Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats

Turgay Peyami Hocaoğlu; Sadık Gençoğlan; Murat Arslan; M. Emre Benlidayı; Mehmet Kürkçü

doi:10.7126/cumudj.475498

TR EN

Ratlarda Deneysel Olarak Oluşturulan Kritik boyutlu kemik defektlerine uygulanan sığır kaynaklı deminarelize kemik greftininin kemik iyiyleşmesine olan etkisinin otojen,allojenik ve sentetik greftlerle karşılaştırılmasının histomorfometrik olarak incelenmesi

Öz

Amaç: Kemik dokusu iyileşme özelliğine (rejenerasyon) sahiptir ve yaralanan kemik dokusu şekil ve fonksiyonunu yeniden kazanabilmektedir. Fakat yaralanmanın boyutu büyük olduğu zaman iyileşme sınırlı kalabilmektedir. Kritik boyutlu kemik defekti; kemik dokusunda, canlının yaşamı boyunca, şekil ve fonksiyon olarak, kendiliğinden tamamen iyileşmesinin mümkün olmayacağı boyuttaki defekt anlamına gelir. Kritik kemik defektlerinde tedavi için otojen greft uygulaması altın standart olarak kabul edilir. Otojen kemik greftinin bazı dezavantajları nedeniyle araştırmacılar çalışmalarını farklı greft materyalleri üzerinde yoğunlaştırmışlardır. Bu çalışmalar neticesinde Ksenogreft, Allogreft ve sentetik greft materyalleri gibi seçenekler ortaya çıkmıştır. Çalışmamızın amacı 5mm çapında kritik boyutlu kemik defektlerinde sığır kaynaklı demineralize kemik grefti uygulamasının kemik iyileşmesine etkisi ile aynı çaptaki defektlere otojenik, allojenik ve sentetik greft materyali uygulandığı zaman elde edilen iyileşmelerin histomorfometrik olarak incelenmesidir. Gereç ve Yöntemler: Deney hayvanları her grup 8 deney hayvanından oluşan 4 gruba ayrıldı. Kontrol grubunda mandibulada defekt oluşturulduktan sonra defekt sığır kaynaklı kemik grefti (Integros Bone Plus XS Adana/Türkiye) ile dolduruldu. Daha sonraki deney gruplarında; I. grupta oluşturulan kritik boyutlu defekte trefin frezle çıkartılan otojen kemik tekrar konuldu. II. grupta oluşturulan kritik boyutlu defekte insan kaynaklı kemik grefti (Korea Bone Bank (KBB) Gasandong Keumcheongu Seoul/Korea) uygulandı. III. grupta oluşturulan kritik boyutlu kemik defektine ise sentetik kemik grefti grubunda yer alan β-trikalsiyum fosfat (Cerasorb North Caroline/USA) uygulandı. 28 gün sonra ratlar öldürüldü.Her grup sakrifiye edilerek histomorfometrik incelemeye alındı. Sonuç: Farklı greft materyallerinin 28. günde yeni kemik oluşumuna olan etkisinin histomorfometrik olarak incelendiğinde otojen kemik grefti, allogreft ve sığır kaynaklı kemik grefti uygulanan gruplar arasında yeni oluşan kemik hacmi bakımından anlamlı bir fark bulunmazken, sentetik kemik grefti uygulanan grupla aralarındaki fark anlamlı bulunmuştur. Otojen kemik grefti günümüzde hala altın standart olarak kabul edilmesine rağmen, elde edilmesi ve uygulanmasındaki zorluklar nedeniyle çalışmamızda kullandığımız sığır kaynaklı kemik greftinin otojen kemik greftine alternatif olarak güvenilir ve etkili biçimde kullanılabileceği belirlenmiştir.

Anahtar Kelimeler

dental greft,otojen greft,allojenik greft,ksenogreft

Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats

Abstract

Aim: Bone tissue has the ability to heal itself (regeneration) and may restore its morphology and function when injured. However, healing may be limited in the case of large wounds. A “critical-size defect” is an intraosseous wound in a particular bone and species of animal that will not heal spontaneously morphologically and functionally during the lifetime of the animal. Autogenous bone grafts have been regarded as “gold standard” for treatment of critical-size bone defects. Known drawbacks of autogenous bone graft have led to research efforts focusing on different graft materials and resulted in several alternative substitutes including xenografts, allografts and synthetic graft materials. The aim of the present study was to perform a histomorphometric study to investigate the effect of bovine demineralized bone graft on bone healing in comparison to autogenous, allogeneic and synthetic graft materials when applied into critical size bone defects with a diameter of 5 mm. Materials and methods: Experimental animals were divided into 4 groups, each having 8 rats. In the control group, a mandibular defect was created and then filled with a bovine graft (Integros Bone Plus XS Adana/Turkey). In the experimental groups, autogenous bone was reinserted into the critical-size defect which was created using a trephine bur in Group I (autogenous group) and Group II received a human graft (Korea Bone Bank (KBB) Gasandong Keumcheongu Seoul/South Korea) to fill the critical-size defect. For Group III, a synthetic bone graft β-tricalcium phosphate (Cerasorb North Carolina/USA) was applied on the critical-size bone defect. Specimens were obtained for histomorphometric examination and rats were sacrificed on day 28. Results: Histomorphometric examination performed on day 28 to evaluate the relative effects of different graft materials on new bone formation showed no significant difference in the volume of newly formed bone between groups receiving autogenous bone graft, allograft and bovine xenograft but a significant difference was observed versus synthetic bone graft group. Conclusion: While autogenous bone graft is currently regarded as the gold standard for bone regeneration, the difficulties in harvesting and application of autografts limit their use. Our results demonstrate that bovine bone graft may be used as a safe and effective alternative to autogenous bone graft.

Keywords

dental graft,autogenous graft,allogeneic graft,xenograft

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Turgay Peyami Hocaoğlu ^*
0000-0002-0281-8928
Türkiye

Sadık Gençoğlan
0000-0001-6902-6653
Türkiye

Murat Arslan
0000-0003-1408-9168
Türkiye

M. Emre Benlidayı

Mehmet Kürkçü

Publication Date

December 30, 2018

Submission Date

October 28, 2018

Acceptance Date

December 10, 2018

Published in Issue

Year 2018 Volume: 21 Number: 4

DOI

https://doi.org/10.7126/cumudj.475498

IZ

https://izlik.org/JA47XL75ZT

Cite

RIS / Bibtex

EndNote

Hocaoğlu TP, Gençoğlan S, Arslan M, Benlidayı ME, Kürkçü M (December 1, 2018) Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats. Cumhuriyet Dental Journal 21 4 387–395.