IN VITRO COMPARISON OF THE ANTIMICROBIAL EFFECTS OF DIFFERENT ROOT CANAL MEDICAMENTS ON ENTEROCOCCUS FAECALIS AND CANDIDA ALBICANS

Burçin Arıcan; Hesna Sazak Öveçoğlu

doi:10.7126/cumudj.912285

EN

IN VITRO COMPARISON OF THE ANTIMICROBIAL EFFECTS OF DIFFERENT ROOT CANAL MEDICAMENTS ON ENTEROCOCCUS FAECALIS AND CANDIDA ALBICANS

Abstract

Objectives: To evaluate the antimicrobial effects of experimental root canal medicaments on Enterococcus faecalis and Candida albicans on day 3 and day 7 using quantitative polymerase chain reaction (Q-PCR) analyses. Materials and Methods: 240 single-rooted, single-canalled human teeth were used. Root canals were shaped mechanically and embedded in acrylic blocks, then sterilized in an autoclave. The samples were divided into two groups and infected with E. faecalis and C. albicans, and then divided into subgroups (n=10); calcium hydroxide (Ca(OH)2), triple paste (TAP), double paste (DP), modified double paste (MDP), lactoferrin (Lf), negative and positive groups. At the end of 3 and 7 days, paper points containing the root canal samples were placed in empty Eppendorf tubes, and DNA was isolated. Real-time Q-PCR was applied and the data were analyzed statistically. Results: The antimicrobial effects of each medicine increased from days 3 to 7. Ca(OH)2 and TAP groups showed the similar eradication rates for E. faecalis and C. albicans on day 3 and 7 (P > 0.05). There was no significant difference between DP, MDP and Lf for the eradication of microorganisms at both experimental days (P > 0.05), except the amount of eradicated E. faecalis by DP at day 7 in which DP caught the similar percentages with TAP and Ca(OH)2 (P < 0.05). Conclusions: Experimental pastes demonstrated antimicrobial efficiancy similar to those used routinely in endodontic clinic. It is promising that lactoferrin which is a very biocompatible material can be used in different combinations as an intracanal medicament.

Keywords

Supporting Institution

Marmara University Scientific Research Projects Committee

Project Number

SAG-C-DRP 150513–0154

Thanks

The authors would like to grateful for the financial support from Marmara University. The authors also thank Prof. Dr. Tanju Kadir and Dr. Banu Can.

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Burçin Arıcan ^*
0000-0001-5757-0571
Türkiye

Hesna Sazak Öveçoğlu
0000-0003-4709-422X
Türkiye

Publication Date

September 15, 2021

Submission Date

April 9, 2021

Acceptance Date

August 5, 2021

Published in Issue

Year 2021 Volume: 24 Number: 3

DOI

https://doi.org/10.7126/cumudj.912285

IZ

https://izlik.org/JA75GN39WJ

Cite

RIS / Bibtex

EndNote

Arıcan B, Sazak Öveçoğlu H (September 1, 2021) IN VITRO COMPARISON OF THE ANTIMICROBIAL EFFECTS OF DIFFERENT ROOT CANAL MEDICAMENTS ON ENTEROCOCCUS FAECALIS AND CANDIDA ALBICANS. Cumhuriyet Dental Journal 24 3 256–265.

Öz

IN VITRO COMPARISON OF THE ANTIMICROBIAL EFFECTS OF DIFFERENT ROOT CANAL MEDICAMENTS ON ENTEROCOCCUS FAECALIS AND CANDIDA ALBICANS

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite