Öz
Objective: Neuropathic pain and treatment side effects decreasing quality of life, reducing productivity, and high costs due to long duration of treatment. Regenerative medicine is a new and effective alternative treatment for neuropathic pain, one of which is Platelet-Rich Plasma (PRP) therapy. The objective if this study is to prove that there are differences in neuroregeneration post-crush injury after FD-PRP administration. Materials and Methods: Neuropathic pain model was made with crushing method, by compressing the infraorbital nerve using the artery clamp for 15 seconds. Rats divided into six groups. Each group was observed on day 14 (A, B, C) and 21 (A', B', C') to observe the macrophages, lymphocytes, and Schwann cells with Hematoxylin Eosin staining seen on horizontal plane of the infraorbital nerve with 400x magnification. Face grooming observations were performed on day 0 (A0, B0, C0), day 7 (A7, B7, C7), day 14 (A14, B14, C14), day 21 (A21, B21, C21). Results: There were significant differences in face grooming frequencies between groups on day 0 (p = 0.002). ANOVA Same Subject test on A`, B`, and C` revealed significant differences in macrophages and lymphocytes with score 0.02 (p <0.05) and 0.013 (p<0.05), respectively. There were significant differences between group A' and B' and between B' and C' (p>0.05). Conclusions: FD-PRP promotes nerve regeneration in axonotmesis, which was characterized by a decrease in face grooming frequency on day 7 and an increase in the number of lymphocytes, macrophages and Schwann cells on day 21.
Anahtar Kelimeler
Platelet-Rich Plasma Macrophages Lymphocytes Schwann Cells Peripheral Nerve Injuries
Destekleyen Kurum
Faculty of Dental Medicine Universitas Airlangga - Surabaya Indonesia
Proje Numarası
grant numbers 1408/UN3/2019
Teşekkür
The authors would thank to the Stem Cell Center and Tissue Bank of Dr. Soetomo Hospital, Surabaya for its support.
Kaynakça
- 1. Jensen, T.S., Baron, R., Haanpää, M., Kalso, E., Loeser, J.D., Rice, A.S.C. and Treede, R.D. (2011), “A new definition of neuropathic pain”, Pain, International Association for the Study of Pain, Vol. 152 No. 10, pp. 2204–2205. 2. Obata, H. (2017). Analgesic Mechanisms of Antidepressants for Neuropathic Pain. International Journal of Molecular Sciences, 18(2483), pp. 1-12. doi: 10.3390/ijms18112483. 3. Xie, W., Strong, J. and Zhang, J. (2017). Active Nerve Regeneration with Failed Target Reinnervation Drives Persistent Neuropathic Pain. Eneuro, 4(1), pp. 1–16. doi: 10.1523/eneuro.0008-17.2017. 4. Oudelaar, B., Peerbooms, J., Huis in ‘t Veld, R. and Vochteloo, A. (2018). Concentrations of Blood Components in Commercial Platelet-Rich Plasma Separation Systems: A Review of the Literature. The American Journal of Sports Medicine, 47(2), pp. 1–8. doi: 10.1177/0363546517746112. 5. Kawase, T. (2015). Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances. Odontology, Springer Japan, 103(2), pp. 126–135. doi: 10.1007/s10266-015-0209-2. 6. Menorca, R.M.G., Fussel, T.S., Elfar, J.C., 2015. Peripheral Nerve Trauma : Mechanisms of Injury and Recovery. Hand Clin. Author Manuscr. 29, 317–330. 7. Romero-reyes, M., Akerman, S., Nguyen, E., Vijjeswarapu, A., Hom, B., Dong, H., Charles, A.C., 2012. Spontaneous Behavioral Responses in the Orofacial Region : A Model of Trigeminal Pain in Mouse. Am. Headache Soc. Res. 5010, 137–151. 8. Deseure, K., Hans, G.H., 2015. Chronic Constriction Injury of the Rat’s Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain. J. Vis. Exp. 1–6. 9. Kalueff A V, Stewart A M, Song C, Berridge K C, Graybiel A M, Fentress J C. 2016. Neurobiology of Rodent Self-Grooming and Its Value for Translational Neuroscience. Nat Rev Neurosci. 17(1): 45–59. 10. Xu, M., Aita, M., Charles, C., 2008. Partial infraorbital nerve ligation as a model of trigeminal nerve injury in the mouse: Behavioral, Neural and Glial reactions. J. Pain 9, 1036–1048. 11. Alvites, R, Caseiro A R, Pedrosa S S, Branquinho M V, Ronchi G, Geuna S, Varejao A S P, Mauricio A C. 2018. Peripheral Nerve Injury and Axonotmesis: State of The Art and Recent Advances. Cogent Medicine. p.8 12. Souza, I., Vasconcelos, A G C., Caumo, W., Baptista, A F. 2017. Resilience Profile of Patients with Chronic Pain. Cad. Saúde Pública, 33(1):e00146915. 13. Yeung, E W., Zautra, A., Arewasikporn, A. 2012. Resilience and Chronic Pain. Journal of Social and Clinical Psychology, Vol. 31, No. 6, pp. 593-617. 14. W. Daly1, L. Yao1, D. Zeugolis1, A. Windebank2 and A. Pandit. A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery. 2011.J. R. Soc. Interface. 2:452 15. Geuna, S., Raimondo, S., Ronchi, G., Di Scipio, F., Tos, P., Czaja, K. and Fornaro, M. (2009), Chapter 3 Histology of the Peripheral Nerve and Changes Occurring During Nerve Regeneration, International Review of Neurobiology, 1st ed., Vol. 87, Elsevier Inc., available at:https://doi.org/10.1016/S0074-7742(09)87003-7. 16. Mahadewa, Tjokorda Gde Bgus. 2013. Saraf Perifer Masalah dan Penanganannya. Permata Puri Media: Jakarta Barat. 17. Kumar, V., Abbas, abul K. and Aster, J.C. (2013), Basic Pathology, Journal of Clinical Pathology, Vol. 47, available at:https://doi.org/10.1136/jcp.47.1.95-d. 18. Kuffler, Damien P. 2013. Platelet-Rich Plasma and the Elimination of Neuropathic Pain. Molecular Neurobiology. p. 1-18 19. Sánchez, Mikel., Garate, Ane., Delgado, Diego., Padilla, Sabino. 2017. Platelet-Rich Plasma, an Adjuvant Biological Therapy to Assist Peripheral Nerve Repair. Neural Regeneration Research Journal. Vol.12(1). p.47–52. 20. Czaja, K., Burns, G. A., and Ritter, R. C. 2008. Capsaicin-Induced Neuronal Death and Proliferation of The Primary Sensory Neurons Located in The Nodose Ganglia of Adult Rats. Neuroscience. Vol.154. p.621–630. 21. Su, H.X., Cho, E.Y. 2003. Sprouting of Axon-like Processes From Axotomized Retinal Ganglion Cells Induced by Normal and Preinjured Intravitreal Optic Nerve Grafts. Brain Research. Vol.991. p.150–162. 22. Painter, M.W., Brosius, Lutz A., Cheng, Y.C., Latremoliere, A., Duong, K., Miller, C.M., Posada, S., Cobos, E.J., Zhang, A.X., Wagers, A.J., Havton, L.A., Barres, B., Omura, T., Woolf, C.J. 2014. Diminished Schwann Cell Repair Responses Underlie Age-Associated Impaired Axonal Regeneration. Neuron. Vol. 83. p.331–343. 23. Scheib J, Hoke A. 2013. Advances in Peripheral Nerve Regeneration. Nature Reviews Neurology. Vol.9. p.668–676. 24. Zochodne DW. 2012. The Challenges and Beauty of Peripheral Nerve Regrowth. Journal of Peripheral Nervous System. Vol. 17. p.1–187.
Öz
Proje Numarası
grant numbers 1408/UN3/2019
Kaynakça
- 1. Jensen, T.S., Baron, R., Haanpää, M., Kalso, E., Loeser, J.D., Rice, A.S.C. and Treede, R.D. (2011), “A new definition of neuropathic pain”, Pain, International Association for the Study of Pain, Vol. 152 No. 10, pp. 2204–2205. 2. Obata, H. (2017). Analgesic Mechanisms of Antidepressants for Neuropathic Pain. International Journal of Molecular Sciences, 18(2483), pp. 1-12. doi: 10.3390/ijms18112483. 3. Xie, W., Strong, J. and Zhang, J. (2017). Active Nerve Regeneration with Failed Target Reinnervation Drives Persistent Neuropathic Pain. Eneuro, 4(1), pp. 1–16. doi: 10.1523/eneuro.0008-17.2017. 4. Oudelaar, B., Peerbooms, J., Huis in ‘t Veld, R. and Vochteloo, A. (2018). Concentrations of Blood Components in Commercial Platelet-Rich Plasma Separation Systems: A Review of the Literature. The American Journal of Sports Medicine, 47(2), pp. 1–8. doi: 10.1177/0363546517746112. 5. Kawase, T. (2015). Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances. Odontology, Springer Japan, 103(2), pp. 126–135. doi: 10.1007/s10266-015-0209-2. 6. Menorca, R.M.G., Fussel, T.S., Elfar, J.C., 2015. Peripheral Nerve Trauma : Mechanisms of Injury and Recovery. Hand Clin. Author Manuscr. 29, 317–330. 7. Romero-reyes, M., Akerman, S., Nguyen, E., Vijjeswarapu, A., Hom, B., Dong, H., Charles, A.C., 2012. Spontaneous Behavioral Responses in the Orofacial Region : A Model of Trigeminal Pain in Mouse. Am. Headache Soc. Res. 5010, 137–151. 8. Deseure, K., Hans, G.H., 2015. Chronic Constriction Injury of the Rat’s Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain. J. Vis. Exp. 1–6. 9. Kalueff A V, Stewart A M, Song C, Berridge K C, Graybiel A M, Fentress J C. 2016. Neurobiology of Rodent Self-Grooming and Its Value for Translational Neuroscience. Nat Rev Neurosci. 17(1): 45–59. 10. Xu, M., Aita, M., Charles, C., 2008. Partial infraorbital nerve ligation as a model of trigeminal nerve injury in the mouse: Behavioral, Neural and Glial reactions. J. Pain 9, 1036–1048. 11. Alvites, R, Caseiro A R, Pedrosa S S, Branquinho M V, Ronchi G, Geuna S, Varejao A S P, Mauricio A C. 2018. Peripheral Nerve Injury and Axonotmesis: State of The Art and Recent Advances. Cogent Medicine. p.8 12. Souza, I., Vasconcelos, A G C., Caumo, W., Baptista, A F. 2017. Resilience Profile of Patients with Chronic Pain. Cad. Saúde Pública, 33(1):e00146915. 13. Yeung, E W., Zautra, A., Arewasikporn, A. 2012. Resilience and Chronic Pain. Journal of Social and Clinical Psychology, Vol. 31, No. 6, pp. 593-617. 14. W. Daly1, L. Yao1, D. Zeugolis1, A. Windebank2 and A. Pandit. A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery. 2011.J. R. Soc. Interface. 2:452 15. Geuna, S., Raimondo, S., Ronchi, G., Di Scipio, F., Tos, P., Czaja, K. and Fornaro, M. (2009), Chapter 3 Histology of the Peripheral Nerve and Changes Occurring During Nerve Regeneration, International Review of Neurobiology, 1st ed., Vol. 87, Elsevier Inc., available at:https://doi.org/10.1016/S0074-7742(09)87003-7. 16. Mahadewa, Tjokorda Gde Bgus. 2013. Saraf Perifer Masalah dan Penanganannya. Permata Puri Media: Jakarta Barat. 17. Kumar, V., Abbas, abul K. and Aster, J.C. (2013), Basic Pathology, Journal of Clinical Pathology, Vol. 47, available at:https://doi.org/10.1136/jcp.47.1.95-d. 18. Kuffler, Damien P. 2013. Platelet-Rich Plasma and the Elimination of Neuropathic Pain. Molecular Neurobiology. p. 1-18 19. Sánchez, Mikel., Garate, Ane., Delgado, Diego., Padilla, Sabino. 2017. Platelet-Rich Plasma, an Adjuvant Biological Therapy to Assist Peripheral Nerve Repair. Neural Regeneration Research Journal. Vol.12(1). p.47–52. 20. Czaja, K., Burns, G. A., and Ritter, R. C. 2008. Capsaicin-Induced Neuronal Death and Proliferation of The Primary Sensory Neurons Located in The Nodose Ganglia of Adult Rats. Neuroscience. Vol.154. p.621–630. 21. Su, H.X., Cho, E.Y. 2003. Sprouting of Axon-like Processes From Axotomized Retinal Ganglion Cells Induced by Normal and Preinjured Intravitreal Optic Nerve Grafts. Brain Research. Vol.991. p.150–162. 22. Painter, M.W., Brosius, Lutz A., Cheng, Y.C., Latremoliere, A., Duong, K., Miller, C.M., Posada, S., Cobos, E.J., Zhang, A.X., Wagers, A.J., Havton, L.A., Barres, B., Omura, T., Woolf, C.J. 2014. Diminished Schwann Cell Repair Responses Underlie Age-Associated Impaired Axonal Regeneration. Neuron. Vol. 83. p.331–343. 23. Scheib J, Hoke A. 2013. Advances in Peripheral Nerve Regeneration. Nature Reviews Neurology. Vol.9. p.668–676. 24. Zochodne DW. 2012. The Challenges and Beauty of Peripheral Nerve Regrowth. Journal of Peripheral Nervous System. Vol. 17. p.1–187.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Original Research Articles |
| Yazarlar | |
| Proje Numarası | grant numbers 1408/UN3/2019 |
| Yayımlanma Tarihi | 31 Aralık 2020 |
| Gönderilme Tarihi | 20 Nisan 2020 |
| Yayımlandığı Sayı | Yıl 2020 |
Cited By
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