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Human Osteoblast Hücre Hattı Üzerinde Qmix, Etilen Diamintetraasetik Asit ve Klorhheksidin’in Sitotoksisitesinin Değerlendirilmesi

Year 2018, , 202 - 208, 17.10.2018
https://doi.org/10.7126/cumudj.438506

Abstract

Amaç:
Bu çalışmada, QMix ™, etilendiamintetraasetik asit ve klorheksidin irrigasyon
solüsyonlarının human osteoblast hücreleri hattı üzerindeki zamana bağlı toksik
etkisi değerlendirdi.



Materyal ve metod: Human
osteoblast hücreleri, % 5 CO2 ve% 100 bağıl nem içeren bir ortamda 37ºC'de
tek tabakalı olacak şekilde kültüre edildi. Hücreler 4 saat ve 24 saat boyunca etilendiamintetraasetik
asit (EDTA), klorheksidin (CHX) ve QMix ™ 'e maruz bırakıldı. Hücre canlılığı
2,3-bis (2-metoksi-4-nitro-5-sülfenil) -5 - [(fenilamino) karbonil] -2H-tetrazolyum
hidroksit kiti (XTT) ile değerlendirildi. Human osteoblast hücrelerinin
ortalama yaşayabilirliğindeki farklılıklar istatistiksel olarak
değerlendirildi.



Bulgular:
Deney solüsyonlarındaki ve kontrol grubundaki canlı hücrelerin ortalama yüzdesi
 arasında hem 4 saatlik (p˂0,001) hem de
24 saatlik (p =0,004)  uygulamanın
sonrasında istatistiksel olarak anlamlı 
fark görüldü. Canlı hücrelerin ortalama yüzdesi, EDTA, CHX ve QMixTM
grubunda uygulama süresinin artması ile istatistiksel olarak anlamlı derecede
azaldı (p˂0,05). 4 saat süresince uygulamanın ardından CHX ‘e göre EDTA ve Qmix
istatistiksel olarak anlamı derecede daha az toksik etki gösterdi
(p˂0,05).  24 saat süresince uygulamanın
ardından ise irrigasyon solüsyonlarının toksisitesi arasında istatistiksel
olarak anlamlı fark bulunmadı (p>0,05).



Sonuç: Bu
çalışmada kullanılan irrigasyon solüsyonları human osteoblast hücre hattı
üzerinde çeşitli derecede toksik etki gösterdi. Uygulama süresinin artması kullanılan
irrigasyon solüsyonlarının  human
osteoblast hücre hattı üzerindeki toksisitesini de  arttırdı. 

References

  • 1. Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in Endodontics. Dent Clin North Am, 54(2):291-312,2010.
  • 2. Kimura Y, Wilder-Smith P, Matsumoto K. Lasers in endodontics: a review. Int Endod J, 33(3):173-185,2000.
  • 3. Desai P, Himel V. Comparative Safety of Various Intracanal Irrigation Systems. J Endod, 35(4):545-549,2009.
  • 4. Virtej A, MacKenzie CR, Raab WHM, Pfeffer K, Barthel CR. Determination of the performance of various root canal disinfection methods after in situ carriage. J Endod, 33(8):926-929,2007.
  • 5. Lui J-N, Kuah H-G, Chen N-N. Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. Journal of endodontics, 33(4):472-475,2007.
  • 6. Ozdemir HO, Buzoglu HD, Calt S, Stabholz A, Steinberg D. Effect of ethylenediaminetetraacetic acid and sodium hypochlorite irrigation on Enterococcus faecalis biofilm colonization in young and old human root canal dentin: in vitro study. Journal of endodontics, 36(5):842-846,2010.
  • 7. Soares JA, de Carvalho MAR, Santos SMC, Mendonça RMC, Ribeiro-Sobrinho AP, Brito-Júnior M, Magalhães PP, Santos MH, de Macêdo Farias L. Effectiveness of chemomechanical preparation with alternating use of sodium hypochlorite and EDTA in eliminating intracanal Enterococcus faecalis biofilm. Journal of endodontics, 36(5):894-898,2010.
  • 8. Gomes BP, Vianna ME, Zaia AA, Almeida JFA, Souza-Filho FJ, Ferraz CC. Chlorhexidine in endodontics. Brazilian dental journal, 24(2):89-102,2013.
  • 9. Souza M, Cecchin D, Farina AP, Leite CE, Cruz FF, da Cunha Pereira C, Ferraz CCR, Figueiredo JAP. Evaluation of chlorhexidine substantivity on human dentin: a chemical analysis. Journal of endodontics, 38(9):1249-1252,2012.
  • 10. Cousido MC, Carmona IT, García-Caballero L, Limeres J, Álvarez M, Diz P. In vivo substantivity of 0.12% and 0.2% chlorhexidine mouthrinses on salivary bacteria. Clinical oral investigations, 14(4):397-402,2010.
  • 11. Rôças IN, Siqueira JF. Comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. Journal of endodontics, 37(2):143-150,2011.
  • 12. Dai L, Khechen K, Khan S, Gillen B, Loushine BA, Wimmer CE, Gutmann JL, Pashley D, Tay FR. The effect of QMix, an experimental antibacterial root canal irrigant, on removal of canal wall smear layer and debris. Journal of endodontics, 37(1):80-84,2011.
  • 13. Eliot C, Hatton JF, Stewart GP, Hildebolt CF, Gillespie MJ, Gutmann JL. The effect of the irrigant QMix on removal of canal wall smear layer: an ex vivo study. Odontology, 102(2):232-240,2014.
  • 14. Elakanti S, Cherukuri G, Rao VG, Chandrasekhar V, Rao AS, Tummala M. Comparative evaluation of antimicrobial efficacy of QMix™ 2 in 1, sodium hypochlorite, and chlorhexidine against Enterococcus faecalis and Candida albicans. Journal of conservative dentistry: JCD, 18(2):128,2015.
  • 15. Gulati N, Chandra S, Aggarwal PK, Jaiswal JN, Singh M. Cytotoxicity of eugenol in sealer containing zinc-oxide. Endod Dent Traumatol, 7(4):181-185,1991.
  • 16. Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental restorative materials. J Oral Rehabil, 21(4):453-462,1994.
  • 17. Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. An in vitro study of the cytotoxicity of two root canal sealers. J Endod, 26(4):228-229,2000.
  • 18. Saw TY, Cao T, Yap AU, Lee Ng MM. Tooth slice organ culture and established cell line culture models for cytotoxicity assessment of dental materials. Toxicol In Vitro, 19(1):145-154,2005.
  • 19. Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dental Materials, 12(3):186-193,1996.
  • 20. de Sermeno RF, da Silva LA, Herrera H, Herrera H, Silva RA, Leonardo MR. Tissue damage after sodium hypochlorite extrusion during root canal treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 108(1):e46-49,2009.
  • 21. Rodrigues C, Costa-Rodrigues J, Capelas JA, Fernandes MH. Behaviour of co-cultured human osteoclastic and osteoblastic cells exposed to endodontic sealers' extracts. Clin Oral Investig, 18(2):479-488,2014.
  • 22. Khayat A. Histological observations of periradicular healing following root canal treatment. Aust Endod J, 31(3):101-105,2005.
  • 23. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clinical oral investigations, 1(4):154-162,1998.
  • 24. Chung H-J, Kim D-W. Cell proliferation and Cytotoxicity Assays. Korean Journal of Crop Science, 49(spc1):208-216,2004.
  • 25. Putnam KP, Bombick DW, Doolittle DJ. Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicology in vitro, 16(5):599-607,2002.
  • 26. Vouzara T, Koulaouzidou E, Ziouti F, Economides N. Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine. International endodontic journal,2015.
  • 27. Li YC, Kuan YH, Lee SS, Huang FM, Chang YC. Cytotoxicity and genotoxicity of chlorhexidine on macrophages in vitro. Environ Toxicol, 29(4):452-458,2014.
  • 28. Giannelli M, Chellini F, Margheri M, Tonelli P, Tani A. Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation. Toxicol In Vitro, 22(2):308-317,2008.
  • 29. AlKahtani A, Alkahtany SM, Mahmood A, Elsafadi MA, Aldahmash AM, Anil S. Cytotoxicity of QMix™ endodontic irrigating solution on human bone marrow mesenchymal stem cells. BMC oral health, 14(1):27,2014.
  • 30. Farhad Mollashahi N, Saberi E, Karkehabadi H. Evaluation of Cytotoxic Effects of Various Endodontic Irrigation Solutions on the Survival of Stem Cell of Human Apical Papilla. Iran Endod J, 11(4):293-297,2016.
  • 31. Prado M, Silva EJ, Duque TM, Zaia AA, Ferraz CC, Almeida JF, Gomes BP. Antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants. J Appl Oral Sci, 23(2):158-163,2015.
  • 32. Trevino EG, Patwardhan AN, Henry MA, Perry G, Dybdal-Hargreaves N, Hargreaves KM, Diogenes A. Effect of irrigants on the survival of human stem cells of the apical papilla in a platelet-rich plasma scaffold in human root tips. J Endod, 37(8):1109-1115,2011.
  • 33. Faria G, Celes MR, De Rossi A, Silva LA, Silva JS, Rossi MA. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured l929 fibroblasts. J Endod, 33(6):715-722,2007.

Human Osteoblast Hücre Hattı Üzerinde Qmix, Etilen Diamintetraasetik Asit ve Klorhheksidin’in Sitotoksisitesinin Değerlendirilmesi

Year 2018, , 202 - 208, 17.10.2018
https://doi.org/10.7126/cumudj.438506

Abstract

Amaç:
Bu çalışmada, QMix ™, etilendiamintetraasetik asit ve klorheksidin irrigasyon
solüsyonlarının human osteoblast hücreleri hattı üzerindeki zamana bağlı toksik
etkisi değerlendirdi.

Materyal ve metod: Human
osteoblast hücreleri, % 5 CO2 ve% 100 bağıl nem içeren bir ortamda 37ºC'de
tek tabakalı olacak şekilde kültüre edildi. Hücreler 4 saat ve 24 saat boyunca etilendiamintetraasetik
asit (EDTA), klorheksidin (CHX) ve QMix ™ 'e maruz bırakıldı. Hücre canlılığı
2,3-bis (2-metoksi-4-nitro-5-sülfenil) -5 - [(fenilamino) karbonil] -2H-tetrazolyum
hidroksit kiti (XTT) ile değerlendirildi. Human osteoblast hücrelerinin
ortalama yaşayabilirliğindeki farklılıklar istatistiksel olarak
değerlendirildi.

Bulgular:
Deney solüsyonlarındaki ve kontrol grubundaki canlı hücrelerin ortalama yüzdesi
 arasında hem 4 saatlik (p˂0,001) hem de
24 saatlik (p =0,004)  uygulamanın
sonrasında istatistiksel olarak anlamlı 
fark görüldü. Canlı hücrelerin ortalama yüzdesi, EDTA, CHX ve QMixTM
grubunda uygulama süresinin artması ile istatistiksel olarak anlamlı derecede
azaldı (p˂0,05). 4 saat süresince uygulamanın ardından CHX ‘e göre EDTA ve Qmix
istatistiksel olarak anlamı derecede daha az toksik etki gösterdi
(p˂0,05).  24 saat süresince uygulamanın
ardından ise irrigasyon solüsyonlarının toksisitesi arasında istatistiksel
olarak anlamlı fark bulunmadı (p>0,05).







Sonuç: Bu
çalışmada kullanılan irrigasyon solüsyonları human osteoblast hücre hattı
üzerinde çeşitli derecede toksik etki gösterdi. Uygulama süresinin artması kullanılan
irrigasyon solüsyonlarının human osteoblast hücre hattı üzerindeki toksisitesini
de arttırdı. 

References

  • 1. Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in Endodontics. Dent Clin North Am, 54(2):291-312,2010.
  • 2. Kimura Y, Wilder-Smith P, Matsumoto K. Lasers in endodontics: a review. Int Endod J, 33(3):173-185,2000.
  • 3. Desai P, Himel V. Comparative Safety of Various Intracanal Irrigation Systems. J Endod, 35(4):545-549,2009.
  • 4. Virtej A, MacKenzie CR, Raab WHM, Pfeffer K, Barthel CR. Determination of the performance of various root canal disinfection methods after in situ carriage. J Endod, 33(8):926-929,2007.
  • 5. Lui J-N, Kuah H-G, Chen N-N. Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. Journal of endodontics, 33(4):472-475,2007.
  • 6. Ozdemir HO, Buzoglu HD, Calt S, Stabholz A, Steinberg D. Effect of ethylenediaminetetraacetic acid and sodium hypochlorite irrigation on Enterococcus faecalis biofilm colonization in young and old human root canal dentin: in vitro study. Journal of endodontics, 36(5):842-846,2010.
  • 7. Soares JA, de Carvalho MAR, Santos SMC, Mendonça RMC, Ribeiro-Sobrinho AP, Brito-Júnior M, Magalhães PP, Santos MH, de Macêdo Farias L. Effectiveness of chemomechanical preparation with alternating use of sodium hypochlorite and EDTA in eliminating intracanal Enterococcus faecalis biofilm. Journal of endodontics, 36(5):894-898,2010.
  • 8. Gomes BP, Vianna ME, Zaia AA, Almeida JFA, Souza-Filho FJ, Ferraz CC. Chlorhexidine in endodontics. Brazilian dental journal, 24(2):89-102,2013.
  • 9. Souza M, Cecchin D, Farina AP, Leite CE, Cruz FF, da Cunha Pereira C, Ferraz CCR, Figueiredo JAP. Evaluation of chlorhexidine substantivity on human dentin: a chemical analysis. Journal of endodontics, 38(9):1249-1252,2012.
  • 10. Cousido MC, Carmona IT, García-Caballero L, Limeres J, Álvarez M, Diz P. In vivo substantivity of 0.12% and 0.2% chlorhexidine mouthrinses on salivary bacteria. Clinical oral investigations, 14(4):397-402,2010.
  • 11. Rôças IN, Siqueira JF. Comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. Journal of endodontics, 37(2):143-150,2011.
  • 12. Dai L, Khechen K, Khan S, Gillen B, Loushine BA, Wimmer CE, Gutmann JL, Pashley D, Tay FR. The effect of QMix, an experimental antibacterial root canal irrigant, on removal of canal wall smear layer and debris. Journal of endodontics, 37(1):80-84,2011.
  • 13. Eliot C, Hatton JF, Stewart GP, Hildebolt CF, Gillespie MJ, Gutmann JL. The effect of the irrigant QMix on removal of canal wall smear layer: an ex vivo study. Odontology, 102(2):232-240,2014.
  • 14. Elakanti S, Cherukuri G, Rao VG, Chandrasekhar V, Rao AS, Tummala M. Comparative evaluation of antimicrobial efficacy of QMix™ 2 in 1, sodium hypochlorite, and chlorhexidine against Enterococcus faecalis and Candida albicans. Journal of conservative dentistry: JCD, 18(2):128,2015.
  • 15. Gulati N, Chandra S, Aggarwal PK, Jaiswal JN, Singh M. Cytotoxicity of eugenol in sealer containing zinc-oxide. Endod Dent Traumatol, 7(4):181-185,1991.
  • 16. Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental restorative materials. J Oral Rehabil, 21(4):453-462,1994.
  • 17. Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. An in vitro study of the cytotoxicity of two root canal sealers. J Endod, 26(4):228-229,2000.
  • 18. Saw TY, Cao T, Yap AU, Lee Ng MM. Tooth slice organ culture and established cell line culture models for cytotoxicity assessment of dental materials. Toxicol In Vitro, 19(1):145-154,2005.
  • 19. Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dental Materials, 12(3):186-193,1996.
  • 20. de Sermeno RF, da Silva LA, Herrera H, Herrera H, Silva RA, Leonardo MR. Tissue damage after sodium hypochlorite extrusion during root canal treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 108(1):e46-49,2009.
  • 21. Rodrigues C, Costa-Rodrigues J, Capelas JA, Fernandes MH. Behaviour of co-cultured human osteoclastic and osteoblastic cells exposed to endodontic sealers' extracts. Clin Oral Investig, 18(2):479-488,2014.
  • 22. Khayat A. Histological observations of periradicular healing following root canal treatment. Aust Endod J, 31(3):101-105,2005.
  • 23. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clinical oral investigations, 1(4):154-162,1998.
  • 24. Chung H-J, Kim D-W. Cell proliferation and Cytotoxicity Assays. Korean Journal of Crop Science, 49(spc1):208-216,2004.
  • 25. Putnam KP, Bombick DW, Doolittle DJ. Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicology in vitro, 16(5):599-607,2002.
  • 26. Vouzara T, Koulaouzidou E, Ziouti F, Economides N. Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine. International endodontic journal,2015.
  • 27. Li YC, Kuan YH, Lee SS, Huang FM, Chang YC. Cytotoxicity and genotoxicity of chlorhexidine on macrophages in vitro. Environ Toxicol, 29(4):452-458,2014.
  • 28. Giannelli M, Chellini F, Margheri M, Tonelli P, Tani A. Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation. Toxicol In Vitro, 22(2):308-317,2008.
  • 29. AlKahtani A, Alkahtany SM, Mahmood A, Elsafadi MA, Aldahmash AM, Anil S. Cytotoxicity of QMix™ endodontic irrigating solution on human bone marrow mesenchymal stem cells. BMC oral health, 14(1):27,2014.
  • 30. Farhad Mollashahi N, Saberi E, Karkehabadi H. Evaluation of Cytotoxic Effects of Various Endodontic Irrigation Solutions on the Survival of Stem Cell of Human Apical Papilla. Iran Endod J, 11(4):293-297,2016.
  • 31. Prado M, Silva EJ, Duque TM, Zaia AA, Ferraz CC, Almeida JF, Gomes BP. Antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants. J Appl Oral Sci, 23(2):158-163,2015.
  • 32. Trevino EG, Patwardhan AN, Henry MA, Perry G, Dybdal-Hargreaves N, Hargreaves KM, Diogenes A. Effect of irrigants on the survival of human stem cells of the apical papilla in a platelet-rich plasma scaffold in human root tips. J Endod, 37(8):1109-1115,2011.
  • 33. Faria G, Celes MR, De Rossi A, Silva LA, Silva JS, Rossi MA. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured l929 fibroblasts. J Endod, 33(6):715-722,2007.
There are 33 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research Articles
Authors

Zeliha Uğur Aydın

Kerem Engin Akpınar

Ceylan Hepokur

Demet Altunbaş

Publication Date October 17, 2018
Submission Date June 29, 2018
Published in Issue Year 2018

Cite

EndNote Uğur Aydın Z, Akpınar KE, Hepokur C, Altunbaş D (October 1, 2018) Human Osteoblast Hücre Hattı Üzerinde Qmix, Etilen Diamintetraasetik Asit ve Klorhheksidin’in Sitotoksisitesinin Değerlendirilmesi. Cumhuriyet Dental Journal 21 3 202–208.

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