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Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells

Yıl 2018, Cilt: 45 Sayı: 4, 447 - 461, 13.12.2018
https://doi.org/10.5798/dicletip.497923

Öz

Objective: Purpose
of the study was to research the effect of an electromagnetic field created by
a high voltage line on the testes tissue and the serum biochemistry of Wistar
albino male rats. At the same time, in the study also examined the protective
effects of melatonin and ganoderma lucidum.



Methods: In
the study, 64 rats were divided into eight equal groups (n: 8). In experiments
lasting 26 and 52 days, the following groups were used: High voltage (HV), HV +
Ganoderma lucidum (GI), HV + Melatonin (MEL), and Sham-control. MEL (10 mg/kg)
was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely
low frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied
for eight hours per day.



Results:
The biochemical results of the study show an increase in total oxidant status (TOS)
and oxidative stress index (OSI) level and a decrease in total antioxidant
status (TAS) level in the 26 and 52 day high voltage groups, compared to the
control group. In the high voltage groups, the nitric oxide (NO) value
increased with the increase of the exposure period. Testicular mass (p>0.05),
TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison. Testicular
weight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 day
comparisons. In routine histopathology data, we detected effects on both the 26
and 52 day HV groups, such as degeneration in spermatic cells and full
structural deterioration and increasing hyalinisation. We also detected an step
up in the ligament cell in the tubular field for the 52-day test groups. In
melatonin added group, proliferating cell nuclear antigen (PCNA)-positive cells
were more active. TUNEL assay analysis showed that there was no significant
difference between the the 26 day experiment groups (p>0.05), but that there
was a significant dissimilarity between the 52 day experiment groups
(p<0.05).



Conclusions:
In our study, the oxidative effect of created by a high voltage results in
changes in serum biochemistry of rats and a number of degenerative
deterioration in the histological structure of testes. Aslo, it is shown that
the oxidative effect created by a high voltage has negative effects on the creation
of spermatogonia. GI partly protects against these effects, while melatonin is
more effective in this regard.

Kaynakça

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Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Yazıları
Yazarlar

Mehmet Cihan Yavaş 0000-0002-2923-050X

Veysi Akpolat Bu kişi benim 0000-0002-2435-7800

Engin Deveci Bu kişi benim 0000-0002-2353-1184

Hakki Murat Bilgin Bu kişi benim 0000-0002-6040-9989

İbrahim Kaplan Bu kişi benim 0000-0003-2813-1064

Ugur Seker 0000-0002-1693-6378

İsmail Yildiz Bu kişi benim 0000-0001-5505-838X

Esref Alkis Bu kişi benim 0000-0002-3321-2873

Mustafa Salih Celik Bu kişi benim 0000-0001-7397-8120

Mehmet Zülküf Akdağ Bu kişi benim 0000-0003-2826-2734

Yayımlanma Tarihi 13 Aralık 2018
Gönderilme Tarihi 16 Aralık 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 45 Sayı: 4

Kaynak Göster

APA Yavaş, M. C., Akpolat, V., Deveci, E., Bilgin, H. M., vd. (2018). Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Tıp Dergisi, 45(4), 447-461. https://doi.org/10.5798/dicletip.497923
AMA Yavaş MC, Akpolat V, Deveci E, Bilgin HM, Kaplan İ, Seker U, Yildiz İ, Alkis E, Celik MS, Akdağ MZ. Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. diclemedj. Aralık 2018;45(4):447-461. doi:10.5798/dicletip.497923
Chicago Yavaş, Mehmet Cihan, Veysi Akpolat, Engin Deveci, Hakki Murat Bilgin, İbrahim Kaplan, Ugur Seker, İsmail Yildiz, Esref Alkis, Mustafa Salih Celik, ve Mehmet Zülküf Akdağ. “Determining the Effect of an Electromagnetic Field Generated by a High Voltage Power Line on Rat Spermatogonia Cells”. Dicle Tıp Dergisi 45, sy. 4 (Aralık 2018): 447-61. https://doi.org/10.5798/dicletip.497923.
EndNote Yavaş MC, Akpolat V, Deveci E, Bilgin HM, Kaplan İ, Seker U, Yildiz İ, Alkis E, Celik MS, Akdağ MZ (01 Aralık 2018) Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Tıp Dergisi 45 4 447–461.
IEEE M. C. Yavaş, “Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells”, diclemedj, c. 45, sy. 4, ss. 447–461, 2018, doi: 10.5798/dicletip.497923.
ISNAD Yavaş, Mehmet Cihan vd. “Determining the Effect of an Electromagnetic Field Generated by a High Voltage Power Line on Rat Spermatogonia Cells”. Dicle Tıp Dergisi 45/4 (Aralık 2018), 447-461. https://doi.org/10.5798/dicletip.497923.
JAMA Yavaş MC, Akpolat V, Deveci E, Bilgin HM, Kaplan İ, Seker U, Yildiz İ, Alkis E, Celik MS, Akdağ MZ. Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. diclemedj. 2018;45:447–461.
MLA Yavaş, Mehmet Cihan vd. “Determining the Effect of an Electromagnetic Field Generated by a High Voltage Power Line on Rat Spermatogonia Cells”. Dicle Tıp Dergisi, c. 45, sy. 4, 2018, ss. 447-61, doi:10.5798/dicletip.497923.
Vancouver Yavaş MC, Akpolat V, Deveci E, Bilgin HM, Kaplan İ, Seker U, Yildiz İ, Alkis E, Celik MS, Akdağ MZ. Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. diclemedj. 2018;45(4):447-61.