Platelet-Rich Fibrin Used as a Scaffold in Pulp Regeneration: Case Series

Ceren Çimen; Selin Şen; Elif Şenay; Tuğba Bezgin

doi:10.7126/cumudj.833807

EN

Platelet-Rich Fibrin Used as a Scaffold in Pulp Regeneration: Case Series

Abstract

Regenerative endodontic treatment (RET) is a biologically based treatment approach to provide root development and increase the prognosis of teeth. The purpose of this case series was to evaluate the clinical and radiological results of RET using platelet-rich fibrin (PRF), in 4 immature teeth with necrotic pulps. At the end of 18-24-month follow-up, no clinical symptoms were recorded. Apical closure was observed in 3 of the 4 teeth. All of the preoperative periapical lesions were healed. However, a calcified tissue formed in the middle third of the root in one case diagnosed with chronic apical periodontitis at 3-month follow-up. PRF was successful as a scaffold and can be recommended for revascularization protocol of necrotic immature teeth. However, prognosis of tooth can be attributed to many factors such as duration of pulp necrosis, pretreatment status of the periapical region and viability of living tissues.

Keywords

References

Galler K. Clinical procedures for revitalization: current knowledge and considerations. Int Endod J 2016; 49: 926-936.
Kim SG, Malek M, Sigurdsson A, Lin LM, Kahler B.. Regenerative endodontics: a comprehensive review. Int Endod J 2018; 51: 1367-1388
Geisler TM. Clinical considerations for regenerative endodontic procedures. Dent Clin North Am 2012; 56: 603-626.
American Association of Endodontics. Clinical considerations for a regenerative procedure. [PDF on Internet]. [Updated 4 January 2018; cited 30 November 2020]. Available from: www.aae.org/specialty/clinical-resources/regenerative-endodontics.
Nosrat A, Li KL, Vir K, Hicks ML, Fouad AF. Is pulp regeneration necessary for root maturation? J Endod 2013; 39: 1291-1295.
Torabinejad M, Nosrat A, Verma P, Udochukwu O. Regenerative endodontic treatment or mineral trioxide aggregate apical plug in teeth with necrotic pulps and open apices: a systematic review and meta-analysis. J Endod 2017; 43: 1806-1820.
Digka A, Sakka D, Lyroudia K. Histological assessment of human regenerative endodontic procedures (REP) of immature permanent teeth with necrotic pulp/apical periodontitis: A systematic review. Aust Endod J 2020; 46: 140-153.
Bezgin T, Yılmaz AD, Celik BN, Kolsuz ME, Sönmez H. Efficacy of platelet-rich plasma as a scaffold in regenerative endodontic treatment. J Endod 2015; 41: 36-44.

Bakhtiar H, Esmaeili S, Fakhr Tabatabayi S, Ellini MR, Nekoofar MH, Dummer PM. Second-generation platelet concentrate (platelet-rich fibrin) as a scaffold in regenerative endodontics: a case series. J Endod 2017; 43: 401-408.
Chai J, Jin R, Yuan G, Kanter V, Miron RJ, Zhang Y. Effect of Liquid Platelet-rich Fibrin and Platelet-rich Plasma on the Regenerative Potential of Dental Pulp Cells Cultured under Inflammatory Conditions: A Comparative Analysis. J Endod 2019;45:1000-1008.
Ulusoy AT, Turedi I, Cimen M, Cehreli ZC. Evaluation of Blood Clot, Platelet-rich Plasma, Platelet-rich Fibrin, and Platelet Pellet as Scaffolds in Regenerative Endodontic Treatment: A Prospective Randomized Trial. J Endod 2019; 45: 560-566.
Arango-Gómez E, Nino-Barrera JL, Nino G, Jordan F, Sossa-Rojas H. Pulp revascularization with and without platelet-rich plasma in two anterior teeth with horizontal radicular fractures: a case report. Restor Dent Endod 2019; 44.
Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, Gogly B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol and Endod 2006; 101: e37-e44.
Nagaveni NB, Poornima P, Joshi JS, Pathak S, Nandini DB. Revascularization of immature, nonvital permanent tooth using platelet-rich fibrin in children. Pediatr Dent 2015; 37: 1E-6E.
Ray HL Jr, Marcelino J, Braga R, Horwat R, Lisien M, Khaliq S. Long-term follow up of revascularization using platelet-rich fibrin. Dent Traumatol 2016; 32: 80-84.
Lv H, Chen Y, Cai Z, Lei L, Zhang M, Zhou R, Huang X. The efficacy of platelet-rich fibrin as a scaffold in regenerative endodontic treatment: a retrospective controlled cohort study. BMC Oral Health 2018; 18: 1-8.
Kandemir Demirci G, Güneri P, Çalışkan M. Regenerative Endodontic Therapy with Platelet Rich Fibrin: Case Series. J Clin Pediatr Dent 2020; 44: 15-19.
Hotwani K, Sharma K. Platelet rich fibrin-a novel acumen into regenerative endodontic therapy. Restor Dent Endod 2014; 39: 1-6.
Fouad AF, Verma P. Healing after regenerative procedures with and without pulpal infection. J Endod 2014; 40: S58-S64.
Verma P, Nosrat A, Kim JR, Price JB, Wang P, Bair E, Xu HH, Fouad AF. Effect of residual bacteria on the outcome of pulp regeneration in vivo. J Dent Res 2017; 96: 100-106.
Cameron R, Claudia E, Ping W, Erin S, Ruparel NB. Effect of a residual biofilm on release of transforming growth factor β1 from dentin. J Endod 2019; 45: 1119-1125.
Meschi N, Hilkens P,Van Gorp G, Strijbos O, Mavridou A, Cadenas de Llano Perula M, Lambrichts I, Verbeken E, Lambrechts P. Regenerative endodontic procedures posttrauma: Immunohistologic analysis of a retrospective series of failed cases. J Endod 2019; 45: 427-434.
Cehreli ZC, Isbitiren B, Sara S, Erbas G.. Regenerative endodontic treatment (revascularization) of immature necrotic molars medicated with calcium hydroxide: a case series. J Endod 2011; 37: 1327-1330.
Rôças IN, Siqueira Jr JF. Detection of antibiotic resistance genes in samples from acute and chronic endodontic infections and after treatment. Arch Oral Biol 2013; 58: 1123-1128.
Flake NM, Gibbs JL, Diogenes A, Hargreaves KM, Khan AA. A standardized novel method to measure radiographic root changes after endodontic therapy in immature teeth. J Endod 2014; 40: 46-50.
Jadhav GR, Shah N, Logani A. Comparative outcome of revascularization in bilateral, non-vital, immature maxillary anterior teeth supplemented with or without platelet rich plasma: A case series. J Conserv Dent 2013; 16: 568.
Murray PE. Platelet-rich plasma and platelet-rich fibrin can induce apical closure more frequently than blood-clot revascularization for the regeneration of immature permanent teeth: a meta-analysis of clinical efficacy. Front Bioeng Biotechnol 2018; 6: 139.
Nosrat A, Homayounfar N, Oloomi K. Drawbacks and unfavorable outcomes of regenerative endodontic treatments of necrotic immature teeth: a literature review and report of a case. J Endod 2012; 38: 1428-1434.
Kahler B, Mistry S, Moule A, Ringsmuth AK, Case P, Thomson A, Holcombe T. Revascularization outcomes: a prospective analysis of 16 consecutive cases. J Endod 2014; 40: 333-338.
Lin LM, Kim SG, Martin G, Kahler B. Continued root maturation despite persistent apical periodontitis of immature permanent teeth after failed regenerative endodontic therapy. Aust Endod J 2018; 44: 292-299.
Song M, Cao Y, Shin SJ, Shon WJ, Chugal N, Kim RH, Kim E, Kang MK. Revascularization-associated intracanal calcification: assessment of prevalence and contributing factors. J Endod 2017; 43: 2025-2033.
Bonson S, Jeansonne BG, Lallier TE. Root-end filling materials alter fibroblast differentiation. J Dent Res 2004; 83: 408-413.

Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Case Report

Authors

Ceren Çimen ^*
0000-0002-0641-0831
Türkiye

Selin Şen
0000-0002-3301-9651
Türkiye

Elif Şenay
0000-0002-9150-4997
Türkiye

Tuğba Bezgin
0000-0002-3025-308X
Türkiye

Publication Date

February 24, 2021

Submission Date

November 30, 2020

Acceptance Date

January 12, 2021

Published in Issue

Year 1970 Volume: 24 Number: 1

DOI

https://doi.org/10.7126/cumudj.833807

IZ

https://izlik.org/JA39BW64LT

Cite

RIS / Bibtex

EndNote

Çimen C, Şen S, Şenay E, Bezgin T (February 1, 2021) Platelet-Rich Fibrin Used as a Scaffold in Pulp Regeneration: Case Series. Cumhuriyet Dental Journal 24 1 113–120.

Cited By

CHARACTERIZATION OF A NOVEL CROSS LINKED PRF UNDER COMPRESSION INTENDED FOR PULP TISSUE ENGINEERING

GLOBAL JOURNAL FOR RESEARCH ANALYSIS

https://doi.org/10.36106/gjra/2601787

Öz

Platelet-Rich Fibrin Used as a Scaffold in Pulp Regeneration: Case Series

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

CHARACTERIZATION OF A NOVEL CROSS LINKED PRF UNDER COMPRESSION INTENDED FOR PULP TISSUE ENGINEERING