Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques
Abstract
as bone graft material. It has a high biocompatibility and biodegradability,
non-toxic, bacteriostatic, and has good adsorption properties. It also enhances
the proliferation and differentiation of the osteoblast cell cultures. However,
chitosan also has a weakness for cell adhesion and the lack of sufficient bone
formation capabilities. To improve it, we tried to develop a chitosan scaffolds
that combine with carbonate apatite (CA), which has excellent biocompatibility
properties of the tissues of the human body.
to make the carbonate apatite-chitosan scaffolds (CA-ChSs) and evaluate CA-ChSs
from the standpoint of cell proliferation using mesenchymal stem cells (MSCs).
lyophilization technique. ChSs containing chitosan powder was made by the
following procedure. 100, 150, 200, and 400 mg of chitosan powder was dissolved
in 5 ml of 2% acetic acid at room temperature. To make CA-ChSs, ChSs containing
200 mg of chitosan powder was chosen. Ultraviolet radiation was then performed
for 2 hours. CA-ChSs structure was observed by a scanning electron microscope
(SEM). Proliferation of MSCs in CA-ChSs evaluated at days 1, 3, 5 and 7.
200 mg of chitosan powder and 50 mg CA has a three-dimensional structure that
is porous and attachment powder CA in the pores and absorbance values were
increased from the examination day 1 until day 7.
may be candidates for bone graft material in tissue engineering techniques.
References
- Debnath, T. Ghosh, S. Potlapuvu, US. Kona, L, Kamaraju, SR. Sarkar, S. Gaddam, S. Chelluri, LK. Proliferation and differentiation potential of human adipose-derived stem cells grown on cytosan hydrogel. Plos One. 2015; 10(3): e0120803.
Abstract
References
- Debnath, T. Ghosh, S. Potlapuvu, US. Kona, L, Kamaraju, SR. Sarkar, S. Gaddam, S. Chelluri, LK. Proliferation and differentiation potential of human adipose-derived stem cells grown on cytosan hydrogel. Plos One. 2015; 10(3): e0120803.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Research Articles |
| Authors | |
| Publication Date | December 29, 2019 |
| Submission Date | July 23, 2019 |
| Published in Issue | Year 2019 |
Cited By
Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.
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