Effects of Surface Characteristics of Conventionally Manufactured, CAD/CAM Milled, and 3D-Printed Interim Materials on Adherence of Streptococcus Mutans and Candida Albicans

Simge Taşın; Meryem Güvenir; Artur Ismatullaev

doi:10.7126/cumudj.1228677

TR EN

Konvansiyonel, CAD/CAM frezeleme ve 3D baskı yöntemleriyle üretilmiş geçici materyallerin yüzey özelliklerinin Streptococcus mutans ve Candida albicans tutunumuna etkileri

Öz

Amaç: Bu çalışmanın amacı konvansiyonel, CAD/CAM frezeleme ve 3D baskı yöntemleriyle üretilmiş geçici materyallerinin Streptococcus mutans ve Candida albicans tutunumu duyarlılıklarına göre karşılaştırılması ve yüzey pürüzlülüğü ve hidrofobikliğin buna etkisinin incelenmesidir. Gereç ve Yöntemler: Otopolimerize polimetil metakrilat (A-PMMA), bis-akril kompozit (Bis-acrylate), CAD/CAM PMMA-bazlı polimer (Milled-PMMA) ve 3D baskı (Printed) rezinlerinden seksen adet disk şeklinde örnek üretildi. Örneklere 10,000 termal siklus (5-55°C) uygulandı ve kullanılan mikrobiyal süspansiyonlara göre örnekler iki gruba (n=10) ayrıldı: Streptococcus mutans ve Candida albicans. Örneklerin yüzey pürüzlülüğü (Ra) ve hidrofobikliği (WCA) ölçüldü. Örnekler Streptococcus mutans ve Candida albicans süspansiyonlarında 37°C'de 24 saat inkübe edilerek tutunum testi yapıldı ve yapışık hücreler koloni oluşturan birimler (CFU/ml) sayılarak değerlendirildi. Yüzeyleri analiz etmek için taramalı elektron mikroskobu (SEM) görüntüleri alındı (n=2). Veriler Kruskal-Wallis ve Mann-Whitney U testi ile analiz edildi. Ölçümler arasındaki korelasyonu belirlemek için Spearman korelasyon analizi kullanıldı (α=.05). Bulgular: Restoratif materyalin türü, Ra ve WCA'yı önemli ölçüde etkiledi. En yüksek Streptococcus mutans adezyonu Printed'de gözlemlenirken, ardından Bis-akrilat, A-PMMA ve Milled-PMMA’da gözlemlenmiştir (p=.001). En yüksek Candida albicans adezyonu A-PMMA'da kaydedilirken, ardından Printed, Bis-acrylate ve Milled-PMMA’da (r=.001) kaydedilmiştir. Streptococcus mutans (r=.660) ve Candida albicans (r=.413) tutunumu ve Ra arasında pozitif korelasyon gözlemlenmiştir. Örneklerin WCA'sı ile Streptococcus mutans tutunumu arasında negatif bir korelasyon bulunmuştur (r=-.373). Sonuçlar: Yüzey pürüzlülüğü mikroorganizmaların tutunumunda önemli rol oynar. CAD/CAM PMMA bazlı polimerler, uzun süreli kullanımda mikrobiyal yapışmayı azaltmak için daha iyi bir seçim olabilir.

Anahtar Kelimeler

Effects of Surface Characteristics of Conventionally Manufactured, CAD/CAM Milled, and 3D-Printed Interim Materials on Adherence of Streptococcus Mutans and Candida Albicans

Abstract

Objectives: The purpose of this in vitro study was to compare conventionally manufactured, CAD/CAM milled, and 3D-printed interim materials based on their susceptibility to adherence of Streptococcus mutans and Candida albicans, and examine the influence of surface roughness and hydrophobicity. Materials and Methods: Eighty disc-shaped specimens fabricated from autopolymerized polymethyl methacrylate (A-PMMA), bis-acryl composite (Bis-acrylate), CAD/CAM PMMA-based polymer (Milled-PMMA), and 3D-printed resin (Printed) were subjected to 10,000 thermal cycles (5-55 °C) and divided into two groups (n=10) according to microbial suspension used: Streptococcus mutans and Candida albicans. Surface roughness (Ra) and hydrophobicity (WCA) of specimens were measured. An adhesion test was performed by incubating the specimens in Streptococcus mutans and Candida albicans suspensions at 37 °C for 24 hours, and the adherent cells were evaluated by counting colony-forming units (CFU/ml). Scanning electron microscopy (SEM) was performed to analyze the surfaces (n=2). Data were analyzed with Kruskal-Wallis and Mann-Whitney U tests. Spearman’s correlation analysis was used to determine correlation among the measurements (α=.05). Results: Type of restorative material significantly influenced Ra and WCA. The highest adhesion of Streptococcus mutans was observed in Printed, followed by Bis-acrylate, A-PMMA, and Milled-PMMA (p=.001). The highest adhesion of Candida albicans was noted on A-PMMA, followed by Printed, Bis-acrylate, and Milled-PMMA (r=.001). The adhesion of Streptococcus mutans (r=.660) and Candida albicans (r=.413) showed a positive correlation with Ra. A negative correlation was found between WCA of the materials and Streptococcus mutans adhesion (r=-.373). Conclusions: Surface roughness plays an important role in the adherence of microorganisms. CAD/CAM PMMA-based polymers may be a better choice to reduce microbial adhesion in long-term use.

Keywords

Supporting Institution

Bu araştırma, kamu, ticari veya kar amacı gütmeyen sektörlerdeki finansman kuruluşlarından herhangi bir özel hibe almamıştır.

Project Number

Bu araştırma bir proje kapsamında yapılmamıştır.

Thanks

bu kısım boş bırakılacaktır

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Simge Taşın ^*
0000-0002-0483-2520
Türkiye

Meryem Güvenir
0000-0002-9702-9947
Türkiye

Artur Ismatullaev
0000-0002-0119-6821
Türkiye

Publication Date

September 29, 2023

Submission Date

January 4, 2023

Acceptance Date

July 2, 2023

Published in Issue

Year 2023 Volume: 26 Number: 3

DOI

https://doi.org/10.7126/cumudj.1228677

IZ

https://izlik.org/JA68GK36ED

Cite

RIS / Bibtex

EndNote

Taşın S, Güvenir M, Ismatullaev A (September 1, 2023) Effects of Surface Characteristics of Conventionally Manufactured, CAD/CAM Milled, and 3D-Printed Interim Materials on Adherence of Streptococcus Mutans and Candida Albicans. Cumhuriyet Dental Journal 26 3 227–234.

Cited By

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European Journal of Oral Sciences

https://doi.org/10.1111/eos.70027