Objectives: Transcription factor nuclear factor-kappa B (NF-κB) is involved in the immune response against infection by a wide variety of cytokines and also plays a key role in gene expression. The aim of this study was to evaluate the gene polymorphism of NF-κB1(-94ins/del) in patients with chronic and aggressive periodontitis.Materials and Methods: 188 subjects, 75 AgP patients, 72 chronic periodontitis patients and 41 periodontally healty subjects, were included in our study. Blood samples were obtained from all subjects and DNA was isolated. Polymorphisms in NF-κB1(-94ins/del) were determined by polimeraz chain reaction- restriction fragment length polymorphism metod.Results: There was no significant difference in terms of age and gender among periodontitis patients enrolled in the study group and the control group (p> 0.05). Genotype distribution and allele frequencies of NF-κB1 gene in AgP and KP groups showed no significant difference in comparison with the control group (p> 0.05).Conclusions: Within the limits of this study, there was no association between NF-κB1 (-94 ins / del) promoter polymorphism and periodontal disease
Amaç: Transkripsiyon faktörü olan Nükleer Faktör-kappa B (NF-κB) enfeksiyona karşı immun cevapta rol alan çok çeşitli sitokinlerin gen ekspresyonunda anahtar rol oynar. Bu çalışmada kronik ve agresif periodontitis hastalarında NF-κB1(-94ins/del) gen polimorfizmi saptanması ve sağlıklı kontrol grubu ile karşılaştırılması amaçlanmıştır.
Metot: Çalışmamıza 75 AgP, 72 KP ve 41 periodontal açıdan sağlıklı toplam 188 birey dahil edildi. Hastalardan plak ve gingival indeks, cep derinliği ve ataşman seviyesi ölçümleri alındı. Tüm bireylerden venöz kan örnekleri toplandı ve DNA izolasyonu yapıldı. NF-κB polimorfizmi polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizmi(PZR-RFLP) yöntemi ile belirlendi.
Bulgular: Çalışmaya alınan periodontitis hasta grupları ile kontrol grubu arasında yaş ve cinsiyet açısından istatistiksel fark yoktu (p>0.05). NF-κB1 genine ait genotip dağılımları ve alel frekansları açısından AgP ve KP grupları ile kontrol grubu karşılaştırmalarında anlamlı farklılık saptanmadı (p>0.05) .
Sonuçlar: Bu çalışmanın sınırları dahilinde, NF-κB1 geni(-94 ins/del) promotor polimorfizmi ile agresif ve kronik periodontitis arasında ilişki bulunmamıştır.
Primary Language | Turkish |
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Journal Section | Original Research Articles |
Authors | |
Publication Date | July 7, 2014 |
Submission Date | July 7, 2014 |
Published in Issue | Year 2014Volume: 17 Issue: 4 |
Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.
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