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Seçili Bazı Kırmızı Meyve ve Sebzelerde Total Antioksidan-Oksidan Durumun ve Antimikrobiyal Aktivitenin İncelenmesi

Yıl 2022, Cilt: 2 Sayı: 1, 1 - 12, 28.01.2022

Öz

Amaç: Bu çalışma, seçili bazı kırmızı meyve/sebzelerin etanolik ekstraktlarındaki total antioksidan durumu (TAS); total oksidan durumu (TOS) ve oksidatif stres indeksini (OSI) belirlemeyi; ayrıca bu ekstraktların antimikrobiyal etkisini değerlendirmeyi amaçlamıştır.
Gereç ve Yöntem: Meyve/sebzelerin etanolik ekstraktı Soxhlet cihazı ile elde edildi. TAS, TOS ve OSI, Rel Assay kitleri kullanılarak belirlendi. Ekstraktların antimikrobiyal aktivitesi, agar difüzyon yöntemi kullanılarak iki Gram pozitif (Staphylococcus aureus, Bacillus subtilis), bir Gram negatif bakteri (Escherichia coli) ve bir mantar (Candida albicans) suşlarına karşı tarandı.
Bulgular: En yüksek TAS (8.407±.0.399 mmol/L), TOS (7.277.±0.244 μmol/L) ve OSI (0.157±.0.019) değerleri sırasıyla kırmızı lahana, kırmızı soğan ve kırmızı turpta belirlendi. En düşük TAS (3.668±.0.437), TOS (3.769±.0.568) ve OSI (0.058±0.002) değerleri sırasıyla kırmızı turp, kırmızı nar ve kırmızı havuçta elde edildi. Kırmızı pancar ve lahana antimikrobiyal aktivite göstermedi. Nar ekstraktı, Gram negatif bakteri ve mantarların büyümesini seçici olarak engelledi ve en duyarlı suş 21 mm inhibisyon zonu ile E.coli oldu. Havuç, soğan ve turp ekstraktları B.subtilis (12-13mm) ve E.coli (12-13mm) de benzer sonuçlar verdi. Soğan ve turp ekstractı antifungal aktivite göstermedi.
Sonuç: Kırmızı lahananın antioksidan potansiyelinin çok yüksek olduğu söylenebilir. Ayrıca nar ve kırmızı havucun düşük oksidatif stres indeksleri, bitkideki endojen antioksidanların endojen oksidanları önemli ölçüde inhibe ettiğini göstermiştir. Narın antibiyotikler kadar hatta bazılarından daha fazla antimikrobiyal olduğu görülmüştür.

Teşekkür

Çalışma Ahi Evran Üniversitesi Fen Edebiyat Fakültesi Mikrobiyoloji Araştırma Laboratuvarı’nda yapılmıştır. Mikrobiyolojik çalışmalarda desteği ve yardımlarından dolayı Dr Öğr Üyesi Esin Kiray’a teşekkür ederim.

Kaynakça

  • Referans1 Adly AA. Oxidative stress and disease: an updated review. Res J Immunol. 2010; 3(2): 129–45.
  • Referans2 Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, et al. Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Front Physiol. 2020; 11: 694.
  • Referans3 Kusano C, Ferrari B. Total antioxidant capacity: a biomarker in biomedical and nutritional studies. Journal of Cell and Molecular Biology. 2008; 7(1): 1–15.
  • Referans4 Gülçin, İ. Antioxidant activity of l-adrenaline: A structure–activity insight. Chem Biol Interact. 2009; 179 (2-3): 71–80.
  • Referans5 Soccio M, Laus MN, Alfarano M, Dalfino G, Panunzio MF, et al. Antioxidant/Oxidant Balance as a novel approach to evaluate the effect on serum of long-term intake of plant antioxidant-rich foods. Journal of Functional Foods. 2009; 40: 778–784.
  • Referans6 Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry. 2005; 29(4): 297–303.
  • Referans7 Salomoni R, Léo P, Montemor AF, Rinaldi BG, Rodrigues M. Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa. Nanotechnol Sci Appl. 2017; 10: 115–21.
  • Referans8 Fan W, Yung B, Huang P, Chen X. Nanotechnology for multimodal synergistic cancer therapy. Chem Rev. 2017; 117(22): 13566–638.
  • Referans9 Deshpande RR, Kale AA, Ruikaretal AD. Antimicrobial activity of different extracts of Juglans regia L. against oral Microflora. Int J Pharm Pharm Sci. 2011; 3(2): 200–201.
  • Referans10 Meziane S, Magand J, Mathé M, Raimond S, Yvon JL, Kaci MM. Bioavailability of natural and synthetic vitamins: A significant difference on Oxidative Stress Status (OSS). Integr Food Nutr Metab. 2021; 8(IFNM): 2–10.
  • Referans11 Faydaoğlu E, Sürücüoğlu M. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan Üniv Fen Bilim Enst Derg. 2013; 6(2): 233–265.
  • Referans12 Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4) :277–85.
  • Referans13 Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38: 1103–1111.
  • Referans14 Holder IA, Boyce ST. Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns. 1994; 20(5): 426–9.
  • Referans15 Hussain T, Tan B, Yin Y, Blachier F, Tossou MCB, Rahu N. Oxidative stress and inflammation: What polyphenols can do for us? Oxid Med Cell Longev. 2016; 2016: 7432797.
  • Referans16 Lourenço SC, Moldão-Martins M, Alves VD. Antioxidants of natural plant origins: From sources to food industry applications. Molecules. 2019; 24(22): 4132.
  • Referans17 Karaaslan M, Vardin H, Varlıklıöz S, Yılmaz FM. Antiproliferative and antioxidant activities of Turkish pomegranate (Punica granatum L. ) accessions. Int J Food Sci Technol. 2014; 49: 82–90.
  • Referans18 Wu Q, Song R, Zhao L, Yun Z. Advances in cellular evaluation and standard of antioxidant activity. E3S Web Conf. 2019; 131: 01008.
  • Referans19 Zhang H, Yin M, Huang L, Wang J, Gong L, Liu J, et al. Evaluation of the cellular and animal models for the study of antioxidant activity: A review. J Food Sci. 2017; 82(2): 278–88.
  • Referans20 Mohammed FS, Şabik AE, Sevindik E, Pehlivan M, Sevindik M. Determination of antioxidant and oxidant potentials of Thymbra spicata collected from Duhok-Iraq. Turkish Journal of Agriculture-Food Science and Technology. 2020; 8(5): 1171–1173.
  • Referans21 Gürgen, A, Sevindik, M, Yıldız, S, Akgül, H. Determination of antioxidant and oxidant potentials of Pleurotus citrinopileatus mushroom cultivated on various substrates. KSÜ Tarım ve Doğa Dergisi. 2020; 23(3): 586–591.
  • Referans22 Pehlivan M, Mohammed FS, Sevi̇ndi̇k M, Akgul H. Antioxidant and oxidant potential of Rosa canina. Eurasian J For Sci. 2018; 6(4): 22–25. Referans23 Dikilitas M, Guldur ME, Deryaoglu A, Ozcan EREL. Antioxidant and oxidant levels of pepper (Capsicum annuum cv. ’Charlee’) infected with pepper mild mottle virus. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2011; 39(2): 58–63.
  • Referans24 Mohammed FS. Phenolic contents, antioxidant and antimicrobial activıties of Allium stamineum collected from Duhok. Iraq. Fresenius Environmental Bulletin. 2020; 29(09): 7526–7531.
  • Referans25 Yılmaz İ. Antioksidan içeren bazı gıdalar ve oksidatif stres. İnönü Üniversitesi Tıp Fakültesi Dergisi. 2010; 17 (2): 143–153.
  • Referans26 Şengün İY, Yücel E. Antimicrobial properties of wild fruits. Biological Diversity and Conservation. 2015; 8(1): 69–77.
  • Referans27 Česonienė, L, Daubaras, R, Kraujalytė, V, Venskutonis, PR, Šarkinas, A. Antimicrobial activity of Viburnum opulus fruit juices and extracts. J Verbraucherschutz Leb. 2014; 9(2): 129–132.

Investigation of Total Antioxidant-Oxidant Capacity and Antimicrobial Activity in Some Selected Red Fruit and Vegetables

Yıl 2022, Cilt: 2 Sayı: 1, 1 - 12, 28.01.2022

Öz

Purpose: This study aimed to determine the total antioxidant capacity (TAC), total oxidant capacity (TOC) and the oxidative stress index (OSI) of ethanolic extracts of some selected red fruit/vegetables; it also aimed to evaluate the antimicrobial effect of this extracts.
Materials and Methods: The fruit/vegetables were extracted with ethanol (EtOH) using a Soxhlet device. TAC, TOC and OSI were determined using Rel Assay kits. The antimicrobial activity of the extracts was screened against two Gram-positive (Staphylococcus aureus, Bacillus subtilis), one Gram-negative bacteria (Escherichia coli) and one fungi (Candida albicans) strains using the agar diffusion method.
Results: The highest TAC (8.407±0.399 mmol/L), TOC (7.277±0.244 µmol/L) and OSI (0.157±0.019) values were determined in red cabbage, red onion, and red radish, respectively. The lowest TAC (3.668±0.437), TOC (3.769±0.568) and OSI (0.058±0.002) values were obtained in red radish, red pomegranate, and red carrot, respectively. Red beets and cabbage showed no antimicrobial activity. Pomegranate extract selectively inhibited the growth of Gram-negative bacteria and fungi, being E. coli the most susceptible one with 21 mm of inhibition zone. Carrot, onion and radish extracts gave similar results on B. subtilis (12-13mm) and E. coli (12-13mm). Onions and radishes extracts do not show anti-fungal activity.
Conclusion: It can be said that the antioxidant potential of red cabbage is very high. In addition, low oxidative stress index of pomegranate and red carrot have shown that endogenous antioxidants in the plant significantly inhibit endogenous oxidants. Pomegranate has been found to be as antimicrobial as antibiotics, even more than some.

Kaynakça

  • Referans1 Adly AA. Oxidative stress and disease: an updated review. Res J Immunol. 2010; 3(2): 129–45.
  • Referans2 Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, et al. Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Front Physiol. 2020; 11: 694.
  • Referans3 Kusano C, Ferrari B. Total antioxidant capacity: a biomarker in biomedical and nutritional studies. Journal of Cell and Molecular Biology. 2008; 7(1): 1–15.
  • Referans4 Gülçin, İ. Antioxidant activity of l-adrenaline: A structure–activity insight. Chem Biol Interact. 2009; 179 (2-3): 71–80.
  • Referans5 Soccio M, Laus MN, Alfarano M, Dalfino G, Panunzio MF, et al. Antioxidant/Oxidant Balance as a novel approach to evaluate the effect on serum of long-term intake of plant antioxidant-rich foods. Journal of Functional Foods. 2009; 40: 778–784.
  • Referans6 Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry. 2005; 29(4): 297–303.
  • Referans7 Salomoni R, Léo P, Montemor AF, Rinaldi BG, Rodrigues M. Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa. Nanotechnol Sci Appl. 2017; 10: 115–21.
  • Referans8 Fan W, Yung B, Huang P, Chen X. Nanotechnology for multimodal synergistic cancer therapy. Chem Rev. 2017; 117(22): 13566–638.
  • Referans9 Deshpande RR, Kale AA, Ruikaretal AD. Antimicrobial activity of different extracts of Juglans regia L. against oral Microflora. Int J Pharm Pharm Sci. 2011; 3(2): 200–201.
  • Referans10 Meziane S, Magand J, Mathé M, Raimond S, Yvon JL, Kaci MM. Bioavailability of natural and synthetic vitamins: A significant difference on Oxidative Stress Status (OSS). Integr Food Nutr Metab. 2021; 8(IFNM): 2–10.
  • Referans11 Faydaoğlu E, Sürücüoğlu M. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan Üniv Fen Bilim Enst Derg. 2013; 6(2): 233–265.
  • Referans12 Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4) :277–85.
  • Referans13 Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38: 1103–1111.
  • Referans14 Holder IA, Boyce ST. Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns. 1994; 20(5): 426–9.
  • Referans15 Hussain T, Tan B, Yin Y, Blachier F, Tossou MCB, Rahu N. Oxidative stress and inflammation: What polyphenols can do for us? Oxid Med Cell Longev. 2016; 2016: 7432797.
  • Referans16 Lourenço SC, Moldão-Martins M, Alves VD. Antioxidants of natural plant origins: From sources to food industry applications. Molecules. 2019; 24(22): 4132.
  • Referans17 Karaaslan M, Vardin H, Varlıklıöz S, Yılmaz FM. Antiproliferative and antioxidant activities of Turkish pomegranate (Punica granatum L. ) accessions. Int J Food Sci Technol. 2014; 49: 82–90.
  • Referans18 Wu Q, Song R, Zhao L, Yun Z. Advances in cellular evaluation and standard of antioxidant activity. E3S Web Conf. 2019; 131: 01008.
  • Referans19 Zhang H, Yin M, Huang L, Wang J, Gong L, Liu J, et al. Evaluation of the cellular and animal models for the study of antioxidant activity: A review. J Food Sci. 2017; 82(2): 278–88.
  • Referans20 Mohammed FS, Şabik AE, Sevindik E, Pehlivan M, Sevindik M. Determination of antioxidant and oxidant potentials of Thymbra spicata collected from Duhok-Iraq. Turkish Journal of Agriculture-Food Science and Technology. 2020; 8(5): 1171–1173.
  • Referans21 Gürgen, A, Sevindik, M, Yıldız, S, Akgül, H. Determination of antioxidant and oxidant potentials of Pleurotus citrinopileatus mushroom cultivated on various substrates. KSÜ Tarım ve Doğa Dergisi. 2020; 23(3): 586–591.
  • Referans22 Pehlivan M, Mohammed FS, Sevi̇ndi̇k M, Akgul H. Antioxidant and oxidant potential of Rosa canina. Eurasian J For Sci. 2018; 6(4): 22–25. Referans23 Dikilitas M, Guldur ME, Deryaoglu A, Ozcan EREL. Antioxidant and oxidant levels of pepper (Capsicum annuum cv. ’Charlee’) infected with pepper mild mottle virus. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2011; 39(2): 58–63.
  • Referans24 Mohammed FS. Phenolic contents, antioxidant and antimicrobial activıties of Allium stamineum collected from Duhok. Iraq. Fresenius Environmental Bulletin. 2020; 29(09): 7526–7531.
  • Referans25 Yılmaz İ. Antioksidan içeren bazı gıdalar ve oksidatif stres. İnönü Üniversitesi Tıp Fakültesi Dergisi. 2010; 17 (2): 143–153.
  • Referans26 Şengün İY, Yücel E. Antimicrobial properties of wild fruits. Biological Diversity and Conservation. 2015; 8(1): 69–77.
  • Referans27 Česonienė, L, Daubaras, R, Kraujalytė, V, Venskutonis, PR, Šarkinas, A. Antimicrobial activity of Viburnum opulus fruit juices and extracts. J Verbraucherschutz Leb. 2014; 9(2): 129–132.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Elif Azize Özşahin Delibaş

Yayımlanma Tarihi 28 Ocak 2022
Gönderilme Tarihi 23 Kasım 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 2 Sayı: 1

Kaynak Göster

Vancouver Özşahin Delibaş EA. Seçili Bazı Kırmızı Meyve ve Sebzelerde Total Antioksidan-Oksidan Durumun ve Antimikrobiyal Aktivitenin İncelenmesi. TOGÜ Sağlık Bilimleri Dergisi. 2022;2(1):1-12.