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Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats

Yıl 2023, Cilt: 13 Sayı: 4, 748 - 752, 29.12.2023
https://doi.org/10.33808/clinexphealthsci.1258371

Öz

Objective: The objective of this study was to investigate clinical effect of topically administered hyaluronic acid (HA) mouthwash on healing of secondary palatal wound in diabetic (D) rats.
Methods: 60 Wistar albino male rats were divided into D and non-diabetic (ND) groups. Diabetes was induced to 30 randomly selected rats by initially administering 110 mg/kg of nicotinamide intraperitoneally, followed by 15 min of intraperitoneal injection of 65 mg/kg of streptozotocin solution. 5 mm excisional wounds were made in the centre of the palate. After that, 6 animals from each group were sacrificed. Then, both groups were subdivided into two groups: 0.12% HA mouthwash and saline (S) (n=12 per group), depending on the agent to be administered to the wound area (WA). On days 7 and 14, six rats from each group were sacrificed, and the WAs were measured through photographic measurements utilizing Image J software.
Results: The WA decreased with time in each group (p<0.05). A significant difference was detected in the intergroup comparison of the WA on days 7 and 14 (p<0.05). On days 7 and 14, the smallest WA was observed in the ND-HA group, while the largest was in the D-S group (p<0.05). On day 14, the WA of both HA groups was similar (p>0.05) and smaller than that of both S groups (p<0.05).
Conclusion: Topical application of HA mouthwash effectively improved secondary wound healing and reduced WA in D and ND rats. Thus, topical application of HA can be used in diabetic palatal secondary wound healing.

Kaynakça

  • Alven S, Aderibigbe BA. Chitosan and cellulose-based hydrogels for wound management. Int J Mol Sci. 2020;21(24):9656. DOI: 10.3390/ijms21249656.
  • Chhabra S, Chhabra N, Kaur A, Gupta N. Wound healing concepts in clinical practice of OMFS. J Maxillofac Oral Surg. 2017;16(4):403-423. DOI: 10.1007/s12663-016-0880-z.
  • Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004;9:283-289. DOI: 10.2741/1184.
  • Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabet Med. 2006;23(6):594-608. DOI: 10.1111/j.1464-5491.2006.01773.x.
  • Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-29. DOI: 10.1177/0022034509359125.
  • American Diabetes A. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2014;37 (Suppl 1):S81-90. DOI: 10.2337/dc14-S081.
  • Yuksel EP, Ilkaya F, Yildiz L, Aydin F, Senturk N, Denizli H, Canturk T, Turanli AY. Effects of paroxetine on cutaneous wound healing in healthy and diabetic rats. Adv Skin Wound Care. 2014;27(5):216-21. DOI: 10.1097/01.ASW.0000445920.14039.64.
  • King L. Impaired wound healing in patients with diabetes. Nurs Stand. 2001;15(38):39-45. DOI: 10.7748/ns2001.06.15.38.39.c3039.
  • Ko KI, Sculean A, Graves DT. Diabetic wound healing in soft and hard oral tissues. Transl Res. 2021;236:72-86. DOI: 10.1016/j.trsl.2021.05.001.
  • Bartold PM, Wiebkin OW, Thonard JC. Glycosaminoglycans of human gingival epithelium and connective tissue. Connect Tissue Res. 1981;9(2):99-106. DOI: 10.3109/03008208109160247.
  • Yildirim S, Ozener HO, Dogan B, Kuru B. Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner-masked, randomized, controlled clinical trial. J Periodontol. 2018;89(1):36-45. DOI: 10.1902/jop.2017.170105.
  • Chen RF, Wang CT, Chen YH, Chien CM, Lin SD, Lai CS, Wang CJ, Kuo YR. Hyaluronic acid-povidone-iodine compound facilitates diabetic wound healing in a streptozotocin-induced diabetes rodent model. Plast Reconstr Surg. 2019;143(5):1371-1382. DOI: 10.1097/PRS.0000000000005504.
  • Brown JA. The role of hyaluronic acid in wound healing's proliferative phase. J Wound Care. 2004;13(2):48-51. DOI: 10.12968/jowc.2004.13.2.26573.
  • Aya KL, Stern R. Hyaluronan in wound healing: rediscovering a major player. Wound Repair Regen. 2014;22(5):579-93. DOI: 10.1111/wrr.12214.
  • Neuman MG, Nanau RM, Oruna-Sanchez L, Coto G. Hyaluronic acid and wound healing. J Pharm Pharm Sci. 2015;18(1):53-60. DOI: 10.18433/j3k89d.
  • Frenkel JS. The role of hyaluronan in wound healing. Int Wound J. 2014;11(2):159-63. DOI: 10.1111/j.1742-481X.2012.01057.x.
  • Fouda MMG, Abdel-Mohsen AM, Ebaid H, Hassan I, Al-Tamimi J, Abdel-Rahman RM, Metwalli A, Alhazza I, Rady A, El-Faham A, Jancar J. Wound healing of different molecular weight of hyaluronan; in-vivo study. Int J Biol Macromol. 2016;89:582-591. DOI: 10.1016/j.ijbiomac.2016.05.021.
  • Khedr EM, Alkady EA, El-Hammady DH, Khalifa FA, bin-Humam S. Repetitive lumbosacral nerve magnetic stimulation improves bladder dysfunction due to lumbosacral nerve injury: a pilot randomized controlled study. Neurorehabil Neural Repair. 2011;25(6):570-576. DOI: 10.1177/1545968311400091.
  • Taskan MM, Balci Yuce H, Karatas O, Gevrek F, Isiker Kara G, Celt M, Sirma Taskan E. Hyaluronic acid with antioxidants improve wound healing in rats. Biotech Histochem. 2021;96(7):536-545. DOI: 10.1080/10520295.2020.1832255.
  • Parmar KM, Shende PR, Katare N, Dhobi M, Prasad SK. Wound healing potential of Solanum xanthocarpum in streptozotocin-induced diabetic rats. J Pharm Pharmacol. 2018;70(10):1389-1400. DOI: 10.1111/jphp.12975.
  • Hammad HM, Hammad MM, Abdelhadi IN, Khalifeh MS. Effects of topically applied agents on intra-oral wound healing in a rat model: a clinical and histomorphometric study. Int J Dent Hyg. 2011;9(1):9-16. DOI: 10.1111/j.1601-5037.2009.00410.x.
  • Bainbridge P. Wound healing and the role of fibroblasts. J Wound Care. 2013;22(8):407-408, 410-12. DOI: 10.12968/jowc.2013.22.8.407.
  • Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res. 2012;49(1):35-43. DOI: 10.1159/000339613.
  • Hassan A, Ahmed E, Ghalwash D, Elarab AE. Clinical comparison of MEBO and hyaluronic acid gel in the management of pain after free gingival graft harvesting: a randomized clinical trial. Int J Dent. 2021;2021:2548665. DOI: 10.1155/2021/2548665.
  • Lee JH, Lee KE, Nam OH, Chae YK, Lee MH, Kweon DK, Kim MS, Lee HS, Choi SC. Orodispersible hyaluronic acid film delivery for oral wound healing in rats. J Dent Sci. 2022;17(4):1595-1603. DOI: 10.1016/j.jds.2022.04.004.
  • Dahiya P, Kamal R. Hyaluronic acid: a boon in periodontal therapy. N Am J Med Sci. 2013;5(5):309-315. DOI: 10.4103/1947-2714.112473.
Yıl 2023, Cilt: 13 Sayı: 4, 748 - 752, 29.12.2023
https://doi.org/10.33808/clinexphealthsci.1258371

Öz

Kaynakça

  • Alven S, Aderibigbe BA. Chitosan and cellulose-based hydrogels for wound management. Int J Mol Sci. 2020;21(24):9656. DOI: 10.3390/ijms21249656.
  • Chhabra S, Chhabra N, Kaur A, Gupta N. Wound healing concepts in clinical practice of OMFS. J Maxillofac Oral Surg. 2017;16(4):403-423. DOI: 10.1007/s12663-016-0880-z.
  • Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004;9:283-289. DOI: 10.2741/1184.
  • Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabet Med. 2006;23(6):594-608. DOI: 10.1111/j.1464-5491.2006.01773.x.
  • Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-29. DOI: 10.1177/0022034509359125.
  • American Diabetes A. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2014;37 (Suppl 1):S81-90. DOI: 10.2337/dc14-S081.
  • Yuksel EP, Ilkaya F, Yildiz L, Aydin F, Senturk N, Denizli H, Canturk T, Turanli AY. Effects of paroxetine on cutaneous wound healing in healthy and diabetic rats. Adv Skin Wound Care. 2014;27(5):216-21. DOI: 10.1097/01.ASW.0000445920.14039.64.
  • King L. Impaired wound healing in patients with diabetes. Nurs Stand. 2001;15(38):39-45. DOI: 10.7748/ns2001.06.15.38.39.c3039.
  • Ko KI, Sculean A, Graves DT. Diabetic wound healing in soft and hard oral tissues. Transl Res. 2021;236:72-86. DOI: 10.1016/j.trsl.2021.05.001.
  • Bartold PM, Wiebkin OW, Thonard JC. Glycosaminoglycans of human gingival epithelium and connective tissue. Connect Tissue Res. 1981;9(2):99-106. DOI: 10.3109/03008208109160247.
  • Yildirim S, Ozener HO, Dogan B, Kuru B. Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner-masked, randomized, controlled clinical trial. J Periodontol. 2018;89(1):36-45. DOI: 10.1902/jop.2017.170105.
  • Chen RF, Wang CT, Chen YH, Chien CM, Lin SD, Lai CS, Wang CJ, Kuo YR. Hyaluronic acid-povidone-iodine compound facilitates diabetic wound healing in a streptozotocin-induced diabetes rodent model. Plast Reconstr Surg. 2019;143(5):1371-1382. DOI: 10.1097/PRS.0000000000005504.
  • Brown JA. The role of hyaluronic acid in wound healing's proliferative phase. J Wound Care. 2004;13(2):48-51. DOI: 10.12968/jowc.2004.13.2.26573.
  • Aya KL, Stern R. Hyaluronan in wound healing: rediscovering a major player. Wound Repair Regen. 2014;22(5):579-93. DOI: 10.1111/wrr.12214.
  • Neuman MG, Nanau RM, Oruna-Sanchez L, Coto G. Hyaluronic acid and wound healing. J Pharm Pharm Sci. 2015;18(1):53-60. DOI: 10.18433/j3k89d.
  • Frenkel JS. The role of hyaluronan in wound healing. Int Wound J. 2014;11(2):159-63. DOI: 10.1111/j.1742-481X.2012.01057.x.
  • Fouda MMG, Abdel-Mohsen AM, Ebaid H, Hassan I, Al-Tamimi J, Abdel-Rahman RM, Metwalli A, Alhazza I, Rady A, El-Faham A, Jancar J. Wound healing of different molecular weight of hyaluronan; in-vivo study. Int J Biol Macromol. 2016;89:582-591. DOI: 10.1016/j.ijbiomac.2016.05.021.
  • Khedr EM, Alkady EA, El-Hammady DH, Khalifa FA, bin-Humam S. Repetitive lumbosacral nerve magnetic stimulation improves bladder dysfunction due to lumbosacral nerve injury: a pilot randomized controlled study. Neurorehabil Neural Repair. 2011;25(6):570-576. DOI: 10.1177/1545968311400091.
  • Taskan MM, Balci Yuce H, Karatas O, Gevrek F, Isiker Kara G, Celt M, Sirma Taskan E. Hyaluronic acid with antioxidants improve wound healing in rats. Biotech Histochem. 2021;96(7):536-545. DOI: 10.1080/10520295.2020.1832255.
  • Parmar KM, Shende PR, Katare N, Dhobi M, Prasad SK. Wound healing potential of Solanum xanthocarpum in streptozotocin-induced diabetic rats. J Pharm Pharmacol. 2018;70(10):1389-1400. DOI: 10.1111/jphp.12975.
  • Hammad HM, Hammad MM, Abdelhadi IN, Khalifeh MS. Effects of topically applied agents on intra-oral wound healing in a rat model: a clinical and histomorphometric study. Int J Dent Hyg. 2011;9(1):9-16. DOI: 10.1111/j.1601-5037.2009.00410.x.
  • Bainbridge P. Wound healing and the role of fibroblasts. J Wound Care. 2013;22(8):407-408, 410-12. DOI: 10.12968/jowc.2013.22.8.407.
  • Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res. 2012;49(1):35-43. DOI: 10.1159/000339613.
  • Hassan A, Ahmed E, Ghalwash D, Elarab AE. Clinical comparison of MEBO and hyaluronic acid gel in the management of pain after free gingival graft harvesting: a randomized clinical trial. Int J Dent. 2021;2021:2548665. DOI: 10.1155/2021/2548665.
  • Lee JH, Lee KE, Nam OH, Chae YK, Lee MH, Kweon DK, Kim MS, Lee HS, Choi SC. Orodispersible hyaluronic acid film delivery for oral wound healing in rats. J Dent Sci. 2022;17(4):1595-1603. DOI: 10.1016/j.jds.2022.04.004.
  • Dahiya P, Kamal R. Hyaluronic acid: a boon in periodontal therapy. N Am J Med Sci. 2013;5(5):309-315. DOI: 10.4103/1947-2714.112473.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Periodontoloji
Bölüm Articles
Yazarlar

Ezgi Akçe Bu kişi benim 0000-0002-3879-7073

Sibel Demirci Delipınar 0000-0002-4546-4664

Leyla Kuru 0000-0002-7599-2409

Hatice Selin Yıldırım 0000-0001-5650-3107

Yayımlanma Tarihi 29 Aralık 2023
Gönderilme Tarihi 20 Mart 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 13 Sayı: 4

Kaynak Göster

APA Akçe, E., Demirci Delipınar, S., Kuru, L., Yıldırım, H. S. (2023). Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats. Clinical and Experimental Health Sciences, 13(4), 748-752. https://doi.org/10.33808/clinexphealthsci.1258371
AMA Akçe E, Demirci Delipınar S, Kuru L, Yıldırım HS. Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats. Clinical and Experimental Health Sciences. Aralık 2023;13(4):748-752. doi:10.33808/clinexphealthsci.1258371
Chicago Akçe, Ezgi, Sibel Demirci Delipınar, Leyla Kuru, ve Hatice Selin Yıldırım. “Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats”. Clinical and Experimental Health Sciences 13, sy. 4 (Aralık 2023): 748-52. https://doi.org/10.33808/clinexphealthsci.1258371.
EndNote Akçe E, Demirci Delipınar S, Kuru L, Yıldırım HS (01 Aralık 2023) Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats. Clinical and Experimental Health Sciences 13 4 748–752.
IEEE E. Akçe, S. Demirci Delipınar, L. Kuru, ve H. S. Yıldırım, “Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats”, Clinical and Experimental Health Sciences, c. 13, sy. 4, ss. 748–752, 2023, doi: 10.33808/clinexphealthsci.1258371.
ISNAD Akçe, Ezgi vd. “Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats”. Clinical and Experimental Health Sciences 13/4 (Aralık 2023), 748-752. https://doi.org/10.33808/clinexphealthsci.1258371.
JAMA Akçe E, Demirci Delipınar S, Kuru L, Yıldırım HS. Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats. Clinical and Experimental Health Sciences. 2023;13:748–752.
MLA Akçe, Ezgi vd. “Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats”. Clinical and Experimental Health Sciences, c. 13, sy. 4, 2023, ss. 748-52, doi:10.33808/clinexphealthsci.1258371.
Vancouver Akçe E, Demirci Delipınar S, Kuru L, Yıldırım HS. Evaluation of the Clinical Effect of Hyaluronic Acid Mouthwash on Palatal Secondary Wound Healing in Diabetic Rats. Clinical and Experimental Health Sciences. 2023;13(4):748-52.

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