Research Article
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REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS

Year 2021, , 216 - 223, 15.09.2021

Ismail Hutasoit , Agy Pranata Masna Wiyah , Fitri Ramadhani , Hubban Nasution

https://doi.org/10.7126/cumudj.887101

Abstract

Objectives: This study aimed to identify porcine DNA in prosthodontic materials using Real-Time PCR.
Materials and methods: Eighteen prosthodontic materials: three irreversible hydrocolloids, three elastomers, three denture adhesives, three soft denture linings, three temporary crowns, and three denture bases materials were used as the samples. It was conducted in two stages. First, extraction of dental material’s DNA and the second was Real-Time PCR analysis based on amplification curve and Ct score in yellow and green channel.
Results: The sample analysis based on green channel demonstrated that all materials did not contain porcine DNA, however, 11 of 18 samples (DA-01, DA-02, DA-03, SDL-02, DB-01, DB-02, TC-03, IH-02, EM-01, EM-02, and EM-03) contained vertebrate DNA. Conclusions: All prosthodontic materials tested were not containing porcine. The halal statues of the materials were still unclear.

Keywords

pcr Porcine DNA prosthodontic materials

References

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  • 2. Rahardian B, Hakim N, Kurniawan A. Implementation of halal product guarantee in dental health services in Islamic hospital. Int J Human and Health Sci 2019; 3(2): 54-7.
  • 3. Anusavice KJ. Philips’s science of dental materials. 12th ed. Missouri: Elsevier, 2013:5-6.
  • 4. Faaz, Adeel, Zohaib. Application of metals and alloys in dentistry. In: Khurshid Z, Sheikh Z, ed. Dental Materials (Principles and Applications). Pakistan: Paramount Books Ltd, 2018: 41.
  • 5. Chalifoux PR. Acrylic and other resin: provisional restorations. In: Aschheim KW. eds. Esthetic Dentistry: A Clinical Approach to Techniques and Materials, 3rd. St. Louis: Mosby, 2015;197-203.
  • 6. Nafis MC. The concept of halal and thayyib and its implementation in Indonesia. JHPR 2019; 2(1): 1-5. 7. Khan MI, Haleem A. Understanding “halal” and “halal certification & accreditation system”-brief review. Saudi J. Bus. Manag. Stud. 2016; 1(1): 32-42.
  • 8. Salim M. Contemporary Islamic law in Indonesia-sharia and legal pluralism. Edinburgh: Edinburgh University Press, 2015: 23-27.
  • 9. JM Muslimin. Halal Product Guarantee in Indonesia Regulation and Social Inclusion. IJEB 2019; 4: 27-29.
  • 10. Ali E, Sultana S, Hamid SBA, Hossain M, Yehya WA, et al. Gelatin controversies in food, pharmaceuticals, and personal care product: authentication methods, current status, and future challenges. University of Malaya 2017; 1-10.
  • 11. Ali ME, Kashif M., Uddin K, Hashim U., Mustafa S., Man YBC. Species authentication methods in foods and feeds: the present, past, and future of halal forensics. Food anal. Methods 2012; 5(1): 946.
  • 12. Roswiem AP, Mustaqimah DN. Identification of gelatin source in toothpaste products using combination of attenuated total reflection-fourier transform infrared (ATR-FTIR) spectroscopy and chemometrics. IJOHR 2020; 2(1): 30-37.
  • 13. Hermanto S, Sumartin LO, Fatimah W. Differentiation of bovine and porcine gelatin based on spectroscopic and electrophoretic analysis. J Food Pharm Sci 2013. 1. 68-73.
  • 14. Lee JH, Yi GY, Lee JW. Physicochemical characterization of porcine bone-derived grafting material and comparison with bovine xenografts for dental applications. JPIS 2017; 47(6): 388.
  • 15. Sudjadi, Wardani HS, Sepminarti T, Rohman A. Analysis of porcine gelatin DNA in a commercial capsule shell using Real Time polymerase chain reaction for halal authentication. IJFP 2016; 19: 2127-34.
  • 16. Rocha AJ, Monteiro-Júnior JE, Freire JEC, Sousa AJS, Fonteles CSR. Real-time PCR: the use of reference genes and essential rules required to obtain normalization data reliable to quantitative gene expression. JMBR 2015; 5(1): 45-55.
  • 17. Corona B, Lleonard R, Carpio Y, Uffo O, Martinez. Short communication, PCR detection of DNA bovine, ovine-caprine and porcine origin in feed as part a bovine spongiform encephalopathy control program. SJAR 2017; 5(3): 312-7.
  • 18. Erwanto, et al. Identification of pig DNA in product using polymerase chain reaction (PCR) for halal authentication – a review. IFRJ 2018; 25(4): 22–31.
  • 19. Loftis AD, Reeves WK. Principles of Real-Time PCR. In: Wang C, Kaltenboeck B, Freeman MD. eds. Veterinary PCR diagnostics, Basseterre: Bhentam E Books , 2012: 3-6.
  • 20. Salamah N, Erwanto Y, Martono S, Rohman A. Real-Time PCR-based detection of bovine DNA by specific targeting on cytochrome-B. JUAD 2019; 9(2): 201-08.
  • 21. Halib N, Suhaila NS, Fattah WA, et al. Islam and technological development in Malaysia’s health care: an Islam legal basis analysis of dental material used in periodontal theraphy. Mal J of So Sp. 2016; 12 (1): 88-95.
  • 22. Ifranita N, Mirghani I, Saeed ME, et al. Rapid detection of gelatin in dental materials using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR). J. Phys: Conf. Ser. 2017; 884: 1-10.
  • 23. Azrul Aziz AH, Ab Mutalib NA, Rahman RA, et al. The authentication of halal dental materials using rapid fourier transform infrared (FTIR) spectroscopy. Adv Sci. 2017; 23(5): 1-4.
  • 24. Craig’s, Powers JM, Sakaguchi RL 14th ed. Restorative dental materials. India. Mosby Elsevier. 2009; 150-204.
  • 25. Gounder R, Sathasivam P. Importance of denture adhesive in complete denture patiens-a review. IJRAMR 2016; 3 (6): 35-37.
  • 26. Manar J. Alginate as impression material. Int J Appl Dent Sci 2018; 4 (3): 300-301.
  • 27. Hatrick CD, Eakle S, Bird WF. Dental materials - clinical applications for dental assitants and dental hygienist. 3rd ed. Louis: Saunders Elsevier, 2015: 97, 106-7.
  • 28. Zarb B. Prosthodontic treatment for edentulous patient complete denture and implant-suported prosthesis. 5th ed. Amsterdam: Elsevier, 2011:442-48.
  • 29. Bobkowska BD, Medyriski D, Pryliriski M. Recent advances in tissue conditioners for prosthetic treatment: a review. Wroclaw medical university 2016; 724.
  • 30. Widayat, Agustini TW, Suzery M, Al-Baarri AN, Putri SR, Kurdianto. Real Time-Polymerase Chain Reaction (RT-PCR) sebagai alat deteksi DNA babi dalam beberapa produk non-pangan. IJH 2019; 27-29.
  • 31. Cebi N, Durak, MZ, Toker OS, Sagdic O, Arici M. An evaluation of Fourier transforms infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins. Food Chem. 2016; 90: 10-13.
  • 32. Charity ML. Jaminan produk halal di Indonesia (Halal products guarantee in Indonesia). JLI 2017; 14: 01.

Year 2021, , 216 - 223, 15.09.2021

Ismail Hutasoit , Agy Pranata Masna Wiyah , Fitri Ramadhani , Hubban Nasution

https://doi.org/10.7126/cumudj.887101

Abstract

References

  • 1. Dewi N, Mustaqimah, Roswiem AP. Identification of Gelatin in Dental Materials using Combination of Attennuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Chemometrics. IJOHR 2020; 2(1):1-15.
  • 2. Rahardian B, Hakim N, Kurniawan A. Implementation of halal product guarantee in dental health services in Islamic hospital. Int J Human and Health Sci 2019; 3(2): 54-7.
  • 3. Anusavice KJ. Philips’s science of dental materials. 12th ed. Missouri: Elsevier, 2013:5-6.
  • 4. Faaz, Adeel, Zohaib. Application of metals and alloys in dentistry. In: Khurshid Z, Sheikh Z, ed. Dental Materials (Principles and Applications). Pakistan: Paramount Books Ltd, 2018: 41.
  • 5. Chalifoux PR. Acrylic and other resin: provisional restorations. In: Aschheim KW. eds. Esthetic Dentistry: A Clinical Approach to Techniques and Materials, 3rd. St. Louis: Mosby, 2015;197-203.
  • 6. Nafis MC. The concept of halal and thayyib and its implementation in Indonesia. JHPR 2019; 2(1): 1-5. 7. Khan MI, Haleem A. Understanding “halal” and “halal certification & accreditation system”-brief review. Saudi J. Bus. Manag. Stud. 2016; 1(1): 32-42.
  • 8. Salim M. Contemporary Islamic law in Indonesia-sharia and legal pluralism. Edinburgh: Edinburgh University Press, 2015: 23-27.
  • 9. JM Muslimin. Halal Product Guarantee in Indonesia Regulation and Social Inclusion. IJEB 2019; 4: 27-29.
  • 10. Ali E, Sultana S, Hamid SBA, Hossain M, Yehya WA, et al. Gelatin controversies in food, pharmaceuticals, and personal care product: authentication methods, current status, and future challenges. University of Malaya 2017; 1-10.
  • 11. Ali ME, Kashif M., Uddin K, Hashim U., Mustafa S., Man YBC. Species authentication methods in foods and feeds: the present, past, and future of halal forensics. Food anal. Methods 2012; 5(1): 946.
  • 12. Roswiem AP, Mustaqimah DN. Identification of gelatin source in toothpaste products using combination of attenuated total reflection-fourier transform infrared (ATR-FTIR) spectroscopy and chemometrics. IJOHR 2020; 2(1): 30-37.
  • 13. Hermanto S, Sumartin LO, Fatimah W. Differentiation of bovine and porcine gelatin based on spectroscopic and electrophoretic analysis. J Food Pharm Sci 2013. 1. 68-73.
  • 14. Lee JH, Yi GY, Lee JW. Physicochemical characterization of porcine bone-derived grafting material and comparison with bovine xenografts for dental applications. JPIS 2017; 47(6): 388.
  • 15. Sudjadi, Wardani HS, Sepminarti T, Rohman A. Analysis of porcine gelatin DNA in a commercial capsule shell using Real Time polymerase chain reaction for halal authentication. IJFP 2016; 19: 2127-34.
  • 16. Rocha AJ, Monteiro-Júnior JE, Freire JEC, Sousa AJS, Fonteles CSR. Real-time PCR: the use of reference genes and essential rules required to obtain normalization data reliable to quantitative gene expression. JMBR 2015; 5(1): 45-55.
  • 17. Corona B, Lleonard R, Carpio Y, Uffo O, Martinez. Short communication, PCR detection of DNA bovine, ovine-caprine and porcine origin in feed as part a bovine spongiform encephalopathy control program. SJAR 2017; 5(3): 312-7.
  • 18. Erwanto, et al. Identification of pig DNA in product using polymerase chain reaction (PCR) for halal authentication – a review. IFRJ 2018; 25(4): 22–31.
  • 19. Loftis AD, Reeves WK. Principles of Real-Time PCR. In: Wang C, Kaltenboeck B, Freeman MD. eds. Veterinary PCR diagnostics, Basseterre: Bhentam E Books , 2012: 3-6.
  • 20. Salamah N, Erwanto Y, Martono S, Rohman A. Real-Time PCR-based detection of bovine DNA by specific targeting on cytochrome-B. JUAD 2019; 9(2): 201-08.
  • 21. Halib N, Suhaila NS, Fattah WA, et al. Islam and technological development in Malaysia’s health care: an Islam legal basis analysis of dental material used in periodontal theraphy. Mal J of So Sp. 2016; 12 (1): 88-95.
  • 22. Ifranita N, Mirghani I, Saeed ME, et al. Rapid detection of gelatin in dental materials using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR). J. Phys: Conf. Ser. 2017; 884: 1-10.
  • 23. Azrul Aziz AH, Ab Mutalib NA, Rahman RA, et al. The authentication of halal dental materials using rapid fourier transform infrared (FTIR) spectroscopy. Adv Sci. 2017; 23(5): 1-4.
  • 24. Craig’s, Powers JM, Sakaguchi RL 14th ed. Restorative dental materials. India. Mosby Elsevier. 2009; 150-204.
  • 25. Gounder R, Sathasivam P. Importance of denture adhesive in complete denture patiens-a review. IJRAMR 2016; 3 (6): 35-37.
  • 26. Manar J. Alginate as impression material. Int J Appl Dent Sci 2018; 4 (3): 300-301.
  • 27. Hatrick CD, Eakle S, Bird WF. Dental materials - clinical applications for dental assitants and dental hygienist. 3rd ed. Louis: Saunders Elsevier, 2015: 97, 106-7.
  • 28. Zarb B. Prosthodontic treatment for edentulous patient complete denture and implant-suported prosthesis. 5th ed. Amsterdam: Elsevier, 2011:442-48.
  • 29. Bobkowska BD, Medyriski D, Pryliriski M. Recent advances in tissue conditioners for prosthetic treatment: a review. Wroclaw medical university 2016; 724.
  • 30. Widayat, Agustini TW, Suzery M, Al-Baarri AN, Putri SR, Kurdianto. Real Time-Polymerase Chain Reaction (RT-PCR) sebagai alat deteksi DNA babi dalam beberapa produk non-pangan. IJH 2019; 27-29.
  • 31. Cebi N, Durak, MZ, Toker OS, Sagdic O, Arici M. An evaluation of Fourier transforms infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins. Food Chem. 2016; 90: 10-13.
  • 32. Charity ML. Jaminan produk halal di Indonesia (Halal products guarantee in Indonesia). JLI 2017; 14: 01.
There are 31 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research Articles
Authors

Ismail Hutasoit 0000-0002-5353-1994

Agy Pranata 0000-0002-9541-7209

Masna Wiyah 0000-0001-5971-6511

Fitri Ramadhani 0000-0002-0121-4014

Hubban Nasution 0000-0002-0627-3523

Publication Date September 15, 2021
Submission Date February 26, 2021
Published in Issue Year 2021

Cite

EndNote Hutasoit I, Pranata A, Wiyah M, Ramadhani F, Nasution H (September 1, 2021) REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS. Cumhuriyet Dental Journal 24 3 216–223.

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


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