The Effect of Photodynamic Therapy Applied with Different Photosensitizers on Dentin Hardness in Comparison with Conventional Irrigation

Cihan Küden

doi:10.7126/cumudj.1199515

EN TR

The Effect of Photodynamic Therapy Applied with Different Photosensitizers on Dentin Hardness in Comparison with Conventional Irrigation

Abstract

Objective: The aim of this study was to examine the effect of photodynamic therapy (PDT) using toluidine blue O (TBO), curcumin (CUR) and methylene blue (MB) photosensitizers on root canal dentin microhardness by comparing it with sodium hypochlorite (NaOCl) + ethylenediamine tetraacetic acid (EDTA). Materials and methods: The root canals of 100 human premolar teeth were shaped by the R25 file (Reciproc; VDW, Munich, Germany). The working length of the teeth was determined by using a #10 K-file, keeping it 1mm shorter than the tooth apex. The R25 file was used to prepare the root canals. After every three pecking motions, irrigation was performed and a total of 10 mm of distilled water was used. The specimens were randomly distributed according to the disinfection method; NaOCl+EDTA, PDT with TBO, PDT with CUR, PDT with MB and distilled water (DS) (n=20). Grooves were prepared on the buccal and lingual surfaces of the prepared teeth, parallel to the long axis of the tooth, without touching the root canals. The roots were divided into two parts by means of a cement spatula placed in these grooves. Root canal dentin microhardness was evaluated by the Vickers test method. Three measurements were made by applying 300 g of force for 15 seconds and the average was calculated. It was recorded as the Vickers hardness value. The data were analyzed by using the one-way ANOVA and Dunnett’s post hoc tests (α=0.05). Results: All photosensitizer groups showed significantly higher microhardness value than the groups of DS and NaOCl + EDTA (p<0.05). There were no differences the groups of photosensitizers (p>0.05). Conclusions: PDT with CUR obtained the highest radicular dentin microhardness.

Keywords

Farklı Işığa Duyarlılaştırıcılar ile Uygulanan Foto Dinamik Terapinin Konvansiyonel İrrigasyon ile Karşılaştırılarak Dentin Sertliğine Etkisi

Öz

Amaç: Bu çalışmanın amacı, toluidin mavisi O (TBO), kurkumin (CUR) ve metilen mavisi (MB) ışığa duyarlılaştırıcılar kullanılarak yapılan fotodinamik tedavinin (PDT) kök kanal dentin mikrosertliği üzerindeki etkisini sodyum hipoklorit (NaOCl) + etilendiamin tetraasetik asit (EDTA) ile karşılaştırarak incelemektir. Gereç ve yöntem: 100 adet insan premolar dişinin kök kanalları R25 eğesi (Reciproc; VDW, Münih, Almanya) ile şekillendirildi. Dişlerin çalışma uzunluğu, diş apeksinden 1 mm daha kısa tutularak #10 K-file kullanılarak belirlendi. Kök kanallarını hazırlamak için R25 eğesi kullanıldı. Her üç gagalama hareketinden sonra irrigasyon yapıldı ve toplam 10 mm distile su kullanıldı. Numuneler dezenfeksiyon yöntemine göre rastgele dağıtıldı; NaOCl+EDTA, TBO ile PDT, CUR ile PDT, MB ve distile su ile PDT (DS) (n=20). Hazırlanan dişlerin bukkal ve lingual yüzeylerine, dişin uzun eksenine paralel olacak şekilde, kök kanallarına dokunmadan oluklar hazırlandı. Bu oluklara yerleştirilen siman spatülü ile kökler iki parçaya bölündü. Kök kanal dentin mikrosertliği Vickers test yöntemi ile değerlendirildi. 15 saniye boyunca 300 g kuvvet uygulanarak üç ölçüm yapıldı ve ortalaması hesaplandı. Vickers sertlik değeri olarak kaydedildi. Veriler, tek yönlü ANOVA ve Dunnett'in post hoc testleri (α=0.05) kullanılarak analiz edildi. Bulgular: Tüm ışığa duyarlılaştırıcı grupları, DS ve NaOCl + EDTA gruplarından önemli ölçüde daha yüksek mikrosertlik değeri gösterdi (p<0.05). Işığa duyarlılaştırıcı grupları arasında fark bulunmadı (p>0.05). Sonuçlar: CUR ile PDT, en yüksek radiküler dentin mikrosertliği gösterdi.

Anahtar Kelimeler

Thanks

I would like to thank Seda Nur Karakaş for her support.

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Cihan Küden ^*
0000-0002-2663-9828
Türkiye

Publication Date

December 29, 2022

Submission Date

November 7, 2022

Acceptance Date

December 16, 2022

Published in Issue

Year 2022 Volume: 25 Number: Suppl/2

DOI

https://doi.org/10.7126/cumudj.1199515

IZ

https://izlik.org/JA98FY43RU

Cite

RIS / Bibtex

EndNote

Küden C (December 1, 2022) The Effect of Photodynamic Therapy Applied with Different Photosensitizers on Dentin Hardness in Comparison with Conventional Irrigation. Cumhuriyet Dental Journal 25 Suppl/2 26–31.

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