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Sodyum hipoklorit, klorheksidin ve propolis içerikli solüsyonların potasyum titanyum fosfat lazer ile birlikte kullanımlarının candida albicans üzerine etkinliklerinin incelenmesi

Year 2009, Volume: 12 Issue: 1, 33 - 38, 01.11.2011

Ulku Ozan İhsan Hubbezoglu Zeynep Sumer

Abstract

Aim: Antimicrobial effects of sodium hypochlorite, chlorhexidine gluconate (CHX) and different concentrations of propolis solutions (PS) were evaluated on Candida albicans in root canals. In the next step, after application of irrigation solutions antimicrobial effect of Potassium Titanium Phosphate (KTP) laser against C. albicans was tested.

Material and Methods: Freshly extracted forty-two single rooted human teeth were divided into 6 equal groups; And selected irrigants and application were for Group 1 NaOCl, Group 2 CHX, Group 3 5%PS, Group 4 10%PS, Group 5 20%PS, Group 6 Saline solution (control group). Roots with predetermined lengths were placed in glass bottles. Then, the roots were shaped using ProTaper files by crown down technique. Then the teeth sterilized by ethylene dioxide and contaminated with C. albicans. After then, 2 ml of irrigation solution was used for each teeth. 24h growth were determined by evaluation of turbidity in test tubes. As a final step, KTP laser was applied to root canals and microbial growth were determined. The results were evaluated using Kruskal Wallis, Mann Whitney-U, Chi square and Friedman tests.

Results: All tested irrigation solutions (except for control group) were found statistically effective against C. albicans with regard to antimicrobial efficacy (p<0.05). After having done irrigation in the root canals, application of KTP laser with propolis %20, propolis %10 and SF was found statistically effective with regard to antimicrobial efficacy (p<0.05).

Conclusion: In root canals, propolis had antimicrobial effect, however results were unsatisfactory. Furthermore, addition of KTP laser energy gave stronger antimicrobial effect.

ÖZET

Amaç: Kök kanalına uygulanan, sodyum hipoklorit (NaOCl), klorheksidin glukonat (KHG) ve farklı konsantrasyonlarda hazırlanmış propolis solüsyonlarının (PS) Candida albicans üzerine antimikrobiyal etkinliklerinin karşılaştırılmasıdır. Ayrıca Potasyum Titanyum Fosfat lazerin (KTP) irrigasyon solüsyonlarından sonra uygulanmasının C. albicans üzerine antimikrobiyal etkinliğini değerlendirmektir.

Gereç ve Yöntem: 42 adet yeni çekilmiş tek köklü insan dişi, her biri 7 dişten oluşan alt gruplara ayrıldı. Grup 1 NaOCl, Grup 2 KHG, Grup 3 %5’lik PS, Grup 4 %10’luk PS Grup 5 %20’lik PS, Grup 6 Serum fizyolojik (kontrol grubu). Çalışma boyutları hesaplanan dişler lastik kapaklı cam şişelere yerleştirildi. ProTaper kök kanal aletleri ile crowndown tekniği kullanılarak prepare edildi. Cam şişelerdeki steril dişler aminar air flow’da C. albicans’la kontamine edildi. Her diş için 2 ml irrigasyon solusyonu kullanıldı. 24 saat sonra bulanıklıklarına bakılarak sonuçlar değerlendirildi. Son aşama olarak kök kanallarına KTP lazer uygulandı ve mikrobiyal üreme olup olmadığı belirlendi. Veriler değerlendirilirken Kruskal Wallis testi, Mann Whitney-U testi, Chi square, Friedman testi ve Wilcoxon testi uygulanmıştır.

Bulgular: Tüm test edilen irrigasyon solüsyonlarının (kontrol grubu hariç) C. albicans’a karşı antimikotik etkinlik yönünden istatistiksel olarak önemli bulundu (p<0,05). Propolis %20, Propolis %10 ve SF (kontrol gurubu) ile kök kanallarında irrigasyon yapıldıktan sonra KTP lazer uygulaması antimikotik etkinlik yönünden istatistiksel olarak önemli bulundu (p<0,05).

Sonuç: Kök kanallarında, propolisin, antimikrobiyal etkisi olmasına rağmen istenilen yeterlilikte bulunamadı. Ayrıca irrigasyon solüsyonlarından sonra, KTP lazer uygulanmasının daha etkili antimikrobiyal etki gösterebileceği belirlendi.

Anahtar kelimeler: Propolis, irrigasyon, lazer, mikroorganizma

Keywords

Propolis; irrigation; laser; microorganism

References

  • Fabricius L, Dahlén G, Sundqvist G, Happonen RP, Möller AJR. Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci 2006; 114 : 278-85.
  • Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 1997; 30: 297-306.
  • Sen BH, Safavi KE, SpaEngberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997; 84: 68-73.
  • Waltimo TM, Sire´n EK, Torkko HL, Olsen I, Haapasalo MP. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997; 30: 96-101.
  • Smith JJ, Wayman BE. An evaluation of the antimicrobial effectiveness of citric aid as a root canal irrigant. J Endod 1986; 12: 54-7.
  • Hepen 1 F, Tilgen F, Er H. Propolis: Tbbi özellikleri ve oftalmolojik kullanm. Turgut
  • Özal Merkezi Tp Dergisi 1996; 3: 386-91.
  • Lahinler N. Ar ürünleri ve insan sal açsndan önemi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi 2000; 5: 139-48.
  • Kutluca S. Propolis üretim yöntemlerinin koloni performans ve propolisin kimyasal özellikleri üzerine etkileri. Atatürk Üniversitesi Fen Bilimleri Enstitüsü. (Doktora tezi), Erzurum, 2003; 145.
  • Moshonov J, Orstavik D, Yamauchi S, Pettiette M, Trope M. Nd:YAG laser irridation in root canal disinfection. Endod Dent Traumatol 1995; 11: 220-4.
  • Klinke T, Klimm W, Gutknecht N. Antibacterial effects of Nd:YAG laser irradiation within root canal dentin. J Clin Laser Med Surg 1997; 15: 29–31.
  • Moritz A, Doertbudak O, Guetknecht N, Goharkay K,Schoop U, Sperr W. Nd:YAG laser irradiation of infected root canals in combination with microbiological examinations. J Am Dent Assoc 1997; 128: 1525-30.
  • Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia 2002; 73: 1-6.
  • Sen BH, Safavi KE, SpaÊngberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997; 84: 68-73.
  • Waltimo TM, Ørstavik D, Siren EK, Haapasalo MP. In vitro susceptibility of Candida albicans to four disinfectants and their combinations. Int Endod J 1999; 32: 421-9.
  • Koo H, Gomes BPFA, Rosalen PL, Ambrosano GMB, Park YK, Cury JA. In vitro antimicrobial activity of propolis and arnica montana against oral pathogens. Arch Oral Biol 2000; 45: 141-8.
  • Koo H, Vacca Smith AM, Bowen WH, Rosalen PL, Cury JA, Park YK. Effects of apis mellifera propolis on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated hydroxyapatite. Caries Res 2000; 34: 418-26.
  • Koo H, Rosalen PL, Cury JA, Ambrosano GM, Murata RM, Yatsuda R, Ikegaki M, Alencar SM, Park YK. Effect of a new variety of Apis mellifera propolis on mutans streptococci. Curr Microbiol 2000; 41: 192–6.
  • Koo H, Rosalen PL, Cury JA, Park YK, Ikegaki M, Sattler A. Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats. Caries Res 1999; 33: 393–400.
  • Yazcolu A, Deer O, Ovali E, Barlak Y, Hosver I, Tekeliplu Y, Karahan SC. Effects of Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines. Int Immunopharmacol 2005; 5: 1652–7.
  • Al-Shader A, Wallace J, Agarwal S, Bretz W, Baugh D. Effect of propolis on human fibroblasts from the pulp and periodontal ligament. J Endod 2004; 30: 359–61.
  • Waltimo TM, Sire´n EK, Torkko HL, Olsen I, Haapasalo MP. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997; 30: 96-101.
  • Ramskold LO, Fong CD, Stromberg T. Thermal effects and antibacterial properties of energy levels required to sterilize stained root canals with an Nd:YAG laser. J Endod 1997; 23: 96-100.
  • Ballal V, Kundabala M, Acharya S, Ballal M. Antimicrobial action of calcium hydroxide, chlorhexidine and their combination on endodontic pathogens. Aust Dent J 2007; 52: 118-21.
  • D'Arcangelo C, Varvara G, De Fazio P. An evaluation of the action of different root canal irrigants on facultative aerobicanaerobic, obligate anaerobic, and microaerophilic bacteria. J Endod 1999; 25: 351-3.
  • Dumani A, Yoldas O, Isci AS, Köksal F, Kayar B, Polat E. Disinfection of artificially contaminated Resilon cones with chlorhexidine and sodium hypochlorite at different time exposures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 103: 82-5.
  • Kartal M, Yldz S, Kaya S, Kurucu S, Topçu G. Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol 2003; 86: 69-73.
  • Uzel A, Sorkun K, Önça Ö, Çoulu D, Gencay Ö, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples Microbiol Res 2005; 160: 189-95.
  • Stepanovif S, Antif N, Dakif I, Švabif-Vlahovif M. In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 2003; 158: 353-7.
  • Haapasalo M, Endal U, Zandi H, Coil JM. Eradication of endodontic infection by instrumentation and irrigation solutions. Endod Topics 2005; 10: 77–102.
  • Kimura Y, Arrastia-Jitosho AMA, Wilder-Smith P. Thermal, microstructural and physicochemical effects of nanosecond pulsed Nd: YAG laser irradiation on dentin. Laser Life Sci 1998; 8: 37–50.
  • Kimura Y, Wilder-Smith P, Arrastia-Jitosho AMA, Liaw L-HL, Matsumoto K, Berns MW. Effects of nanosecond pulsed Nd: YAG laser irradiation on dentin resistance to artificial caries-like lesions. Lasers Surg Med 1997; 20:15–21.

SODYUM H POKLOR T, KLORHEKS D N VE PROPOL S ÇER KL SOLÜSYONLARIN POTASYUM T TANYUM FOSFAT LAZER LE B RL KTE KULLANIMLARININ CAND DA ALB CANS ÜZER NE ETK NL KLER N N NCELENMES

Year 2009, Volume: 12 Issue: 1, 33 - 38, 01.11.2011

Ulku Ozan İhsan Hubbezoglu Zeynep Sumer

Abstract

Amaç: Kök kanal na uygulanan, sodyum hipoklorit (NaOCl), klorheksidin glukonat (KHG) ve farkl konsantrasyonlarda haz rlanm propolis solüsyonlar n n (PS) Candida albicans üzerine antimikrobiyal etkinliklerinin kar la t r lmas d r. Ayr ca Potasyum Titanyum Fosfat lazerin (KTP) irrigasyon solüsyonlar ndan sonra uygulanmas n n C. albicans üzerine antimikrobiyal etkinli+ini de+erlendirmektir. Gereç ve Yöntem: 42 adet yeni çekilmi tek köklü insan di i, her biri 7 di ten olu an alt gruplara ayr ld . Grup 1 NaOCl, Grup 2 KHG, Grup 3 %5’lik PS, Grup 4 %10’luk PS Grup 5 %20’lik PS, Grup 6 Serum fizyolojik (kontrol grubu). Çal ma boyutlar hesaplanan di ler lastik kapakl cam i elere yerle tirildi. ProTaper kök kanal aletleri ile crowndown tekni+i kullan larak prepare edildi. Cam i elerdeki steril di ler laminar air flow’da C. albicans’la kontamine edildi. Her di için 2 ml irrigasyon solusyonu kullan ld . 24 saat sonra bulan kl klar na bak larak sonuçlar de+erlendirildi. Son a ama olarak kök kanallar na KTP lazer uyguland ve mikrobiyal üreme olup olmad + belirlendi. Veriler de+erlendirilirken Kruskal Wallis testi, Mann Whitney-U testi, Chi square, Friedman testi ve Wilcoxon testi uygulanm t r. Bulgular: Tüm test edilen irrigasyon solüsyonlar n n (kontrol grubu hariç) C. albicans’a kar antimikotik etkinlik yönünden istatistiksel olarak önemli bulundu (p

Keywords

propolis irrigasyon lazer mikroorganizma

References

  • Fabricius L, Dahlén G, Sundqvist G, Happonen RP, Möller AJR. Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci 2006; 114 : 278-85.
  • Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 1997; 30: 297-306.
  • Sen BH, Safavi KE, SpaEngberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997; 84: 68-73.
  • Waltimo TM, Sire´n EK, Torkko HL, Olsen I, Haapasalo MP. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997; 30: 96-101.
  • Smith JJ, Wayman BE. An evaluation of the antimicrobial effectiveness of citric aid as a root canal irrigant. J Endod 1986; 12: 54-7.
  • Hepen 1 F, Tilgen F, Er H. Propolis: Tbbi özellikleri ve oftalmolojik kullanm. Turgut
  • Özal Merkezi Tp Dergisi 1996; 3: 386-91.
  • Lahinler N. Ar ürünleri ve insan sal açsndan önemi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi 2000; 5: 139-48.
  • Kutluca S. Propolis üretim yöntemlerinin koloni performans ve propolisin kimyasal özellikleri üzerine etkileri. Atatürk Üniversitesi Fen Bilimleri Enstitüsü. (Doktora tezi), Erzurum, 2003; 145.
  • Moshonov J, Orstavik D, Yamauchi S, Pettiette M, Trope M. Nd:YAG laser irridation in root canal disinfection. Endod Dent Traumatol 1995; 11: 220-4.
  • Klinke T, Klimm W, Gutknecht N. Antibacterial effects of Nd:YAG laser irradiation within root canal dentin. J Clin Laser Med Surg 1997; 15: 29–31.
  • Moritz A, Doertbudak O, Guetknecht N, Goharkay K,Schoop U, Sperr W. Nd:YAG laser irradiation of infected root canals in combination with microbiological examinations. J Am Dent Assoc 1997; 128: 1525-30.
  • Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia 2002; 73: 1-6.
  • Sen BH, Safavi KE, SpaÊngberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997; 84: 68-73.
  • Waltimo TM, Ørstavik D, Siren EK, Haapasalo MP. In vitro susceptibility of Candida albicans to four disinfectants and their combinations. Int Endod J 1999; 32: 421-9.
  • Koo H, Gomes BPFA, Rosalen PL, Ambrosano GMB, Park YK, Cury JA. In vitro antimicrobial activity of propolis and arnica montana against oral pathogens. Arch Oral Biol 2000; 45: 141-8.
  • Koo H, Vacca Smith AM, Bowen WH, Rosalen PL, Cury JA, Park YK. Effects of apis mellifera propolis on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated hydroxyapatite. Caries Res 2000; 34: 418-26.
  • Koo H, Rosalen PL, Cury JA, Ambrosano GM, Murata RM, Yatsuda R, Ikegaki M, Alencar SM, Park YK. Effect of a new variety of Apis mellifera propolis on mutans streptococci. Curr Microbiol 2000; 41: 192–6.
  • Koo H, Rosalen PL, Cury JA, Park YK, Ikegaki M, Sattler A. Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats. Caries Res 1999; 33: 393–400.
  • Yazcolu A, Deer O, Ovali E, Barlak Y, Hosver I, Tekeliplu Y, Karahan SC. Effects of Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines. Int Immunopharmacol 2005; 5: 1652–7.
  • Al-Shader A, Wallace J, Agarwal S, Bretz W, Baugh D. Effect of propolis on human fibroblasts from the pulp and periodontal ligament. J Endod 2004; 30: 359–61.
  • Waltimo TM, Sire´n EK, Torkko HL, Olsen I, Haapasalo MP. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997; 30: 96-101.
  • Ramskold LO, Fong CD, Stromberg T. Thermal effects and antibacterial properties of energy levels required to sterilize stained root canals with an Nd:YAG laser. J Endod 1997; 23: 96-100.
  • Ballal V, Kundabala M, Acharya S, Ballal M. Antimicrobial action of calcium hydroxide, chlorhexidine and their combination on endodontic pathogens. Aust Dent J 2007; 52: 118-21.
  • D'Arcangelo C, Varvara G, De Fazio P. An evaluation of the action of different root canal irrigants on facultative aerobicanaerobic, obligate anaerobic, and microaerophilic bacteria. J Endod 1999; 25: 351-3.
  • Dumani A, Yoldas O, Isci AS, Köksal F, Kayar B, Polat E. Disinfection of artificially contaminated Resilon cones with chlorhexidine and sodium hypochlorite at different time exposures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 103: 82-5.
  • Kartal M, Yldz S, Kaya S, Kurucu S, Topçu G. Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol 2003; 86: 69-73.
  • Uzel A, Sorkun K, Önça Ö, Çoulu D, Gencay Ö, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples Microbiol Res 2005; 160: 189-95.
  • Stepanovif S, Antif N, Dakif I, Švabif-Vlahovif M. In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 2003; 158: 353-7.
  • Haapasalo M, Endal U, Zandi H, Coil JM. Eradication of endodontic infection by instrumentation and irrigation solutions. Endod Topics 2005; 10: 77–102.
  • Kimura Y, Arrastia-Jitosho AMA, Wilder-Smith P. Thermal, microstructural and physicochemical effects of nanosecond pulsed Nd: YAG laser irradiation on dentin. Laser Life Sci 1998; 8: 37–50.
  • Kimura Y, Wilder-Smith P, Arrastia-Jitosho AMA, Liaw L-HL, Matsumoto K, Berns MW. Effects of nanosecond pulsed Nd: YAG laser irradiation on dentin resistance to artificial caries-like lesions. Lasers Surg Med 1997; 20:15–21.
There are 32 citations in total.

Details

Primary Language English
Journal Section Original Research Articles
Authors

Ulku Ozan

İhsan Hubbezoglu

Zeynep Sumer

Publication Date November 1, 2011
Submission Date November 1, 2011
Published in Issue Year 2009Volume: 12 Issue: 1

Cite

EndNote Ozan U, Hubbezoglu İ, Sumer Z (November 1, 2011) Sodyum hipoklorit, klorheksidin ve propolis içerikli solüsyonların potasyum titanyum fosfat lazer ile birlikte kullanımlarının candida albicans üzerine etkinliklerinin incelenmesi. Cumhuriyet Dental Journal 12 1 33–38.

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


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