Research Article

FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL

Volume: 23 Number: 4 December 31, 2020
  • Saka Winias *
  • Desiana Radithia
  • Andari Sarasati
  • Bagus Soebadi
  • Fatma Mahdani
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FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL

Abstract

Objective: Neuropathic pain and treatment side effects decreasing quality of life, reducing productivity, and high costs due to long duration of treatment. Regenerative medicine is a new and effective alternative treatment for neuropathic pain, one of which is Platelet-Rich Plasma (PRP) therapy. The objective if this study is to prove that there are differences in neuroregeneration post-crush injury after FD-PRP administration. Materials and Methods: Neuropathic pain model was made with crushing method, by compressing the infraorbital nerve using the artery clamp for 15 seconds. Rats divided into six groups. Each group was observed on day 14 (A, B, C) and 21 (A', B', C') to observe the macrophages, lymphocytes, and Schwann cells with Hematoxylin Eosin staining seen on horizontal plane of the infraorbital nerve with 400x magnification. Face grooming observations were performed on day 0 (A0, B0, C0), day 7 (A7, B7, C7), day 14 (A14, B14, C14), day 21 (A21, B21, C21). Results: There were significant differences in face grooming frequencies between groups on day 0 (p = 0.002). ANOVA Same Subject test on A`, B`, and C` revealed significant differences in macrophages and lymphocytes with score 0.02 (p <0.05) and 0.013 (p<0.05), respectively. There were significant differences between group A' and B' and between B' and C' (p>0.05). Conclusions: FD-PRP promotes nerve regeneration in axonotmesis, which was characterized by a decrease in face grooming frequency on day 7 and an increase in the number of lymphocytes, macrophages and Schwann cells on day 21.

Keywords

Supporting Institution

Faculty of Dental Medicine Universitas Airlangga - Surabaya Indonesia

Project Number

grant numbers 1408/UN3/2019

Thanks

The authors would thank to the Stem Cell Center and Tissue Bank of Dr. Soetomo Hospital, Surabaya for its support.

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Desiana Radithia
0000-0003-2031-6283
Indonesia

Andari Sarasati
0000-0003-4750-4620
Indonesia

Bagus Soebadi
0000-0001-8071-6639
Indonesia

Fatma Mahdani
0000-0001-6919-418X
Indonesia

Publication Date

December 31, 2020

Submission Date

April 20, 2020

Acceptance Date

August 26, 2020

Published in Issue

Year 2020 Volume: 23 Number: 4

DOI

https://doi.org/10.7126/cumudj.701264

IZ

https://izlik.org/JA55PC32CT

Cite

RIS / Bibtex
APA
Winias, S., Radithia, D., Sarasati, A., Soebadi, B., & Mahdani, F. (2020). FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL. Cumhuriyet Dental Journal, 23(4), 254-261. https://doi.org/10.7126/cumudj.701264
AMA
1.Winias S, Radithia D, Sarasati A, Soebadi B, Mahdani F. FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL. Cumhuriyet Dent J. 2020;23(4):254-261. doi:10.7126/cumudj.701264
Chicago
Winias, Saka, Desiana Radithia, Andari Sarasati, Bagus Soebadi, and Fatma Mahdani. 2020. “FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL”. Cumhuriyet Dental Journal 23 (4): 254-61. https://doi.org/10.7126/cumudj.701264.
EndNote
Winias S, Radithia D, Sarasati A, Soebadi B, Mahdani F (December 1, 2020) FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL. Cumhuriyet Dental Journal 23 4 254–261.
IEEE
[1]S. Winias, D. Radithia, A. Sarasati, B. Soebadi, and F. Mahdani, “FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL”, Cumhuriyet Dent J, vol. 23, no. 4, pp. 254–261, Dec. 2020, doi: 10.7126/cumudj.701264.
ISNAD
Winias, Saka - Radithia, Desiana - Sarasati, Andari - Soebadi, Bagus - Mahdani, Fatma. “FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL”. Cumhuriyet Dental Journal 23/4 (December 1, 2020): 254-261. https://doi.org/10.7126/cumudj.701264.
JAMA
1.Winias S, Radithia D, Sarasati A, Soebadi B, Mahdani F. FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL. Cumhuriyet Dent J. 2020;23:254–261.
MLA
Winias, Saka, et al. “FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL”. Cumhuriyet Dental Journal, vol. 23, no. 4, Dec. 2020, pp. 254-61, doi:10.7126/cumudj.701264.
Vancouver
1.Saka Winias, Desiana Radithia, Andari Sarasati, Bagus Soebadi, Fatma Mahdani. FREEZE-DRIED PLATELET-RICH PLASMA PROMOTES TRIGEMINAL NEUROPATHIC PAIN RELIEF IN A RAT MODEL. Cumhuriyet Dent J. 2020 Dec. 1;23(4):254-61. doi:10.7126/cumudj.701264

Cited By

Using Platelet-Rich Plasma Against Trigeminal Neuralgia: Is It an Alternative? A Mini-Review

SSRN Electronic Journal

https://doi.org/10.2139/ssrn.4450203

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