EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM

Sevim Atılan Yavuz; Derya Surmelioglu

doi:10.7126/cumudj.792699

EN

EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM

Abstract

ABSTRACT Objectives: The purpose of this study was to explore the cytotoxic effects of five different universal bonding agents on mouse fibroblast cell lines (L929). Materials and Methods: Five different widely used universal adhesive systems were chosen that have different contents, pH levels, and polymerization methods. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Germany, and ACEA Biosciences, USA) was used for cytotoxic evaluation of light-cured polymerized G-Premio Bond (GC Europe, Belgium), Prime&Bond Universal (Dentsply Sirona, USA), Universal Bond Quick (Kuraray, USA), Single Bond Universal (3M ESPE, USA) and self-cured polymerized Tokuyama Universal Bond (Tokuyama, USA) experimental groups. L929 were cultured in Dulbecco’s modified Eagle’s medium and supplemented with 10% fetal bovine serum and 1 % antibiotics. The assay was performed E-plate-16 and monitored every 15 min for 72 h. Statistical analysis was performed using ANOVA and Tukey’s posthoc tests. Results: All tested universal adhesive systems showed a statistically significant difference in cytotoxicity values in different time periods (p<0.05). Among the groups compared, G-Premio Bond showed the least cytotoxic effect; and Tokuyama Universal Bond showed the most cytotoxic effect. Different times of all universal adhesive systems significantly increased the count of viable cells compared to the control group (p<0.05). Conclusions: In dentistry, universal adhesive systems can be observed cytotoxic effects to live cells. The evaluation of cytotoxicity with xCELLigence device is a reliable method and should be supported by new studies on this subject.

Keywords

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Sevim Atılan Yavuz
0000-0002-6192-4931
Türkiye

Derya Surmelioglu ^*
0000-0002-6034-3131
Türkiye

Publication Date

December 31, 2020

Submission Date

September 9, 2020

Acceptance Date

November 25, 2020

Published in Issue

Year 2020 Volume: 23 Number: 4

DOI

https://doi.org/10.7126/cumudj.792699

IZ

https://izlik.org/JA99DP53PZ

Cite

RIS / Bibtex

APA

Atılan Yavuz, S., & Surmelioglu, D. (2020). EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM. Cumhuriyet Dental Journal, 23(4), 371-381. https://doi.org/10.7126/cumudj.792699

AMA

1.Atılan Yavuz S, Surmelioglu D. EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM. Cumhuriyet Dent J. 2020;23(4):371-381. doi:10.7126/cumudj.792699

Chicago

Atılan Yavuz, Sevim, and Derya Surmelioglu. 2020. “EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM”. Cumhuriyet Dental Journal 23 (4): 371-81. https://doi.org/10.7126/cumudj.792699.

EndNote

Atılan Yavuz S, Surmelioglu D (December 1, 2020) EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM. Cumhuriyet Dental Journal 23 4 371–381.

IEEE

[1]S. Atılan Yavuz and D. Surmelioglu, “EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM”, Cumhuriyet Dent J, vol. 23, no. 4, pp. 371–381, Dec. 2020, doi: 10.7126/cumudj.792699.

ISNAD

Atılan Yavuz, Sevim - Surmelioglu, Derya. “EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM”. Cumhuriyet Dental Journal 23/4 (December 1, 2020): 371-381. https://doi.org/10.7126/cumudj.792699.

JAMA

1.Atılan Yavuz S, Surmelioglu D. EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM. Cumhuriyet Dent J. 2020;23:371–381.

MLA

Atılan Yavuz, Sevim, and Derya Surmelioglu. “EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM”. Cumhuriyet Dental Journal, vol. 23, no. 4, Dec. 2020, pp. 371-8, doi:10.7126/cumudj.792699.

Vancouver

1.Sevim Atılan Yavuz, Derya Surmelioglu. EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM. Cumhuriyet Dent J. 2020 Dec. 1;23(4):371-8. doi:10.7126/cumudj.792699

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