Objectives: To evaluate the antimicrobial effects of experimental root canal medicaments on Enterococcus faecalis and Candida albicans on day 3 and day 7 using quantitative polymerase chain reaction (Q-PCR) analyses.
Materials and Methods: 240 single-rooted, single-canalled human teeth were used. Root canals were shaped mechanically and embedded in acrylic blocks, then sterilized in an autoclave. The samples were divided into two groups and infected with E. faecalis and C. albicans, and then divided into subgroups (n=10); calcium hydroxide (Ca(OH)2), triple paste (TAP), double paste (DP), modified double paste (MDP), lactoferrin (Lf), negative and positive groups. At the end of 3 and 7 days, paper points containing the root canal samples were placed in empty Eppendorf tubes, and DNA was isolated. Real-time Q-PCR was applied and the data were analyzed statistically.
Results: The antimicrobial effects of each medicine increased from days 3 to 7. Ca(OH)2 and TAP groups showed the similar eradication rates for E. faecalis and C. albicans on day 3 and 7 (P > 0.05). There was no significant difference between DP, MDP and Lf for the eradication of microorganisms at both experimental days (P > 0.05), except the amount of eradicated E. faecalis by DP at day 7 in which DP caught the similar percentages with TAP and Ca(OH)2 (P < 0.05).
Conclusions: Experimental pastes demonstrated antimicrobial efficiancy similar to those used routinely in endodontic clinic. It is promising that lactoferrin which is a very biocompatible material can be used in different combinations as an intracanal medicament.
Antibiotic paste Candida albicans Enterococcus faecalis Lactoferrin Root canal medicine
Marmara University Scientific Research Projects Committee
SAG-C-DRP 150513–0154
The authors would like to grateful for the financial support from Marmara University. The authors also thank Prof. Dr. Tanju Kadir and Dr. Banu Can.
SAG-C-DRP 150513–0154
Birincil Dil | İngilizce |
---|---|
Konular | Sağlık Kurumları Yönetimi |
Bölüm | Original Research Articles |
Yazarlar | |
Proje Numarası | SAG-C-DRP 150513–0154 |
Yayımlanma Tarihi | 15 Eylül 2021 |
Gönderilme Tarihi | 9 Nisan 2021 |
Yayımlandığı Sayı | Yıl 2021Cilt: 24 Sayı: 3 |
Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.
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