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Cytotoxicity evaluation of methacrylate- and silorane-based composite resins

Year 2012, Volume: 15 Issue: 4, 327 - 334, 21.08.2012

Ozden Ozel Bektas , Digdem Eren , Gulsah Goktolga Akin , Zubeyde Akin Polat

https://doi.org/10.7126/cdj.2012.1699
Cited By: 2

Abstract

Objectives: The objective of this study was to investigate and compare the cytotoxic effects of four composite resin materials with different content.

Material and Methods: Two traditional methacrylate-based (Clearfil AP-X, RefleXions), as well as a self-adhering methacrylate-based (Vertise Flow) and a silorane-based (Filtek Silorane) composite resin were tested in the experiment. Ten cylindrical specimens were made of each material, using a mould (2mm. thick and 8 mm. in diameter). An agar diffusion method was employed, and cytotoxicity rankings were determined using lysis index scores. For statistical analysis, Kruskal-Wallis and Mann-Whitney U-tests were used.

Results: Amongst the composite resins, the silorane-based composite was found to be less cytotoxic than the methacrylate-based composite resins, which all had the same cytotoxicity ranking.

Conclusions: The silorane-based composite resin was considered more biocompatible than the methacrylate-based composite resins.

Keywords

Cytotoxicity methacrylate-based composite resin silorane-based composite resin self-adhesive composite resin

References

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  • - Geurtsen W, Lehmann F, Sphal W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblasts cultures. J Biomed Mater Res 1998; 41:474–80
  • - Spahl W, Budzikiewicz H, Geurtsen W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrophotometry. J Dent 1998; 26: 137–45
  • - Fong H, Dickens SH, Flaim GM. Evaluation of dental restorative composites containing polyhedral oligomeric silsesquioxane methacrylate. Dent Mater 2005; 21: 520-9
  • - Moszner N, Salz U. New developments of polymeric dental composites. Prog Polym Sci 2001; 26: 535–76.
  • - Yesilyurt C, Yoldas O, Altintas SH, Kusgoz A. Effects of food-simulating liquids on the mechanical properties of a silorane based dental composite. Dent Mater J 2009; 28(3):362-7
  • - Goldberg M. In vitro and in vivo studies on the toxicity of dental resin components: a review. Clin. Oral Invest. 2008; 12: 1–8.
  • - Ferracane JL. Resin composite—State of the art. Dent Mater 2011; 27(1):29-38
  • - Ernst C P, Meyer G R, Klöcker K, Willershausen B. Determination of polymerization shrinkage stress by means of a photoelastic investigation. Dent Mater 2004; 20: 313–21
  • - Ilie N, Jelen E, Clementino-Luedemann T, Hickel R. Low-shrinkage composite for dental application. Dent Mater J 2007;26: 149–155
  • - Palin WM, Fleming GJ, Burke FJ, Marquis P M, Randall RC. The influence of short and medium- term water immersion on the hydrolytic stability of novel low-shrink dental composites. Dent Mater 2005; 21: 852–63
  • - Furuse AY, Gordon K, Rodrigues FP, Silikas N, Watts DC. Colour-stability and gloss-retention of silorane and dimethacrylate composite with accelerated aging. J Dent 2008; 36: 945–52
  • - Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental restorative materials. J Oral Rehabil 1994; 21, 453–62
  • - Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991; 70, 1450–5
  • - Geurtsen W, Lehmann F, Sphal W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblasts cultures. J Biomed Mater Res 1998; 41:474–80
  • - Ausiello P, Cassese A, Miele C, Beguinot F, Garcia-Godoy F, Di Jeso B, Ulianich L. Cytotoxicity of dental resin composites: an in vitro evaluation J Appl Toxicol doi: 10.1002/jat.1765.
  • - Al-Hiyasat AS, Darmani H, Milhem MM. Cytotoxicity evaluation of dental resin composites and their flowable derivatives. Clin Oral Investig 2005; 9(1):21-5
  • - Schweikl H, Hiller KA, Bolay C, Kreissl M, Kreismann W, Nusser A, Steinhauser S, Wieczorek J, Vasold R, Schmalz G. Cytotoxic and mutagenic effects of dental composite materials. Biomaterials 2005; 26(14):1713-9
  • - Brackett MG, Bouillaguet S, Lockwood PE, Rotenberg S, Lewis JB, Messer RLW, Wataha JC. In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries. J Biomed Mater Res Part B: Appl Biomater 2007; 81(2):397-402.
  • - Krifka S, Seidenader C, Hiller KA, Schmalz G, Schweikl H. Oxidative stress and cytotoxicity generated by dental composites in human pulp cells. Clin Oral Investig 2012; 16(1):215-24.
  • - Wataha JC. Principles of biocompatibility for dental practitioners. J Prosthet Dent 2001; 86(2):203-9
  • - Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dent Mater 1996; 12: 186-93
  • - Spangberg LSW. Correlation of in vivo and in vitro screening tests. J Endodont 1978; 4: 296–9
  • - Hodgson E. Measurement of toxicity. In Hodgson E, Levi PE, editors. Modern toxicology. New York (NY): Elsevier; 1987. p. 233-85.
  • - Odabaş ME, Ertürk M, Çınar Ç, Tüzüner T, Tulunoğlu Ö. Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro. Med Oral Patol Oral Cir Bucal 2011; 16(4):e584-7.
  • - Cao T, Saw TY, Heng BC, Liu H, Yap AU, Ng ML. Comparison of different test models for the assessment of cytotoxicity of composite resins. J Appl Toxicol 2005; 25(2):101-8.
  • - Schedle A, Franz A, Rausch-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dent Mater 1998; 14: 429–40
  • - Franz A, König F, Anglmayer M, Rausch-Fan X, Gille G, Rausch WD, Lucas T, Sperr W, Schedle A. Cytotoxic effects of packable and nonpackable dental composites. Dent Mater 2003; 19: 382–92
  • - Sjögren G, Sletten GBG, Dahl JE. Cytotoxicity of dental alloys, metals, and ceramics assessed by Millipore filter, agar overlay and MTT tests. J Prosthet Dent 2000; 84: 229–36
  • - Ulker HE, Sengun A. Cytotoxicity Evaluation of Self Adhesive Composite Resin Cements by Dentin Barrier Test on 3D Pulp Cells. Eur J Dent 2009; 3(2):120-6
  • - Tunçel A, Ozdemir AK, Sümer Z, Hürmüzlü F, Polat Z. Cytotoxicity evaluation of two different composites with/without fibers and one nanohybrid composite. Dent Mater J 2006; 25(2):267-71
  • - Bouillaguet S, Shaw L, Gonzalez L, Wataha JC, Krejci I. Long-term cytotoxicity of resin-based dental restorative materials. J Oral Rehabil 2002;29(1):7-13
  • - Wataha JC, Lockwood PE, Bouillaguet S, Noda M. In vitro biological response to core and flowable dental restorative materials. Dent Mater 2003; 19: 25-31
  • - Caughman WF, Caughman GB, Shiflett RA, Rueggeberg F, Schuster GS. Correlation of cytotoxicity, filler loading and curing time of dental composites. Biomaterials 1991;12: 737-40
  • - Ergun G, Egilmez F, Cekic-Nagas I. The cytotoxicity of resin composites cured with three light curing units at different curing distances Med Oral Patol Oral Cir Bucal 2011;16(2):e252-9.
  • - Papadogiannis D, Tolidis K, Lakes R, Papadogiannis Y. Viscoelastic properties of low-shrinking composite resins compared to packable composite resins. Dent Mater J 2011; 30(3):350-7
  • - Castañeda ER, Silva LA, Gaton-Hernández P, Consolaro A, Rodriguez EG, Silva RA, Queiroz AM, Nelson-Filho P. FiltekTM Silorane and FiltekTM Supreme XT Resins: Tissue Reaction After Subcutaneous Implantation in Isogenic Mice. Braz Dent J 2011; 22(2):105-10.
  • - Eick JD, Smith RE, Pinzino CS, Kostoryz EL. Stability of silorane dental monomers in aqueous systems. J Dent 2006; 34: 405-10
  • - Rueggeberg FA, Margeson DH. The effect of oxygen inhibition on an unfilled/filled composite system. Journal of Dental Research 1990; 69: 1652-8
  • - Gauthier MA, Stangel I, Ellis TH, Zhu XX. Oxygen inhibition in dental resins. J Dent Res 2005;84(8):725-9
  • - Caughman WF, Caughman GB, Shiflett RA, Rueggeberg F, Schuster GS. Correlation of cytotoxicity, filler loading and curing time of dental composites. Biomaterials 1991;12: 737-40

Year 2012, Volume: 15 Issue: 4, 327 - 334, 21.08.2012

Ozden Ozel Bektas , Digdem Eren , Gulsah Goktolga Akin , Zubeyde Akin Polat

https://doi.org/10.7126/cdj.2012.1699
Cited By: 2

Abstract

References

  • REFERENCES
  • - Weinmann W, Thalacker C, Guggenberger R. Siloranes in dental composites. Dent Mater 2005; 21:68–74
  • - Geurtsen W, Lehmann F, Sphal W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblasts cultures. J Biomed Mater Res 1998; 41:474–80
  • - Spahl W, Budzikiewicz H, Geurtsen W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrophotometry. J Dent 1998; 26: 137–45
  • - Fong H, Dickens SH, Flaim GM. Evaluation of dental restorative composites containing polyhedral oligomeric silsesquioxane methacrylate. Dent Mater 2005; 21: 520-9
  • - Moszner N, Salz U. New developments of polymeric dental composites. Prog Polym Sci 2001; 26: 535–76.
  • - Yesilyurt C, Yoldas O, Altintas SH, Kusgoz A. Effects of food-simulating liquids on the mechanical properties of a silorane based dental composite. Dent Mater J 2009; 28(3):362-7
  • - Goldberg M. In vitro and in vivo studies on the toxicity of dental resin components: a review. Clin. Oral Invest. 2008; 12: 1–8.
  • - Ferracane JL. Resin composite—State of the art. Dent Mater 2011; 27(1):29-38
  • - Ernst C P, Meyer G R, Klöcker K, Willershausen B. Determination of polymerization shrinkage stress by means of a photoelastic investigation. Dent Mater 2004; 20: 313–21
  • - Ilie N, Jelen E, Clementino-Luedemann T, Hickel R. Low-shrinkage composite for dental application. Dent Mater J 2007;26: 149–155
  • - Palin WM, Fleming GJ, Burke FJ, Marquis P M, Randall RC. The influence of short and medium- term water immersion on the hydrolytic stability of novel low-shrink dental composites. Dent Mater 2005; 21: 852–63
  • - Furuse AY, Gordon K, Rodrigues FP, Silikas N, Watts DC. Colour-stability and gloss-retention of silorane and dimethacrylate composite with accelerated aging. J Dent 2008; 36: 945–52
  • - Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental restorative materials. J Oral Rehabil 1994; 21, 453–62
  • - Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991; 70, 1450–5
  • - Geurtsen W, Lehmann F, Sphal W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblasts cultures. J Biomed Mater Res 1998; 41:474–80
  • - Ausiello P, Cassese A, Miele C, Beguinot F, Garcia-Godoy F, Di Jeso B, Ulianich L. Cytotoxicity of dental resin composites: an in vitro evaluation J Appl Toxicol doi: 10.1002/jat.1765.
  • - Al-Hiyasat AS, Darmani H, Milhem MM. Cytotoxicity evaluation of dental resin composites and their flowable derivatives. Clin Oral Investig 2005; 9(1):21-5
  • - Schweikl H, Hiller KA, Bolay C, Kreissl M, Kreismann W, Nusser A, Steinhauser S, Wieczorek J, Vasold R, Schmalz G. Cytotoxic and mutagenic effects of dental composite materials. Biomaterials 2005; 26(14):1713-9
  • - Brackett MG, Bouillaguet S, Lockwood PE, Rotenberg S, Lewis JB, Messer RLW, Wataha JC. In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries. J Biomed Mater Res Part B: Appl Biomater 2007; 81(2):397-402.
  • - Krifka S, Seidenader C, Hiller KA, Schmalz G, Schweikl H. Oxidative stress and cytotoxicity generated by dental composites in human pulp cells. Clin Oral Investig 2012; 16(1):215-24.
  • - Wataha JC. Principles of biocompatibility for dental practitioners. J Prosthet Dent 2001; 86(2):203-9
  • - Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dent Mater 1996; 12: 186-93
  • - Spangberg LSW. Correlation of in vivo and in vitro screening tests. J Endodont 1978; 4: 296–9
  • - Hodgson E. Measurement of toxicity. In Hodgson E, Levi PE, editors. Modern toxicology. New York (NY): Elsevier; 1987. p. 233-85.
  • - Odabaş ME, Ertürk M, Çınar Ç, Tüzüner T, Tulunoğlu Ö. Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro. Med Oral Patol Oral Cir Bucal 2011; 16(4):e584-7.
  • - Cao T, Saw TY, Heng BC, Liu H, Yap AU, Ng ML. Comparison of different test models for the assessment of cytotoxicity of composite resins. J Appl Toxicol 2005; 25(2):101-8.
  • - Schedle A, Franz A, Rausch-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dent Mater 1998; 14: 429–40
  • - Franz A, König F, Anglmayer M, Rausch-Fan X, Gille G, Rausch WD, Lucas T, Sperr W, Schedle A. Cytotoxic effects of packable and nonpackable dental composites. Dent Mater 2003; 19: 382–92
  • - Sjögren G, Sletten GBG, Dahl JE. Cytotoxicity of dental alloys, metals, and ceramics assessed by Millipore filter, agar overlay and MTT tests. J Prosthet Dent 2000; 84: 229–36
  • - Ulker HE, Sengun A. Cytotoxicity Evaluation of Self Adhesive Composite Resin Cements by Dentin Barrier Test on 3D Pulp Cells. Eur J Dent 2009; 3(2):120-6
  • - Tunçel A, Ozdemir AK, Sümer Z, Hürmüzlü F, Polat Z. Cytotoxicity evaluation of two different composites with/without fibers and one nanohybrid composite. Dent Mater J 2006; 25(2):267-71
  • - Bouillaguet S, Shaw L, Gonzalez L, Wataha JC, Krejci I. Long-term cytotoxicity of resin-based dental restorative materials. J Oral Rehabil 2002;29(1):7-13
  • - Wataha JC, Lockwood PE, Bouillaguet S, Noda M. In vitro biological response to core and flowable dental restorative materials. Dent Mater 2003; 19: 25-31
  • - Caughman WF, Caughman GB, Shiflett RA, Rueggeberg F, Schuster GS. Correlation of cytotoxicity, filler loading and curing time of dental composites. Biomaterials 1991;12: 737-40
  • - Ergun G, Egilmez F, Cekic-Nagas I. The cytotoxicity of resin composites cured with three light curing units at different curing distances Med Oral Patol Oral Cir Bucal 2011;16(2):e252-9.
  • - Papadogiannis D, Tolidis K, Lakes R, Papadogiannis Y. Viscoelastic properties of low-shrinking composite resins compared to packable composite resins. Dent Mater J 2011; 30(3):350-7
  • - Castañeda ER, Silva LA, Gaton-Hernández P, Consolaro A, Rodriguez EG, Silva RA, Queiroz AM, Nelson-Filho P. FiltekTM Silorane and FiltekTM Supreme XT Resins: Tissue Reaction After Subcutaneous Implantation in Isogenic Mice. Braz Dent J 2011; 22(2):105-10.
  • - Eick JD, Smith RE, Pinzino CS, Kostoryz EL. Stability of silorane dental monomers in aqueous systems. J Dent 2006; 34: 405-10
  • - Rueggeberg FA, Margeson DH. The effect of oxygen inhibition on an unfilled/filled composite system. Journal of Dental Research 1990; 69: 1652-8
  • - Gauthier MA, Stangel I, Ellis TH, Zhu XX. Oxygen inhibition in dental resins. J Dent Res 2005;84(8):725-9
  • - Caughman WF, Caughman GB, Shiflett RA, Rueggeberg F, Schuster GS. Correlation of cytotoxicity, filler loading and curing time of dental composites. Biomaterials 1991;12: 737-40
There are 42 citations in total.

Details

Primary Language English
Journal Section Original Research Articles
Authors

Ozden Ozel Bektas

Digdem Eren

Gulsah Goktolga Akin

Zubeyde Akin Polat

Publication Date August 21, 2012
Submission Date August 21, 2012
Published in Issue Year 2012Volume: 15 Issue: 4

Cite

EndNote Ozel Bektas O, Eren D, Goktolga Akin G, Akin Polat Z (October 1, 2012) Cytotoxicity evaluation of methacrylate- and silorane-based composite resins. Cumhuriyet Dental Journal 15 4 327–334.

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Anuj Bhardwaj
https://doi.org/10.20473/cdj.v8i2.2018.123-129

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