Research Article

Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques

Volume: 22 Number: 4 December 29, 2019
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Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques

Abstract

Objectives: Chitosan (poly-1,4-D-glucosamine) become popular as bone graft material. It has a high biocompatibility and biodegradability, non-toxic, bacteriostatic, and has good adsorption properties. It also enhances the proliferation and differentiation of the osteoblast cell cultures. However, chitosan also has a weakness for cell adhesion and the lack of sufficient bone formation capabilities. To improve it, we tried to develop a chitosan scaffolds that combine with carbonate apatite (CA), which has excellent biocompatibility properties of the tissues of the human body. 
Purpose: to make the carbonate apatite-chitosan scaffolds (CA-ChSs) and evaluate CA-ChSs from the standpoint of cell proliferation using mesenchymal stem cells (MSCs). 
Materials and Methods: Porous chitosan matrix was made by using the lyophilization technique. ChSs containing chitosan powder was made by the following procedure. 100, 150, 200, and 400 mg of chitosan powder was dissolved in 5 ml of 2% acetic acid at room temperature. To make CA-ChSs, ChSs containing 200 mg of chitosan powder was chosen. Ultraviolet radiation was then performed for 2 hours. CA-ChSs structure was observed by a scanning electron microscope (SEM). Proliferation of MSCs in CA-ChSs evaluated at days 1, 3, 5 and 7.
Results:This study demonstrated that CA-ChSs containing 200 mg of chitosan powder and 50 mg CA has a three-dimensional structure that is porous and attachment powder CA in the pores and absorbance values were increased from the examination day 1 until day 7. 
Conclusion:Based on these results, it is expected CA-ChSs may be candidates for bone graft material in tissue engineering techniques.

Keywords

References

  1. Debnath, T. Ghosh, S. Potlapuvu, US. Kona, L, Kamaraju, SR. Sarkar, S. Gaddam, S. Chelluri, LK. Proliferation and differentiation potential of human adipose-derived stem cells grown on cytosan hydrogel. Plos One. 2015; 10(3): e0120803.

Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Maretaningtias Dwi Ariani
0000-0002-7473-2015
Indonesia

Publication Date

December 29, 2019

Submission Date

July 23, 2019

Acceptance Date

November 4, 2019

Published in Issue

Year 1970 Volume: 22 Number: 4

DOI

https://doi.org/10.7126/cumudj.595455

IZ

https://izlik.org/JA34HS66UX

Cite

RIS / Bibtex
EndNote
Oki AS, Ariani MD (December 1, 2019) Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques. Cumhuriyet Dental Journal 22 4 412–418.

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Textile Research Journal

https://doi.org/10.1177/0040517521994884

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


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