Investigation of cytotoxic effects of Eugenia caryophyllus (Clove)
Abstract
Objectives: Eugenia caryophyllus has been used as a traditional medicinal herb for thousands of years. It is used as an analgesic for tooth and toothache with the effect of eugenol substance included in clove. Clove is used at toothpaste and some mouthwash. The aim of this study was determined of in vitro cytotoxic effects Eugenia caryophyllus on fibroblast cells.
Materials and Methods: Cytotoxic activity of Eugenia caryophyllus was evaluated using XTT assay.
Results: In the present study, toxicity of clove that is used as a pain reliever in dentistry has examined on L929 cells. Eugenia caryophyllus were incubated at 24 and 48 hours. The IC50 values of Eugenia caryophyllus (Clove) were found 57,92 ± 0,98 µg/mL in 24 h, 37,97 ± 0,74 µg/mL in 48h.
Conclusions: The acceptable level of cytotoxicity associated with Eugenia caryophyllus requires further evaluation to be usedKeywords
References
- 1. Toroğlu S. ve Çenet M. KSÜ Fen ve Mühendislik Dergisi. 2006; 9(2):12-20.
- 2. Hammer KA, Carson CF, Riley TV. Journal of Applied Microbiology.1999;86: 985-990.
- 3. Cortés-Rojas DF, Souza CRF, Oliveira WP. Clove (Syzygium aromaticum): a precious spice. Asian Pac J Trop Biomed. 2014;4(2): 90-96.
- 4. Cansian RL, Vanin AB, Orlando T, Piazza SP, Puton BM, Cardoso RI, Gonçalves IL, Honaiser TC, Paroul N, Oliveira D. Toxicity of clove essential oil and its ester eugenyl acetate against Artemia salina. Braz J Biol. 2017; 77(1):155-161. doi: 10.1590/1519-6984.12215.
- 5. Mahmoud H, Mina KA, Hassan R. Analgesic effect of clove essential oil in mice. Avi J Phytomed. 2011;1:1-6.
- 6. Gezegen H, Hepokur C, Tutar U, Ceylan M. Synthesis and Biological Evaluation of Novel 1-(4-(Hydroxy(1-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)phenyl)-3-phenylurea Derivatives. Chemistry Biodiversity. 2017;14 (10):doi: 10.1002/cbdv.201700223.
- 7. Heinrich M, Bremner P. Ethanobotany and ethanopharmacy-their role for anticancer drug development. Curr Drug Targets. 2006; 7: 239-245.
- 8. Thirumal M, Kishore G, Prithika R, Das S, Nithya G. In vitro anticancer activity of Tecoma stans (L) ethanolic leaf extract on human breast cancer. Journal of Pharmacognosy and Phytochemistry. 2016; 5(4): 331-334.
Details
Primary Language
English
Subjects
Health Care Administration
Journal Section
Research Article
Authors
Ceylan Hepokur
*
Türkiye
Publication Date
October 17, 2018
Submission Date
July 17, 2018
Acceptance Date
July 26, 2018
Published in Issue
Year 1970 Volume: 21 Number: 3