Antibacterial activity of calcium hydroxide combined with triple antibiotic paste against enterococcus faecalis; an ın vitro study

Adem Kuşgöz; Erhan Özcan; İpek Arslan; Melek İnci

doi:10.7126/cdj.2012.1571

EN TR

Antibacterial activity of calcium hydroxide combined with triple antibiotic paste against enterococcus faecalis; an ın vitro study

Abstract

Objectives: The aim of the present in vitro study was to investigate the antibacterial efficacy of calcium hydroxide mixed with triple antibiotic paste at various rates.

Materials and Methods: Triple antibiotic paste (TAP; metronidizol, minocycline, ciprofloxacin) and calcium hydroxide powder was mixed in different proportions and tested for antibacterial activity against E. faecalis (ATCC 29212)using the agar diffusion method. The samples were divided into 10 different groups;Group 1: 100% Ca(OH)₂, Group 2: 99,5% Ca(OH)₂ + 0.5% TAP, Group 3: 99% Ca(OH)₂ + 1%TAP, Group 4: 97,5% Ca(OH)₂ + 2,5% TAP, Group 5: 95% Ca(OH)₂ + 5%TAP, Group 6: 90% Ca(OH)₂ + 10%TAP, Group 7: 75% Ca(OH)₂ + 25% TAP, Group 8: 50% Ca(OH)₂ + 50% TAP, Group 9: 25% Ca(OH)₂ + 75% TAP, Group 10: %100 TAP. Standard holes in the cultivated agar plates were filled with one of the samples and the zones of microbial inhibition were measured after incubation period (48 hours). The experiment was performed 8 times with each medicament.

Results: Group 1 had no effect against E.faecalis. Group 10 demonstrated high antibacterial activity than any other groups in this study. The antibacterial effects of the groups could be ranked from strongest to weakest as follows: Group 10, 9, 8, 7, 6, 5, 4, 3, 2, 1.

Conclusions: Adding the triple antibiotic paste increased he antibacterial efficacy of calcium hydroxide. In addition, the antibacterial effect of calcium hydroxide also increased with higher proportion of TAP.

Keywords

Antibacterial activity, Calcium Hydroxide, Enterecoccus Faecalis, Triple Antibiotic Paste

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Details

Primary Language

English

Subjects

-

Journal Section

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Authors

Adem Kuşgöz

Erhan Özcan

İpek Arslan

Melek İnci

Publication Date

January 31, 2013

Submission Date

May 23, 2012

Acceptance Date

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Year 1970 Volume: 16 Number: 1

DOI

https://doi.org/10.7126/cdj.2012.1571

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https://izlik.org/JA75ZL89AU

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RIS / Bibtex

EndNote

Kuşgöz A, Özcan E, Arslan İ, İnci M (January 1, 2013) Antibacterial activity of calcium hydroxide combined with triple antibiotic paste against enterococcus faecalis; an ın vitro study. Cumhuriyet Dental Journal 16 1 25–30.

Cited By

Assessing Cell Viability: Comparative Analysis of Calcium Hydroxide, Triple Antibiotic Paste, and Their Synergistic Impact on human Dental Pulp Stem Cells

European Journal of General Dentistry

https://doi.org/10.1055/s-0044-1788041

Antibacterial activity of calcium hydroxide combined with triple antibiotic paste against enterococcus faecalis; an ın vitro study

Abstract

Keywords

-

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Assessing Cell Viability: Comparative Analysis of Calcium Hydroxide, Triple Antibiotic Paste, and Their Synergistic Impact on human Dental Pulp Stem Cells