Research Article

Investigation of Cytotoxic Effects and Antimicrobial Activities of Light-cured and Self-cured Universal Adhesive Systems

Volume: 27 Number: 3 September 30, 2024
Cumhuriyet Dental Journal Cover
Cumhuriyet Dental Journal
PDF Download
EN

Investigation of Cytotoxic Effects and Antimicrobial Activities of Light-cured and Self-cured Universal Adhesive Systems

Abstract

Objective: This study aimed to compare the cytotoxicity and antimicrobial activity of a light-cured adhesive system and a self-cured adhesive system from the same company. Materials and Methods: A Tokuyama BOND force II (Light-cured) adhesive system (TF2B) and a Tokuyama Universal Bond (Self-cured) adhesive system (TUB) were selected for the study. The cytotoxicity evaluation of these two systems on cell cultures was performed using MTT assay and Agar Diffusion assay in L929 fibroblast cells. Disk diffusion method and liquid microdilution (MIC) method were used to evaluate their antimicrobial activity. The experiments were performed on 6 pathogenic bacteria and 1 yeast fungus. Results: According to MTT test results, both adhesive systems have no significant toxic effect on healthy cells (L929). However, when TUB and TF2B were compared with each other, it was found that TF2B had almost no toxic effect. In the agar diffusion test, when the two bonds were compared with each other, a weak color lightening was observed only around the first concentration of TUB. No visible melting was detected in other concentrations of TUB and TF2B. Both adhesive systems failed to reach MIC values effectively on the test microorganisms. Since the results were far above the MIC values of the reference antibiotics, it was determined that they did not have antimicrobial effects. Disk diffusion results similarly showed that both bonds did not form an inhibition zone on the test microorganisms. Conclusions: In dentistry, cytotoxic effects of universal adhesive systems on living cells can be observed. Self-cured and Light-cured adhesive systems did not show toxic effects on L929 cells. In addition, antimicrobial effects on test microorganisms were not detected. The cytotoxicity of the materials can be tested on different cells.

Keywords

References

  1. 1. Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J. Biomed. Mater. Res. 2008;86:523-529
  2. 2. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeek B, De Munck J. Bonding effectiveness of a new ‘multi-mode’adhesive to enamel and dentine. J. Dent. 2012;40:475-484
  3. 3. Perdigão J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multipurpose dentin adhesive. Am. J. Dent. 2012;25:153
  4. 4. Perdigão J, Muñoz MA, Sezinando A, Luque-Martinez IV, Staichak R, Reis A, Loguercio AD. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper. Dent. 2014;39:489-499
  5. 5. Caughman WF, Rueggeberg F. Shedding new light on composite polymerization. Oper. Dent. 2002;27:636-638
  6. 6. Mills RW, Uhl A, Blackwell GB, Jandt KD. High power light emitting diode (LED) arrays versus halogen light polymerization of oral biomaterials: Barcol hardness, compressive strength and radiometric properties. Biomaterials. 2002;23:2955-2963
  7. 7. Wataha JC. Principles of biocompatibility for dental practitioners. J. Prosthet. Dent. 2001;86:203-209
  8. 8. Edgerton M, Levine MJ. Biocompatibility: its future in prosthodontic research. The J. Prosthet. Dent. 1993;69:406-415

Details

Primary Language

English

Subjects

Restorative Dentistry , Dental Materials , Dentistry (Other)

Journal Section

Research Article

Authors

Publication Date

September 30, 2024

Submission Date

July 16, 2024

Acceptance Date

August 11, 2024

Published in Issue

Year 2024 Volume: 27 Number: 3

DOI

https://doi.org/10.7126/cumudj.1516900

IZ

https://izlik.org/JA93GH99HX

Cite

RIS / Bibtex
EndNote
Tunç T, Bal H, Hubbezoğlu İ (September 1, 2024) Investigation of Cytotoxic Effects and Antimicrobial Activities of Light-cured and Self-cured Universal Adhesive Systems. Cumhuriyet Dental Journal 27 3 201–208.

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


CDJ accepts articles in English. Submitting a paper to CDJ is free of charges. In addition, CDJ has not have article processing charges.

Frequency: Four times a year (March, June, September, and December)

IMPORTANT NOTICE

All users of Cumhuriyet Dental Journal should visit to their user's home page through the "https://dergipark.org.tr/tr/user" " or "https://dergipark.org.tr/en/user" links to update their incomplete information shown in blue or yellow warnings and update their e-mail addresses and information to the DergiPark system. Otherwise, the e-mails from the journal will not be seen or fall into the SPAM folder. Please fill in all missing part in the relevant field.

Please visit journal's AUTHOR GUIDELINE to see revised policy and submission rules to be held since 2020.