Araştırma Makalesi
BibTex RIS Kaynak Göster

Yıl 2022, Cilt: 14 Sayı: 2, 24 - 29, 29.12.2022

İlhan Sabancılar

https://doi.org/10.56484/iamr.1162647

Öz

Kaynakça

  • 1- Sıegel Rl, Mıller Kd, Jemal A. Cancer statistics,. CA Cancer J Clin, 2016; 66:7–30.
  • 2- Das V, Kalıta J, Pal M. Predictive and prognostic biomarkers in colorectal cancer: A systematic review of recent advances and challenges. Biomedicine & Pharmacotherapy, (2017);87:8–19.
  • 3- Kehoe SI, Jayarao BM, Heinrichs AJ. A Survey of Bovine Colostrum Composition and Colostrum Management Practices on Pennsylvania Dairy farms. Journal of Medical Sci. 2007;90(9):4108–4116.
  • 4- Kleinsmith Ba. Bovıne Colostrum As A Resource For The Powerful Antıoxıdant Glutathıone. Journal of Medical Sci. 2009;13-25.
  • 5- Przybylska J, Albera E, Kankofer M. Antioxidants in Bovine Colostrum. Reprod Domest Anim. 2007;42(4):402–419.
  • 6- Kelly GS. Bovine Colostrums: A Review of Clinical Uses. Journal of Medical Sci. 2003;8(4):378–394.
  • 7- Lascelles AK. The İmmune System on The Ruminant Mammary Gland and its Role in The Control of Mastitis. Journal of Medical Sci. 1979;62(1):154–167.
  • 8- Argüello A, Castro N, Batista M, Moreno-Indias I, Morales-delaNuez A, Sanchez-Macias D. Chitotriosidase Activity in Goat Blood and Colostrum. Journal of Medical Sci. 2008;91(5):2067–2070.
  • 9- Erdem H, Atasever Ş. Yeni Doğan Buzağılarda Kolostrumun Önemi. Ondokuzmayıs Üniv Zir Fak Derg. 2005(2):79-84.
  • 10- Kittaneh M, Montero AJ, Glück S. Molecular Profiling for Breast Cancer: A Comprehensive Review. 2013;(5):61–70.
  • 11- Rossi A, Caracciolo V, Russo G, Reiss K, Giordano A. Medulloblastoma: from Molecular Pathology to Therapy. National Library of Medicine. 2008;14(4):971-986. 12- Karag C. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri ve Sağlık Üzerine Etkileri. Ege Üniversitesi Ziraat Fakültesi Derg. 2004;41(2):197–207. 13- Bounous G, Papenburg R, Kongshavn PA, Gold P, Fleiszer D. Dietary Whey Protein İnhibits The Development of Dimethylhydrazine İnduced Malignancy. Journal of Medical Sci. 1988;11(3):213–227.
  • 14- Fakharany E.M, Abu-serie M, Litus E.A, Permyakov S.E, Uversky V.N, Redwan E.M. The Use of Human, Cow, and Cmel Milk Albumins in Anticancer Comlexes with Oleic Asid. The Protein Journal, 2018;(37):203-215.
  • 15- McIntosh GH, Regester GO, Le Leu RK, Royle PJ. Smithers GW Dairy proteins protect against dimethylhydrazineinduced intestinal cancers in rats. Journal of Nutrition. 1995;(125):809–816.
  • 16- Karagözlü C, Bayerer M. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri Ve Sağlık Üzerine Etkileri. Ege Üniv. Ziraat Fak. Dergisi, 2004;41(2):197-207.

Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)

Yıl 2022, Cilt: 14 Sayı: 2, 24 - 29, 29.12.2022

İlhan Sabancılar

https://doi.org/10.56484/iamr.1162647

Öz

Abstract
Objective: It was aimed to determine the antitumor and antiproliferative activity of whey protein (whey) obtained from sheep colostrum isolation on colorectal cancer cells (Caco-2).
Method: Colostrum was separated into fractions as whey proteins using the isoelectric point. Before cell culture was studied, whey protein was sterilized by a membrane filter. Antitumoral activity levels of different concentrations of lyophilized proteins were measured by MTT viability test on cells and IC50 values were determined.
Results: Sheep whey proteins were incubated with Caco-2 cells for 24, 48 and 72 hours with serial dilutions starting with 3200 µg/ml and decreasing. When incubated with sheep whey Caco-2 cells, cell viability: 66.34% at 24 h; IC50 value of 8.414 µg/ml; IC50 value of 46.61% at 48 h was 5,838 µg/ml; The IC50 value of 4.61% at 72 h was found to be 4,741 µg/ml.
Conclusion: It was concluded that whey proteins obtained from sheep colostrum isolation have apoptotic and anticarcinogenic effects on Caco-2 cancer cell line and significantly inhibit the growth of tumor cells. We think that the results of this study will lead to studies to be carried out in vitro.

Anahtar Kelimeler

colorectal adenocarcinoma cytotoxicity whey proteins Colostrum

Kaynakça

  • 1- Sıegel Rl, Mıller Kd, Jemal A. Cancer statistics,. CA Cancer J Clin, 2016; 66:7–30.
  • 2- Das V, Kalıta J, Pal M. Predictive and prognostic biomarkers in colorectal cancer: A systematic review of recent advances and challenges. Biomedicine & Pharmacotherapy, (2017);87:8–19.
  • 3- Kehoe SI, Jayarao BM, Heinrichs AJ. A Survey of Bovine Colostrum Composition and Colostrum Management Practices on Pennsylvania Dairy farms. Journal of Medical Sci. 2007;90(9):4108–4116.
  • 4- Kleinsmith Ba. Bovıne Colostrum As A Resource For The Powerful Antıoxıdant Glutathıone. Journal of Medical Sci. 2009;13-25.
  • 5- Przybylska J, Albera E, Kankofer M. Antioxidants in Bovine Colostrum. Reprod Domest Anim. 2007;42(4):402–419.
  • 6- Kelly GS. Bovine Colostrums: A Review of Clinical Uses. Journal of Medical Sci. 2003;8(4):378–394.
  • 7- Lascelles AK. The İmmune System on The Ruminant Mammary Gland and its Role in The Control of Mastitis. Journal of Medical Sci. 1979;62(1):154–167.
  • 8- Argüello A, Castro N, Batista M, Moreno-Indias I, Morales-delaNuez A, Sanchez-Macias D. Chitotriosidase Activity in Goat Blood and Colostrum. Journal of Medical Sci. 2008;91(5):2067–2070.
  • 9- Erdem H, Atasever Ş. Yeni Doğan Buzağılarda Kolostrumun Önemi. Ondokuzmayıs Üniv Zir Fak Derg. 2005(2):79-84.
  • 10- Kittaneh M, Montero AJ, Glück S. Molecular Profiling for Breast Cancer: A Comprehensive Review. 2013;(5):61–70.
  • 11- Rossi A, Caracciolo V, Russo G, Reiss K, Giordano A. Medulloblastoma: from Molecular Pathology to Therapy. National Library of Medicine. 2008;14(4):971-986. 12- Karag C. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri ve Sağlık Üzerine Etkileri. Ege Üniversitesi Ziraat Fakültesi Derg. 2004;41(2):197–207. 13- Bounous G, Papenburg R, Kongshavn PA, Gold P, Fleiszer D. Dietary Whey Protein İnhibits The Development of Dimethylhydrazine İnduced Malignancy. Journal of Medical Sci. 1988;11(3):213–227.
  • 14- Fakharany E.M, Abu-serie M, Litus E.A, Permyakov S.E, Uversky V.N, Redwan E.M. The Use of Human, Cow, and Cmel Milk Albumins in Anticancer Comlexes with Oleic Asid. The Protein Journal, 2018;(37):203-215.
  • 15- McIntosh GH, Regester GO, Le Leu RK, Royle PJ. Smithers GW Dairy proteins protect against dimethylhydrazineinduced intestinal cancers in rats. Journal of Nutrition. 1995;(125):809–816.
  • 16- Karagözlü C, Bayerer M. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri Ve Sağlık Üzerine Etkileri. Ege Üniv. Ziraat Fak. Dergisi, 2004;41(2):197-207.
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Original Research Paper
Yazarlar

İlhan Sabancılar 0000-0002-0773-2752

Yayımlanma Tarihi 29 Aralık 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 14 Sayı: 2

Kaynak Göster

APA Sabancılar, İ. (2022). Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). International Archives of Medical Research, 14(2), 24-29. https://doi.org/10.56484/iamr.1162647
AMA Sabancılar İ. Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). IAMR. Aralık 2022;14(2):24-29. doi:10.56484/iamr.1162647
Chicago Sabancılar, İlhan. “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”. International Archives of Medical Research 14, sy. 2 (Aralık 2022): 24-29. https://doi.org/10.56484/iamr.1162647.
EndNote Sabancılar İ (01 Aralık 2022) Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). International Archives of Medical Research 14 2 24–29.
IEEE İ. Sabancılar, “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”, IAMR, c. 14, sy. 2, ss. 24–29, 2022, doi: 10.56484/iamr.1162647.
ISNAD Sabancılar, İlhan. “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”. International Archives of Medical Research 14/2 (Aralık 2022), 24-29. https://doi.org/10.56484/iamr.1162647.
JAMA Sabancılar İ. Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). IAMR. 2022;14:24–29.
MLA Sabancılar, İlhan. “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”. International Archives of Medical Research, c. 14, sy. 2, 2022, ss. 24-29, doi:10.56484/iamr.1162647.
Vancouver Sabancılar İ. Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). IAMR. 2022;14(2):24-9.
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