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SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY

Yıl 2015, Cilt: 49 Sayı: 2, 1 - 7, 18.05.2015
https://doi.org/10.17096/jiufd.60136

Öz

Purpose: The aim of this study was to investigate the micro tensile bond strength of a self-etch adhesive system following 1 year storage in water.

Materials and Methods: 10 sound human molar teeth were used for micro tensile bond strength test. Two-step self-etch dentin adhesive (Clearfil SE Bond®) was applied to the flat dentin surfaces according to the manufacturer’s instructions. Composite blocks (Z-250; 3M ESPE) of 5 mm in height have been prepared by using layering technique. Teeth were stored in water for 24 hours at 37°C and longitudinally sectioned to obtain dentin sticks of 1 mm2.. Randomly selected samples from half of the teeth were immediately subjected to micro tensile test and). Remaining specimens were tested after 1 year storage in water. Bond strengths were calculated in megapascal (MPa).

Results: Means and standard deviations of the Clearfil SE Bond® micro tensile bond strength values were, respectively, 37.31 ± 13.77 MPa and 24.78 ± 2.99 MPa after 24 h and 1 year of storage in water. The difference was statistically significant (p=0,031).

Conclusion: Long-term storage in water decreased the micro tensile bond strength values of the two-step self-etch adhesive which has been accepted as the gold standard in bond strength tests.

Kaynakça

  • Sano H. Microtensile testing, nanoleakage, and biodegradation of resin-dentin bonds. J Dent Rest 2006;85(1):11-14.
  • De Munck J, Landuyt KV, PeumansM,Poitevin A, Lambrechts P, Braem M, Van Meerbeek B. A critical review of durability of adhesion to tooth tissue: Methods and results. J Dent Res 2005;84(2):118-132.
  • Tay FR, Pashley DH. Have dentin adhesives become too hydrophilic? J Can Dent Assoc 2003;69(11):726-731.
  • Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res 2000;79(6):1385-1391.
  • Amaral FL, Colucci V, Palma-Dibb RG, Corona SA. Assessment of in vitro methods used to promote adhesive interface degradation: a critical review. J Esthet Restor Dent 2007;19(6):340-354.
  • Yiu CK, King NM, Pashley DH, Suh BI, Carvalho RM, Carrilho MR, Tay F. Effect of resin hydrophilicity and water storage on resin strength. Biomaterials 2004;25(26):5789-5796.
  • Toledano M, Osorio R, Osorio E, Aguilera FS, Yamauti M, Pashley DH, Tay F. Durability of resin-dentin bonds: effects of direct/indirect exposure and storage media. Dent Mater 2007;23(7):885-892.
  • Cenci MS, Venturini D, Pereira-Cenci T, Piva E, Demarco FF. The effect of polishing techniques and time on the surface characteristics and sealing ability of resin composite restorations after one-year storage. Oper Dent 2008;33(2):169-176.
  • Mine A, De Munck J, Cardoso MV, Van Landuyt KL, Poitevin A, Kuboki T, Yoshida Y, Suzuki K, Lambrechts P, Van Meerbeek B. Bonding effectiveness of two contemporary self-etch adhesives to enamel and dentin. J Dent 2009;37(11):872-883.
  • Peumans M, De Munck D, Van Landuyt K, Lambrechts P, Van Meerbeek B. Five-year clinical effectiveness of a two-step self-etching adhesive. J Adhes Dent 2007;9(1):7-10.
  • Loguercio AD, Lorini E, Weiss RV, Tori AP, Picinatto CC, Ribeiro NR, Reis A. A 12-month clinical evaluation of composite resins in class III restorations. J Adhes Dent 2007;9(1):57–64.
  • Yuan Y, Shimada Y, Ichinose S, Tagami J. Effect of dentin depth on hybridization quality using different bonding tactics in vivo. J Dent 2007;35(8):664-672.
  • Van Meerbeek B, Peumans M, Poitevin A, Mine A, Van Ende A, Neves A, De Munck J. Relationship between bond-strength tests and clinical outcomes. Dent Mater 2010;26(2):100-121.
  • De Munck J, Van den Steen PE, Mine A, Van Landuyt KL, Poitevin A, Opdenakker G, Van Meerbeek B. Inhibition of enzymatic degradation of adhesive-dentin interfaces. J Dent Res 2009;88(12):1101-1106.
  • De Munck J, Shirai K, Yoshida Y, Inoue S, Van Landuyt K, Lambrechts P, Suzuki K, Shintani H, Van Meerbeek B. Effect of water storage on the bonding effectiveness of 6 adhesives to Class I cavity dentin. Oper Dent 2006;31(4):456-465.
  • Reis A, Albuquerque M, Pegoraro M, Mattei G, Bauer JR, Grande RH, Klein-Junior CA, Baumhardt-Neto R, Loguercio AD. Can the durability of one-step self-etch adhesives be improved by double application or by an extra layer of hydrophobic resin? J Dent 2008;36(5):309-315.
  • Mena-Serrano A, Garcia EJ, Loguercio AD, Reis A. Effect of sonic application mode on the resin-dentin bond strength and nanoleakage of simplified self-etch adhesive. Clin Oral Investig 2014;18(3):729-736.
  • Tekçe N, Demirci M, Tuncer S, Uysal Ö. Effect of Different Application Techniques of All-in-One Adhesives on Microtensile Bond Strength to Sound and Caries-Affected Dentin. J Adhesion 2015;91(4):245-261.
  • Shirai K, De Munck J, Yoshida Y, Inoue S, Lambrechts P, Suziki K, Shintani H, Van Meerbeek B. Effect of cavity configuration and aging on the bonding effectiveness of six adhesives to dentin. Dent Mater 2005;21(2):110-124.
  • Toledano M, Osorio R, Ceballos L, Fuentes MV, Fernandes CA, Tay FR, Carvalho RM. Microtensile bond strength of several adhesive systems to diffrent dentin depths. Am J Dent 2003;16(5):292-298.
  • Van Landuyt KL, Kanumilli P, De Munck J, Peumans M, Lambrechts P, Van Meerbeek B. Bond strength of a mild self-etch adhesive with and without prior acid-etching. J Dent 2006;34(1):77-85.
  • Proença JP, Polido M, Osorio E, Erhardt MC, Aguilera FS, Garcia-Godoy F, Osorio R, Toledano M. Dentin regional bond strength of self-etch and total-etch adhesive systems. Dent Mater 2007;23(12):1542-1548.
  • Sadek FT, Goracci C, Cardoso PE, Tay FR, Ferrari M. Microtensile bond strength of current dentin adhesives measured immediately and 24 hours after application. J Adhes Dent 2005;7(4):297-302.
  • Inoue S, Koshiro K, Yoshida Y, De Munck J, Nagakane K, Suzuki K, Sano H, Van Meerbeek B. Hydrolytic stability of self-etch adhesives bonded to dentin. J Dent Res 2005;84(12):1160-1164.
  • Kenshima S, Reis A, Uceda-Gomez N, Tancredo LL, Filho LE, Nogueira FN, Loguercio AD. Effect of smear layer thickness and pH of self-etching adhesive systems on the bond strength and gap formation to dentin. J Adhes Dent 2005;7(2):117-126.
  • Lodovici E, Reis A, Geraldeli S, Ferracane JL, Ballester RY, Filho LE. Does adhesive thickness affect resin-dentin bond strength after thermal/load cycling? Oper Dent 2009;34(1):58-64.
  • Yeşilyurt C, Bulucu B, Koyutürk A. Adezif sistemlerin derin ve yüzeyel dentine mikro-tensile bağlanma dayanımları. İstanbul Üniv Diş Hek Fak Derg 2007;41(4):13-20.
  • Van Landuyt KL, Mine A, De Munck J, Jaecques S, Peumans M, Lambrechts P, Van Meerbeek B. Are one-step adhesives easier to use and better performing? Multifactorial assessment of contemporary one-step self-etching adhesives. J Adhes Dent 2009;11(3):175-190.
  • Armstrong SR, Vargas MA, Fang Q, Laffoon JE. Microtensile bond strength of a total-etch 3-step, total-etch 2-step, self-etch 2-step, and a self-etch 1-step dentin bonding system through 15-month water storage. J Adhes Dent 2003;5(1):47-56.
  • Akın GE, Hergüner-Siso Ş, Akın H. Termal siklus ve suda bekletmenin kendinden asitli adezivlerin dentine mikrogerilim bağlanma dayanımları üzerine etkileri. Atatürk Üniv Dişhek Fak Derg 2012;22(2):125-131.
  • Torkabadi S, Nakajima M, Ikeda M, Foxton RM, Tagami J. Bonding durability of HEMA-free and HEMA-containing one-step adhesives to dentine surrounded by bonded enamel. J Dent, 2008;36(1):80-86.
  • Hashimoto M. A review- micromorphological evidence of degradation in resin-dentin bonds and potential preventional solutions. J Biomed Mater Res B Appl Biomater 2010;92(1):268-280.
  • Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: aging and stability of the bonded interface. Dent Mater 2008;24(1):90-101.
  • Hosaka K, Nakajima M, Takahashi M, Itoh S, Ikeda M, Tagami J, Pashley DH. Relationship between mechanical properties of one-step self-etch adhesives and water sorption. Dent Mater 2010;26(4):360-367.
  • Reis A, Grandi V, Carlotto L, Bortoli G, Patzlaff R, Rodrigues Accorite Mde L, Dourado Loguercio A. Effect of smear layer thickness and acidity of self-etching solutions on early and long term bond strength to dentin. J Dent 2005;33(7):549-559.
  • Reis A, Grande RH, Oliveira GM, Lopes GC, Loguercio AD. A 2-year evaluation of moisture on microtensile bond strength and nanoleakage. Dent Mater 2007;23(7):862-870.
  • Perdigao J, Munoz M, Sezinando A, Loque-Martinez I, Staichak R, Reis A, Loguercio A. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper Dent 2014;39(5):489-499.

SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY

Yıl 2015, Cilt: 49 Sayı: 2, 1 - 7, 18.05.2015
https://doi.org/10.17096/jiufd.60136

Öz

Purpose: The aim of this study was to investigate the micro tensile bond strength of a self-etch etch dentin bağlayıcı sistemin 1 yıl suda bekletme adhesive system following 1 year storage in water. sonucunda bağlanma dayanım değerlerindeki Materials and Methods: 10 sound human molar teeth were used for micro tensile bond strength test. Twostep self-etch dentin adhesive (Clearfil SE Bond®) was applied to the flat dentin surfaces according to the manufacturer’s instructions. Composite blocks (Z250; 3M ESPE) of 5 mm in height have been prepared by using layering technique. Teeth were stored in water for 24 hours at 37°C and longitudinally sectioned to obtain dentin sticks of 1 mm2.Randomly selected samples from half of the teeth were immediately subjected to micro tensile test and. Remaining seçilen 5 dişe ait çubuklar 24 saat sonra, kalan specimens were tested after 1 year storage in water. 5 dişten hazırlanan çubuklar ise 1 yıl 37°C suda Bond strengths were calculated in megapascal (MPa).Results: Means and standard deviations of the Elde edilen bağlanma dayanımı değerleri megapascal Clearfil SE Bond® micro tensile bond strength values were, respectively, 37.31 ± 13.77 MPa and 24.78 ± (MPa) olarak kayıt edilmiştir

Kaynakça

  • Sano H. Microtensile testing, nanoleakage, and biodegradation of resin-dentin bonds. J Dent Rest 2006;85(1):11-14.
  • De Munck J, Landuyt KV, PeumansM,Poitevin A, Lambrechts P, Braem M, Van Meerbeek B. A critical review of durability of adhesion to tooth tissue: Methods and results. J Dent Res 2005;84(2):118-132.
  • Tay FR, Pashley DH. Have dentin adhesives become too hydrophilic? J Can Dent Assoc 2003;69(11):726-731.
  • Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res 2000;79(6):1385-1391.
  • Amaral FL, Colucci V, Palma-Dibb RG, Corona SA. Assessment of in vitro methods used to promote adhesive interface degradation: a critical review. J Esthet Restor Dent 2007;19(6):340-354.
  • Yiu CK, King NM, Pashley DH, Suh BI, Carvalho RM, Carrilho MR, Tay F. Effect of resin hydrophilicity and water storage on resin strength. Biomaterials 2004;25(26):5789-5796.
  • Toledano M, Osorio R, Osorio E, Aguilera FS, Yamauti M, Pashley DH, Tay F. Durability of resin-dentin bonds: effects of direct/indirect exposure and storage media. Dent Mater 2007;23(7):885-892.
  • Cenci MS, Venturini D, Pereira-Cenci T, Piva E, Demarco FF. The effect of polishing techniques and time on the surface characteristics and sealing ability of resin composite restorations after one-year storage. Oper Dent 2008;33(2):169-176.
  • Mine A, De Munck J, Cardoso MV, Van Landuyt KL, Poitevin A, Kuboki T, Yoshida Y, Suzuki K, Lambrechts P, Van Meerbeek B. Bonding effectiveness of two contemporary self-etch adhesives to enamel and dentin. J Dent 2009;37(11):872-883.
  • Peumans M, De Munck D, Van Landuyt K, Lambrechts P, Van Meerbeek B. Five-year clinical effectiveness of a two-step self-etching adhesive. J Adhes Dent 2007;9(1):7-10.
  • Loguercio AD, Lorini E, Weiss RV, Tori AP, Picinatto CC, Ribeiro NR, Reis A. A 12-month clinical evaluation of composite resins in class III restorations. J Adhes Dent 2007;9(1):57–64.
  • Yuan Y, Shimada Y, Ichinose S, Tagami J. Effect of dentin depth on hybridization quality using different bonding tactics in vivo. J Dent 2007;35(8):664-672.
  • Van Meerbeek B, Peumans M, Poitevin A, Mine A, Van Ende A, Neves A, De Munck J. Relationship between bond-strength tests and clinical outcomes. Dent Mater 2010;26(2):100-121.
  • De Munck J, Van den Steen PE, Mine A, Van Landuyt KL, Poitevin A, Opdenakker G, Van Meerbeek B. Inhibition of enzymatic degradation of adhesive-dentin interfaces. J Dent Res 2009;88(12):1101-1106.
  • De Munck J, Shirai K, Yoshida Y, Inoue S, Van Landuyt K, Lambrechts P, Suzuki K, Shintani H, Van Meerbeek B. Effect of water storage on the bonding effectiveness of 6 adhesives to Class I cavity dentin. Oper Dent 2006;31(4):456-465.
  • Reis A, Albuquerque M, Pegoraro M, Mattei G, Bauer JR, Grande RH, Klein-Junior CA, Baumhardt-Neto R, Loguercio AD. Can the durability of one-step self-etch adhesives be improved by double application or by an extra layer of hydrophobic resin? J Dent 2008;36(5):309-315.
  • Mena-Serrano A, Garcia EJ, Loguercio AD, Reis A. Effect of sonic application mode on the resin-dentin bond strength and nanoleakage of simplified self-etch adhesive. Clin Oral Investig 2014;18(3):729-736.
  • Tekçe N, Demirci M, Tuncer S, Uysal Ö. Effect of Different Application Techniques of All-in-One Adhesives on Microtensile Bond Strength to Sound and Caries-Affected Dentin. J Adhesion 2015;91(4):245-261.
  • Shirai K, De Munck J, Yoshida Y, Inoue S, Lambrechts P, Suziki K, Shintani H, Van Meerbeek B. Effect of cavity configuration and aging on the bonding effectiveness of six adhesives to dentin. Dent Mater 2005;21(2):110-124.
  • Toledano M, Osorio R, Ceballos L, Fuentes MV, Fernandes CA, Tay FR, Carvalho RM. Microtensile bond strength of several adhesive systems to diffrent dentin depths. Am J Dent 2003;16(5):292-298.
  • Van Landuyt KL, Kanumilli P, De Munck J, Peumans M, Lambrechts P, Van Meerbeek B. Bond strength of a mild self-etch adhesive with and without prior acid-etching. J Dent 2006;34(1):77-85.
  • Proença JP, Polido M, Osorio E, Erhardt MC, Aguilera FS, Garcia-Godoy F, Osorio R, Toledano M. Dentin regional bond strength of self-etch and total-etch adhesive systems. Dent Mater 2007;23(12):1542-1548.
  • Sadek FT, Goracci C, Cardoso PE, Tay FR, Ferrari M. Microtensile bond strength of current dentin adhesives measured immediately and 24 hours after application. J Adhes Dent 2005;7(4):297-302.
  • Inoue S, Koshiro K, Yoshida Y, De Munck J, Nagakane K, Suzuki K, Sano H, Van Meerbeek B. Hydrolytic stability of self-etch adhesives bonded to dentin. J Dent Res 2005;84(12):1160-1164.
  • Kenshima S, Reis A, Uceda-Gomez N, Tancredo LL, Filho LE, Nogueira FN, Loguercio AD. Effect of smear layer thickness and pH of self-etching adhesive systems on the bond strength and gap formation to dentin. J Adhes Dent 2005;7(2):117-126.
  • Lodovici E, Reis A, Geraldeli S, Ferracane JL, Ballester RY, Filho LE. Does adhesive thickness affect resin-dentin bond strength after thermal/load cycling? Oper Dent 2009;34(1):58-64.
  • Yeşilyurt C, Bulucu B, Koyutürk A. Adezif sistemlerin derin ve yüzeyel dentine mikro-tensile bağlanma dayanımları. İstanbul Üniv Diş Hek Fak Derg 2007;41(4):13-20.
  • Van Landuyt KL, Mine A, De Munck J, Jaecques S, Peumans M, Lambrechts P, Van Meerbeek B. Are one-step adhesives easier to use and better performing? Multifactorial assessment of contemporary one-step self-etching adhesives. J Adhes Dent 2009;11(3):175-190.
  • Armstrong SR, Vargas MA, Fang Q, Laffoon JE. Microtensile bond strength of a total-etch 3-step, total-etch 2-step, self-etch 2-step, and a self-etch 1-step dentin bonding system through 15-month water storage. J Adhes Dent 2003;5(1):47-56.
  • Akın GE, Hergüner-Siso Ş, Akın H. Termal siklus ve suda bekletmenin kendinden asitli adezivlerin dentine mikrogerilim bağlanma dayanımları üzerine etkileri. Atatürk Üniv Dişhek Fak Derg 2012;22(2):125-131.
  • Torkabadi S, Nakajima M, Ikeda M, Foxton RM, Tagami J. Bonding durability of HEMA-free and HEMA-containing one-step adhesives to dentine surrounded by bonded enamel. J Dent, 2008;36(1):80-86.
  • Hashimoto M. A review- micromorphological evidence of degradation in resin-dentin bonds and potential preventional solutions. J Biomed Mater Res B Appl Biomater 2010;92(1):268-280.
  • Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: aging and stability of the bonded interface. Dent Mater 2008;24(1):90-101.
  • Hosaka K, Nakajima M, Takahashi M, Itoh S, Ikeda M, Tagami J, Pashley DH. Relationship between mechanical properties of one-step self-etch adhesives and water sorption. Dent Mater 2010;26(4):360-367.
  • Reis A, Grandi V, Carlotto L, Bortoli G, Patzlaff R, Rodrigues Accorite Mde L, Dourado Loguercio A. Effect of smear layer thickness and acidity of self-etching solutions on early and long term bond strength to dentin. J Dent 2005;33(7):549-559.
  • Reis A, Grande RH, Oliveira GM, Lopes GC, Loguercio AD. A 2-year evaluation of moisture on microtensile bond strength and nanoleakage. Dent Mater 2007;23(7):862-870.
  • Perdigao J, Munoz M, Sezinando A, Loque-Martinez I, Staichak R, Reis A, Loguercio A. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper Dent 2014;39(5):489-499.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırmalar / Original Articles
Yazarlar

Safa Tuncer

Neslihan Tekçe

Dial Pashaev Bu kişi benim

Mustafa Demirci Bu kişi benim

Canan Baydemir

Yayımlanma Tarihi 18 Mayıs 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 49 Sayı: 2

Kaynak Göster

APA Tuncer, S., Tekçe, N., Pashaev, D., Demirci, M., vd. (2015). SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY. Journal of Istanbul University Faculty of Dentistry, 49(2), 1-7. https://doi.org/10.17096/jiufd.60136
AMA Tuncer S, Tekçe N, Pashaev D, Demirci M, Baydemir C. SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY. J Istanbul Univ Fac Dent. Mayıs 2015;49(2):1-7. doi:10.17096/jiufd.60136
Chicago Tuncer, Safa, Neslihan Tekçe, Dial Pashaev, Mustafa Demirci, ve Canan Baydemir. “SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY”. Journal of Istanbul University Faculty of Dentistry 49, sy. 2 (Mayıs 2015): 1-7. https://doi.org/10.17096/jiufd.60136.
EndNote Tuncer S, Tekçe N, Pashaev D, Demirci M, Baydemir C (01 Mayıs 2015) SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY. Journal of Istanbul University Faculty of Dentistry 49 2 1–7.
IEEE S. Tuncer, N. Tekçe, D. Pashaev, M. Demirci, ve C. Baydemir, “SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY”, J Istanbul Univ Fac Dent, c. 49, sy. 2, ss. 1–7, 2015, doi: 10.17096/jiufd.60136.
ISNAD Tuncer, Safa vd. “SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY”. Journal of Istanbul University Faculty of Dentistry 49/2 (Mayıs 2015), 1-7. https://doi.org/10.17096/jiufd.60136.
JAMA Tuncer S, Tekçe N, Pashaev D, Demirci M, Baydemir C. SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY. J Istanbul Univ Fac Dent. 2015;49:1–7.
MLA Tuncer, Safa vd. “SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY”. Journal of Istanbul University Faculty of Dentistry, c. 49, sy. 2, 2015, ss. 1-7, doi:10.17096/jiufd.60136.
Vancouver Tuncer S, Tekçe N, Pashaev D, Demirci M, Baydemir C. SHORT- AND LONG-TERM BOND STRENGTHS OF A GOLD STANDARD TWO-STEP SELF-ETCH ADHESIVE SYSTEM TO DENTIN: A PRELIMINARY STUDY. J Istanbul Univ Fac Dent. 2015;49(2):1-7.