Investigation of the Effect of Disinfectant and Antioxidant Intrauterine "Anionic Oxygen" Application on Fertility after Artificial Insemination in Dairy Cows
Öz
In the study, a total of 30 clinically healthy fertile Holstein cows (aged 3-8 years old; with 500-700 kg average live weight and 28 litres average daily milk) were used in a private dairy farm in Milas district of Muğla province. Randomly selected cows were divided into two groups (control and treatment) (n=15 cows/group) with almost similar body condition scores (BCS) (as all having approximately BCS of 2.75±0.25 units, 1-5 scale). Cows were monitored for natural oestrus, especially during the 2 months postpartum. Control cows (n=15) showing oestrus were inseminated (throughout the 3 consecutive occasions at maximum) by routine AI via recto-vaginal intrauterine route using frozen bull semen (0.25 ml dose; 7.5 x 106 total motile sperm) and thawing at 37 °C for 30 seconds. Inseminations were carried out for 7 months (from December 2021 to June 2022). For the treatment group, the cows were given the antimicrobial agent (UB20 O8®) intrauterine 30 minutes after each AI. The disinfectant dose, given to the corpus uteri region after the AI, was used as a total of 50 ml mixture (40 ml warm saline and 10 ml disinfectant). Throughout the study, animals were routinely given a farm-specific "dairy cow" diet (with 23% protein/concentrated feed). In addition, cows were provided regularly with clean drinking water, shade, and free movement in paddocks. Total pregnancy rates of cows and the 'pregnancy index' (the number of inseminations per pregnancy) of 35-60-day post-insemination as checked via ultrasound examination were analysed statistically by using Chi-Square and Wilcoxon tests. The possible difference between the groups based on pregnancy rate and pregnancy index data was considered significant at the P<0.05 level. According to the results obtained, numerical differences observed due to local treatment after all insemination periods were not statistically significant (P>0.05). Pregnancy rates following the 1st, 2nd, and 3rd inseminations in the treatment and control groups were 60.00% vs. 46.66%, 86.66% vs. 66.66%, and 93.33% vs. 80.00%, respectively. The total pregnancy indexes in the groups were 1.57 (14 pregnancies/22 inseminations; treatment) and 2.25 (12 pregnancies/27 inseminations; control). Briefly, it was observed that the pregnancy rate and pregnancy index in the treatment group were consistently but only numerically higher than those in the control group, indicating that anionic oxygen could be an effective alternative for intrauterine treatment leading to high fertility early postpartum.
Anahtar Kelimeler
Destekleyen Kurum
TÜBİTAK-Ankara
Proje Numarası
2209 A, Proje No 1919B012109561 (2021/2. Dönem)
Teşekkür
We thank TÜBİTAK Directorate (Ankara-TÜRKİYE) for their guidance and support.
Kaynakça
- Apaydın Yıldırım B. 2021. Anti-cancer, antiproliferative activity of active anionic H208 oxygen solution on HCT-116 cancer cells. World J Adv Res Rev, 12(02): 179-184.
- BAYNOVA Catalogue. 2021. Ultra Biyosit enzimli aktif anyonik oktaoksidan sıvılaştırılmış oksijen solüsyonu. URL: www.aktifanyonikoksijen.com (accessed date: December 24, 2021).
- Bursalı U, Kusbaygi H, Alkoclar EC. 2020. A biocidal active anionic H2O8 Oxygen solution with bronchodilative & oxygenizing features for the treatment of Coronaviral infection related hypoxia. J Design Sci, DOI: 10.5281/zenodo.4067635.
- Çolak A, Uçar Ö. 2007. Sütçü ineklerde vücut kondisyon skoru (VKS) ile fertilite ilişkisi. BÜLTENDİF Veteriner Bülten. 28 (Ağustos): 6-9.
- Dwyer CM. 2020. Can improving animal welfare contribute to sustainability and productivity? BSJ Agri, 3(1): 61-65.
- Gökçen H. 2008. İneklerde infertilite. Nobel Tıp Kitabevleri, İstanbul, Türkiye, pp: 3.
- Önder H. 2018. Nonparametric statistical methods used in biological experiments. BSJ Eng Sci, 1(1): 1-6.
- İleri, İ.K. 2002. Sun’i tohumlama teknikleri. Evcil Hayvanlarda Reprodüksiyon ve Sun’i Tohumlama. Ed. İleri, İ.K., Ak, K., Pabuccuoğlu S., Birler S. İstanbul Üniversitesi Veteriner Fakültesi Yayını. Ders Notu:133. İstanbul, Türkiye, pp: 144.
- Uçar Ö, Ağırbaş D. 2022. A holistic approach to the effects of intrauterine antimicrobial therapy on pregnancy rate after artificial insemination in dairy cows. BSJ Health Sci, 5(3): 591-596.
- Uçar Ö. 2021. Update on factors affecting the success of intrauterine insemination in ruminants. SMSLF 2021; 5th International Conference on Sustainable Management of Smart Livestock’s Farming 2021 (organised by University of Alexandria, EYPT. Virtual-Invited Presentation, November 3-5, 2021, Alexandria, Egypt.
- Uçar Ö, Bülbül T, Saatci M. 2020. The effects of insufficient management and nutrition on reproductive performance in dairy cows: Struggle between modern and traditional breeding. IV. International Congress on Domestic Animal Breeding, Genetics and Husbandry-ICABGEH-20, Abstract No: ICABGEH-2020-012. August 12-14, 2020, İzmir, Türkiye. pp: 103.
- Uçar Ö. 2006. Kafkas Üniversitesi Veteriner Fakültesi Dölerme ve Suni Tohumlama Anabilim Dalına getirilen hayvanların bireysel özellikleri, geliş zamanları ve uygulama yaklaşımları yönünden değerlendirilmesi. Atatürk Üniv Vet Bil Derg, 1(3- 4): 39-50.
Investigation of the Effect of Disinfectant and Antioxidant Intrauterine "Anionic Oxygen" Application on Fertility after Artificial Insemination in Dairy Cows
Öz
In the study, a total of 30 clinically healthy fertile Holstein cows (aged 3-8 years old; with 500-700 kg average live weight and 28 litres average daily milk) were used in a private dairy farm in Milas district of Muğla province. Randomly selected cows were divided into two groups (control and treatment) (n=15 cows/group) with almost similar body condition scores (BCS) (as all having approximately BCS of 2.75±0.25 units, 1-5 scale). Cows were monitored for natural oestrus, especially during the 2 months postpartum. Control cows (n=15) showing oestrus were inseminated (throughout the 3 consecutive occasions at maximum) by routine AI via recto-vaginal intrauterine route using frozen bull semen (0.25 ml dose; 7.5 x 106 total motile sperm) and thawing at 37 °C for 30 seconds. Inseminations were carried out for 7 months (from December 2021 to June 2022). For the treatment group, the cows were given the antimicrobial agent (UB20 O8®) intrauterine 30 minutes after each AI. The disinfectant dose, given to the corpus uteri region after the AI, was used as a total of 50 ml mixture (40 ml warm saline and 10 ml disinfectant). Throughout the study, animals were routinely given a farm-specific "dairy cow" diet (with 23% protein/concentrated feed). In addition, cows were provided regularly with clean drinking water, shade, and free movement in paddocks. Total pregnancy rates of cows and the 'pregnancy index' (the number of inseminations per pregnancy) of 35-60-day post-insemination as checked via ultrasound examination were analysed statistically by using Chi-Square and Wilcoxon tests. The possible difference between the groups based on pregnancy rate and pregnancy index data was considered significant at the P<0.05 level. According to the results obtained, numerical differences observed due to local treatment after all insemination periods were not statistically significant (P>0.05). Pregnancy rates following the 1st, 2nd, and 3rd inseminations in the treatment and control groups were 60.00% vs. 46.66%, 86.66% vs. 66.66%, and 93.33% vs. 80.00%, respectively. The total pregnancy indexes in the groups were 1.57 (14 pregnancies/22 inseminations; treatment) and 2.25 (12 pregnancies/27 inseminations; control). Briefly, it was observed that the pregnancy rate and pregnancy index in the treatment group were consistently but only numerically higher than those in the control group, indicating that anionic oxygen could be an effective alternative for intrauterine treatment leading to high fertility early postpartum.
Anahtar Kelimeler
Proje Numarası
2209 A, Proje No 1919B012109561 (2021/2. Dönem)
Kaynakça
- Apaydın Yıldırım B. 2021. Anti-cancer, antiproliferative activity of active anionic H208 oxygen solution on HCT-116 cancer cells. World J Adv Res Rev, 12(02): 179-184.
- BAYNOVA Catalogue. 2021. Ultra Biyosit enzimli aktif anyonik oktaoksidan sıvılaştırılmış oksijen solüsyonu. URL: www.aktifanyonikoksijen.com (accessed date: December 24, 2021).
- Bursalı U, Kusbaygi H, Alkoclar EC. 2020. A biocidal active anionic H2O8 Oxygen solution with bronchodilative & oxygenizing features for the treatment of Coronaviral infection related hypoxia. J Design Sci, DOI: 10.5281/zenodo.4067635.
- Çolak A, Uçar Ö. 2007. Sütçü ineklerde vücut kondisyon skoru (VKS) ile fertilite ilişkisi. BÜLTENDİF Veteriner Bülten. 28 (Ağustos): 6-9.
- Dwyer CM. 2020. Can improving animal welfare contribute to sustainability and productivity? BSJ Agri, 3(1): 61-65.
- Gökçen H. 2008. İneklerde infertilite. Nobel Tıp Kitabevleri, İstanbul, Türkiye, pp: 3.
- Önder H. 2018. Nonparametric statistical methods used in biological experiments. BSJ Eng Sci, 1(1): 1-6.
- İleri, İ.K. 2002. Sun’i tohumlama teknikleri. Evcil Hayvanlarda Reprodüksiyon ve Sun’i Tohumlama. Ed. İleri, İ.K., Ak, K., Pabuccuoğlu S., Birler S. İstanbul Üniversitesi Veteriner Fakültesi Yayını. Ders Notu:133. İstanbul, Türkiye, pp: 144.
- Uçar Ö, Ağırbaş D. 2022. A holistic approach to the effects of intrauterine antimicrobial therapy on pregnancy rate after artificial insemination in dairy cows. BSJ Health Sci, 5(3): 591-596.
- Uçar Ö. 2021. Update on factors affecting the success of intrauterine insemination in ruminants. SMSLF 2021; 5th International Conference on Sustainable Management of Smart Livestock’s Farming 2021 (organised by University of Alexandria, EYPT. Virtual-Invited Presentation, November 3-5, 2021, Alexandria, Egypt.
- Uçar Ö, Bülbül T, Saatci M. 2020. The effects of insufficient management and nutrition on reproductive performance in dairy cows: Struggle between modern and traditional breeding. IV. International Congress on Domestic Animal Breeding, Genetics and Husbandry-ICABGEH-20, Abstract No: ICABGEH-2020-012. August 12-14, 2020, İzmir, Türkiye. pp: 103.
- Uçar Ö. 2006. Kafkas Üniversitesi Veteriner Fakültesi Dölerme ve Suni Tohumlama Anabilim Dalına getirilen hayvanların bireysel özellikleri, geliş zamanları ve uygulama yaklaşımları yönünden değerlendirilmesi. Atatürk Üniv Vet Bil Derg, 1(3- 4): 39-50.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri (Diğer) |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | 2209 A, Proje No 1919B012109561 (2021/2. Dönem) |
| Erken Görünüm Tarihi | 11 Eylül 2023 |
| Yayımlanma Tarihi | 15 Ekim 2023 |
| Gönderilme Tarihi | 24 Temmuz 2023 |
| Kabul Tarihi | 4 Eylül 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 6 Sayı: 4 |
