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Deneysel Sıçan Sepsis Modelinde Bromelainin Oksidatif Stres ve Akciğer Doku Histopatolojisi Üzerine Etkileri

Yıl 2023, Cilt: 16 Sayı: 3, 252 - 259, 30.09.2023
https://doi.org/10.30607/kvj.1302701

Öz

Sepsis, inflamatuvar bir sendrom olup başta akciğeri hedef almaktadır. Bromelainin (BRO) anti-inflamatuvar özelliği ile bilinen enzim kompleksidir. Bu çalışmada Sprague dawley sıçanlarında çekal ligasyon ve perforasyon (CLP) ile indüklenen sepsise bağlı akciğer hasarında BRO koruyucu etkisi biyokimyasal ve histopatolojik yöntemlerle araştırıldı. Kırk adet Sprague dawley sıçan, kontrol, CLP, BRO, CLP+BRO 50 ve CLP+BRO 100 olmak üzere rastgele beş gruba ayrıldı. Çalışmada sıçanlara 50 ve 100 mg/kg BRO oral olarak (7 gün boyunca toplam 7 uygulama) verildikten sonra laparotomi ve CLP uygulanmıştır. Veriler, BRO'nun antioksidan özelliklerinden dolayı CLP ile indüklenen miyeloperoksidaz (MPO) ve lipid peroksidasyonu (MDA) azalttığını (p <0,001), glutatyon (GSH) seviyelerini, süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) aktivitelerini arttırdığını göstermiştir (p <0,001). Ayrıca CLP kaynaklı akciğer histolojik değişiklikler BRO takviyesi ile azalmıştır. Sonuçlar BRO tedavisin CLP kaynaklı akciğer toksisitesi üzerinde antioksidan, anti-inflamatuvar etkiye sahip olduğunu ve düzenleyici etkisi sayesinde akciğer doku mimarisinin korunmasına yardımcı olduğunu göstermiştir.

Kaynakça

  • Agarwal, S., Chaudhary, B., & Bist, R. (2016). Bacoside A and bromelain relieve dichlorvos induced changes in oxidative responses in mice serum. Chemico-biological interactions, 254, 173–178. https://doi.org/10.1016/j.cbi.2016.05.017
  • Akaras, N., Bal, T., Atilay, H., Selli, J., & Halici, M. B. (2017). Protective effects of agomelatine on testicular damage caused by bortezomib. Biotechnic&histochemistry:official publication of the Biological Stain Commission, 92(8), 552–559. https://doi.org/10.1080/10520295.2017.1350748
  • Akaras, N., Gur, C., Kucukler, S., & Kandemir, F. M. (2023). Zingerone reduces sodium arsenite-induced nephrotoxicity by regulating oxidative stress, inflammation, apoptosis and histopathological changes. Chemico-biological interactions, 374, 110410. https://doi.org/10.1016/j.cbi.2023.110410
  • Anter, A., Ahmed, A. F., Hammad, A. S. A., Almalki, W. H., Abdel Hafez, S. M. N., Kasem, A. W., El-Moselhy, M. A., Alrabia, M. W., Ibrahim, A. R. N., & El-
  • Daly, M. (2022). The Severity of Acute Kidney and Lung Injuries Induced by Cecal Ligation and Puncture Is Attenuated by Menthol: Role of Proliferating Cell Nuclear Antigen and Apoptotic Markers. Frontiers in medicine, 9, 904286. https://doi.org/10.3389/fmed.2022.904286
  • Baradaran Rahimi, V., Rakhshandeh, H., Raucci, F., Buono, B., Shirazinia, R., Samzadeh Kermani, A., Maione, F., Mascolo, N., & Askari, V. R. (2019). Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury. Molecules (Basel, Switzerland), 24(1), 139. https://doi.org/10.3390/molecules24010139
  • Bradley, P. P., Priebat, D. A., Christensen, R. D., & Rothstein, G. (1982). Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. The Journal of investigative dermatology, 78(3), 206–209. https://doi.org/10.1111/1523-1747.ep12506462
  • Chen, G., Hou, Y., Li, X., Pan, R., & Zhao, D. (2021). Sepsis-induced acute lung injury in young rats is relieved by calycosin through inactivating the HMGB1/MyD88/NF-κB pathway and NLRP3 inflammasome. International immunopharmacology, 96, 107623. https://doi.org/10.1016/j.intimp.2021.107623
  • Chen, X., Wang, Y., Xie, X., Chen, H., Zhu, Q., Ge, Z., Wei, H., Deng, J., Xia, Z., & Lian, Q. (2018). Heme Oxygenase-1 Reduces Sepsis-Induced Endoplasmic Reticulum Stress and Acute Lung Injury. Mediators of inflammation, 2018, 9413876. https://doi.org/10.1155/2018/9413876
  • Cinar, I., Sirin, B., Aydin, P., Toktay, E., Cadirci, E., Halici, I., & Halici, Z. (2019). Ameliorative effect of gossypin against acute lung injury in experimental sepsis model of rats. Life sciences, 221, 327–334. https://doi.org/10.1016/j.lfs.2019.02.039
  • El-Demerdash, F. M., Baghdadi, H. H., Ghanem, N. F., & Mhanna, A. B. A. (2020). Nephroprotective role of bromelain against oxidative injury induced by aluminium in rats. Environmental toxicology and pharmacology, 80, 103509. https://doi.org/10.1016/j.etap.2020.103509
  • Eraslan, E., Tanyeli, A., Akdemir, F. N. E., & Güler, M. C. (2020). Reno-Protective Effects of 6-Shogaol on Kidney Tissue in Cecal Ligation and Puncture-Induced Polymicrobial Sepsis Rat Model. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(3), 244-250.
  • Ferah Okkay, I., Okkay, U., Bayram, C., Cicek, B., Sezen, S., Aydin, I. C., Mendil, A. S., & Hacimuftuoglu, A. (2023). Bromelain protects against cisplatin-induced ocular toxicity through mitigating oxidative stress and inflammation. Drug and chemical toxicology, 46(1), 69–76. https://doi.org/10.1080/01480545.2021.2011308
  • Gerin, F., Sener, U., Erman, H., Yilmaz, A., Aydin, B., Armutcu, F., & Gurel, A. (2016). The Effects of Quercetin on Acute Lung Injury and Biomarkers of Inflammation and Oxidative Stress in the Rat Model of Sepsis. Inflammation, 39(2), 700–705. https://doi.org/10.1007/s10753-015-0296-9
  • Giustina, A. D., Danielski, L. G., Novochadlo, M. M., Goldim, M. P. S., Joaquim, L., Metzker, K. L. L., Carli, R. J., Denicol, T., Cidreira, T., Vieira, T., & Petronilho, F. (2019). Vitamin B6 reduces oxidative stress in lungs and liver in experimental sepsis. Anais da Academia Brasileira de Ciencias, 91(4), e20190434. https://doi.org/10.1590/0001-3765201920190434
  • Gur, C., Kandemir, F. M., Caglayan, C., & Satıcı, E. (2022). Chemopreventive effects of hesperidin against paclitaxel-induced hepatotoxicity and nephrotoxicity via amendment of Nrf2/HO-1 and caspase-3/Bax/Bcl-2 signaling pathways. Chemico-biological interactions, 365, 110073. https://doi.org/10.1016/j.cbi.2022.110073
  • Cihan, G. Ü. R., Kandemir, Ö., & Kandemir, F. M. (2022). Ratlarda diklofenak ile indüklenen kardiyotoksisite üzerine krisinin etkilerinin oksidatif stres, endoplazmik retikulum stresi ve apoptoz belirteçleri ile değerlendirilmesi. Kocatepe Veterinary Journal, 15(2), 151-160.
  • Gur, C., Akarsu, S. A., Akaras, N., Tuncer, S. C., & Kandemir, F. M. (2023). Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite-induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environmental toxicology, 38(6), 1265–1276. https://doi.org/10.1002/tox.23762
  • Hu, Q., Wang, Q., Han, C., & Yang, Y. (2020). Sufentanil attenuates inflammation and oxidative stress in sepsis-induced acute lung injury by downregulating KNG1 expression. Molecular medicine reports, 22(5), 4298–4306. https://doi.org/10.3892/mmr.2020.11526
  • Kandemir, F. M., Caglayan, C., Aksu, E. H., Yildirim, S., Kucukler, S., Gur, C., & Eser, G. (2020). Protective effect of rutin on mercuric chloride-induced reproductive damage in male rats. Andrologia, 52(3), e13524. https://doi.org/10.1111/and.13524
  • Kandemir, F. M., Ileriturk, M., & Gur, C. (2022). Rutin protects rat liver and kidney from sodium valproate-induce damage by attenuating oxidative stress, ER stress, inflammation, apoptosis and autophagy. Molecular biology reports, 49(7), 6063–6074. https://doi.org/10.1007/s11033-022-07395-0
  • Khazaeel, K., Rad, O. R., Jamshidian, J., Tabandeh, M. R., Mohammadi, G., & Atashfaraz, A. (2022). Effect of bromelain on sperm quality, testicular oxidative stress and expression of oestrogen receptors in bisphenol-A treated male mice. Andrologia, 54(11), e14584. https://doi.org/10.1111/and.14584
  • Lawrence, R. A., & Burk, R. F. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and biophysical research communications, 71(4), 952–958. https://doi.org/10.1016/0006-291x(76)90747-6
  • Li, L., Wang, H., Zhao, S., Zhao, Y., Chen, Y., Zhang, J., Wang, C., Sun, N., & Fan, H. (2022). Paeoniflorin ameliorates lipopolysaccharide-induced acute liver injury by inhibiting oxidative stress and inflammation via SIRT1/FOXO1a/SOD2 signaling in rats. Phytotherapy research: PTR, 36(6), 2558–2571. https://doi.org/10.1002/ptr.7471
  • Li, R., Ren, T., & Zeng, J. (2019). Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3K/Akt/GSK-3β/mTOR Pathway in Rats. BioMed research international, 2019, 5240898. https://doi.org/10.1155/2019/5240898
  • Liu, Y., Mu, S., Li, X., Liang, Y., Wang, L., & Ma, X. (2019). Unfractionated Heparin Alleviates Sepsis-Induced Acute Lung Injury by Protecting Tight Junctions. The Journal of surgical research, 238, 175–185. https://doi.org/10.1016/j.jss.2019.01.020
  • Liu, Y., Zhang, Y., Feng, Q., Liu, Q., Xie, J., Li, H., Yang, F., Liu, X., Gao, W., Bai, X., Li, Z., & Wang, Y. (2021). GPA Peptide Attenuates Sepsis-Induced Acute Lung Injury in Mice via Inhibiting Oxidative Stress and Pyroptosis of Alveolar Macrophage. Oxidative medicine and cellular longevity, 2021, 5589472. https://doi.org/10.1155/2021/5589472
  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. The Journal of biological chemistry, 193(1), 265–275.
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Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model

Yıl 2023, Cilt: 16 Sayı: 3, 252 - 259, 30.09.2023
https://doi.org/10.30607/kvj.1302701

Öz

Sepsis is an inflammatory syndrome that targets the lung tissue. Bromelain (BRO) is an enzyme complex known for its anti-inflammatory properties. In this study, the protective effect of BRO on lung injury due to sepsis induced by cecal ligation and puncture (CLP) in Sprague-Dawley rats was investigated by biochemical and histopathological methods. Forty Sprague dawley rats were randomly divided into five groups as control, CLP, BRO, CLP+BRO 50 and CLP+BRO 100. In the study, after oral administration of 50 and 100 mg/kg BRO (total of 7 applications for 7 days) to rats, laparotomy and CLP were performed. The data showed that due to its antioxidant properties, BRO decreased CLP-induced myeloperoxidase (MPO) and lipid peroxidation (MDA) (p <0,001), increased glutathione (GSH) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities (p <0,001). Additionally, CLP-induced lung histological changes were reduced with BRO supplementation. The results showed that BRO treatment has an antioxidant, anti-inflammatory effect on CLP-induced lung toxicity and helps preserve lung tissue architecture owing to its regulatory effect.

Kaynakça

  • Agarwal, S., Chaudhary, B., & Bist, R. (2016). Bacoside A and bromelain relieve dichlorvos induced changes in oxidative responses in mice serum. Chemico-biological interactions, 254, 173–178. https://doi.org/10.1016/j.cbi.2016.05.017
  • Akaras, N., Bal, T., Atilay, H., Selli, J., & Halici, M. B. (2017). Protective effects of agomelatine on testicular damage caused by bortezomib. Biotechnic&histochemistry:official publication of the Biological Stain Commission, 92(8), 552–559. https://doi.org/10.1080/10520295.2017.1350748
  • Akaras, N., Gur, C., Kucukler, S., & Kandemir, F. M. (2023). Zingerone reduces sodium arsenite-induced nephrotoxicity by regulating oxidative stress, inflammation, apoptosis and histopathological changes. Chemico-biological interactions, 374, 110410. https://doi.org/10.1016/j.cbi.2023.110410
  • Anter, A., Ahmed, A. F., Hammad, A. S. A., Almalki, W. H., Abdel Hafez, S. M. N., Kasem, A. W., El-Moselhy, M. A., Alrabia, M. W., Ibrahim, A. R. N., & El-
  • Daly, M. (2022). The Severity of Acute Kidney and Lung Injuries Induced by Cecal Ligation and Puncture Is Attenuated by Menthol: Role of Proliferating Cell Nuclear Antigen and Apoptotic Markers. Frontiers in medicine, 9, 904286. https://doi.org/10.3389/fmed.2022.904286
  • Baradaran Rahimi, V., Rakhshandeh, H., Raucci, F., Buono, B., Shirazinia, R., Samzadeh Kermani, A., Maione, F., Mascolo, N., & Askari, V. R. (2019). Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury. Molecules (Basel, Switzerland), 24(1), 139. https://doi.org/10.3390/molecules24010139
  • Bradley, P. P., Priebat, D. A., Christensen, R. D., & Rothstein, G. (1982). Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. The Journal of investigative dermatology, 78(3), 206–209. https://doi.org/10.1111/1523-1747.ep12506462
  • Chen, G., Hou, Y., Li, X., Pan, R., & Zhao, D. (2021). Sepsis-induced acute lung injury in young rats is relieved by calycosin through inactivating the HMGB1/MyD88/NF-κB pathway and NLRP3 inflammasome. International immunopharmacology, 96, 107623. https://doi.org/10.1016/j.intimp.2021.107623
  • Chen, X., Wang, Y., Xie, X., Chen, H., Zhu, Q., Ge, Z., Wei, H., Deng, J., Xia, Z., & Lian, Q. (2018). Heme Oxygenase-1 Reduces Sepsis-Induced Endoplasmic Reticulum Stress and Acute Lung Injury. Mediators of inflammation, 2018, 9413876. https://doi.org/10.1155/2018/9413876
  • Cinar, I., Sirin, B., Aydin, P., Toktay, E., Cadirci, E., Halici, I., & Halici, Z. (2019). Ameliorative effect of gossypin against acute lung injury in experimental sepsis model of rats. Life sciences, 221, 327–334. https://doi.org/10.1016/j.lfs.2019.02.039
  • El-Demerdash, F. M., Baghdadi, H. H., Ghanem, N. F., & Mhanna, A. B. A. (2020). Nephroprotective role of bromelain against oxidative injury induced by aluminium in rats. Environmental toxicology and pharmacology, 80, 103509. https://doi.org/10.1016/j.etap.2020.103509
  • Eraslan, E., Tanyeli, A., Akdemir, F. N. E., & Güler, M. C. (2020). Reno-Protective Effects of 6-Shogaol on Kidney Tissue in Cecal Ligation and Puncture-Induced Polymicrobial Sepsis Rat Model. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(3), 244-250.
  • Ferah Okkay, I., Okkay, U., Bayram, C., Cicek, B., Sezen, S., Aydin, I. C., Mendil, A. S., & Hacimuftuoglu, A. (2023). Bromelain protects against cisplatin-induced ocular toxicity through mitigating oxidative stress and inflammation. Drug and chemical toxicology, 46(1), 69–76. https://doi.org/10.1080/01480545.2021.2011308
  • Gerin, F., Sener, U., Erman, H., Yilmaz, A., Aydin, B., Armutcu, F., & Gurel, A. (2016). The Effects of Quercetin on Acute Lung Injury and Biomarkers of Inflammation and Oxidative Stress in the Rat Model of Sepsis. Inflammation, 39(2), 700–705. https://doi.org/10.1007/s10753-015-0296-9
  • Giustina, A. D., Danielski, L. G., Novochadlo, M. M., Goldim, M. P. S., Joaquim, L., Metzker, K. L. L., Carli, R. J., Denicol, T., Cidreira, T., Vieira, T., & Petronilho, F. (2019). Vitamin B6 reduces oxidative stress in lungs and liver in experimental sepsis. Anais da Academia Brasileira de Ciencias, 91(4), e20190434. https://doi.org/10.1590/0001-3765201920190434
  • Gur, C., Kandemir, F. M., Caglayan, C., & Satıcı, E. (2022). Chemopreventive effects of hesperidin against paclitaxel-induced hepatotoxicity and nephrotoxicity via amendment of Nrf2/HO-1 and caspase-3/Bax/Bcl-2 signaling pathways. Chemico-biological interactions, 365, 110073. https://doi.org/10.1016/j.cbi.2022.110073
  • Cihan, G. Ü. R., Kandemir, Ö., & Kandemir, F. M. (2022). Ratlarda diklofenak ile indüklenen kardiyotoksisite üzerine krisinin etkilerinin oksidatif stres, endoplazmik retikulum stresi ve apoptoz belirteçleri ile değerlendirilmesi. Kocatepe Veterinary Journal, 15(2), 151-160.
  • Gur, C., Akarsu, S. A., Akaras, N., Tuncer, S. C., & Kandemir, F. M. (2023). Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite-induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environmental toxicology, 38(6), 1265–1276. https://doi.org/10.1002/tox.23762
  • Hu, Q., Wang, Q., Han, C., & Yang, Y. (2020). Sufentanil attenuates inflammation and oxidative stress in sepsis-induced acute lung injury by downregulating KNG1 expression. Molecular medicine reports, 22(5), 4298–4306. https://doi.org/10.3892/mmr.2020.11526
  • Kandemir, F. M., Caglayan, C., Aksu, E. H., Yildirim, S., Kucukler, S., Gur, C., & Eser, G. (2020). Protective effect of rutin on mercuric chloride-induced reproductive damage in male rats. Andrologia, 52(3), e13524. https://doi.org/10.1111/and.13524
  • Kandemir, F. M., Ileriturk, M., & Gur, C. (2022). Rutin protects rat liver and kidney from sodium valproate-induce damage by attenuating oxidative stress, ER stress, inflammation, apoptosis and autophagy. Molecular biology reports, 49(7), 6063–6074. https://doi.org/10.1007/s11033-022-07395-0
  • Khazaeel, K., Rad, O. R., Jamshidian, J., Tabandeh, M. R., Mohammadi, G., & Atashfaraz, A. (2022). Effect of bromelain on sperm quality, testicular oxidative stress and expression of oestrogen receptors in bisphenol-A treated male mice. Andrologia, 54(11), e14584. https://doi.org/10.1111/and.14584
  • Lawrence, R. A., & Burk, R. F. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and biophysical research communications, 71(4), 952–958. https://doi.org/10.1016/0006-291x(76)90747-6
  • Li, L., Wang, H., Zhao, S., Zhao, Y., Chen, Y., Zhang, J., Wang, C., Sun, N., & Fan, H. (2022). Paeoniflorin ameliorates lipopolysaccharide-induced acute liver injury by inhibiting oxidative stress and inflammation via SIRT1/FOXO1a/SOD2 signaling in rats. Phytotherapy research: PTR, 36(6), 2558–2571. https://doi.org/10.1002/ptr.7471
  • Li, R., Ren, T., & Zeng, J. (2019). Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3K/Akt/GSK-3β/mTOR Pathway in Rats. BioMed research international, 2019, 5240898. https://doi.org/10.1155/2019/5240898
  • Liu, Y., Mu, S., Li, X., Liang, Y., Wang, L., & Ma, X. (2019). Unfractionated Heparin Alleviates Sepsis-Induced Acute Lung Injury by Protecting Tight Junctions. The Journal of surgical research, 238, 175–185. https://doi.org/10.1016/j.jss.2019.01.020
  • Liu, Y., Zhang, Y., Feng, Q., Liu, Q., Xie, J., Li, H., Yang, F., Liu, X., Gao, W., Bai, X., Li, Z., & Wang, Y. (2021). GPA Peptide Attenuates Sepsis-Induced Acute Lung Injury in Mice via Inhibiting Oxidative Stress and Pyroptosis of Alveolar Macrophage. Oxidative medicine and cellular longevity, 2021, 5589472. https://doi.org/10.1155/2021/5589472
  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. The Journal of biological chemistry, 193(1), 265–275.
  • Margotti, W., Goldim, M. P. S., Machado, R. S., Bagio, E., Dacoregio, C., Bernades, G., Lanzzarin, E., Stork, S., Cidreira, T., Denicol, T. L., Joaquim, L., Danielski, L. G., Metzker, K. L. L., Bonfante, S., Margotti, E., & Petronilho, F. (2022). Oxidative stress in multiple organs after sepsis in elderly rats. Experimental gerontology, 160, 111705. https://doi.org/10.1016/j.exger.2022.111705
  • Petronilho, F., Florentino, D., Danielski, L. G., Vieira, L. C., Martins, M. M., Vieira, A., Bonfante, S., Goldim, M. P., & Vuolo, F. (2016). Alpha-Lipoic Acid Attenuates Oxidative Damage in Organs After Sepsis. Inflammation, 39(1), 357–365. https://doi.org/10.1007/s10753-015-0256-4
  • Placer, Z. A., Cushman, L. L., & Johnson, B. C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical biochemistry, 16(2), 359–364. https://doi.org/10.1016/0003-2697(66)90167-9
  • Sedlak, J., & Lindsay, R. H. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical biochemistry, 25(1), 192–205. https://doi.org/10.1016/0003-2697(68)90092-4
  • Semis, H. S., Gur, C., Ileriturk, M., Kandemir, F. M., & Kaynar, O. (2022). Evaluation of Therapeutic Effects of Quercetin Against Achilles Tendinopathy in Rats via Oxidative Stress, Inflammation, Apoptosis, Autophagy, and Metalloproteinases. The American journal of sports medicine, 50(2), 486–498. https://doi.org/10.1177/03635465211059821
  • Simsek, H., & Akaras, N. (2023). Acacetin ameliorates acetylsalicylic acid-induced gastric ulcer in rats by interfering with oxidative stress, inflammation, and apoptosis. International Journal of Medical Biochemistry, 6(2).
  • Sun, Y., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 34(3), 497–500.
  • Şehirli, A. Ö., Sayiner, S., Savtekin, G., & Velioğlu-Öğünç, A. (2021). Protective effect of bromelain on corrosive burn in rats. Burns : journal of the International Society for Burn Injuries, 47(6), 1352–1358.
  • Şimşek, H., Akaras, N., Gür, C., Küçükler, S., & Kandemir, F. M. (2023). Beneficial effects of Chrysin on Cadmium-induced nephrotoxicity in rats: Modulating the levels of Nrf2/HO-1, RAGE/NLRP3, and Caspase-3/Bax/Bcl-2 signaling pathways. Gene, 875, 147502. Advance online publication. https://doi.org/10.1016/j.gene.2023.147502
  • Turk, E., Kandemir, F. M., Yildirim, S., Caglayan, C., Kucukler, S., & Kuzu, M. (2019). Protective Effect of Hesperidin on Sodium Arsenite-Induced Nephrotoxicity and Hepatotoxicity in Rats. Biological trace element research, 189(1), 95–108. https://doi.org/10.1007/s12011-018-1443-6
  • Yu, H., Liu, Y., Wang, M., Restrepo, R. J., Wang, D., Kalogeris, T. J., Neumann, W. L., Ford, D. A., & Korthuis, R. J. (2020). Myeloperoxidase instigates proinflammatory responses in a cecal ligation and puncture rat model of sepsis. American journal of physiology. Heart and circulatory physiology, 319(3), H705–H721. https://doi.org/10.1152/ajpheart.00440.2020
  • Zeng, M., He, W., Li, L., Li, B., Luo, L., Huang, X., Guan, K., & Chen, W. (2015). Ghrelin attenuates sepsis-associated acute lung injury oxidative stress in rats. Inflammation, 38(2), 683–690. https://doi.org/10.1007/s10753-014-9977-z
  • Zhang, J., Wang, C., Wang, H., Li, X., Xu, J., & Yu, K. (2021). Loganin alleviates sepsis-induced acute lung injury by regulating macrophage polarization and inhibiting NLRP3 inflammasome activation. International immunopharmacology, 95, 107529. https://doi.org/10.1016/j.intimp.2021.107529
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Histoloji ve Embriyolojisi
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Nurhan Akaras 0000-0002-8457-9448

Erdem Toktay 0000-0002-0715-2707

Hasan Şimşek 0000-0001-5573-4923

Nevra Aydemir Celep 0000-0003-1608-5881

Neslihan Yüce 0000-0001-6878-0646

Yayımlanma Tarihi 30 Eylül 2023
Kabul Tarihi 25 Temmuz 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 16 Sayı: 3

Kaynak Göster

APA Akaras, N., Toktay, E., Şimşek, H., Aydemir Celep, N., vd. (2023). Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model. Kocatepe Veterinary Journal, 16(3), 252-259. https://doi.org/10.30607/kvj.1302701
AMA Akaras N, Toktay E, Şimşek H, Aydemir Celep N, Yüce N. Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model. kvj. Eylül 2023;16(3):252-259. doi:10.30607/kvj.1302701
Chicago Akaras, Nurhan, Erdem Toktay, Hasan Şimşek, Nevra Aydemir Celep, ve Neslihan Yüce. “Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model”. Kocatepe Veterinary Journal 16, sy. 3 (Eylül 2023): 252-59. https://doi.org/10.30607/kvj.1302701.
EndNote Akaras N, Toktay E, Şimşek H, Aydemir Celep N, Yüce N (01 Eylül 2023) Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model. Kocatepe Veterinary Journal 16 3 252–259.
IEEE N. Akaras, E. Toktay, H. Şimşek, N. Aydemir Celep, ve N. Yüce, “Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model”, kvj, c. 16, sy. 3, ss. 252–259, 2023, doi: 10.30607/kvj.1302701.
ISNAD Akaras, Nurhan vd. “Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model”. Kocatepe Veterinary Journal 16/3 (Eylül 2023), 252-259. https://doi.org/10.30607/kvj.1302701.
JAMA Akaras N, Toktay E, Şimşek H, Aydemir Celep N, Yüce N. Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model. kvj. 2023;16:252–259.
MLA Akaras, Nurhan vd. “Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model”. Kocatepe Veterinary Journal, c. 16, sy. 3, 2023, ss. 252-9, doi:10.30607/kvj.1302701.
Vancouver Akaras N, Toktay E, Şimşek H, Aydemir Celep N, Yüce N. Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model. kvj. 2023;16(3):252-9.

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