REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS

Ismail Hutasoit; Agy Pranata; Masna Wiyah; Fitri Ramadhani; Hubban Nasution

doi:10.7126/cumudj.887101

EN

REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS

Abstract

Objectives: This study aimed to identify porcine DNA in prosthodontic materials using Real-Time PCR. Materials and methods: Eighteen prosthodontic materials: three irreversible hydrocolloids, three elastomers, three denture adhesives, three soft denture linings, three temporary crowns, and three denture bases materials were used as the samples. It was conducted in two stages. First, extraction of dental material’s DNA and the second was Real-Time PCR analysis based on amplification curve and Ct score in yellow and green channel. Results: The sample analysis based on green channel demonstrated that all materials did not contain porcine DNA, however, 11 of 18 samples (DA-01, DA-02, DA-03, SDL-02, DB-01, DB-02, TC-03, IH-02, EM-01, EM-02, and EM-03) contained vertebrate DNA. Conclusions: All prosthodontic materials tested were not containing porcine. The halal statues of the materials were still unclear.

Keywords

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Ismail Hutasoit
0000-0002-5353-1994
Indonesia

Agy Pranata
0000-0002-9541-7209
Indonesia

Masna Wiyah
0000-0001-5971-6511
Indonesia

Fitri Ramadhani
0000-0002-0121-4014
Indonesia

Hubban Nasution ^*
0000-0002-0627-3523
Indonesia

Publication Date

September 15, 2021

Submission Date

February 26, 2021

Acceptance Date

May 17, 2021

Published in Issue

Year 2021 Volume: 24 Number: 3

DOI

https://doi.org/10.7126/cumudj.887101

IZ

https://izlik.org/JA93EK64XW

Cite

RIS / Bibtex

EndNote

Hutasoit I, Pranata A, Wiyah M, Ramadhani F, Nasution H (September 1, 2021) REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS. Cumhuriyet Dental Journal 24 3 216–223.

REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS

Abstract

Keywords

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References

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Subjects

Journal Section

Authors

Publication Date

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Acceptance Date

Published in Issue

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IZ

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