Araştırma Makalesi
Yıl 2024,
Cilt: 25 Sayı: 1, 10 - 18, 28.03.2024
Öz
Aims: Some of the most effective medicines discovered to date have been derived from natural products derived from microorganisms, plants and animals. Phenolic compounds have been recognized as the most important bioactive compounds responsible for bone health effects. Ellagic acid (EA), a member of the flavonoids, is a natural phenol antioxidant found in many fruits and vegetables. The anti-proliferative and antioxidant properties of ellagic acid have prompted research into its potential health benefits.1 The aim of our study is to investigate the effect of EA on hard tissue healing after tooth extraction in rats.
Methods: For this purpose, the lower left mandibular teeth of 16 Wistar Albino male rats were extracted under general anesthesia. Among the rats randomly divided into four groups, the 1st study group was given EA by gavage once a day for 14 days (14EA), and the 2nd study group was given EA by gavage once a day for 21 days (21EA). The 1st control group was given 0.9% Isotonic NaCl by gavage once a day for 14 days (14K), the 2nd control group was given 0.9% Isotonic NaCl by gavage once a day for 21 days (21K). Afterwards, the rats were sacrificed and prepared for histopathological examination.
Results: As a result of histopathological examination, the inflammation level of the 21EA group was found to be significantly lower than the 14K (p:0.042) and 21K (p:0.004) groups (p<0.05). The epithelial proliferation level of the 21EA group was found to be significantly higher than the 21K, 14K (p:0.001) and 14EA (p:0.009) groups (p<0.05). The new bone formation level of the 21EA group was higher than the 14K (p:0.001), 21K (p:0.008) and 14EA (p:0.018) groups; The new bone formation level of the 14EA group was statistically significantly higher than the 14K group (p:0.014; p<0.05). The healing score of the 21EA group was found to be significantly higher than the 14EA (p:0.013) (14K (p:0.017) and 21K (p:0.009) groups (p<0.05).
Conclusion: As a result of the study, it was found that EA had a statistically significant positive effect on bone healing, especially on the 21st day.
Anahtar Kelimeler
Kaynakça
- 1. Rojanathammanee L, Puig KL, Combs CK. Pomegranate polyphenols and extract inhibit nuclear factor of activated T-cell activity and microglial activation in vitro and in a transgenic mouse model of Alzheimer disease. J Nutr. 2013;143(5):597-605.
- 2. Nirwana I, Munadziroh E, Yuliati A, et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res. 2022;12(1):116-120.
- 3. Quideau S, Deffieux D, Douat‐Casassus C, Pouységu L. Plant polyphenols: chemical properties, biological activities, and synthesis. Angew Chem Int Ed Engl. 2011;50(3):586-621.
- 4. Al-Obaidi MMJ, Al-Bayaty FH, Al Batran R, Hassandarvish P, Rouhollahi E. Protective effect of ellagic acid on healing alveolar bone after tooth extraction in rat--a histological and immunohistochemical study. Arch Oral Biol. 2014;59(9):987-999.
- 5. Bowden GH. Controlled environment model for accumulation of biofilms of oral bacteria. Methods Enzymol. 1999;310:216-224. 6. Ferrazzano GF, Amato I, Ingenito A, Zarrelli A, Pinto G, Pollio A. Plant polyphenols and their anti-cariogenic properties: a review. Molecules. 2011;16(2):1486-1507.
- 7. Sharifi-Rad J, Quispe C, Castillo CMS, et al. Ellagic acid: a review on its natural sources, chemical stability, and therapeutic potential. Oxid Med Cell Longev. 2022;2022:3848084.
- 8. Ito S, Kasahara N, Kitamura K, et al. Pathological differences in the bone healing processes between tooth extraction socket and femoral fracture. Bone Rep. 2022;16:101522.
- 9. Caneva M, Botticelli D, Stellini E, Souza SLS, Salata LA, Lang NP. Magnesium-enriched hydroxyapatite at immediate implants: a histomorphometric study in dogs. Clin Oral Implants Res. 2011;22(5):512-517.
- 10. Iovine C, Mottola F, Santonastaso M, Finelli R, Agarwal A, Rocco L. In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa. Mol Reprod Dev. 2021;88(2):167-174.
- 11. Aishwarya V, Solaipriya S, Sivaramakrishnan V. Role of ellagic acid for the prevention and treatment of liver diseases. Phytother Res. 2021;35(6):2925-2944.
- 12. Bala I, Bhardwaj V, Hariharan S, Kumar MR. Analytical methods for assay of ellagic acid and its solubility studies. J Pharm Biomed Anal. 2006;40(1):206-210.
- 13. Deepika, Maurya PK. Ellagic acid: insight into its protective effects in age-associated disorders. 3 Biotech. 2022;12(12):340.
- 14. Ahmed S, Rahman A, Saleem M, Athar M, Sultana S. Ellagic acid ameliorates nickel induced biochemical alterations: diminution of oxidative stress. Hum Exp Toxicol. 1999;18(11):691-698.
- 15. Mishra S, Vinayak M. Ellagic acid inhibits PKC signaling by improving antioxidant defense system in urine T cell lymphoma. Mol Biol Rep. 2014;41(7):4187-4197.
- 16. McKee MD, Pedraza CE, Kaartinen MT. Osteopontin and wound healing in bone. Cells Tissues Organs. 2011;194(2-4):313-319.
- 17. Lin X, Yuan G, Li Z, et al. Ellagic acid protects ovariectomy-induced bone loss in mice by inhibiting osteoclast differentiation and bone resorption. J Cell Physiol. 2020;235(9):5951-5961.
- 18. Öngöz Dede F, Bozkurt Doğan Ş, Balli U, et al. The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats. J Appl Oral Sci. 2021;29:e20210160.
- 19. Nirwana I, Rachmadi P, Rianti D. Potential of pomegranate fruit extract (Punica granatum Linn.) to increase vascular endothelial growth factor and platelet-derived growth factor expressions on the post-tooth extraction wound of Cavia cobaya. Vet World. 2017;10(8):999-1003.
- 20. Nirwana I, Munadziroh E, Yuliati A, et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res. 2022;12(1):116-120.
- 21. Primasari DN, Nirwana I, Budi HS, et al., The cytokine and bone protein expression by ellagic acid-hydroxyapatite in bone remodelling model. Scientif World J. 2022;2022:6740853.
- 22. Gül M, Dundar S, Yigin A, et al. Evaluation of the effects of topical ellagic acid and graft application on bone regeneration: an experimental study. J Oral Maxillofac Res. 2022;13(2):e3.
- 23. Al-Obaidi MMJ, Al-Bayaty FH, Al Batran R, Hussaini J, Khor GH. Impact of ellagic acid in bone formation after tooth extraction: an experimental study on diabetic rats. Scientif World J. 2014;2014:908098.
- 24. Wardhana AS, Nirwana I, Budi HS, Surboyo MDC. Role of hydroxyapatite and ellagic acid in the osteogenesis. Eur J Dent. 2021;15(1):8-12.
- 25. Ankaralı H, Ankaralı S. Hayvan deneylerinde verimliliği artıracak deney tasarımları ve denek sayısı. Anat Clin J Med Sci. 2019;24(3):248-258.
Yıl 2024,
Cilt: 25 Sayı: 1, 10 - 18, 28.03.2024
Öz
Kaynakça
- 1. Rojanathammanee L, Puig KL, Combs CK. Pomegranate polyphenols and extract inhibit nuclear factor of activated T-cell activity and microglial activation in vitro and in a transgenic mouse model of Alzheimer disease. J Nutr. 2013;143(5):597-605.
- 2. Nirwana I, Munadziroh E, Yuliati A, et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res. 2022;12(1):116-120.
- 3. Quideau S, Deffieux D, Douat‐Casassus C, Pouységu L. Plant polyphenols: chemical properties, biological activities, and synthesis. Angew Chem Int Ed Engl. 2011;50(3):586-621.
- 4. Al-Obaidi MMJ, Al-Bayaty FH, Al Batran R, Hassandarvish P, Rouhollahi E. Protective effect of ellagic acid on healing alveolar bone after tooth extraction in rat--a histological and immunohistochemical study. Arch Oral Biol. 2014;59(9):987-999.
- 5. Bowden GH. Controlled environment model for accumulation of biofilms of oral bacteria. Methods Enzymol. 1999;310:216-224. 6. Ferrazzano GF, Amato I, Ingenito A, Zarrelli A, Pinto G, Pollio A. Plant polyphenols and their anti-cariogenic properties: a review. Molecules. 2011;16(2):1486-1507.
- 7. Sharifi-Rad J, Quispe C, Castillo CMS, et al. Ellagic acid: a review on its natural sources, chemical stability, and therapeutic potential. Oxid Med Cell Longev. 2022;2022:3848084.
- 8. Ito S, Kasahara N, Kitamura K, et al. Pathological differences in the bone healing processes between tooth extraction socket and femoral fracture. Bone Rep. 2022;16:101522.
- 9. Caneva M, Botticelli D, Stellini E, Souza SLS, Salata LA, Lang NP. Magnesium-enriched hydroxyapatite at immediate implants: a histomorphometric study in dogs. Clin Oral Implants Res. 2011;22(5):512-517.
- 10. Iovine C, Mottola F, Santonastaso M, Finelli R, Agarwal A, Rocco L. In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa. Mol Reprod Dev. 2021;88(2):167-174.
- 11. Aishwarya V, Solaipriya S, Sivaramakrishnan V. Role of ellagic acid for the prevention and treatment of liver diseases. Phytother Res. 2021;35(6):2925-2944.
- 12. Bala I, Bhardwaj V, Hariharan S, Kumar MR. Analytical methods for assay of ellagic acid and its solubility studies. J Pharm Biomed Anal. 2006;40(1):206-210.
- 13. Deepika, Maurya PK. Ellagic acid: insight into its protective effects in age-associated disorders. 3 Biotech. 2022;12(12):340.
- 14. Ahmed S, Rahman A, Saleem M, Athar M, Sultana S. Ellagic acid ameliorates nickel induced biochemical alterations: diminution of oxidative stress. Hum Exp Toxicol. 1999;18(11):691-698.
- 15. Mishra S, Vinayak M. Ellagic acid inhibits PKC signaling by improving antioxidant defense system in urine T cell lymphoma. Mol Biol Rep. 2014;41(7):4187-4197.
- 16. McKee MD, Pedraza CE, Kaartinen MT. Osteopontin and wound healing in bone. Cells Tissues Organs. 2011;194(2-4):313-319.
- 17. Lin X, Yuan G, Li Z, et al. Ellagic acid protects ovariectomy-induced bone loss in mice by inhibiting osteoclast differentiation and bone resorption. J Cell Physiol. 2020;235(9):5951-5961.
- 18. Öngöz Dede F, Bozkurt Doğan Ş, Balli U, et al. The effect of ellagic acid on the repair process of periodontal defects related to experimental periodontitis in rats. J Appl Oral Sci. 2021;29:e20210160.
- 19. Nirwana I, Rachmadi P, Rianti D. Potential of pomegranate fruit extract (Punica granatum Linn.) to increase vascular endothelial growth factor and platelet-derived growth factor expressions on the post-tooth extraction wound of Cavia cobaya. Vet World. 2017;10(8):999-1003.
- 20. Nirwana I, Munadziroh E, Yuliati A, et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res. 2022;12(1):116-120.
- 21. Primasari DN, Nirwana I, Budi HS, et al., The cytokine and bone protein expression by ellagic acid-hydroxyapatite in bone remodelling model. Scientif World J. 2022;2022:6740853.
- 22. Gül M, Dundar S, Yigin A, et al. Evaluation of the effects of topical ellagic acid and graft application on bone regeneration: an experimental study. J Oral Maxillofac Res. 2022;13(2):e3.
- 23. Al-Obaidi MMJ, Al-Bayaty FH, Al Batran R, Hussaini J, Khor GH. Impact of ellagic acid in bone formation after tooth extraction: an experimental study on diabetic rats. Scientif World J. 2014;2014:908098.
- 24. Wardhana AS, Nirwana I, Budi HS, Surboyo MDC. Role of hydroxyapatite and ellagic acid in the osteogenesis. Eur J Dent. 2021;15(1):8-12.
- 25. Ankaralı H, Ankaralı S. Hayvan deneylerinde verimliliği artıracak deney tasarımları ve denek sayısı. Anat Clin J Med Sci. 2019;24(3):248-258.
Toplam 24 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Ağız ve Çene Cerrahisi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 28 Mart 2024 |
| Gönderilme Tarihi | 8 Şubat 2024 |
| Kabul Tarihi | 26 Mart 2024 |
| Yayımlandığı Sayı | Yıl 2024 Cilt: 25 Sayı: 1 |
