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Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal

Year 2013, Volume: 1 Issue: 2, 76 - 80, 19.12.2014

Recai Zan Ihsan Hubbezoglu Ali Ozdemır Tutku Tunc Zeynep Sumer Oguzhan Alıcı

Abstract

The aim of this study was to investigate the antibacterial effects of
different concentration of boric acid in root canals that were
infected with Enterococcus faecalis biofilms. 104 single-canal
premolar teeth were prepared and then disinfected and sterilized.
E.faecalis were inoculated into the root canals and kept at 37°C for
24 hours. The re-inoculation procedure was repeated on the first,
fourth, seventh and tenth days with fresh culture. The infected root
canals were divided into one negative (saline), one positive
(sodium hypochlorite) control group, and three experimental
groups (boric acid; 2%, 4% and 6%) (n=20). Paper points were
placed in root canals before and after irrigation to control and
evaluate the biofilm formation. Biofilms were counted on blood
agar plates, and the data were evaluated and statistically analyzed
using one-way ANOVA and Tukey’s test. There were statistically
significant differences among three experimental group (boric
acid; 2%, 4% and 6%). Although 6% concentration of boric acid
indicated the highest antibacterial effect, it could not exhibit the
same antibacterial effect as NaOCl. As a new product boric acid
indicated considerable bactericidal effect against E.faecalis
biofilms. However, the use of boric acid can be advisable with high
concentrations and long irrigation time for root canals disinfection.

Keywords

Boric acid root canal microorganisms

References

  • Peters LB, Wesselink PR, Moorer WR. Penetration of bacteria in bovine root dentine in vitro. Int Endod J 2000;33:28-36.
  • Siren EK, Haapasalo MP, Ranta K, Salmi P, Kerosuo EN. Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J 1997;30:91-5.
  • Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1998;31:1-7.
  • Huth KC, Quirling M, Maier S, Kamereck K, Alkhayer M, Paschos E, Welsch U, Miethke T, Brand K, Hickel R. Effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm model. Int Endod J 2009;42:3-13.
  • Estrela C, Sydney GB, Figueiredo JA, Estrela CR. Antibacterial efficacy of intracanal medicaments on bacterial biofilm: a critical review. J Appl Oral Sci 2009;17:1-7.
  • Case PD, Bird PS, Kahler WA, George R, Walsh LJ. Treatment of root canal biofilms of Enterococcus faecalis with ozone gas and passive ultrasound activation. J Endod 2012;38:523-6.
  • Donlan RM. Biofilms and device-associated infections. Emerg Inf Dis 2001;7:277-81.
  • Jett BD, Huycke MM, Gilmore MS. Virulence of enterococci. Clin Microbiol Rev 1994;7:462–78.
  • Elsner HA, Sobottka I, Mack D. Virulence factors of Enterococcus faecalis and Enterococcus faecium blood culture isolates. Europ J Clin Microbiol Inf Dis 2000;19:39-42.
  • Ten Cate JM. Biofilms, a new approach to the microbiology of dental plaque. Odontol 2006;94:1-9.
  • Chávez de Paz L. Redefining the Persistent Infection in Root Canals: Possible Role of Biofilm Communities. J Endod 2007;33:652–62.
  • Niazi S, Clark D, Do T, Gilbert S, Foschi F, Mannocci F, Beighton D. The effectiveness of enzymic irrigation in removing a nutrient-stressed endodontic multi-species biofilm. Int Endod J 2013;Nov 18 [Epub ahead of print].
  • Alves FR, Neves MA, Silva MG, Rôças IN, Siqueira JF Jr. Antibiofilm and antibacterial activities of farnesol and xylitol as potential endodontic irrigants. Braz Dent J 2013;24:224-9.
  • Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
  • Becking AG. Complications in the use of sodium hypochlorite during endodontic treatment. Report of three cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1991;71:346-8.
  • Quarles W. Boric Acid, Borates and Household Pests. The IPM Practioner. Bio Integral Research Center, Berkeley, CA. 2001;23(3):1-12.
  • Saglam M, Arslan U, Buket Bozkurt S, Hakki SS. Boric acid irrigation as an adjunct to mechanical periodontal therapy in patients with chronic periodontitis: a randomized clinical trial. J Periodontol 2013;84:1297-308.
  • Demirer S, Kara MI, Erciyas K, Ozdemir H, Ozer H, Ay S. Effects of boric acid on experimental periodontitis and alveolar bone loss in rats. Arch Oral Biol 2012;57:60-65. Epub 2011 Aug 25.
  • Quarles W. Borates Provide Least-Toxic Wood Protection. The IPM Practioner. Bio-Integral Research Center, Berkeley, CA. 1992;14:1-11.
  • Saglam M, Hatipoglu M, Koseoglu S, Esen HH, Kelebek S. Boric acid inhibits alveolar bone loss in rats by affecting RANKL and osteoprotegerin expression. J Periodontal Res 2013 Aug 29 doi: 10.1111/jre.12126. [Epub ahead of print].
  • Prigent-Combaret, C, Vidal O, Dorel C, Lejeune P. Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli. J Bacteriol 1999;181:5993– 6002.
  • Parsek MR, Greenberg EP. Sociomicrobiology: the connections between quorum sensing and biofilms. Trends in Microbiol 2005;13:27–33.
  • Xu, KD, Stewart PS, Xia F, Huang CT, and McFeters GA. Spatial physiological heterogeneity in Pseudomonas aeruginosa biofilm is determined by oxygen availability. Appl Environ Microbiol 1998;64:4035-9.
  • Whiteley M, Bangera MG, Bumgarner RE, Parsek MR, Teitzel GM, Lory S, Greenberg EP. Gene expression in Pseudomonas aeruginosa biofilms. Nature 2001;413:860–4.
  • Waite RD, Papakonstantinopoulou A, Littler E, Curtis MA. Transcriptome analysis of Pseudomonas aeruginosa growth: comparison of gene expression in planktonic cultures and developing and mature biofilms. J Bacteriol 2005;187:6571–6.
  • Mikkelsen H, Duck Z, Lilley KS, Welch M. Interrelationships between colonies, biofilms, and planktonic cells of Pseudomonas aeruginosa. J Bacteriol 2007;189:2411-6.
  • Subbiya A, Mahalakshmi K, Pushpangadan S, Padmavathy K, Vivekanandan P, Sukumaran VG. Antibacterial efficacy of Mangifera indica L. kernel and Ocimum sanctum L. leaves against Enterococcus faecalis dentinal biofilm. J Conserv Dent. 2013;16,454-7.
  • Yazıcı S, Akşit H, Korkut O, Sunay B, Çelik T. Effects of boric acid and 2-aminoethoxydiphenyl borate on necrotizing enterocolitis. J Pediatr Gastroenterol Nutr 2014;58:61-7.
  • Ince S, Keles H, Erdogan M, Hazman O, Kucukkurt I. Protective effect of boric acid against carbon tetrachloride- induced hepatotoxicity in mice. Drug Chem Toxicol 2012;35:285-92.
  • Reichman O, Akins R, Sobel JD. Boric acid addition to suppressive antimicrobial therapy for recurrent bacterial vaginosis. Sex Transm Dis 2009;36:732-4.
  • Saita K, Nagaoka S, Shirosaki T, Horikawa M, Matsuda S, Ihara H. Preparation and characterization of dispersible chitosan particles with borate crosslinking and their antimicrobial and antifungal activity. Carbohydr Res 2012;349:52-8.

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Year 2013, Volume: 1 Issue: 2, 76 - 80, 19.12.2014

Recai Zan Ihsan Hubbezoglu Ali Ozdemır Tutku Tunc Zeynep Sumer Oguzhan Alıcı

Abstract

-

Keywords

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References

  • Peters LB, Wesselink PR, Moorer WR. Penetration of bacteria in bovine root dentine in vitro. Int Endod J 2000;33:28-36.
  • Siren EK, Haapasalo MP, Ranta K, Salmi P, Kerosuo EN. Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J 1997;30:91-5.
  • Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1998;31:1-7.
  • Huth KC, Quirling M, Maier S, Kamereck K, Alkhayer M, Paschos E, Welsch U, Miethke T, Brand K, Hickel R. Effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm model. Int Endod J 2009;42:3-13.
  • Estrela C, Sydney GB, Figueiredo JA, Estrela CR. Antibacterial efficacy of intracanal medicaments on bacterial biofilm: a critical review. J Appl Oral Sci 2009;17:1-7.
  • Case PD, Bird PS, Kahler WA, George R, Walsh LJ. Treatment of root canal biofilms of Enterococcus faecalis with ozone gas and passive ultrasound activation. J Endod 2012;38:523-6.
  • Donlan RM. Biofilms and device-associated infections. Emerg Inf Dis 2001;7:277-81.
  • Jett BD, Huycke MM, Gilmore MS. Virulence of enterococci. Clin Microbiol Rev 1994;7:462–78.
  • Elsner HA, Sobottka I, Mack D. Virulence factors of Enterococcus faecalis and Enterococcus faecium blood culture isolates. Europ J Clin Microbiol Inf Dis 2000;19:39-42.
  • Ten Cate JM. Biofilms, a new approach to the microbiology of dental plaque. Odontol 2006;94:1-9.
  • Chávez de Paz L. Redefining the Persistent Infection in Root Canals: Possible Role of Biofilm Communities. J Endod 2007;33:652–62.
  • Niazi S, Clark D, Do T, Gilbert S, Foschi F, Mannocci F, Beighton D. The effectiveness of enzymic irrigation in removing a nutrient-stressed endodontic multi-species biofilm. Int Endod J 2013;Nov 18 [Epub ahead of print].
  • Alves FR, Neves MA, Silva MG, Rôças IN, Siqueira JF Jr. Antibiofilm and antibacterial activities of farnesol and xylitol as potential endodontic irrigants. Braz Dent J 2013;24:224-9.
  • Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
  • Becking AG. Complications in the use of sodium hypochlorite during endodontic treatment. Report of three cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1991;71:346-8.
  • Quarles W. Boric Acid, Borates and Household Pests. The IPM Practioner. Bio Integral Research Center, Berkeley, CA. 2001;23(3):1-12.
  • Saglam M, Arslan U, Buket Bozkurt S, Hakki SS. Boric acid irrigation as an adjunct to mechanical periodontal therapy in patients with chronic periodontitis: a randomized clinical trial. J Periodontol 2013;84:1297-308.
  • Demirer S, Kara MI, Erciyas K, Ozdemir H, Ozer H, Ay S. Effects of boric acid on experimental periodontitis and alveolar bone loss in rats. Arch Oral Biol 2012;57:60-65. Epub 2011 Aug 25.
  • Quarles W. Borates Provide Least-Toxic Wood Protection. The IPM Practioner. Bio-Integral Research Center, Berkeley, CA. 1992;14:1-11.
  • Saglam M, Hatipoglu M, Koseoglu S, Esen HH, Kelebek S. Boric acid inhibits alveolar bone loss in rats by affecting RANKL and osteoprotegerin expression. J Periodontal Res 2013 Aug 29 doi: 10.1111/jre.12126. [Epub ahead of print].
  • Prigent-Combaret, C, Vidal O, Dorel C, Lejeune P. Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli. J Bacteriol 1999;181:5993– 6002.
  • Parsek MR, Greenberg EP. Sociomicrobiology: the connections between quorum sensing and biofilms. Trends in Microbiol 2005;13:27–33.
  • Xu, KD, Stewart PS, Xia F, Huang CT, and McFeters GA. Spatial physiological heterogeneity in Pseudomonas aeruginosa biofilm is determined by oxygen availability. Appl Environ Microbiol 1998;64:4035-9.
  • Whiteley M, Bangera MG, Bumgarner RE, Parsek MR, Teitzel GM, Lory S, Greenberg EP. Gene expression in Pseudomonas aeruginosa biofilms. Nature 2001;413:860–4.
  • Waite RD, Papakonstantinopoulou A, Littler E, Curtis MA. Transcriptome analysis of Pseudomonas aeruginosa growth: comparison of gene expression in planktonic cultures and developing and mature biofilms. J Bacteriol 2005;187:6571–6.
  • Mikkelsen H, Duck Z, Lilley KS, Welch M. Interrelationships between colonies, biofilms, and planktonic cells of Pseudomonas aeruginosa. J Bacteriol 2007;189:2411-6.
  • Subbiya A, Mahalakshmi K, Pushpangadan S, Padmavathy K, Vivekanandan P, Sukumaran VG. Antibacterial efficacy of Mangifera indica L. kernel and Ocimum sanctum L. leaves against Enterococcus faecalis dentinal biofilm. J Conserv Dent. 2013;16,454-7.
  • Yazıcı S, Akşit H, Korkut O, Sunay B, Çelik T. Effects of boric acid and 2-aminoethoxydiphenyl borate on necrotizing enterocolitis. J Pediatr Gastroenterol Nutr 2014;58:61-7.
  • Ince S, Keles H, Erdogan M, Hazman O, Kucukkurt I. Protective effect of boric acid against carbon tetrachloride- induced hepatotoxicity in mice. Drug Chem Toxicol 2012;35:285-92.
  • Reichman O, Akins R, Sobel JD. Boric acid addition to suppressive antimicrobial therapy for recurrent bacterial vaginosis. Sex Transm Dis 2009;36:732-4.
  • Saita K, Nagaoka S, Shirosaki T, Horikawa M, Matsuda S, Ihara H. Preparation and characterization of dispersible chitosan particles with borate crosslinking and their antimicrobial and antifungal activity. Carbohydr Res 2012;349:52-8.
There are 31 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Recai Zan

Ihsan Hubbezoglu This is me

Ali Ozdemır This is me

Tutku Tunc This is me

Zeynep Sumer This is me

Oguzhan Alıcı This is me

Publication Date December 19, 2014
Published in Issue Year 2013 Volume: 1 Issue: 2

Cite

APA Zan, R., Hubbezoglu, I., Ozdemır, A., Tunc, T., et al. (2014). Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal. Marmara Dental Journal, 1(2), 76-80. https://doi.org/10.12990/ MDJ.201317716
AMA Zan R, Hubbezoglu I, Ozdemır A, Tunc T, Sumer Z, Alıcı O. Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal. Marmara Dental Journal. December 2014;1(2):76-80. doi:10.12990/ MDJ.201317716
Chicago Zan, Recai, Ihsan Hubbezoglu, Ali Ozdemır, Tutku Tunc, Zeynep Sumer, and Oguzhan Alıcı. “Antibacterial Effect of Different Concentration of Boric Acid Against Enterococcus Faecalis Biofilms in Root Canal”. Marmara Dental Journal 1, no. 2 (December 2014): 76-80. https://doi.org/10.12990/ MDJ.201317716.
EndNote Zan R, Hubbezoglu I, Ozdemır A, Tunc T, Sumer Z, Alıcı O (December 1, 2014) Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal. Marmara Dental Journal 1 2 76–80.
IEEE R. Zan, I. Hubbezoglu, A. Ozdemır, T. Tunc, Z. Sumer, and O. Alıcı, “Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal”, Marmara Dental Journal, vol. 1, no. 2, pp. 76–80, 2014, doi: 10.12990/ MDJ.201317716.
ISNAD Zan, Recai et al. “Antibacterial Effect of Different Concentration of Boric Acid Against Enterococcus Faecalis Biofilms in Root Canal”. Marmara Dental Journal 1/2 (December 2014), 76-80. https://doi.org/10.12990/ MDJ.201317716.
JAMA Zan R, Hubbezoglu I, Ozdemır A, Tunc T, Sumer Z, Alıcı O. Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal. Marmara Dental Journal. 2014;1:76–80.
MLA Zan, Recai et al. “Antibacterial Effect of Different Concentration of Boric Acid Against Enterococcus Faecalis Biofilms in Root Canal”. Marmara Dental Journal, vol. 1, no. 2, 2014, pp. 76-80, doi:10.12990/ MDJ.201317716.
Vancouver Zan R, Hubbezoglu I, Ozdemır A, Tunc T, Sumer Z, Alıcı O. Antibacterial Effect of Different Concentration of Boric acid against Enterococcus Faecalis Biofilms in Root Canal. Marmara Dental Journal. 2014;1(2):76-80.