Effects of a new combination folkloric medicinal plant extract on bone formation in orthopedically expanded suture in rats

Şeref Ezirganlı; Hakan Özdemir; Muhammed Birlik; Oguz Kazancioglu; Sertaç Aksakallı; Mukaddes Eşrefoğlu

doi:10.34084/bshr.1284711

Araştırma Makalesi

Yeni bir kombinasyon folklorik şifalı bitki ekstraktının sıçanlarda ortopedik olarak genişletilmiş sütürde kemik oluşumu üzerindeki etkileri

Yıl 2023, Cilt: 7 Sayı: 2, 106 - 113, 30.08.2023

Şeref Ezirganlı Hakan Özdemir Muhammed Birlik Oguz Kazancioglu Sertaç Aksakallı Mukaddes Eşrefoğlu

https://doi.org/10.34084/bshr.1284711

Öz

Amaç: Bu çalışmanın amacı, sıçanlarda premaksiller sütür ekspansiyonunda yeni bir kombinasyon folkrorik tıbbi bitki ekstratının kemik iyileşmesi üzerindeki histolojik etkilerinin değerlendirmektir.
Materyal ve metod: Bu çalışmada otuz erkek Spraque-Dawley sıçanı kullanılmıştır. Çalışma her biri eşit olmak üzere 10 sıçandan oluşan bir kontrol iki deney grubuna ayrılmıştır. Sıçanlar sarmal yaylar ile premaksiller sütür genişlemesine tabi tutulmuştur. Tek genişleme grubu kontrol grubu olarak (Group A) tanımlanmıştır. Deney grubu OstokinPlus-10 (Group B) ve OstokinPlus-20 (Group C) olarak tanımlanmıştır. Deney gruplarında, 10 ve 20 ml/kg OstokinPlus bitkileri, çalışma süresi boyunca orogastrik tüp kullanılarak genişletildikten sonra sistemik olarak uygulanmıştır. Yaylar yerleştirilmiş ve 30 SN kuvveti sağlayacak şekilde etkinleştirilmiştir. 5 gün sonra, yaylar çıkarılmış ve kısa uzunluklarda dikdörtgen tespit teli ile değiştirilmiştir. Diş ayrımı 15 gün sürdürülmüştür. 15 günlük bir konsolidasyon periyodundan sonra, hayvanlara ötenazi uygulanmış ve midpalatal sütur kıkırdağını içeren maksiller kemik cerrahi olarak çıkarılmıştır. Örnekler, rejenere kemiğin histomorfometrik değerlendirmesi için hazırlanmıştır.
Bulgular: Aktive heliks yayı uygulamasının ardından midpalatal sütur başarılı bir şekilde ayrılmıştır. Ayrılan premaksiller sütur yeni kemik ve organize olmayan fibröz dokular ile dolmuştur. Yeni oluşan kemik yüzdesi ve kemik alanı arasında önemli farklılıklar bulunmuştur (p< 0.05). Araştırılan parametreler için Group B ve Group C, Group A daha iyi sonuçlar ortaya koymuştur.
Sonuç: OstokinPlus ekstaratı premaksiller sütur genişlemesi esnasında kemik iyileşmesi ve formasyonunu olumlu yönde etkilemiştir.

Anahtar Kelimeler

Hızlı maksiller genişleme, OstokinPlus bitkisi, kemik rejenerasyonu, histomorfometri

Destekleyen Kurum

herhangi bir kurum desteği bulunmamaktadır

Kaynakça

1. Saito S, Shimizu N. Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod and Dentofac Orthop. 1997; 111: 525-532.
2. Sawada M, Shimizu N. Stimulation of bone formation in the expanding mid-palatal suture by transforming growth factor-beta 1 in the rat. Eur J Orthod. 1996;18:169-179.
3. Altan BA, Kara IM, Nalcaci R, Ozan F, Erdogan SM, Ozkut MM, et al. Systemic propolis stimulates new bone formation at the expanded suture: a histomorphometric study. Angle Orthod. 2013; 83: 286-291.
4. Gurel HG, Memili B, Erkan M, Sukurica Y. Long-term effects of rapid maxillary expansion followed by fixed appliances. Angle Orthod. 2010; 80: 5-9.
5. Petrick S, Hothan T, Hietschold V, Schneider M, Harzer W, Tausche E. Bone density of the midpalatal suture 7 months after surgically assisted rapid palatal expansion in adults. Am J Orthod and Dentofac Orthop. 2011; 139: S109-116.
6. Uysal T, Amasyali M, Enhos S, Karslioglu Y, Yilmaz F, Gunhan O. Effect of periosteal stimulation therapy on bone formation in orthopedically expanded suture in rats. Orthod Craniofac Res. 2010; 13: 89-95.
7. Uysal T, Amasyali M, Enhos S, Sonmez MF, Sagdic D. Effect of ED-71, a New Active Vitamin D Analog, on Bone Formation in an Orthopedically Expanded Suture in Rats. A Histomorphometric Study. Eur J Orthod. 2009; 3: 165-172.
8. Lagravere MO, Major PW, Flores-Mir C. Long-term skeletal changes with rapid maxillary expansion: a systematic review. Angle Orthod. 2005; 75: 1046-1052.
9. Baccetti T, Franchi L, Cameron CG, McNamara JA, Jr. Treatment timing for rapid maxillary expansion. Angle Orthod. 2001; 71: 343-350.
10. Ekizer A, Uysal T, Guray E, Yuksel Y. Light-emitting diode photobiomodulation: effect on bone formation in orthopedically expanded suture in rats--early bone changes. Lasers Med Sci. 2013; 28: 1263-1270. 11. Uysal T, Gorgulu S, Yagci A, Karslioglu Y, Gunhan O, Sagdic D. Effect of resveratrol on bone formation in the expanded inter-premaxillary suture: early bone changes. Orthod Craniofac Res. 2011; 14: 80-87.
12. Uysal T, Amasyali M, Olmez H, Enhos S, Karslioglu Y, Gunhan O. Effect of vitamin C on bone formation in the expanded inter-premaxillary suture. Early bone changes. J Orofac Orthop. 2011; 72: 290-300.
13. Dai L, Wu H, Yu S, Zhao H, Xue L, Xu M, et al. Effects of OsteoKing on osteoporotic rabbits. Mol Med Rep 2015;12:1066-74.
14. Zhao HB, Hu M, Liang HS. Experimental study on osteoking in promoting gene expression of core binding factor alpha 1 in necrotic femoral head of rabbits. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006; 26: 1003-1006.
15. Ezirganli S, Kazancioglu HO, Mihmanli A, Aydin MS, Sharifov R, Alkan A. The effect of local simvastatin application on critical size defects in the diabetic rats. Clin Oral Implants Res 2014; 25: 969-976.
16. Takahashi O. Histological investigations on the effect of interrupted expansion force applied to the midpalatal suture in the rat. Nichidai Koko Kagaku 1990; 16: 212-236.
17. Rosa CB, Habib FA, de Araujo TM, Aragao JS, Gomes RS, Barbosa AF, et al. Effect of the laser and light-emitting diode (LED) phototherapy on midpalatal suture bone formation after rapid maxilla expansion: a Raman spectroscopy analysis. Lasers Med Sci. 2014 ; 29(3): 859-867.
18. Arat ZM, Gokalp H, Atasever T, Turkkahraman H. 99mTechnetium-labeled methylene diphosphonate uptake in maxillary bone during and after rapid maxillary expansion. Angle Orthod. 2003; 73: 545-549.
19. Braun S, Bottrel JA, Lee KG, Lunazzi JJ, Legan HL. The biomechanics of rapid maxillary sutural expansion. Am J Orthod and Dentofac Orthop. 2000; 118: 257-261.
20. da Silva AP, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AL, et al. Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci. 2012; 27: 777-783.
21. Duygulu F, Yakan B, Karaoglu S, Kutlubay R, Karahan OI, Ozturk A. The effect of zymosan and the protective effect of various antioxidants on fracture healing in rats. Arch Orthop Trauma Surg 2007; 127: 493-501.
22. Lee K, Sugiyama H, Imoto S, Tanne K. Effects of bisphosphonate on the remodeling of rat sagittal suture after rapid expansion. Angle Orthod. 2001; 71: 265-273.
23. Jiang Y, Liu HX, Guo JJ, Tang GH, Qian YF. Stimulation of bone formation in the expanding premaxillary suture with a GSK-3beta inhibitor. Oral Diseases.2013; 19: 73-79.
24. Tang GH, Xu J, Chen RJ, Qian YF, Shen G. Lithium delivery enhances bone growth during midpalatal expansion. J Dent Res. 2011; 90: 336-340.
25. Kobayashi ET, Hashimoto F, Kobayashi Y, Sakai E, Miyazaki Y, Kamiya T, et al. Force-induced rapid changes in cell fate at midpalatal suture cartilage of growing rats. J Dent Res. 1999; 78: 1495-1504.
26. Zhong WJ, Zhang WB, Ma JQ, Wang H, Pan YC, Wang L. Periostin-like-factor-induced bone formation within orthopedic maxillary expansion. Orthod Craniofac Res. 2011; 14: 198-205.
27. Kang SC, Kim HJ, Kim MH. Effects of Astragalus membranaceus with supplemental calcium on bone mineral density and bone metabolism in calcium-deficient ovariectomized rats. Biol Trace Elem Res. 2013; 151: 68-74.
28. Wegiel B, Persson JL. Effect of a novel botanical agent Drynol Cibotin on human osteoblast cells and implications for osteoporosis: promotion of cell growth, calcium uptake and collagen production. Phytother Res. 2010; 24 (Suppl 2): S139-147.
29. Wang P, Wei X, Zhang F, Yang K, Qu C, Luo H, et al. Ginsenoside Rg1 of Panax ginseng stimulates the proliferation, odontogenic/osteogenic differentiation and gene expression profiles of human dental pulp stem cells. Phytomedicine. 2014; 21: 177-183.
30. Avsar U, Karakus E, Halici Z, Bayir Y, Bilen H, Aydin A, et al. Prevention of bone loss by Panax ginseng in a rat model of inflammation-induced bone loss. Cell Mol Biol. 2013; 59 Suppl: OL1835-1841.
31. Lee YS, Choi CW, Kim JJ, Ganapathi A, Udayakumar R, Kim SC. Determination of mineral content in methanolic safflower (Carthamus tinctorius L.) seed extract and its effect on osteoblast markers. Int J Mol Sci. 2009; 10: 292-305.
32. Umar Hussain AA, Muhammad Farhan. Etiology and treatment of midline diastema: A review of literature. Pakistan Orthodontic Journal. 2013; 5(1): 27-33.
33. Bennamane M, Zeror S, Aribi-Zouioueche L. Asymmetric Reduction of Ketones by Biocatalysis Using Clementine Mandarin (Citrus reticulata) Fruit Grown in Annaba or by Ruthenium Catalysis for Access to Both Enantiomers. Chirality. 2015; 27(3): 205-210
34. Lee YC, Cheng TH, Lee JS, Chen JH, Liao YC, Fong Y, et al. Nobiletin, a citrus flavonoid, suppresses invasion and migration involving FAK/PI3K/Akt and small GTPase signals in human gastric adenocarcinoma AGS cells. Mol Cell Biochem. 2011; 347: 103-115.
35. Zhu X, Luo F, Zheng Y, Zhang J, Huang J, Sun C, et al. Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901. Int J Mol Sci. 2013; 14: 8684-8897.
36. Hu M, Zhao H, Dong X, Luo D, Zhou X. General and light microscope observation on histological changes of femoral heads between SANFH rabbit animal models and it were intervened by Osteoking. Zhongguo Zhong Yao Za Zhi. 2010; 35: 2912-2916.

Effects of a new combination folkloric medicinal plant extract on bone formation in orthopedically expanded suture in rats

Yıl 2023, Cilt: 7 Sayı: 2, 106 - 113, 30.08.2023

Şeref Ezirganlı Hakan Özdemir Muhammed Birlik Oguz Kazancioglu Sertaç Aksakallı Mukaddes Eşrefoğlu

https://doi.org/10.34084/bshr.1284711

Öz

Objectives. The aim of this study was to evaluate histological effects of a new combination folkloric medicinal plant extract on bone healing in premaxillary suture expansion in rats.
Materials and methods. Thirty male Sprague–Dawley rats were used in this study. Rats were evenly divided into three groups (one control and two experimental groups) of ten each. The animals were subjected to premaxillary suture expansion by helix springs. The only expansion group is defined as the control group (Group A). The experimental groups are defined as OstokinPlus-10 (Group B) and OstokinPlus-20 (Group C). In the experimental groups, 10 and 20 ml/kg OstokinPlus herbals were applied systemically after the expansion by use of an orogastric tube during the time of study. The springs were placed and activated to deliver a 30 cN force. After 5 days, the springs were removed and replaced with short lengths of rectangular retaining wire. Tooth separation was maintained for 15 days. After a consolidation period of 15 days, the animals were euthanatized and the maxillary bone containing the midpalatal suture cartilage was surgically removed. The specimens were prepared for histomorphometric assessment of the regenerated bone.
Results. The midpalatal suture was successfully distracted following application of the activated helix spring. The distracted premaxillary suture was filled with new bone formation and unorganized fibrous tissues. Newly formed bone percentage and the bone area were found to have significant differences (p< 0.05). For investigated parameters, Group B and Group C revealed more positive results than Group A.
Conclusion: OstokinPlus herbal had positive effects on bone healing and formation during premaxillary suture expansion

Anahtar Kelimeler

Rapid maxillary expansion, OstokinPlus herbal, Bone regeneration, Histomorphometry

Kaynakça

1. Saito S, Shimizu N. Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod and Dentofac Orthop. 1997; 111: 525-532.
2. Sawada M, Shimizu N. Stimulation of bone formation in the expanding mid-palatal suture by transforming growth factor-beta 1 in the rat. Eur J Orthod. 1996;18:169-179.
3. Altan BA, Kara IM, Nalcaci R, Ozan F, Erdogan SM, Ozkut MM, et al. Systemic propolis stimulates new bone formation at the expanded suture: a histomorphometric study. Angle Orthod. 2013; 83: 286-291.
4. Gurel HG, Memili B, Erkan M, Sukurica Y. Long-term effects of rapid maxillary expansion followed by fixed appliances. Angle Orthod. 2010; 80: 5-9.
5. Petrick S, Hothan T, Hietschold V, Schneider M, Harzer W, Tausche E. Bone density of the midpalatal suture 7 months after surgically assisted rapid palatal expansion in adults. Am J Orthod and Dentofac Orthop. 2011; 139: S109-116.
6. Uysal T, Amasyali M, Enhos S, Karslioglu Y, Yilmaz F, Gunhan O. Effect of periosteal stimulation therapy on bone formation in orthopedically expanded suture in rats. Orthod Craniofac Res. 2010; 13: 89-95.
7. Uysal T, Amasyali M, Enhos S, Sonmez MF, Sagdic D. Effect of ED-71, a New Active Vitamin D Analog, on Bone Formation in an Orthopedically Expanded Suture in Rats. A Histomorphometric Study. Eur J Orthod. 2009; 3: 165-172.
8. Lagravere MO, Major PW, Flores-Mir C. Long-term skeletal changes with rapid maxillary expansion: a systematic review. Angle Orthod. 2005; 75: 1046-1052.
9. Baccetti T, Franchi L, Cameron CG, McNamara JA, Jr. Treatment timing for rapid maxillary expansion. Angle Orthod. 2001; 71: 343-350.
10. Ekizer A, Uysal T, Guray E, Yuksel Y. Light-emitting diode photobiomodulation: effect on bone formation in orthopedically expanded suture in rats--early bone changes. Lasers Med Sci. 2013; 28: 1263-1270. 11. Uysal T, Gorgulu S, Yagci A, Karslioglu Y, Gunhan O, Sagdic D. Effect of resveratrol on bone formation in the expanded inter-premaxillary suture: early bone changes. Orthod Craniofac Res. 2011; 14: 80-87.
12. Uysal T, Amasyali M, Olmez H, Enhos S, Karslioglu Y, Gunhan O. Effect of vitamin C on bone formation in the expanded inter-premaxillary suture. Early bone changes. J Orofac Orthop. 2011; 72: 290-300.
13. Dai L, Wu H, Yu S, Zhao H, Xue L, Xu M, et al. Effects of OsteoKing on osteoporotic rabbits. Mol Med Rep 2015;12:1066-74.
14. Zhao HB, Hu M, Liang HS. Experimental study on osteoking in promoting gene expression of core binding factor alpha 1 in necrotic femoral head of rabbits. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006; 26: 1003-1006.
15. Ezirganli S, Kazancioglu HO, Mihmanli A, Aydin MS, Sharifov R, Alkan A. The effect of local simvastatin application on critical size defects in the diabetic rats. Clin Oral Implants Res 2014; 25: 969-976.
16. Takahashi O. Histological investigations on the effect of interrupted expansion force applied to the midpalatal suture in the rat. Nichidai Koko Kagaku 1990; 16: 212-236.
17. Rosa CB, Habib FA, de Araujo TM, Aragao JS, Gomes RS, Barbosa AF, et al. Effect of the laser and light-emitting diode (LED) phototherapy on midpalatal suture bone formation after rapid maxilla expansion: a Raman spectroscopy analysis. Lasers Med Sci. 2014 ; 29(3): 859-867.
18. Arat ZM, Gokalp H, Atasever T, Turkkahraman H. 99mTechnetium-labeled methylene diphosphonate uptake in maxillary bone during and after rapid maxillary expansion. Angle Orthod. 2003; 73: 545-549.
19. Braun S, Bottrel JA, Lee KG, Lunazzi JJ, Legan HL. The biomechanics of rapid maxillary sutural expansion. Am J Orthod and Dentofac Orthop. 2000; 118: 257-261.
20. da Silva AP, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AL, et al. Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci. 2012; 27: 777-783.
21. Duygulu F, Yakan B, Karaoglu S, Kutlubay R, Karahan OI, Ozturk A. The effect of zymosan and the protective effect of various antioxidants on fracture healing in rats. Arch Orthop Trauma Surg 2007; 127: 493-501.
22. Lee K, Sugiyama H, Imoto S, Tanne K. Effects of bisphosphonate on the remodeling of rat sagittal suture after rapid expansion. Angle Orthod. 2001; 71: 265-273.
23. Jiang Y, Liu HX, Guo JJ, Tang GH, Qian YF. Stimulation of bone formation in the expanding premaxillary suture with a GSK-3beta inhibitor. Oral Diseases.2013; 19: 73-79.
24. Tang GH, Xu J, Chen RJ, Qian YF, Shen G. Lithium delivery enhances bone growth during midpalatal expansion. J Dent Res. 2011; 90: 336-340.
25. Kobayashi ET, Hashimoto F, Kobayashi Y, Sakai E, Miyazaki Y, Kamiya T, et al. Force-induced rapid changes in cell fate at midpalatal suture cartilage of growing rats. J Dent Res. 1999; 78: 1495-1504.
26. Zhong WJ, Zhang WB, Ma JQ, Wang H, Pan YC, Wang L. Periostin-like-factor-induced bone formation within orthopedic maxillary expansion. Orthod Craniofac Res. 2011; 14: 198-205.
27. Kang SC, Kim HJ, Kim MH. Effects of Astragalus membranaceus with supplemental calcium on bone mineral density and bone metabolism in calcium-deficient ovariectomized rats. Biol Trace Elem Res. 2013; 151: 68-74.
28. Wegiel B, Persson JL. Effect of a novel botanical agent Drynol Cibotin on human osteoblast cells and implications for osteoporosis: promotion of cell growth, calcium uptake and collagen production. Phytother Res. 2010; 24 (Suppl 2): S139-147.
29. Wang P, Wei X, Zhang F, Yang K, Qu C, Luo H, et al. Ginsenoside Rg1 of Panax ginseng stimulates the proliferation, odontogenic/osteogenic differentiation and gene expression profiles of human dental pulp stem cells. Phytomedicine. 2014; 21: 177-183.
30. Avsar U, Karakus E, Halici Z, Bayir Y, Bilen H, Aydin A, et al. Prevention of bone loss by Panax ginseng in a rat model of inflammation-induced bone loss. Cell Mol Biol. 2013; 59 Suppl: OL1835-1841.
31. Lee YS, Choi CW, Kim JJ, Ganapathi A, Udayakumar R, Kim SC. Determination of mineral content in methanolic safflower (Carthamus tinctorius L.) seed extract and its effect on osteoblast markers. Int J Mol Sci. 2009; 10: 292-305.
32. Umar Hussain AA, Muhammad Farhan. Etiology and treatment of midline diastema: A review of literature. Pakistan Orthodontic Journal. 2013; 5(1): 27-33.
33. Bennamane M, Zeror S, Aribi-Zouioueche L. Asymmetric Reduction of Ketones by Biocatalysis Using Clementine Mandarin (Citrus reticulata) Fruit Grown in Annaba or by Ruthenium Catalysis for Access to Both Enantiomers. Chirality. 2015; 27(3): 205-210
34. Lee YC, Cheng TH, Lee JS, Chen JH, Liao YC, Fong Y, et al. Nobiletin, a citrus flavonoid, suppresses invasion and migration involving FAK/PI3K/Akt and small GTPase signals in human gastric adenocarcinoma AGS cells. Mol Cell Biochem. 2011; 347: 103-115.
35. Zhu X, Luo F, Zheng Y, Zhang J, Huang J, Sun C, et al. Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901. Int J Mol Sci. 2013; 14: 8684-8897.
36. Hu M, Zhao H, Dong X, Luo D, Zhou X. General and light microscope observation on histological changes of femoral heads between SANFH rabbit animal models and it were intervened by Osteoking. Zhongguo Zhong Yao Za Zhi. 2010; 35: 2912-2916.

Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Diş Hekimliği
Bölüm	Araştırma Makalesi
Yazarlar	Şeref Ezirganlı 0000-0001-9591-1373 Hakan Özdemir 0000-0001-9550-1999 Muhammed Birlik Bu kişi benim 0000-0002-9182-0842 Oguz Kazancioglu 0000-0002-9530-7456 Sertaç Aksakallı 0000-0002-1916-8108 Mukaddes Eşrefoğlu 0000-0003-3380-1480
Erken Görünüm Tarihi	3 Eylül 2023
Yayımlanma Tarihi	30 Ağustos 2023
Kabul Tarihi	8 Haziran 2023
Yayımlandığı Sayı	Yıl 2023 Cilt: 7 Sayı: 2

Kaynak Göster

AMA	Ezirganlı Ş, Özdemir H, Birlik M, Kazancioglu O, Aksakallı S, Eşrefoğlu M. Effects of a new combination folkloric medicinal plant extract on bone formation in orthopedically expanded suture in rats. J Biotechnol and Strategic Health Res. Ağustos 2023;7(2):106-113. doi:10.34084/bshr.1284711

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