Abstract
Objective: Ozone accelerates wound healing and increases oxygen supply. The
purpose of this study was to investigate the effect of ozone therapy combined
with bone allograft on bone regeneration in rats with calvarial defects, histomorphometrically
and histopathologically.
Material-Method: Twenty four male Wistar rats were used in
this study. A 5 mm diameter critical-size defects were created in all rats
using a trephine bur. The rats were divided into 4 groups; empty defect
(control) (n=6), ozone application into the control defect (control+ ozone)
group (n=6), defect filled with allograft (Allograft) group (n=6), ozone
combined with allograft application (allograft + ozone) group (n=6). Gaseous
ozone administered at 80% density for 14 days. The animals were euthanized at
8-week.
Total bone area was measured
histomorphometrically and osteoclast and osteoblast number were analyzed
histopathologically.
Results: The
osteoblast numbers increased in allograft + ozone group compared to the
allograft group (p<0.05). The control defects showed significantly less new
bone formation than those of the allograft groups at 8 weeks (p<0.05). Total
bone area was significantly higher in allograft+ozone group than those of the
allograft group (p<0.05).
Conclusions: Within
the limitations of this study, we suggested that allograft+ozone combination
increased osteoblast number and new bone area. However, further studies are
needed to investigate the mechanisms of action of ozone on bone regeneration.
References
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- 2. Oh KC, Cha JK, Kim CS, Choi SH, Chai JK, Jung UW. The influence of perforating the autogenous block bone and the recipient bed in dogs. Part I: a radiographic analysis. Clin Oral Implants Res 2011; 22(11): 1298-302.
- 3. Reynolds MA, Bowers GM. Fate of demineralized freeze-dried bone allografts in human intrabony defects. J Periodontol 1996; 67(2): 150-157.
- 4. Salyer KE, Bardach J, Squier CA, Gendler E, Kelly M. Cranioplasty in the growing canine skull using demineralized perforated bone. Plast Reconstr Surg; 1995: 96(4):. 770-779.
- 5. Paper, A.A.P.P., Tissue Banking Of Bone Allografts Used in Periodontal Regeneration. J Periodontol 2001;72: 834-838.
- 6. Shigeyama Y, D'Errico JA, Stone R, Somerman MJ. Commercially-prepared allograft material has biological activity in vitro. J Periodontol 1995; 66(6): 478-487.
- 7. Messora MR, Nagata MJ, Fucini SE, Pola NM, Campos N, de Oliveira GC et. al. Effect of platelet-rich plasma on the healing of mandibular defects treated with fresh frozen bone allograft: a radiographic study in dogs. J Oral Implantol 2014; 40(5): 533-541.
- 8. The potential role of growth and differentiation factors in periodontal regeneration. J Periodontol, 1996;67(5): 545-553.
- 9. Fideler BM, Moore T, Li Z, Rasheed S. Effects of gamma irradiation on the human immunodeficiency virus. A study in frozen human bone-patellar ligament-bone grafts obtained from infected cadavera. J Bone Joint Surg Am 1994;76-A(7): 1032-1035.
- 10. Cornu O, Libouton X, Naets B, Godts B, Van Tomme J, Delloye C, et. a. Freeze-dried irradiated bone brittleness improves compactness in an impaction bone grafting model. Acta Orthop Scand 2004; 75(3): 309-314.
- 21. Ivanovski S, Hamlet S, Retzepi M, Wall I, Donos N. Transcriptional profiling of "guided bone regeneration" in a critical-size calvarial defect. Clin Oral Implants Res 2011; 22(4): 382-389.
- 22. Kochi G, Sato S, Fukuyama T, Morita C, Honda K, Arai Y, et. al. Analysis on the guided bone augmentation in the rat calvarium using a microfocus computerized tomography analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107(6): e42-48.
- 23. Notodihardjo FZ, Kakudo N, Kushida S, Suzuki K, Kusumoto K. Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats. J Craniomaxillofac Surg 2012; 40(3): 287-291.
- 24. Ellegaard B. Bone grafts in periodontal attachment procedures. J Clin Periodontol 1976; 3(5): 1-54.
- 25. Mellonig JT, Bowers GM, Bright RW, Lawrence JJ. Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol 1976; 47(3): 125-131.
- 26. Galgut PN, Waite IM, Brookshaw JD, Kingston CP. A 4-year controlled clinical study into the use of a ceramic hydroxylapatite implant material for the treatment of periodontal bone defects. J Clin Periodontol 1992; 19(8): p. 570-7.
- 27. Dragoo MR, Sullivan HC. A clinical and histological evaluation of autogenous iliac bone grafts in humans. I. Wound healing 2 to 8 months. J Periodontol, 1973; 44(10): 599-613.
- 28. Reynolds MA, Aichelmann-Reidy ME, Branch-Mays GL, Gunsolley JC. The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review. Ann Periodontol 2003; 8(1): 227-265.
- 29. Schmitt CM, Doering H, Schmidt T, Lutz R, Neukam FW, Schlegel KA, Histological results after maxillary sinus augmentation with Straumann(R) BoneCeramic, Bio-Oss(R), Puros(R), and autologous bone. A randomized controlled clinical trial. Clin Oral Implants Res 2013; 24(5): 576-585.
- 30. Froum SJ, Wallace SS, Elian N, Cho SC, Tarnow DP. Comparison of mineralized cancellous bone allograft (Puros) and anorganic bovine bone matrix (Bio-Oss) for sinus augmentation: histomorphometry at 26 to 32 weeks after grafting. Int J Periodontics Restorative Dent 2006; 26(6): 543-551.
Abstract
References
- 1. Jensen SS, Broggini N, Hjorting-Hansen H, Schenk R, Buser D. Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs. Clin Oral Implants Res 2006; 17(3): 237-43.
- 2. Oh KC, Cha JK, Kim CS, Choi SH, Chai JK, Jung UW. The influence of perforating the autogenous block bone and the recipient bed in dogs. Part I: a radiographic analysis. Clin Oral Implants Res 2011; 22(11): 1298-302.
- 3. Reynolds MA, Bowers GM. Fate of demineralized freeze-dried bone allografts in human intrabony defects. J Periodontol 1996; 67(2): 150-157.
- 4. Salyer KE, Bardach J, Squier CA, Gendler E, Kelly M. Cranioplasty in the growing canine skull using demineralized perforated bone. Plast Reconstr Surg; 1995: 96(4):. 770-779.
- 5. Paper, A.A.P.P., Tissue Banking Of Bone Allografts Used in Periodontal Regeneration. J Periodontol 2001;72: 834-838.
- 6. Shigeyama Y, D'Errico JA, Stone R, Somerman MJ. Commercially-prepared allograft material has biological activity in vitro. J Periodontol 1995; 66(6): 478-487.
- 7. Messora MR, Nagata MJ, Fucini SE, Pola NM, Campos N, de Oliveira GC et. al. Effect of platelet-rich plasma on the healing of mandibular defects treated with fresh frozen bone allograft: a radiographic study in dogs. J Oral Implantol 2014; 40(5): 533-541.
- 8. The potential role of growth and differentiation factors in periodontal regeneration. J Periodontol, 1996;67(5): 545-553.
- 9. Fideler BM, Moore T, Li Z, Rasheed S. Effects of gamma irradiation on the human immunodeficiency virus. A study in frozen human bone-patellar ligament-bone grafts obtained from infected cadavera. J Bone Joint Surg Am 1994;76-A(7): 1032-1035.
- 10. Cornu O, Libouton X, Naets B, Godts B, Van Tomme J, Delloye C, et. a. Freeze-dried irradiated bone brittleness improves compactness in an impaction bone grafting model. Acta Orthop Scand 2004; 75(3): 309-314.
- 21. Ivanovski S, Hamlet S, Retzepi M, Wall I, Donos N. Transcriptional profiling of "guided bone regeneration" in a critical-size calvarial defect. Clin Oral Implants Res 2011; 22(4): 382-389.
- 22. Kochi G, Sato S, Fukuyama T, Morita C, Honda K, Arai Y, et. al. Analysis on the guided bone augmentation in the rat calvarium using a microfocus computerized tomography analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107(6): e42-48.
- 23. Notodihardjo FZ, Kakudo N, Kushida S, Suzuki K, Kusumoto K. Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats. J Craniomaxillofac Surg 2012; 40(3): 287-291.
- 24. Ellegaard B. Bone grafts in periodontal attachment procedures. J Clin Periodontol 1976; 3(5): 1-54.
- 25. Mellonig JT, Bowers GM, Bright RW, Lawrence JJ. Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol 1976; 47(3): 125-131.
- 26. Galgut PN, Waite IM, Brookshaw JD, Kingston CP. A 4-year controlled clinical study into the use of a ceramic hydroxylapatite implant material for the treatment of periodontal bone defects. J Clin Periodontol 1992; 19(8): p. 570-7.
- 27. Dragoo MR, Sullivan HC. A clinical and histological evaluation of autogenous iliac bone grafts in humans. I. Wound healing 2 to 8 months. J Periodontol, 1973; 44(10): 599-613.
- 28. Reynolds MA, Aichelmann-Reidy ME, Branch-Mays GL, Gunsolley JC. The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review. Ann Periodontol 2003; 8(1): 227-265.
- 29. Schmitt CM, Doering H, Schmidt T, Lutz R, Neukam FW, Schlegel KA, Histological results after maxillary sinus augmentation with Straumann(R) BoneCeramic, Bio-Oss(R), Puros(R), and autologous bone. A randomized controlled clinical trial. Clin Oral Implants Res 2013; 24(5): 576-585.
- 30. Froum SJ, Wallace SS, Elian N, Cho SC, Tarnow DP. Comparison of mineralized cancellous bone allograft (Puros) and anorganic bovine bone matrix (Bio-Oss) for sinus augmentation: histomorphometry at 26 to 32 weeks after grafting. Int J Periodontics Restorative Dent 2006; 26(6): 543-551.
Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Original Research Articles |
| Authors | |
| Publication Date | March 24, 2017 |
| Submission Date | January 15, 2015 |
| Published in Issue | Year 2016 |
Cited By
Ozone Improved Bone Dynamic of Female Rats Using Zoledronate
Tissue Engineering Part C: Methods
https://doi.org/10.1089/ten.tec.2023.0159
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