A STUDY ON THE USE OF DIFFERENT MATERIALS COMBINED WITH ALLOGRAFT ON OSSEOINTEGRATION AND BONE REGENERATION OF DENTAL IMPLANTS WITH CORONAL DEFECTS IN A RABBIT MODEL
Abstract
Objectives: To assess differential effects of different materials combined with allograft on bone-to-implant contact and newly formed bone formation in dental implants with coronal defects histomorphometrically.
Materials and Methods: The study was conducted on 24 male New Zealand white rabbits. Dental implants (3.0 × 10 mm) were placed at the center of defects (9 mm diameter, 4 mm depth) created in the tibial bones of the rabbits. Graft (GF, n=8), graft + rifamycin (GR, n=8), and graft + black cumin oil via orogastric route (GB, n=8) were applied on the coronal aspects of the implants for 28 days. Undecalcified histomorphometric analyses were conducted on slides stained with toluidine blue.
Results: Bone-to-implant contact was 46.57% ± 3.59% in the graft, 67.12% ± 3.64% in the graft+rifamycin, and 55.62% ± 4.37% in the graft+black cumin oil groups. The percentage of new bone formation at the defect area was 34.71% ± 4.11% in the graft, 55.37% ± 4.89% in the graft + rifamycin, and 45.75% ± 3.69% in the graft + black cumin oil groups. In terms of new bone formation and bone-to-implant contact, graft + rifamycin and graft + black cumin oil groups were significantly different from the graft group. The differences between the graft + rifamycin and graft + black cumin oil groups were also statistically significant.
Conclusion: Allogreft + rifamycin and orogastric black cumin oil were found to have positive effects on bone healing at sites with coronal defects. Rifamycin showed significantly greater favorable effects on bone-to-implant contact and new bone formation compared to black cumin oil.
Supporting Institution
Scientific Research Project Fund of Sivas Cumhuriyet University (CÜBAP)
Project Number
DIS-158
Thanks
The authors thank Dr. Erdem KILIÇ from the Erciyes University Oral and Maxillofacial Surgery Department for his support in the research laboratory work.
References
- 1. Misch CE, Qu Z, Bidez MW. Mechanical properties of trabecular bone in the human mandible: implications for dental implant treatment planning and surgical placement. J Oral Maxillofac Surg 1999;57(6): 700-706
- 2. Wood MR, Vermilyea SG. A review of selected dental literature on evidence-based treatment planning for dental implants: report of the Committee on Research in Fixed Prosthodontics of the Academy of Fixed Prosthodontics. J Prosthet Dent 2004;92(5): 447-462
- 3. Branemark PI, Hansson BO, Adell R, Breine U, Lindstrom J, Hallen O, Ohman A: Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period. Scand J Plast and Reconstr Surg Suppl 1977;16: 1-132
- 4. Alfaro FH. Bone Grafting in Oral Implantology Techniques and Clinical Applications. London: Quintessence 2006;9–15
- 5. Ezirganlı, Ş, Polat S, Barış E, Tatar İ, Çelik HH. Comparative investigation of the effects of different materials used with a titanium barrier on new bone formation. Clin Oral Implants Res 2013;24(3):312-319
- 6. Bowers GM, Chadroff B, Carnevale R, Mellonig J, Corio R, Emerson J, Stevens M, Romberg E. Histologic evaluation of new attachment apparatus formation in humans. J Periodontol 1989;60:664–674
- 7. Glowacki J, Altobelli D, Mulliken JB. Fate of mineralized and demineralized osseous implants in cranial defects. Calcif Tissue Int 1981;33(1):71-76
- 8. Simsek S, Ozec I, Kurkcu M, Benlidayı E. Histomorphometric Evaluation of Bone Formation in Peri-Implant Defects Treated With Different Regeneration Techniques: An Experimental Study in a Rabbit Model. J Oral Maxillofac Surg 2016;74: 1757-1764
- 9. Sisti F, Vaccaro L. Local application of rifamycin SV in caverns treated by endocavitary aspiration. Arch Tisiol Mal Appar Respir 1963;18:251-256
- 10. de Carvalho PS, Mariano RC, Okamoto T. Treatment of fibrinolytic alveolitis with rifamycin B diethylamide associated with gelfoam: a histological study. Braz Dent J 1997;8(1):3-8
- 11. Tasdemir U, Ozec I, Esen HH, Avunduk MC. The influence of rifamycin decontamination on incorporation of autologous onlay bone grafts in rats, a histometric and immunohistochemical evaluation. Arch Oral Biol 2015;60(5):724-729
- 12. Houghton PJ, Zarka R, De Las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived black cumin oil inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995;61(1):33-36
- 13. Ali BH, Blunden G. Pharmacological and toxicological properties of nigella sativa. Phytother res 2003;17(4):299-305
- 14. Al-Naggar TB, Gomez-Serranıllas MP, Carretero ME, Vıllar AM. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol 2003;88(1):63-68
- 15. Ku SK, Cho HR, Sung YS, Kang SJ, Lee YJ. Effects of calcium gluconate on experimental periodontitis and alveolar bone in rats. Basic Clin Pharmacol Toxicol 2011;108(4):241–250
- 16. Ozdemir H, Kara MI, Erciyas K, Ozer H, Ay S. Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. J Periodontal Res 2012;47(1):74–80
- 17. Weigl P. Implant prosthodontics: What next? Berlin: Quintessence Int 2003;34(9):653-669
- 18. Lachmann S, Laval JY, Jager B, Axmann D, Gomez-Roman G, Groten M, Weber H. Resonance frequency analysis and damping capacity assessment. Part 2: peri-implant bone loss follow-up. An in vitro study with the Periotest and Osstell instruments. Clin Oral Implants Res 2006;17(1):80-84
- 19. Chen ST, Darby IB, Reynolds EC. A prospective clinical study of non-submerged immediate implants: clinical outcomes and esthetic results. Clin Oral Implants Res 2007;18(5):552-562
- 20. McAllister BS, Margolin MD, Cogan AG, Taylor M, Wollins J. Residual lateral wall defects following sinus grafting with recombinant human osteogenic protein-1 or Bio-Oss in the chimpanzee. Int J Periodontics Restorative Dent 1998;18(3):227-239
- 21. Ozec I, Kilic E, Gumus C, Goze F. Effect of local simvastatin application on mandibular defects. J Craniofac Surg 2007;18(3):546-550
- 22. Takagi K, Urist MR. The reaction of the dura to bone morphogenetic protein (BMP) in repair of skull defects. Ann Surg 1982;196(1):100-109
- 23. Al-Sulaimani AF, Mokeem SA, Anil S. Peri-implant defect augmentation with autogenous bone: A study in beagle dogs. J Oral Implantol 2013;39(1):30-36
- 24. Schuler RF, Janakievski J, Hacker BM. Effect of implant surface and grafting on implants placed into simulated extraction sockets: A histologic study in dogs. Int J Oral Maxillofac Implants 2010;25(5):893-900
- 25. Witso E, Persen L, Loseth K, Benum P, Bergh K. Cancellous bone as an antibiotic carrier. Acta Orthop Scand 2000;71(1):80-84
- 26. Kaya A, Kaya B, Aktas¸ A, Fırat ET. Effect of rifampin in combination with allogeneic, alloplastic, and heterogenous bone grafts on bone regeneration in rat tibial bone defects. J Oral Maxillofac Surg Med Pathol 2015;27(1):20-28
- 27. Rathbone CR, Cross JD, Brown KV. Effect of various concentrations of antibiotics on osteogenic cell viability and activity. J Orthop Res 2011;29(7):1070-1074
- 28. Zhang Z, Wang X, Luo F. Effects of rifampicin on osteogenic differentiation and proliferation of human mesenchymal stem cells in the bone marrow. Genet Mol Res 2014;13(3):6398-6410
- 29. Isefuku S, Joyner CJ, Simpson AH: Toxic effect of rifampicin on human osteoblast-like cells. J Orthop Res 2001;19(5):950-954
- 30. Wirries A, Schubert AK, Zimmermann R, Jabari S, Ruchholtz S, El-Najjar N. Black cumin oil accelerates osteoblast differentiation and activates bone morphogenetic protein-2 and ERK pathway. Int Immunopharmacol 2013;15(2):381-386
- 31. Kara MI, Erciyas K, Altan AB, Ozkut M, Ay S, Inan S. Thymoquinone accelerates new bone formation in the rapid maxillary expansion procedure. Arch Oral Biol. 2012;57(4):357-363
Abstract
Project Number
DIS-158
References
- 1. Misch CE, Qu Z, Bidez MW. Mechanical properties of trabecular bone in the human mandible: implications for dental implant treatment planning and surgical placement. J Oral Maxillofac Surg 1999;57(6): 700-706
- 2. Wood MR, Vermilyea SG. A review of selected dental literature on evidence-based treatment planning for dental implants: report of the Committee on Research in Fixed Prosthodontics of the Academy of Fixed Prosthodontics. J Prosthet Dent 2004;92(5): 447-462
- 3. Branemark PI, Hansson BO, Adell R, Breine U, Lindstrom J, Hallen O, Ohman A: Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period. Scand J Plast and Reconstr Surg Suppl 1977;16: 1-132
- 4. Alfaro FH. Bone Grafting in Oral Implantology Techniques and Clinical Applications. London: Quintessence 2006;9–15
- 5. Ezirganlı, Ş, Polat S, Barış E, Tatar İ, Çelik HH. Comparative investigation of the effects of different materials used with a titanium barrier on new bone formation. Clin Oral Implants Res 2013;24(3):312-319
- 6. Bowers GM, Chadroff B, Carnevale R, Mellonig J, Corio R, Emerson J, Stevens M, Romberg E. Histologic evaluation of new attachment apparatus formation in humans. J Periodontol 1989;60:664–674
- 7. Glowacki J, Altobelli D, Mulliken JB. Fate of mineralized and demineralized osseous implants in cranial defects. Calcif Tissue Int 1981;33(1):71-76
- 8. Simsek S, Ozec I, Kurkcu M, Benlidayı E. Histomorphometric Evaluation of Bone Formation in Peri-Implant Defects Treated With Different Regeneration Techniques: An Experimental Study in a Rabbit Model. J Oral Maxillofac Surg 2016;74: 1757-1764
- 9. Sisti F, Vaccaro L. Local application of rifamycin SV in caverns treated by endocavitary aspiration. Arch Tisiol Mal Appar Respir 1963;18:251-256
- 10. de Carvalho PS, Mariano RC, Okamoto T. Treatment of fibrinolytic alveolitis with rifamycin B diethylamide associated with gelfoam: a histological study. Braz Dent J 1997;8(1):3-8
- 11. Tasdemir U, Ozec I, Esen HH, Avunduk MC. The influence of rifamycin decontamination on incorporation of autologous onlay bone grafts in rats, a histometric and immunohistochemical evaluation. Arch Oral Biol 2015;60(5):724-729
- 12. Houghton PJ, Zarka R, De Las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived black cumin oil inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995;61(1):33-36
- 13. Ali BH, Blunden G. Pharmacological and toxicological properties of nigella sativa. Phytother res 2003;17(4):299-305
- 14. Al-Naggar TB, Gomez-Serranıllas MP, Carretero ME, Vıllar AM. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol 2003;88(1):63-68
- 15. Ku SK, Cho HR, Sung YS, Kang SJ, Lee YJ. Effects of calcium gluconate on experimental periodontitis and alveolar bone in rats. Basic Clin Pharmacol Toxicol 2011;108(4):241–250
- 16. Ozdemir H, Kara MI, Erciyas K, Ozer H, Ay S. Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. J Periodontal Res 2012;47(1):74–80
- 17. Weigl P. Implant prosthodontics: What next? Berlin: Quintessence Int 2003;34(9):653-669
- 18. Lachmann S, Laval JY, Jager B, Axmann D, Gomez-Roman G, Groten M, Weber H. Resonance frequency analysis and damping capacity assessment. Part 2: peri-implant bone loss follow-up. An in vitro study with the Periotest and Osstell instruments. Clin Oral Implants Res 2006;17(1):80-84
- 19. Chen ST, Darby IB, Reynolds EC. A prospective clinical study of non-submerged immediate implants: clinical outcomes and esthetic results. Clin Oral Implants Res 2007;18(5):552-562
- 20. McAllister BS, Margolin MD, Cogan AG, Taylor M, Wollins J. Residual lateral wall defects following sinus grafting with recombinant human osteogenic protein-1 or Bio-Oss in the chimpanzee. Int J Periodontics Restorative Dent 1998;18(3):227-239
- 21. Ozec I, Kilic E, Gumus C, Goze F. Effect of local simvastatin application on mandibular defects. J Craniofac Surg 2007;18(3):546-550
- 22. Takagi K, Urist MR. The reaction of the dura to bone morphogenetic protein (BMP) in repair of skull defects. Ann Surg 1982;196(1):100-109
- 23. Al-Sulaimani AF, Mokeem SA, Anil S. Peri-implant defect augmentation with autogenous bone: A study in beagle dogs. J Oral Implantol 2013;39(1):30-36
- 24. Schuler RF, Janakievski J, Hacker BM. Effect of implant surface and grafting on implants placed into simulated extraction sockets: A histologic study in dogs. Int J Oral Maxillofac Implants 2010;25(5):893-900
- 25. Witso E, Persen L, Loseth K, Benum P, Bergh K. Cancellous bone as an antibiotic carrier. Acta Orthop Scand 2000;71(1):80-84
- 26. Kaya A, Kaya B, Aktas¸ A, Fırat ET. Effect of rifampin in combination with allogeneic, alloplastic, and heterogenous bone grafts on bone regeneration in rat tibial bone defects. J Oral Maxillofac Surg Med Pathol 2015;27(1):20-28
- 27. Rathbone CR, Cross JD, Brown KV. Effect of various concentrations of antibiotics on osteogenic cell viability and activity. J Orthop Res 2011;29(7):1070-1074
- 28. Zhang Z, Wang X, Luo F. Effects of rifampicin on osteogenic differentiation and proliferation of human mesenchymal stem cells in the bone marrow. Genet Mol Res 2014;13(3):6398-6410
- 29. Isefuku S, Joyner CJ, Simpson AH: Toxic effect of rifampicin on human osteoblast-like cells. J Orthop Res 2001;19(5):950-954
- 30. Wirries A, Schubert AK, Zimmermann R, Jabari S, Ruchholtz S, El-Najjar N. Black cumin oil accelerates osteoblast differentiation and activates bone morphogenetic protein-2 and ERK pathway. Int Immunopharmacol 2013;15(2):381-386
- 31. Kara MI, Erciyas K, Altan AB, Ozkut M, Ay S, Inan S. Thymoquinone accelerates new bone formation in the rapid maxillary expansion procedure. Arch Oral Biol. 2012;57(4):357-363
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Research Articles |
| Authors | |
| Project Number | DIS-158 |
| Publication Date | October 5, 2020 |
| Submission Date | April 9, 2020 |
| Published in Issue | Year 2020Volume: 23 Issue: 3 |
Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.
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