Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study

Suresh Shenvi; Sonal B Joshi; Kavalipurapu Venkata Teja

doi:10.7126/cumudj.1968792

EN

Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study

Abstract

Aim This study aimed to evaluate and compare the effects of N-Acetylcysteine (NAC) and Ethylene Diamine tetraacetic acid (EDTA) as final irrigation solutions on the push-out bond strength of AH Plus and BioRoot RCS. Methods Ninety-two extracted single-rooted mandibular premolar teeth were prepared and divided into two groups (n = 46) at random according to the final irrigant (20% NAC or 17% EDTA). Four subgroups emerged by further dividing each group (n = 23) based on sealer employed (AH Plus or BioRoot RCS). The single-cone method was employed to obturate the canals, which were then kept for fifteen days. A Universal Testing Machine was employed for evaluating the push-out bond strength at coronal, middle, and apical thirds. Three-way mixed-model Analysis of variance (ANOVA) was utilized for assessing the data at a 5% significance level. Results AH Plus exhibited higher bond strength with EDTA, whereas BioRoot RCS performed better with NAC in coronal and middle zones (P < 0.0001). Conclusion EDTA optimizes AH Plus adhesion, while NAC enhances BioRoot RCS sealer performance, suggesting NAC as a potential alternative irrigant for improved sealer bonding.

Keywords

Supporting Institution

KLE ACADEMY OF HIGHER EDUCATION AND RESEARCH

Project Number

KAHER/EC/21-22/D-290721002/11

Ethical Statement

Dear Research Scholar, The KAHER Ethics Committee on Human Subjects for Ph.D. Research Project met on the 7th and 8th June, 2021 to consider your application for approval of the research project: “An in-vitro evaluation of N-Acetylcysteine as Root canal irrigant.” As per the specifications submitted by you, the ethical committee has reviewed the reasons and has agreed to waive off ethical approval as: It doesn’t involve human participants directly. Is involving decoded identity of participants.

Thanks

(Dr. Sheetal H. Harakuni) Member-Secretary Ethical Committee (Human) for Ph.D. Research KAHER, Belagavi. (Dr. B.V. Kotimatot) Chairman Ethical Committee (Human) for Ph.D. Research KAHER, Belagavi.

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Details

Primary Language

English

Subjects

Endodontics

Journal Section

Research Article

Authors

Suresh Shenvi ^*
0000-0003-4466-774X
India

Sonal B Joshi
0000-0002-9660-2705
India

Kavalipurapu Venkata Teja
0000-0000-0000-0000
India

Publication Date

September 30, 2026

Submission Date

June 12, 2026

Acceptance Date

August 31, 2026

Published in Issue

Year 2026 Volume: 29 Number: 3

DOI

https://doi.org/10.7126/cumudj.1968792

IZ

https://izlik.org/JA27RN42DE

Cite

RIS / Bibtex

APA

Shenvi, S., Joshi, S. B., & Venkata Teja, K. (2026). Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study. Cumhuriyet Dental Journal, 29(3), 512-517. https://doi.org/10.7126/cumudj.1968792

AMA

1.Shenvi S, Joshi SB, Venkata Teja K. Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study. Cumhuriyet Dent J. 2026;29(3):512-517. doi:10.7126/cumudj.1968792

Chicago

Shenvi, Suresh, Sonal B Joshi, and Kavalipurapu Venkata Teja. 2026. “Impact of N-Acetylcysteine and EDTA As Final Irrigation Solutions on Push-Out Bond Strength of Two Endodontic Sealers: An In Vitro Study”. Cumhuriyet Dental Journal 29 (3): 512-17. https://doi.org/10.7126/cumudj.1968792.

EndNote

Shenvi S, Joshi SB, Venkata Teja K (September 1, 2026) Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study. Cumhuriyet Dental Journal 29 3 512–517.

IEEE

[1]S. Shenvi, S. B. Joshi, and K. Venkata Teja, “Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study”, Cumhuriyet Dent J, vol. 29, no. 3, pp. 512–517, Sept. 2026, doi: 10.7126/cumudj.1968792.

ISNAD

Shenvi, Suresh - Joshi, Sonal B - Venkata Teja, Kavalipurapu. “Impact of N-Acetylcysteine and EDTA As Final Irrigation Solutions on Push-Out Bond Strength of Two Endodontic Sealers: An In Vitro Study”. Cumhuriyet Dental Journal 29/3 (September 1, 2026): 512-517. https://doi.org/10.7126/cumudj.1968792.

JAMA

1.Shenvi S, Joshi SB, Venkata Teja K. Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study. Cumhuriyet Dent J. 2026;29:512–517.

MLA

Shenvi, Suresh, et al. “Impact of N-Acetylcysteine and EDTA As Final Irrigation Solutions on Push-Out Bond Strength of Two Endodontic Sealers: An In Vitro Study”. Cumhuriyet Dental Journal, vol. 29, no. 3, Sept. 2026, pp. 512-7, doi:10.7126/cumudj.1968792.

Vancouver

1.Suresh Shenvi, Sonal B Joshi, Kavalipurapu Venkata Teja. Impact of N-Acetylcysteine and EDTA as Final Irrigation Solutions on Push-Out Bond Strength of two endodontic sealers: An In Vitro study. Cumhuriyet Dent J. 2026 Sep. 1;29(3):512-7. doi:10.7126/cumudj.1968792