Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry

Arigela Sai Vamsi; Aysha Jebin A; M.l. Prabhuji

doi:10.7126/cumudj.1676547

EN

Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry

Abstract

OBJECTIVES: In recent years there has been a growing interest in the use of platelet rich products for the treatment of many clinical conditions in dentistry. But there are possible chances of contamination of these products with silica. This study we aimed to define the structural characteristics and elemental analysis of platelet-rich product prepared in titanium tubes, Titanium-Platelet Rich Fibrin (T-PRF) and compare it with Leukocyte Platelet Rich Fibrin (L-PRF). METHODS: In this study blood is collected from the selected 10 patients through anti-cubital vein and used to detect the titanium and silica composition in T-PRF and L-PRF using Inductively Coupled Plasma – Optical Emission Spectrometry [ICP-OES]. The study also compared the structural difference between fibrin network in T-PRF and L-PRF using Phosphotungstic Acid Hematoxylin staining [PTAH] and hematoxylin and eosin staining method. RESULTS: On ICP-OES analysis there was a difference in the mean value of titanium ions and silica ions between the two groups. Almost 18.24 ppm of Titanium ions and 0.611ppm of silica ions were seen in the T-PRF group and 36.2 ppm of Silica ions and 3.56 ppm of titanium ions were seen in L-PRF group. On histological examination using haematoxylin and eosin and PTAH staining and focused under microscope there is significant structural difference in fibrin mesh formation and thickness was seen between T-PRF and L-PRF.This difference was statistically significant. CONCLUSION: This study is first of its kind analyzing the titanium and silica ions in L PRF and T-PRF using ICP -OES analysis, which will contribute to the improvement of L-PRF and its biocompatibility. This study emphasized that, T-PRF is having a strong fibrin network, with less potential contamination with silica. And it is safest regenerative material to use in clinical practice.

Keywords

Supporting Institution

Krishnadevaraya college of dental sciences and hospital Bangalore India

Ethical Statement

The study was approved by Krishnadevaraya College of Dental Sciences Ethical Committee, Bangalore, (KCDSHEC/IP/2024/6A) .

Thanks

The authors express their heartfelt gratitude to DR. RADHIKA M BAVLE (Professor & Head, Department of Oral & Maxillofacial Pathology, Krishnadevaraya College of Dental Sciences and Hospital) for helping in the structural analysis of PRF products using Phosphotungstic Acid Hematoxylin staining [PTAH] and hematoxylin and eosin staining method. We express our sincere thanks to DR. SEETAPALLI MALLIKARJUNA, (General Manager, Shiva Analyticals Lab) for conducting the ICP-OES Analysis for this study.

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Details

Primary Language

English

Subjects

Dental Materials and Equipment, Periodontics

Journal Section

Research Article

Authors

Arigela Sai Vamsi
0009-0000-7879-7839
India

Aysha Jebin A ^*
0000-0003-1036-242X
India

M.l. Prabhuji
0000-0002-0353-8119
India

Publication Date

July 1, 2026

Submission Date

April 16, 2025

Acceptance Date

April 13, 2026

Published in Issue

Year 2026 Volume: 29 Number: 2

DOI

https://doi.org/10.7126/cumudj.1676547

IZ

https://izlik.org/JA62DH54HD

Cite

RIS / Bibtex

APA

Sai Vamsi, A., Jebin A, A., & Prabhuji, M. (2026). Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry. Cumhuriyet Dental Journal, 29(2), 201-208. https://doi.org/10.7126/cumudj.1676547

AMA

1.Sai Vamsi A, Jebin A A, Prabhuji M. Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry. Cumhuriyet Dent J. 2026;29(2):201-208. doi:10.7126/cumudj.1676547

Chicago

Sai Vamsi, Arigela, Aysha Jebin A, and M.l. Prabhuji. 2026. “Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative Evaluation of Structural and Elemental Characteristics Using Inductively Coupled Plasma Optical Emission Spectrometry”. Cumhuriyet Dental Journal 29 (2): 201-8. https://doi.org/10.7126/cumudj.1676547.

EndNote

Sai Vamsi A, Jebin A A, Prabhuji M (July 1, 2026) Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry. Cumhuriyet Dental Journal 29 2 201–208.

IEEE

[1]A. Sai Vamsi, A. Jebin A, and M. Prabhuji, “Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry”, Cumhuriyet Dent J, vol. 29, no. 2, pp. 201–208, July 2026, doi: 10.7126/cumudj.1676547.

ISNAD

Sai Vamsi, Arigela - Jebin A, Aysha - Prabhuji, M.l. “Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative Evaluation of Structural and Elemental Characteristics Using Inductively Coupled Plasma Optical Emission Spectrometry”. Cumhuriyet Dental Journal 29/2 (July 1, 2026): 201-208. https://doi.org/10.7126/cumudj.1676547.

JAMA

1.Sai Vamsi A, Jebin A A, Prabhuji M. Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry. Cumhuriyet Dent J. 2026;29:201–208.

MLA

Sai Vamsi, Arigela, et al. “Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative Evaluation of Structural and Elemental Characteristics Using Inductively Coupled Plasma Optical Emission Spectrometry”. Cumhuriyet Dental Journal, vol. 29, no. 2, July 2026, pp. 201-8, doi:10.7126/cumudj.1676547.

Vancouver

1.Arigela Sai Vamsi, Aysha Jebin A, M.l. Prabhuji. Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry. Cumhuriyet Dent J. 2026 Jul. 1;29(2):201-8. doi:10.7126/cumudj.1676547