Evaluation of the Effects of Topical Vitamin D on Osseointegration of Titanium Implants: An Experimental Study
Öz
Background: The aim of this study was to evaluate the effects of topical vitamin D to on osseointegration when applied to the surface of titanium implants.
Material and Metods: Twenty male Spraque-Dawley rats were used in this study in a 4-week experimental period. 4 rats died during the experiments. The rats were divided into 2 groups: a test group and control group. Sixteen sandblasted and acid-etched implants were integrated surgically in the metaphyseal part of rat femurs after patella dissection. Prior to integration, the implants, which were 3.2 mm in diameter and 8 mm in length, were submerged in a 10% vitamin D solution in the test group. No treatment was applied in the control group. After the 4-week experimental period, the rats were sacrificed, and the implants were removed with surrounding bone tissue. Specimens were fixed for 7 days in a 10% formaldehyde solution. Bone implant contact (BIC) ratios were determined after using non-decalcified histological methods.
Results: In the experiment, 2 samples were removed because they were not suitable. No statistically significant differences were found for BIC ratios between the test (n = 7) and control group (n = 7) implants (P> 0.05).
Conclusion: It was concluded, within the limitations of this animal study, that topical vitamin D application on implant surfaces does not affect BIC ratios.
Anahtar Kelimeler
osseointegration bone implant contact topical vitamin-d rat femoral bone
Teşekkür
All of the experimental and surgical protocols in our study were carried out in the Experimental Research Center of Fırat University. In addition, ethical approval permission was given by Fırat University Animal Experiments Local Ethics Committee. The animals used in the study were provided by Fırat University Experimental Research Center. The authors wish to thanks (Implants Dental Implant System, AGS Medical, Istanbul, Turkey) for manufacturing and providing the titanium implants. The information presented in this study was presented as an oral presentation at the Turkish Dental Association 24th International Congress of Dentistry between 27-30 September 2018.
Kaynakça
- Lantto A, Lundqvist R, Wårdh I. Oral Status and Prosthetic Treatment Needs in Functionally Impaired and Elderly Individuals. Int J Prosthodont. 2017 Nov 22. doi: 10.11607/ijp.5491. [Epub ahead of print]
- Ayub KV, Ayub EA, Lins do Valle A, Bonfante G, Pegoraro T, Fernando L. Seven-Year Follow-up of Full-Arch Prostheses Supported by Four Implants: A Prospective Study. Int J Oral Maxillofac Implants. 2017 Nov/Dec;32(6):1351-1358.
- Dundar S, Yaman F, Saybak A, et.al. Evaluation of Effects of Topical Melatonin Application on Osseointegration of Dental Implant: An Experimental Study. J Oral Implantol 2016;42(5):386-389.
- Le Guehennec L, Goyenvalle E, Lopez-Heredia M-A, et.al. Histomorphometric analysis of the osseointegration of four different implant surfaces in the femoral epiphyses of rabbits. Clin Oral Impl Res 2008; 19:1103–1110.
- Rupp F, Liang L, Geis-Gerstorfer J, Scheideler L, Hüttig F. Surface characteristics of dental implants: A review. Dent Mater. 2018 Jan;34(1):40-57.
- Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dental Materials 2007;23: 844–854.
- Buser D, Schenk RK, Steinemann S, et.al. Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs. J Biomed Mater Res 1991;25:889-902.
- Albrektsson T, Wennerberg A. Oral implant surfaces: Part 1–review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont 2004;17:536–543.
- Dundar S, Yaman F, Gecor O, et. al. Effects of Local and Systemic Zoledronic Acid Application on Titanium Implant Osseointegration: An Experimental Study Conducted on Two Surface Types. J Craniofac Surg 2017;28(4):935-938.
- Abduljabbar T, Kellesarian SV, Vohra F, Akram Z, Kotsakis GA, Yunker M, et. al. Effect of Growth Hormone Supplementation on Osseointegration: A Systematic Review and Meta-analyses. Implant Dent. 2017 Aug;26(4):613-620.
- Wang T, Muhetaer H, Li J. Experimental study of transforming growth factor-β3 combined with dental pulp stem cells in promoting the implant's osseointegration. Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 Jun 9;52(6):367-373.
- Apostu D, Lucaciu O, Lucaciu GD, Crisan B, Crisan L, Baciut M, et. al. Systemic drugs that influence titanium implant osseointegration. Drug Metab Rev. 2017 Feb;49(1):92-104.
- Salomó-Coll O, Maté-Sánchez de Val JE, Ramírez-Fernandez MP, Hernández-Alfaro F, Gargallo-Albiol J, Calvo-Guirado JL. Topical applications of vitamin D on implant surface for bone-to-implant contact enhance: a pilot study in dogs part II. Clin Oral Implants Res. 2016 Jul;27(7):896-903.
- Javed F, Malmstrom H, Kellesarian SV, Al-Kheraif AA, Vohra F, Romanos GE. Efficacy of Vitamin D3 Supplementation on Osseointegration of Implants. Implant Dent. 2016 Apr;25(2):281-7.
- Liu W, Zhang S, Zhao D, Zou H, Sun N, Liang X, et. al. Vitamin D supplementation enhances the fixation of titanium implants in chronic kidney disease mice. PLoS One. 2014 Apr 21;9(4):e95689.
- Urbanski W, Marycz K, Krzak J, Pezowicz C, Dragan SF. Cytokine induction of sol-gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur. Int J Nanomedicine 2017;12:1639-1645.
- Ying G, Bo L, Yanjun J, Lina W, Binquan W. Effect of a local, one time, low-dose injection of zoledronic acid on titanium implant osseointegration in ovariectomized rats. Arch Med Sci 2016;12(5):941-949.
- Donath K, Breuner G. A method for the study of undecalcified bones and teeth with attached soft tissues. The Sage-Schliff (sawing and grinding) technique. J Oral Pathol 1982;11:318–326.
- Naito Y, Jimbo R, Bryington MS, Vandeweghe S, Chrcanovic BR, Tovar N, et. al. The influence of 1α.25-dihydroxyvitamin d3 coating on implant osseointegration in the rabbit tibia. J Oral Maxillofac Res. 2014 Oct 1;5(3):e3.
- Alhilou A, Do T, Mizban L, Clarkson BH, Wood DJ, Katsikogianni MG. Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating. ACS Omega. 2016 Aug 31;1(2):264-276.
- Mihailescu N, Stan GE, Duta L, Chifiriuc MC, Bleotu C, Sopronyi M, et. al. Structural, compositional, mechanical characterization and biological assessment of bovine-derived hydroxyapatite coatings reinforced with MgF2 or MgO for implants functionalization. Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:863-874.
- Lavos-Valereto IC, Wolynec S, Deboni MC, König B Jr. In vitro and in vivo biocompatibility testing of Ti-6Al-7Nb alloy with and without plasma-sprayed hydroxyapatite coating. J Biomed Mater Res. 2001;58(6):727-33.
- Eliaz N, Metoki N. Calcium Phosphate Bioceramics: A Review of Their History, Structure, Properties, Coating Technologies and Biomedical Applications. Materials (Basel). 2017 Mar 24;10(4). pii: E334.
- Cho YJ, Heo SJ, Koak JY, Kim SK, Lee SJ, Lee JH. Promotion of osseointegration of anodized titanium implants with a 1α,25-dihydroxyvitamin D3 submicron particle coating. Int J Oral Maxillofac Implants. 2011 Nov-Dec;26(6):1225-32.
- Fügl A, Gruber R, Agis H, Lzicar H, Keibl C, Schwarze UY, et. al. Alveolar bone regeneration in response to local application of calcitriol in vitamin D deficient rats. J Clin Periodontol. 2015 Jan;42(1):96-103.
- Salomó-Coll O, Maté-Sánchez de Val JE, Ramírez-Fernandez MP, Hernández-Alfaro F, Gargallo-Albiol J, Calvo-Guirado JL. Topical applications of vitamin D on implant surface for bone-to-implant contact enhance: a pilot study in dogs part II. Clin Oral Implants Res. 2016 Jul;27(7):896-903.
Topikal D Vitamininin Titanyum İmplantların Osseointegrasyonu Üzerine Etkilerinin Değerlendirilmesi: Deneysel Bir Çalışma
Öz
Amaç: Bu çalışmanın amacı, titanyum implantların yüzeyine uygulandığında topikal D vitamininin osseointegrasyon üzerindeki etkilerini değerlendirmektir.
Materyal ve metod: Bu çalışmada 4 haftalık bir deney periyodunda 20 erkek Spraque-Dawley sıçanı kullanıldı. 4 rat deneyler sırasında öldü. Daha sonra ratlar 2 gruba ayrıldı: Test grubu (n=8) ve kontrol grubu (n=8). Patella diseksiyonu sonrası sıçan femurlarının metafiz kısmına 16 tane kumlanmış ve asitle pürüzlendirilmiş implant cerrahi olarak entegre edildi. Entegrasyon öncesinde 3,2 mm çapında ve 8 mm uzunluğunda olan implantlar, test grubunda % 10'luk bir D vitamini çözeltisine daldırıldı. Kontrol grubuna herhangi bir tedavi uygulanmadı. 4 haftalık deney süresinden sonra, sıçanlar öldürüldü ve implantlar çevreleyen kemik dokusuyla birlikte çıkarıldı. Örnekler % 10 formaldehit solüsyonunda 7 gün fikse edildi. Kemik-implant kaynaşması (BIC) oranları, dekalsifiye edilmemiş histolojik yöntemler kullanılarak belirlendi.
Bulgular: Deneyde 2 örnek uygun olmadığı için çıkartıldı.Test (n=7) ve kontrol grubu (n=7) implantları arasında BIC oranları için istatistiksel olarak önemli farklılıklar tespit edilmedi (P> 0.05).
Sonuç: Bu hayvan çalışmasının sınırları dahilinde, implant yüzeylerine topikal D vitamini uygulamasının BIC oranlarını etkilemediği sonucuna varıldı.
Anahtar Kelimeler
osseointegrasyon kemik-implant kaynaşması topikal vitami-d sıçan femoral kemiği
Kaynakça
- Lantto A, Lundqvist R, Wårdh I. Oral Status and Prosthetic Treatment Needs in Functionally Impaired and Elderly Individuals. Int J Prosthodont. 2017 Nov 22. doi: 10.11607/ijp.5491. [Epub ahead of print]
- Ayub KV, Ayub EA, Lins do Valle A, Bonfante G, Pegoraro T, Fernando L. Seven-Year Follow-up of Full-Arch Prostheses Supported by Four Implants: A Prospective Study. Int J Oral Maxillofac Implants. 2017 Nov/Dec;32(6):1351-1358.
- Dundar S, Yaman F, Saybak A, et.al. Evaluation of Effects of Topical Melatonin Application on Osseointegration of Dental Implant: An Experimental Study. J Oral Implantol 2016;42(5):386-389.
- Le Guehennec L, Goyenvalle E, Lopez-Heredia M-A, et.al. Histomorphometric analysis of the osseointegration of four different implant surfaces in the femoral epiphyses of rabbits. Clin Oral Impl Res 2008; 19:1103–1110.
- Rupp F, Liang L, Geis-Gerstorfer J, Scheideler L, Hüttig F. Surface characteristics of dental implants: A review. Dent Mater. 2018 Jan;34(1):40-57.
- Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dental Materials 2007;23: 844–854.
- Buser D, Schenk RK, Steinemann S, et.al. Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs. J Biomed Mater Res 1991;25:889-902.
- Albrektsson T, Wennerberg A. Oral implant surfaces: Part 1–review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont 2004;17:536–543.
- Dundar S, Yaman F, Gecor O, et. al. Effects of Local and Systemic Zoledronic Acid Application on Titanium Implant Osseointegration: An Experimental Study Conducted on Two Surface Types. J Craniofac Surg 2017;28(4):935-938.
- Abduljabbar T, Kellesarian SV, Vohra F, Akram Z, Kotsakis GA, Yunker M, et. al. Effect of Growth Hormone Supplementation on Osseointegration: A Systematic Review and Meta-analyses. Implant Dent. 2017 Aug;26(4):613-620.
- Wang T, Muhetaer H, Li J. Experimental study of transforming growth factor-β3 combined with dental pulp stem cells in promoting the implant's osseointegration. Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 Jun 9;52(6):367-373.
- Apostu D, Lucaciu O, Lucaciu GD, Crisan B, Crisan L, Baciut M, et. al. Systemic drugs that influence titanium implant osseointegration. Drug Metab Rev. 2017 Feb;49(1):92-104.
- Salomó-Coll O, Maté-Sánchez de Val JE, Ramírez-Fernandez MP, Hernández-Alfaro F, Gargallo-Albiol J, Calvo-Guirado JL. Topical applications of vitamin D on implant surface for bone-to-implant contact enhance: a pilot study in dogs part II. Clin Oral Implants Res. 2016 Jul;27(7):896-903.
- Javed F, Malmstrom H, Kellesarian SV, Al-Kheraif AA, Vohra F, Romanos GE. Efficacy of Vitamin D3 Supplementation on Osseointegration of Implants. Implant Dent. 2016 Apr;25(2):281-7.
- Liu W, Zhang S, Zhao D, Zou H, Sun N, Liang X, et. al. Vitamin D supplementation enhances the fixation of titanium implants in chronic kidney disease mice. PLoS One. 2014 Apr 21;9(4):e95689.
- Urbanski W, Marycz K, Krzak J, Pezowicz C, Dragan SF. Cytokine induction of sol-gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur. Int J Nanomedicine 2017;12:1639-1645.
- Ying G, Bo L, Yanjun J, Lina W, Binquan W. Effect of a local, one time, low-dose injection of zoledronic acid on titanium implant osseointegration in ovariectomized rats. Arch Med Sci 2016;12(5):941-949.
- Donath K, Breuner G. A method for the study of undecalcified bones and teeth with attached soft tissues. The Sage-Schliff (sawing and grinding) technique. J Oral Pathol 1982;11:318–326.
- Naito Y, Jimbo R, Bryington MS, Vandeweghe S, Chrcanovic BR, Tovar N, et. al. The influence of 1α.25-dihydroxyvitamin d3 coating on implant osseointegration in the rabbit tibia. J Oral Maxillofac Res. 2014 Oct 1;5(3):e3.
- Alhilou A, Do T, Mizban L, Clarkson BH, Wood DJ, Katsikogianni MG. Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating. ACS Omega. 2016 Aug 31;1(2):264-276.
- Mihailescu N, Stan GE, Duta L, Chifiriuc MC, Bleotu C, Sopronyi M, et. al. Structural, compositional, mechanical characterization and biological assessment of bovine-derived hydroxyapatite coatings reinforced with MgF2 or MgO for implants functionalization. Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:863-874.
- Lavos-Valereto IC, Wolynec S, Deboni MC, König B Jr. In vitro and in vivo biocompatibility testing of Ti-6Al-7Nb alloy with and without plasma-sprayed hydroxyapatite coating. J Biomed Mater Res. 2001;58(6):727-33.
- Eliaz N, Metoki N. Calcium Phosphate Bioceramics: A Review of Their History, Structure, Properties, Coating Technologies and Biomedical Applications. Materials (Basel). 2017 Mar 24;10(4). pii: E334.
- Cho YJ, Heo SJ, Koak JY, Kim SK, Lee SJ, Lee JH. Promotion of osseointegration of anodized titanium implants with a 1α,25-dihydroxyvitamin D3 submicron particle coating. Int J Oral Maxillofac Implants. 2011 Nov-Dec;26(6):1225-32.
- Fügl A, Gruber R, Agis H, Lzicar H, Keibl C, Schwarze UY, et. al. Alveolar bone regeneration in response to local application of calcitriol in vitamin D deficient rats. J Clin Periodontol. 2015 Jan;42(1):96-103.
- Salomó-Coll O, Maté-Sánchez de Val JE, Ramírez-Fernandez MP, Hernández-Alfaro F, Gargallo-Albiol J, Calvo-Guirado JL. Topical applications of vitamin D on implant surface for bone-to-implant contact enhance: a pilot study in dogs part II. Clin Oral Implants Res. 2016 Jul;27(7):896-903.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 27 Ağustos 2021 |
| Gönderilme Tarihi | 25 Mart 2021 |
| Kabul Tarihi | 25 Mayıs 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 18 Sayı: 2 |
Harran Üniversitesi Tıp Fakültesi Dergisi / Journal of Harran University Medical Faculty