Lack of Association between Enamel Gene Variants and Dental Caries in Adults

Gül Yıldız Telatar; Faruk Saydam

doi:10.7126/cumudj.690129

Research Article

Lack of Association between Enamel Gene Variants and Dental Caries in Adults

Year 2020, , 96 - 106, 30.06.2020

Gül Yıldız Telatar , Faruk Saydam

https://doi.org/10.7126/cumudj.690129

Cited By: 2

Abstract

Objectives: Studies report that gene polymorphisms associated with mineralization
may change the structure of enamel and create a predisposition for developing
dental caries. The aim of the study was to evaluate the VDR and TFIP11 gene
variants in adults with caries experience and to investigate their interactions
with the environmental factors. Materials
and Methods: A total of 160 individuals at the age of 24-40 years were
included in the study and they were assigned to two groups according to
decayed-missing-filled teeth index (DMFT); namely the low caries experience
(LCE, DMFT≤4) and high caries experience (HCE, DMFT> 9.13). DNA was isolated from buccal swab samples to
genotype the VDR (TaqI; rs731236) and
TFIP11 (rs5997096) gene variants. The
real-time PCR was used for genotyping. The frequency of tooth brushing, carbohydrate
intake, smoking, and the dental plaques were evaluated as environmental risk
factors. Results: Between the caries
groups and the distribution of the genotypes and alleles of the VDR rs731236 and TFIP11 rs5997096 gene variants were not statistically different.
There was also no significant difference when homozygous, heterozygous,
dominant, and recessive models were evaluated for the two variants. The
frequency of tooth brushing was significantly higher in the LCE group.
According to the regression analysis; the amount of plaque explained the high
caries experience at a rate of 51.4%. Conclusions:
The study findings indicated that common
variants in the VDR and TFIP11 genes were not associated with high caries
experiences in Turkish adults.

Keywords

Dental caries, enamel, genes, vitamin D receptor

Supporting Institution

Recep Tayyip Erdogan University Scientific Research Projects Foundation

Project Number

TSA-2017-772

References

References 1 . Selwitz RH, Ismail AI, Pitts NB. Dental caries definition. Lancet 2007; 369: 51–59.
2. Al Mulla AH, Al Kharsa S, Kjellberg H, Birkhed D. Caries risk profiles in orthodontic patients at follow-up using cariogram. Angle Orthod 2008; 79: 323–330.
3. Miravet AR, Montiel Company JM, Almerich Silla JM. Evaluation of caries risk in a young adult population. Med Oral Patol Oral Cir Bucal 2007; 12.
4. Werneck RI, Mira MT, Trevilatto PC. A critical review: An overview of genetic influence on dental caries. Oral Dis 2010; 16: 613–623.
5. Patir A, Seymen F, Yildirim M et al. Enamel formation genes are associated with high caries experience in Turkish children. Caries Res 2008; 42: 394–400.
6. Slayton RL, Cooper ME, Marazita ML. Tuftelin, mutans streptococci, and dental caries susceptibility. J Dent Res 2005; 84: 711–714.
7. Gasse B, Grabar S, Lafont AG et al. Common SNPs of amelogeninX (AMELX) and dental caries susceptibility. J Dent Res 2013; 92: 418–424.
8. Ouryouji K, Imamura Y, Fujigaki Y et al. Analysis of mutations in the amelogenin and the enamelin genes in severe caries in Japanese pediatric patients. Pediatr Dent J 2008; 18: 79–85.
9. Vieira AR, Modesto A, Marazita ML. Caries: Review of human genetics research. Caries Res 2014; 48: 491–506.
10. Renuka P. Review On “Influence Of Host Genes On Dental Caries”. IOSR J Dent Med Sci 2013; 4: 86–92.
11. Hu XP, Li ZQ, Zhou JY, Yu ZH, Zhang JM, Guo ML. Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population. Genet Mol Res 2015; 14: 11631–11638.
12. Kong Y yuan, Zheng J mao, Zhang W juan et al. The relationship between vitamin D receptor gene polymorphism and deciduous tooth decay in Chinese children. BMC Oral Health 2017; 17: 1–6.
13. Izakovicova Holla L, Borilova Linhartova P, Kastovsky J et al. Vitamin D Receptor Taq i Gene Polymorphism and Dental Caries in Czech Children. Caries Res 2017; 51: 7–11.
14. Cogulu D, Onay H, Ozdemir Y, Aslan GI, Ozkinay F, Eronat C. The role of Vitamin D receptor polymorphisms on dental caries. J Clin Pediatr Dent 2016; 40: 211–214.
15. Yu M, Jiang QZ, Sun ZY, Kong YY, Chen Z. Association between single nucleotide polymorphisms in Vitamin D receptor gene polymorphisms and permanent tooth caries susceptibility to permanent tooth caries in Chinese adolescent. Biomed Res Int 2017; 2017. doi:10.1155/2017/4096316.
16. Shimizu T, Ho B, Deeley K et al. Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge. PLoS One 2012; 7. doi:10.1371/journal.pone.0045022.
17. Yildiz G, Ermis RB, Calapoglu NS, Celik EU, Türel GY. Gene-environment Interactions in the Etiology of Dental Caries. J Dent Res 2016; 95: 74–79.
18. Wang X, Shaffer JR, Zeng Z et al. Genome-wide association Scan of dental caries in the permanent dentition. BMC Oral Health 2012; 12. doi:10.1186/1472-6831-12-57.
19. Deeley K, Letra A, Rose EK et al. Possible association of amelogenin to high caries experience in a guatemalan-mayan population. Caries Res 2008; 42: 8–13.
20. Pang L, Zhi Q, Zhuang P, Yu L, Tao Y, Lin H. Variation in enamel formation genes influences enamel demineralization in vitro in a Streptococcus mutans biofilm model. Front Physiol 2017; 8: 1–11.
21.Jeremias F, Koruyucu M, Küchler EC et al. Genes expressed in dental enamel development are associated with molar-incisor hypomineralization. Arch Oral Biol 2013; 58: 1434–1442.
22. Shaffer JR, Carlson JC, Stanley BOC et al. Effects of enamel matrix genes on dental caries are moderated by fluoride exposures. 2015; 134: 159–167.
23. WHO. WHO Guidelines: Oral Health Surveys - Basic Methods. 1997.
24. Silness J LH. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol 1964; 22: 121–135.
25. Rintakoski K, Kaprio J, Murtomaa H. Genetic and environmental factors in oral health among twins. J Dent Res 2010; 89: 700–704.
26. Reich E, Lussi A, Newbrun E. Caries-risk assessment. Int Dent J 1999; 49: 15–26.
27. Fontana M, Zero DT. Assessing patients’ caries risk. J Am Dent Assoc 2006; 137: 1231–1239.
28. Kang S, Yoon I, Lee H, Cho J. Association between AMELX polymorphisms and dental caries in Koreans. Oral Dis 2011; 17: 399–406.
29. Olszowski T, Adler G, Janiszewska-Olszowska J, Safranow K, Kaczmarczyk M. MBL2, MASP2, AMELX, and ENAM gene polymorphisms and dental caries in Polish children. Oral Dis 2012; 18: 389–395.
30. Tannure PN, Küchler EC, Lips A et al. Genetic variation in MMP20 contributes to higher caries experience. J Dent 2012; 40: 381–386.
31. Petersen PE. The World Oral Health Report 2003: continuous improvement of oral health in the 21st century--the approach of the WHO Global Oral Health Programme. Community Dent Oral Epidemiol 2003; 31: 3–23.
32. Zhang X, Rahemtulla F, Zhang P, Beck P TH. Different enamel and dentin mineralization observed in VDR deficient mouse model. Arch Oral Biol 2009; 54: 299–305.
33. Abbasoglu Z, Tanboga İ, Küchler EC, Deeley K, Weber M, Kaspar C, Korachi M VA. Early Childhood Caries is Associated with Genetic Variants in Enamel Formation and Immune Response Genes. Caries Res 2015; 49: 70–77.
34. Gerreth K, Zaorska K, Zabel M, Borysewicz-Lewicka M, Nowicki M. Chosen single nucleotide polymorphisms (SNPs) of enamel formation genes and dental caries in a population of Polish children. Adv Clin Exp Med 2017; 26: 899–905.
35. Weber M, Bogstad Søvik J, Mulic A et al. Redefining the Phenotype of Dental Caries. Caries Res 2018; 52: 263–271.
36. Williams SD, Hughes TE, Adler CJ, Brook AH, Townsend GC. Epigenetics: A new frontier in dentistry. Aust Dent J 2014; 59: 23–33.
37. Fernando S, Speicher DJ, Bakr MM et al. Protocol for assessing maternal, environmental and epigenetic risk factors for dental caries in children. BMC Oral Health 2015; 15: 1–8.

Year 2020, , 96 - 106, 30.06.2020

Gül Yıldız Telatar , Faruk Saydam

https://doi.org/10.7126/cumudj.690129

Cited By: 2

Abstract

Project Number

TSA-2017-772

References

References 1 . Selwitz RH, Ismail AI, Pitts NB. Dental caries definition. Lancet 2007; 369: 51–59.
2. Al Mulla AH, Al Kharsa S, Kjellberg H, Birkhed D. Caries risk profiles in orthodontic patients at follow-up using cariogram. Angle Orthod 2008; 79: 323–330.
3. Miravet AR, Montiel Company JM, Almerich Silla JM. Evaluation of caries risk in a young adult population. Med Oral Patol Oral Cir Bucal 2007; 12.
4. Werneck RI, Mira MT, Trevilatto PC. A critical review: An overview of genetic influence on dental caries. Oral Dis 2010; 16: 613–623.
5. Patir A, Seymen F, Yildirim M et al. Enamel formation genes are associated with high caries experience in Turkish children. Caries Res 2008; 42: 394–400.
6. Slayton RL, Cooper ME, Marazita ML. Tuftelin, mutans streptococci, and dental caries susceptibility. J Dent Res 2005; 84: 711–714.
7. Gasse B, Grabar S, Lafont AG et al. Common SNPs of amelogeninX (AMELX) and dental caries susceptibility. J Dent Res 2013; 92: 418–424.
8. Ouryouji K, Imamura Y, Fujigaki Y et al. Analysis of mutations in the amelogenin and the enamelin genes in severe caries in Japanese pediatric patients. Pediatr Dent J 2008; 18: 79–85.
9. Vieira AR, Modesto A, Marazita ML. Caries: Review of human genetics research. Caries Res 2014; 48: 491–506.
10. Renuka P. Review On “Influence Of Host Genes On Dental Caries”. IOSR J Dent Med Sci 2013; 4: 86–92.
11. Hu XP, Li ZQ, Zhou JY, Yu ZH, Zhang JM, Guo ML. Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population. Genet Mol Res 2015; 14: 11631–11638.
12. Kong Y yuan, Zheng J mao, Zhang W juan et al. The relationship between vitamin D receptor gene polymorphism and deciduous tooth decay in Chinese children. BMC Oral Health 2017; 17: 1–6.
13. Izakovicova Holla L, Borilova Linhartova P, Kastovsky J et al. Vitamin D Receptor Taq i Gene Polymorphism and Dental Caries in Czech Children. Caries Res 2017; 51: 7–11.
14. Cogulu D, Onay H, Ozdemir Y, Aslan GI, Ozkinay F, Eronat C. The role of Vitamin D receptor polymorphisms on dental caries. J Clin Pediatr Dent 2016; 40: 211–214.
15. Yu M, Jiang QZ, Sun ZY, Kong YY, Chen Z. Association between single nucleotide polymorphisms in Vitamin D receptor gene polymorphisms and permanent tooth caries susceptibility to permanent tooth caries in Chinese adolescent. Biomed Res Int 2017; 2017. doi:10.1155/2017/4096316.
16. Shimizu T, Ho B, Deeley K et al. Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge. PLoS One 2012; 7. doi:10.1371/journal.pone.0045022.
17. Yildiz G, Ermis RB, Calapoglu NS, Celik EU, Türel GY. Gene-environment Interactions in the Etiology of Dental Caries. J Dent Res 2016; 95: 74–79.
18. Wang X, Shaffer JR, Zeng Z et al. Genome-wide association Scan of dental caries in the permanent dentition. BMC Oral Health 2012; 12. doi:10.1186/1472-6831-12-57.
19. Deeley K, Letra A, Rose EK et al. Possible association of amelogenin to high caries experience in a guatemalan-mayan population. Caries Res 2008; 42: 8–13.
20. Pang L, Zhi Q, Zhuang P, Yu L, Tao Y, Lin H. Variation in enamel formation genes influences enamel demineralization in vitro in a Streptococcus mutans biofilm model. Front Physiol 2017; 8: 1–11.
21.Jeremias F, Koruyucu M, Küchler EC et al. Genes expressed in dental enamel development are associated with molar-incisor hypomineralization. Arch Oral Biol 2013; 58: 1434–1442.
22. Shaffer JR, Carlson JC, Stanley BOC et al. Effects of enamel matrix genes on dental caries are moderated by fluoride exposures. 2015; 134: 159–167.
23. WHO. WHO Guidelines: Oral Health Surveys - Basic Methods. 1997.
24. Silness J LH. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol 1964; 22: 121–135.
25. Rintakoski K, Kaprio J, Murtomaa H. Genetic and environmental factors in oral health among twins. J Dent Res 2010; 89: 700–704.
26. Reich E, Lussi A, Newbrun E. Caries-risk assessment. Int Dent J 1999; 49: 15–26.
27. Fontana M, Zero DT. Assessing patients’ caries risk. J Am Dent Assoc 2006; 137: 1231–1239.
28. Kang S, Yoon I, Lee H, Cho J. Association between AMELX polymorphisms and dental caries in Koreans. Oral Dis 2011; 17: 399–406.
29. Olszowski T, Adler G, Janiszewska-Olszowska J, Safranow K, Kaczmarczyk M. MBL2, MASP2, AMELX, and ENAM gene polymorphisms and dental caries in Polish children. Oral Dis 2012; 18: 389–395.
30. Tannure PN, Küchler EC, Lips A et al. Genetic variation in MMP20 contributes to higher caries experience. J Dent 2012; 40: 381–386.
31. Petersen PE. The World Oral Health Report 2003: continuous improvement of oral health in the 21st century--the approach of the WHO Global Oral Health Programme. Community Dent Oral Epidemiol 2003; 31: 3–23.
32. Zhang X, Rahemtulla F, Zhang P, Beck P TH. Different enamel and dentin mineralization observed in VDR deficient mouse model. Arch Oral Biol 2009; 54: 299–305.
33. Abbasoglu Z, Tanboga İ, Küchler EC, Deeley K, Weber M, Kaspar C, Korachi M VA. Early Childhood Caries is Associated with Genetic Variants in Enamel Formation and Immune Response Genes. Caries Res 2015; 49: 70–77.
34. Gerreth K, Zaorska K, Zabel M, Borysewicz-Lewicka M, Nowicki M. Chosen single nucleotide polymorphisms (SNPs) of enamel formation genes and dental caries in a population of Polish children. Adv Clin Exp Med 2017; 26: 899–905.
35. Weber M, Bogstad Søvik J, Mulic A et al. Redefining the Phenotype of Dental Caries. Caries Res 2018; 52: 263–271.
36. Williams SD, Hughes TE, Adler CJ, Brook AH, Townsend GC. Epigenetics: A new frontier in dentistry. Aust Dent J 2014; 59: 23–33.
37. Fernando S, Speicher DJ, Bakr MM et al. Protocol for assessing maternal, environmental and epigenetic risk factors for dental caries in children. BMC Oral Health 2015; 15: 1–8.

There are 37 citations in total.

Details

Primary Language	English
Subjects	Health Care Administration
Journal Section	Original Research Articles
Authors	Gül Yıldız Telatar 0000-0001-5137-9282 Faruk Saydam 0000-0003-2358-8719
Project Number	TSA-2017-772
Publication Date	June 30, 2020
Submission Date	February 17, 2020
Published in Issue	Year 2020

Cite

EndNote	Yıldız Telatar G, Saydam F (June 1, 2020) Lack of Association between Enamel Gene Variants and Dental Caries in Adults. Cumhuriyet Dental Journal 23 2 96–106.

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