THE EFFECT OF BISPHOSPHONATE RISEDRONATE HYDROGEL ON ALKALINE PHOSPHATASE AND OSTEOCLASTS DURING RELAPSE MOVEMENT

Tita Ratya Utari; Pinandi Pudyani; Ika Ana; Widya Asmara

doi:10.7126/cumudj.932462

EN

THE EFFECT OF BISPHOSPHONATE RISEDRONATE HYDROGEL ON ALKALINE PHOSPHATASE AND OSTEOCLASTS DURING RELAPSE MOVEMENT

Abstract

Objectives: To analyze the effect of risedronate hydrogel on enzyme alkaline phosphatase (ALP) and osteoclast/osteoblast ratio during tooth relapse movement Materials and methods: The research design is experimental with time series. The lower incisors of 75 guinea pigs are distally moved using open coil spring. The guinea pigs were divided into three groups: without risedronate (group A; n = 25); given 250 µmol/L of risedronate hydrogel (group B; n = 25), and given 500 µmol/L of risedronate hydrogel (group C; n = 25). Risedronate were applied intrasulcularly in the mesial part of the gingival sulcus every 3 days. After 14 days of stabilization, the open coil spring was removed (bisphosphonate administration was continued). The relapsed teeth and ALP levels on days 0, 3, 7, 14, and 21 were measured. The osteoclast/osteoblast ratio was measured by hematoxylin and eosin staining. ANOVA test was used to determine the difference in the three groups and their interactions with concentration and time. Results: There was a significant difference in osteoclast/osteoblast ratio on day 3 (p = 0.019, p <0.05) and 14 (p = 0.019, p <0.05): the ratio was higher in group A than in groups B and C on day 3, and the ratio was higher in group C than in groups A and B on day 14. ALP levels were significantly different on day 14 (p = 0.006, p <0.05) and 21 (p = 0.012, p <0.05). Conclusions: The intrasulcular application of bisphosphonate risedronate affected the osteoclast/osteoblast ratio and increased ALP levels.

Keywords

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Tita Ratya Utari ^*
0000-0001-6579-5260
Indonesia

Pinandi Pudyani
0000-0001-6111-1538
Indonesia

Ika Ana
0000-0001-9957-4247
Türkiye

Widya Asmara
0000-0001-6386-8892
Indonesia

Publication Date

June 30, 2022

Submission Date

May 8, 2021

Acceptance Date

April 5, 2022

Published in Issue

Year 2022 Volume: 25 Number: 2

DOI

https://doi.org/10.7126/cumudj.932462

IZ

https://izlik.org/JA58DL28ZS

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RIS / Bibtex

EndNote

Utari TR, Pudyani P, Ana I, Asmara W (June 1, 2022) THE EFFECT OF BISPHOSPHONATE RISEDRONATE HYDROGEL ON ALKALINE PHOSPHATASE AND OSTEOCLASTS DURING RELAPSE MOVEMENT. Cumhuriyet Dental Journal 25 2 103–110.

THE EFFECT OF BISPHOSPHONATE RISEDRONATE HYDROGEL ON ALKALINE PHOSPHATASE AND OSTEOCLASTS DURING RELAPSE MOVEMENT

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THE EFFECT OF BISPHOSPHONATE RISEDRONATE HYDROGEL ON ALKALINE PHOSPHATASE AND OSTEOCLASTS DURING RELAPSE MOVEMENT

Abstract

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Cited By

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