Öz
Objectives:
Eugenia caryophyllus has been used as a
traditional medicinal herb for thousands of years. It is used as an analgesic for tooth and toothache
with the effect of eugenol substance included in clove. Clove is used at toothpaste and some mouthwash. The
aim of this study was determined of in vitro cytotoxic effects Eugenia caryophyllus on fibroblast
cells.
Materials
and Methods: Cytotoxic
activity of Eugenia caryophyllus was evaluated
using XTT assay.
Results: In the present study, toxicity of
clove that is used as a pain reliever in dentistry has examined on L929 cells. Eugenia
caryophyllus were
incubated at 24 and 48 hours. The IC50 values of Eugenia caryophyllus (Clove) were found 57,92 ± 0,98 µg/mL in 24 h,
37,97 ± 0,74 µg/mL in 48h.
Conclusions: The acceptable level of cytotoxicity associated with Eugenia caryophyllus requires further evaluation to be used
Anahtar Kelimeler
Kaynakça
- 1. Toroğlu S. ve Çenet M. KSÜ Fen ve Mühendislik Dergisi. 2006; 9(2):12-20.
- 2. Hammer KA, Carson CF, Riley TV. Journal of Applied Microbiology.1999;86: 985-990.
- 3. Cortés-Rojas DF, Souza CRF, Oliveira WP. Clove (Syzygium aromaticum): a precious spice. Asian Pac J Trop Biomed. 2014;4(2): 90-96.
- 4. Cansian RL, Vanin AB, Orlando T, Piazza SP, Puton BM, Cardoso RI, Gonçalves IL, Honaiser TC, Paroul N, Oliveira D. Toxicity of clove essential oil and its ester eugenyl acetate against Artemia salina. Braz J Biol. 2017; 77(1):155-161. doi: 10.1590/1519-6984.12215.
- 5. Mahmoud H, Mina KA, Hassan R. Analgesic effect of clove essential oil in mice. Avi J Phytomed. 2011;1:1-6.
- 6. Gezegen H, Hepokur C, Tutar U, Ceylan M. Synthesis and Biological Evaluation of Novel 1-(4-(Hydroxy(1-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)phenyl)-3-phenylurea Derivatives. Chemistry Biodiversity. 2017;14 (10):doi: 10.1002/cbdv.201700223.
- 7. Heinrich M, Bremner P. Ethanobotany and ethanopharmacy-their role for anticancer drug development. Curr Drug Targets. 2006; 7: 239-245.
- 8. Thirumal M, Kishore G, Prithika R, Das S, Nithya G. In vitro anticancer activity of Tecoma stans (L) ethanolic leaf extract on human breast cancer. Journal of Pharmacognosy and Phytochemistry. 2016; 5(4): 331-334.
- 9. Jasna I, Suzana DB, Dusan M, Mihailo R, Irena Z. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. J Funct Food. 2013;5:416-423.
- 10. Nazrul IB, Jaripa MD, Nemai CN, Farhana A. Constituents of the essential oil from leaves and buds of clove (syzigium caryophyllatum L. Alston). African J Plant Sci. 2010;4:451–454.
- 11. Bressler K, Ron B. Effect Of Anesthetics On Stress And The Innate Immune System Of Gilthead Seabream (Sparus Surata), The Israeli. Journal of Aquaculture Bamidgeh.2014; 56(1):5-13.
- 12. Ho YC ,Huang FM, Chang YC. Mechanisms of cytotoxicity of eugenol in human osteoblastic cells in vitro.,ınternatıonal endodontic journal.2006; 39(5):389-393.
- 13. Trajano VN, Lima EO, Souza EL, Travassos AER. Inhibitory effect of the essential oil from Eugenia caryophyllata Thumb leaves on coalho cheese contaminating microorganisms. Ciência e Tecnologia de Alimentos. 2010;30(4):1001-1006.
- 14. Singh J, Baghotia A, Goel SP. Thunberg (Family Myrtaceae): a review.Eugenia caryophyllata International Journal of Research in Pharmaceutical and Biomedical Sciences. 2012;3(4):1469-1475.
- 15. Tragoolpua Y, Jatisatienr A. Anti‐herpes simplex virus activities of Eugenia caryophyllus(Spreng.) Bullock & S. G. Harrison and essential oil, eugenol. Phytotherapy research. 2017;21(12):1153-58.
- 16. Prashar A, Locke IC, Evans CS. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells, Cell Proliferation. 2006;39(4):241-248.
- 17. Machado M, Dinis AM, Salgueiro L, Custódio José BA, Cavaleiro C, Sousa MC. Anti-Giardia activity of Syzygium aromaticum essential oil and eugenol: Effects on growth, viability, adherence and ultrastructure. Exp Parasitol. 2011;127:732-739.
Öz
Amaç: Eugenia
caryophyllus binlerce yıldır
geleneksel şifalı bitki olarak kullanılmaktadır. Karanfil içerisindeki öjenol
maddesinin etkisi ile diş ve diş ağrıları için analjezik olarak kullanılır. Karanfil,
diş macunu ve bazı gargaralarda kullanılmaktadır. Bu çalışmanın amacı Eugenia caryophyllus in vitro sitotoksik
etkileri fibroblast hücre hatları üzerinde belirlemiştir.
Gereç ve Yöntem: Eugenia
caryophyllus'un
sitotoksik aktivitesi XTT testi kullanılarak değerlendirildi.
Bulgular: Bu çalışmada diş hekimliğinde ağrı kesici olarak
kullanılan karanfil toksisitesi L929 hücreleri üzerinde incelenmiştir. Eugenia caryophyllus 24 ve 48 saatlerde inkübe edildi. Eugenia
caryophyllus'un
(Karanfil) IC50 değerleri 24 saat de 37,92 ± 0,98 µg / mL, 48 saatte
de 37,97 ± 0,74 µg / mL olarak bulundu.
Sonuç: Eugenia caryophyllus ile ilişkili kabul edilebilir sitotoksisite seviyesi,
kullanılmak üzere daha fazla değerlendirme gerektirir.
Anahtar Kelimeler
Kaynakça
- 1. Toroğlu S. ve Çenet M. KSÜ Fen ve Mühendislik Dergisi. 2006; 9(2):12-20.
- 2. Hammer KA, Carson CF, Riley TV. Journal of Applied Microbiology.1999;86: 985-990.
- 3. Cortés-Rojas DF, Souza CRF, Oliveira WP. Clove (Syzygium aromaticum): a precious spice. Asian Pac J Trop Biomed. 2014;4(2): 90-96.
- 4. Cansian RL, Vanin AB, Orlando T, Piazza SP, Puton BM, Cardoso RI, Gonçalves IL, Honaiser TC, Paroul N, Oliveira D. Toxicity of clove essential oil and its ester eugenyl acetate against Artemia salina. Braz J Biol. 2017; 77(1):155-161. doi: 10.1590/1519-6984.12215.
- 5. Mahmoud H, Mina KA, Hassan R. Analgesic effect of clove essential oil in mice. Avi J Phytomed. 2011;1:1-6.
- 6. Gezegen H, Hepokur C, Tutar U, Ceylan M. Synthesis and Biological Evaluation of Novel 1-(4-(Hydroxy(1-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)phenyl)-3-phenylurea Derivatives. Chemistry Biodiversity. 2017;14 (10):doi: 10.1002/cbdv.201700223.
- 7. Heinrich M, Bremner P. Ethanobotany and ethanopharmacy-their role for anticancer drug development. Curr Drug Targets. 2006; 7: 239-245.
- 8. Thirumal M, Kishore G, Prithika R, Das S, Nithya G. In vitro anticancer activity of Tecoma stans (L) ethanolic leaf extract on human breast cancer. Journal of Pharmacognosy and Phytochemistry. 2016; 5(4): 331-334.
- 9. Jasna I, Suzana DB, Dusan M, Mihailo R, Irena Z. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. J Funct Food. 2013;5:416-423.
- 10. Nazrul IB, Jaripa MD, Nemai CN, Farhana A. Constituents of the essential oil from leaves and buds of clove (syzigium caryophyllatum L. Alston). African J Plant Sci. 2010;4:451–454.
- 11. Bressler K, Ron B. Effect Of Anesthetics On Stress And The Innate Immune System Of Gilthead Seabream (Sparus Surata), The Israeli. Journal of Aquaculture Bamidgeh.2014; 56(1):5-13.
- 12. Ho YC ,Huang FM, Chang YC. Mechanisms of cytotoxicity of eugenol in human osteoblastic cells in vitro.,ınternatıonal endodontic journal.2006; 39(5):389-393.
- 13. Trajano VN, Lima EO, Souza EL, Travassos AER. Inhibitory effect of the essential oil from Eugenia caryophyllata Thumb leaves on coalho cheese contaminating microorganisms. Ciência e Tecnologia de Alimentos. 2010;30(4):1001-1006.
- 14. Singh J, Baghotia A, Goel SP. Thunberg (Family Myrtaceae): a review.Eugenia caryophyllata International Journal of Research in Pharmaceutical and Biomedical Sciences. 2012;3(4):1469-1475.
- 15. Tragoolpua Y, Jatisatienr A. Anti‐herpes simplex virus activities of Eugenia caryophyllus(Spreng.) Bullock & S. G. Harrison and essential oil, eugenol. Phytotherapy research. 2017;21(12):1153-58.
- 16. Prashar A, Locke IC, Evans CS. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells, Cell Proliferation. 2006;39(4):241-248.
- 17. Machado M, Dinis AM, Salgueiro L, Custódio José BA, Cavaleiro C, Sousa MC. Anti-Giardia activity of Syzygium aromaticum essential oil and eugenol: Effects on growth, viability, adherence and ultrastructure. Exp Parasitol. 2011;127:732-739.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Original Research Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 17 Ekim 2018 |
| Gönderilme Tarihi | 17 Temmuz 2018 |
| Yayımlandığı Sayı | Yıl 2018Cilt: 21 Sayı: 3 |
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