INVESTIGATION OF THE EFFECT OF HUMIC ACID ON BONE REGENERATION IN PARIETAL DEFECTS FORMED IN RATS RATLARDA OLUŞTURULAN PARİETAL DEFEKTLERDE HUMİK ASİTİN KEMİK REJENERASYONU ÜZERİNE ETKİSİNİN ARAŞTIRILMASI

INVESTIGATION OF THE EFFECT OF HUMIC ACID ON BONE REGENERATION IN PARIETAL DEFECTS FORMED IN RATS ABSTRACT Objectives: The objective of our study is to research the effect of the local humic acid on the premature new bone amount in autogenous bone graft applications. Materials and Methods: 12-Week 24 rats of Wistar Albino species with a ranging weight of 230-250 g were used as subjects. The laboratory animals were divided into 4 groups. Those were determined as the control group, only autogenous graft-applied group, autogenous graft group applied with 100 mg/kg humic acid and autogenous graft group applied with 200 mg/kg humic acid. The bicortical bone fragment in the diameter of 5 mm at a critical size on the parietal bone of the laboratory animals was removed under physiological saline solution irrigation using an automax drill. The autogene graft obtained during the formation on the defected area was applied to the relevant experimental groups. The amount of humic acid determined for the groups of local application was applied by being mixed with the autogene graft. The laboratory animals were sacrificed on the 28th day following the procedure. Then the grafted defect area was evaluated using micro-computed tomography (Micro- CT). Results: The highest value among trabecular thickness (Tb.Th) and the ratio of the bone volume to the tissue volume (BV/ TV) was encountered in the autogenous graft group applied with 100mg/kg humic acid. The highest value in the bone volume (BV) variance was detected in the group to which only the autogenous graft was applied. A statistically significant difference was found between the control group and the only autogenous graft-applied group and the autogenous graft group applied with local 100mg/kg humic acid upon comparing the groups in pairs for bone surface area (BS) variance. A statistically significant difference was found between the control group and the other groups in the ratio of bone surface are to the bone volume (BS/BV). BS/BV value was found higher in the autogenous graft group applied with local 200mg/kg humic acid than the autogenous group applied with local 100mg/kg humic acid. Conclusions: The applied humic acid caused a decrease in the BV value. However, the positive effect of the humic acid application was observed in Tb.Th and BV/TV values, dosage increase negatively impacted the same. The dosage increase negatively affected the BS value. No positive effect of the humic acid application to BS/BV value was observed. The statistical significance between the control group and the other groups is considered to be autogenous graft.