biol Med. 200;11:467-480. 19. Chen, RS, Liu CC, Tseng WY, Jeng JH, Lin CP. Cytotoxicity of three dentin bonding agents on human dental pulp cells. J Dent. 2003;31:223-229.
  • 20. Hashieh IA, Cosset A, Franquin JC, Camps J. In vitro cytotoxicity of one-step dentin bonding systems. J Endod. 1999;25:89-92.
  • 21. Karapınar‐Kazandag M, Bayrak ÖF, Yalvaç ME, Ersev H, Tanalp J, Şahin F, Bayırlı G. Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental pulp cells. Int Endod J. 2011; 44:626-634.
  • 22. Yasuda Y, Inuyama H, Maeda H, Akamine A, Nör JE, Saito T. Cytotoxicity of one‐step dentin‐bonding agents toward dental pulp and odontoblast‐like cells. J Oral Rehabil. 2008;35:940-946.
  • 23. Murray PE, About I, Lumley PJ, Franquin JC, remusat M, Smith AJ. Cavity remaining dentin thickness and pulpal activity. Am J Dent. 2012;15:41-46.
  • 24. Albuquerque M, Pegoraro M, Mattei G, Reis A, Loguercio AD. Effect of double-application or the application of a hydrophobic layer for improved efficacy y of one-step self-etch systems in enamel and dentin. Oper Dent. 2008;33:564-570.
  • 25. Perdigão J, Munoz MA, sezinando A, Luquel-Martinez IV, Staichak R, reis A, Loguerco AD. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper Dent. 2014;39:489-499.
  • 26. Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J Biomed Mater Res B. 2008;86:523-529.
  • 27. Chen C, Niu LN, Xie H, Zhang ZY, Zhou LQ, Jiao K, Chen JH, Pashley DH, Tay FR. Bonding of universal adhesives to dentine–Old wine in new bottles? J Dent. 2015; 43:525-536.
  • 28. Ergün G, Eğilmez F, Üçtaşlı MB, Yilmaz Ş. Effect of light curing type on cytotoxicity of dentine‐bonding agents. Int Endod J. 2006;40:216-223.
  • 29. Kılıç K, Kesim B, Sümer Z, Polat Z, Kesim S. Evalution of Biocompatibility of Full Ceramics with MTT. J Health Sci. 2010;19:125-132.
  • 30. da Silva JM, Rodrigues JR, Camargo CHS, Fernandes VVB, Hiller KA, Schweiki H, Schmalz G. Effectiveness and biological compatibility of different generations of dentin adhesives. Clin Oral Investig. 2014;18:607-613.
  • 31. Wataha J, Rueggeberg FA, Lapp CA, Lewis CA, Lockwood PE, Ergle JW, Mettenburg DJ. In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva. Clin Oral Investig. 1999;3:144-149.
  • 32. Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater. 2002;18:318-323.
  • 33. Bouillaguet S, Shaw L, Gonzalez L, Wataha JC, Krejci L. Long‐term cytotoxicity of resin‐based dental restorative materials. J Oral Rehabil. 2002;29:7-13.
  • 34. Wataha JC, Craig RG, Hanks C. Precision of and new methods for testing in vitro alloy cytotoxicity. Dent Mater. 1992;8:65-70.
  • 35. Ke N, Wang X, Xu X, Abassi YA. The xCELLigence system for real-time and label-free monitoring of cell viability, in Mammalian Cell Viability. Mammalian Cell Viability. 2011;740:33-43.
  • 36. Urcan E, Haertel U, Styllou M, Hickel R, Scherthan H, Reichl FX. Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts. Dent Mater.201;26:51-58.
  • 37. Malkoç S, Öztürk F, Çörekçi B, bozkurt BS, Hakki SS. Real-time cell analysis of the cytotoxicity of orthodontic mini-implants on human gingival fibroblasts and mouse osteoblasts. Am J Orthod Dentofac. 2012;141:419-426.
  • 38. Golke A, Cymerys j, Stonska A, Dzieciakowski T, Chmielewska a, Tucholska A,Banbura MW. The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection. Pol J Vet Sci. 2012;15:151-153.
  • 39. Ruyter I. Physical and chemical aspects related to substances released from polymer materials in an aqueous environment. Adv Dent Res. 1995;9:344-347.
  • 40. Geurtsen W, Spahl W, Leyhausen G. Variability of cytotoxicity and leaching of substances from four light‐curing pit and fissure sealants. J Biomed Mater Res. 1999; 44:73-77.
  • 41. Gerzina T, Hume W. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent. 1996;24:125-128.
  • 42. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res. 1998; 41:474-480.
  • 43. Knezevic A, Zeljezic D, Kopjar N, Tarle Z. Cytotoxicity of composite materials polymerized with LED curing units. Oper Dent. 2008;33:23-30.
  • 44. Taira M, Nakao H, Matsumoto T, Takahashi J. Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts. Int J Prosthodont. 2000;13:311-315.
  • 45. Koulaouzidou EA, Papazisis KT, Yiannaki E, Palaghias G, Antoniades MH. Effects of dentin bonding agents on the cell cycle of fibroblasts. J Endod.2009;35:275-279.
  • 46. Rakich DR, Wataha JC, Lefebvre CA, Weller RN. Effects of dentin bonding agents on macrophage mitochondrial activity. J Endod 24(8):528-533.
  • 47. Al‐Hiyasat AS, Darmani H, Elbetieha AM. Leached components from dental composites and their effects on fertility of female mice. Eur J Oral Sci. 2009;112:267-272.
  • 48. Franz A, König F, Lucas T, Watts DC, Schedle A. Cytotoxic effects of dental bonding substances as a function of degree of conversion. Dent Mater. 2009;25:232-239.
  • 49. Schmalz G, Schuster U, Koch A, Schwiki H. Cytotoxicity of low pH dentin-bonding agents in a dentin barrier test in vitro. J Endod. 2002; 28:188-192.
There are 47 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research Articles
Authors

Sevim Atılan Yavuz 0000-0002-6192-4931

Derya Surmelioglu 0000-0002-6034-3131

Publication Date December 31, 2020
Submission Date September 9, 2020
Published in Issue Year 2020Volume: 23 Issue: 4

Cite

EndNote Atılan Yavuz S, Surmelioglu D (December 1, 2020) EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM. Cumhuriyet Dental Journal 23 4 371–381.

Cited By

An in vitro assessment of teething gels’ effects on human gingival mesenchymal stem cells
BMC Oral Health
https://doi.org/10.1186/s12903-024-04213-w
Evaluation of Biocompatibility Properties of Dental Materials: xCELLigence® System
ADO Klinik Bilimler Dergisi
https://doi.org/10.54617/adoklinikbilimler.1401043
 Download Cover Image

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


CDJ accepts articles in English. Submitting a paper to CDJ is free of charges. In addition, CDJ has not have article processing charges.

Frequency: Four times a year (March, June, September, and December)

IMPORTANT NOTICE

All users of Cumhuriyet Dental Journal should visit to their user's home page through the "https://dergipark.org.tr/tr/user" " or "https://dergipark.org.tr/en/user" links to update their incomplete information shown in blue or yellow warnings and update their e-mail addresses and information to the DergiPark system. Otherwise, the e-mails from the journal will not be seen or fall into the SPAM folder. Please fill in all missing part in the relevant field.

Please visit journal's AUTHOR GUIDELINE to see revised policy and submission rules to be held since 2020.