Dentin Hassasiyet Gidericilerin Sığır Pulpasından Türetilen Hücre Canlılığı Üzerindeki Sitotoksik Etkisi
Öz
Amaç: Mevcut çalışmada sığır pulpasından elde edilmiş hücreler üzerinde dentin hassasiyet giderici ajanların sitotoksisitesi değerlendirdi.
Gereç ve Yöntemler: Sitotoksisite testi için, SV40 (Simian virüsü 40) büyük T antijeni ile transfekte edilmiş sığır pulpasından elde edilmiş hücreler Shield Force Plus, Gluma ve Teethmate Desensitizer’in farklı dilüsyonlarına (orjinal, % ½ ve % ½ ) 24 saat boyunca maruz bırakıldı. Kontrol grubu olarak tam hücre kültür ortamı kullanıldı. Sığır pulpasından elde edilmiş hücrelerin canlılığı MTT testi ile belirlendi. Veriler tek yönlü varyans analizi (ANOVA) ve Tukey HSD testleri kullanılarak analiz edildi.
Bulgular: Shield Force Plus ve Teethmate Desensitizer grupları, kontrol ile karşılaştırıldığında sığır pulpasından elde edilmiş hücreler sağkalımını değiştirmedi (p>0.05). Gluma, tüm konsantrasyonlarda sığır pulpasından elde edilmiş hücreler üzerinde sitotoksik etki gösterdi (p<0.05).
Sonuçlar: Shield Force Plus ve Teethmate Desensitizer, sığır pulpasından elde edilmiş hücreler üzerine Gluma'dan daha biyouyumludur.
Anahtar Kelimeler
Kaynakça
- 1. West N, Lussi A, Seong J, Hellwig E. Dentin hypersensitivity: pain mechanisms and aetiology of exposed cervical dentin. Clinical oral investigations. 2013;17(1):9-19.
- 2. Liu XX, Tenenbaum HC, Wilder RS, Quock R, Hewlett ER, Ren Y-F. Pathogenesis, diagnosis and management of dentin hypersensitivity: an evidence-based overview for dental practitioners. BMC oral health. 2020;20(1):1-10.
- 3. Jiang R, Xu Y, Wang F, Lin H. Effectiveness and cytotoxicity of two desensitizing agents: a dentin permeability measurement and dentin barrier testing in vitro study. BMC Oral Health. 2022;22(1):1-11.
- 4. Thonemann B, Schmalz G. Immortalization of bovine dental papilla cells with simian virus 40 large t antigen. Archives of oral biology. 2000;45(10):857-69.
- 5. Arrais CAG, Chan DCN, Giannini M. Effects of desensitizing agents on dentinal tubule occlusion. Journal of Applied Oral Science. 2004;12(2):144-8.
- 6. Kaga M, Noda M, Ferracane J, Nakamura W, Oguchi H, Sano H. The in vitro cytotoxicity of eluates from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells. Dental Materials. 2001;17(4):333-9.
- 7. Noda M, Wataha JC, Lockwood PE, Volkmann KR, Kaga M, Sano H. Sublethal, 2-week exposures of dental material components alter TNF-α secretion of THP-1 monocytes. Dental Materials. 2003;19(2):101-5.
- 8. Eyüboğlu G, Yeşilyurt C, Ertürk M. Evaluation of cytotoxicity of dentin desensitizing products. Operative Dentistry. 2015;40(5):503-14.
- 9. Orchardson R, Gillam DG. Managing dentin hypersensitivity. The Journal of the American Dental Association. 2006;137(7):990-8.
- 10. Watanabe T, Sano M, Itoh K, Wakumoto S. The effects of primers on the sensitivity of dentin. Dental Materials. 1991;7(3):148-50.
- 11. Huang F, Chang Y. Cytotoxicity of dentine-bonding agents on human pulp cells in vitro. International Endodontic Journal. 2002;35(11):905-9.
- 12. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. Journal of dental research. 1995;74(9):1602-6.
- 13. Hirayama S, Takagi S, Markovic M, Chow LC. Properties of calcium phosphate cements with different tetracalcium phosphate and dicalcium phosphate anhydrous molar ratios. Journal of research of the National Institute of Standards and Technology. 2008;113(6):311.
- 14. Ishikawa K, Takagi S, Chow LC, Suzuki K. Reaction of calcium phosphate cements with different amounts of tetracalcium phosphate and dicalcium phosphate anhydrous. Journal of Biomedical Materials Research. 1999;46(4):504-10.
Öz
Aim: Present study evaluated the cytotoxicity of dentin desentisizing agents on bovine pulp-derived cells (bDPCs).
Materials and Methods: Transfected bDPCs cells were exposed to original, ½ and ¼ dilutions of Shield Force Plus, Gluma and Teethmate Desensitizer for 24 h. Culture medium was used as a control group. The bDPCs viability was tested by MTT after 24 hours of exposure. Data were analyzed using the one-way analysis of variance (ANOVA) and the Tukey HSD tests.
Results: Neither of the Shield Force Plus and Teethmate Desensitizers change the survival rate of bovine pulp-derived cells when compared to the control (p>0.05). Gluma Desensitizer have cytotoxic effects on bovine pulp-derived cells at all dilutions (p<0.05).
Conclusion: Shield Force Plus and Teethmate Desensitizer were more biocompatible than Gluma on bDPCs.
Anahtar Kelimeler
Kaynakça
- 1. West N, Lussi A, Seong J, Hellwig E. Dentin hypersensitivity: pain mechanisms and aetiology of exposed cervical dentin. Clinical oral investigations. 2013;17(1):9-19.
- 2. Liu XX, Tenenbaum HC, Wilder RS, Quock R, Hewlett ER, Ren Y-F. Pathogenesis, diagnosis and management of dentin hypersensitivity: an evidence-based overview for dental practitioners. BMC oral health. 2020;20(1):1-10.
- 3. Jiang R, Xu Y, Wang F, Lin H. Effectiveness and cytotoxicity of two desensitizing agents: a dentin permeability measurement and dentin barrier testing in vitro study. BMC Oral Health. 2022;22(1):1-11.
- 4. Thonemann B, Schmalz G. Immortalization of bovine dental papilla cells with simian virus 40 large t antigen. Archives of oral biology. 2000;45(10):857-69.
- 5. Arrais CAG, Chan DCN, Giannini M. Effects of desensitizing agents on dentinal tubule occlusion. Journal of Applied Oral Science. 2004;12(2):144-8.
- 6. Kaga M, Noda M, Ferracane J, Nakamura W, Oguchi H, Sano H. The in vitro cytotoxicity of eluates from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells. Dental Materials. 2001;17(4):333-9.
- 7. Noda M, Wataha JC, Lockwood PE, Volkmann KR, Kaga M, Sano H. Sublethal, 2-week exposures of dental material components alter TNF-α secretion of THP-1 monocytes. Dental Materials. 2003;19(2):101-5.
- 8. Eyüboğlu G, Yeşilyurt C, Ertürk M. Evaluation of cytotoxicity of dentin desensitizing products. Operative Dentistry. 2015;40(5):503-14.
- 9. Orchardson R, Gillam DG. Managing dentin hypersensitivity. The Journal of the American Dental Association. 2006;137(7):990-8.
- 10. Watanabe T, Sano M, Itoh K, Wakumoto S. The effects of primers on the sensitivity of dentin. Dental Materials. 1991;7(3):148-50.
- 11. Huang F, Chang Y. Cytotoxicity of dentine-bonding agents on human pulp cells in vitro. International Endodontic Journal. 2002;35(11):905-9.
- 12. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. Journal of dental research. 1995;74(9):1602-6.
- 13. Hirayama S, Takagi S, Markovic M, Chow LC. Properties of calcium phosphate cements with different tetracalcium phosphate and dicalcium phosphate anhydrous molar ratios. Journal of research of the National Institute of Standards and Technology. 2008;113(6):311.
- 14. Ishikawa K, Takagi S, Chow LC, Suzuki K. Reaction of calcium phosphate cements with different amounts of tetracalcium phosphate and dicalcium phosphate anhydrous. Journal of Biomedical Materials Research. 1999;46(4):504-10.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Diş Hekimliği |
| Bölüm | Araştırma |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Haziran 2023 |
| Gönderilme Tarihi | 11 Ocak 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 10 Sayı: 4 |
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