Research Article
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Year 2020, Volume: 23 Issue: 3, 191 - 199, 05.10.2020

Cansu Koçyiğit , Burcu Nihan Yüksel , Nurhan Özalp

https://doi.org/10.7126/cumudj.738837
Cited By: 1

Abstract

Supporting Institution

Ankara Üniversitesi Bilimsel Araştırma Projeleri Müdürlüğü

Project Number

14L0234001

References

  • Toumba KJ, Twetman S, Splieth C, Parnell C, van Loveren C, Lygidakis NΑ Guidelines on the use of fluoride for caries prevention in children: an updated EAPD policy document. Eur Arch Paediatr Dent. 2019;20:507-16.
  • Koruyucu M, Birant S, Topcuoglu S, Külekci G, Seymen F. Investigation of the Clinical and Microbiological Effects of Different Toothpastes: In-Vivo Study. Cumhuriyet Dent J 2020;23:22-31.
  • American Academy of Pediatric Dentistry (AAPD). Fluoride therapy 2018;40:250-3.
  • Cochrane NJ, Cai F, Huq NL, Burrow MF, Reynolds EC. New approaches to enhanced remineralization of tooth enamel. J Dent Res 2010;89:1187-97.
  • American Academy of Pediatric Dentistry (AAPD). Policy on Early Childhood Caries (ECC): Classifications, Consequences, and Preventive Strategies. Pediatr Dent 2016;38:52-4
  • Roveri N, Battistella E, Bianchi CL, Foltran I, Foresti E, Iafisco M, et al. Surface enamel remineralization: Biomimetic apatite nanocrystals and fluoride ions different effects. J Nanomater 2009;Article ID:746383:1-9.
  • Roveri N, Foresti E, Lelli M, Lesci IG. Recent advances in preventing teeth health hazard: The daily use of hydroxyapatite instead of fluoride. Recent Pat Biomed Eng 2009;2:197-215.
  • Swarup JS, Rao A. Enamel surface remineralization: Using synthetic nano-hydroxyapatite. Contemp Clin Dent 2012;3:433-6.
  • Huang S, Gao S, Cheng L, Yu H. Remineralization potential of nano-hydroxyapatite on initial enamel lesions: An in vitro study. Caries Res 2011;45:460-8.
  • Nozari A, Ajami S, Rafiei A, Niazi E. Impact of Nano hydroxyapatite, Nano silver fluoride and sodium fluoride varnish on primary teeth enamel remineralization: an in vitro study. J Clin Diagn Res 2017;11:ZC97-ZC100.
  • Haghgoo R, Ahmadvand M, Moshaverinia S. Remineralizing effect of topical NovaMin and Nano-hydroxyapatite on caries-like lesions in primary teeth. J Contemp Dent Pract 2016;17:645-9.
  • Kamath P, Nayak R, Kamath SU, Pai D. A comparative evaluation of the remineralization potential of three commercially available remineralizing agents on white spot lesions in primary teeth: An in vitro study. J Indian Soc Pedod Prev Dent 2017;35:229-37.
  • Grewal N, Sharma N, Kaur, N. Surface remineralization potential of nano-hydroxyapatite, sodium monofluorophosphate, and amine fluoride containing dentifrices on primary and permanent enamel surfaces: An in vitro study. J Indian Soc Pedod Prev Dent 2018;36:158-66.
  • Pepla E, Besharat LK, Palaia G, Tenore G, Migliau G. Nano-hydroxyapatite and its applications in preventive, restorative and regenerative dentistry: A review of literature. Ann Stomatol (Roma) 2014;5:108-14.
  • Huang SB, Gao S, Yu H. Effect of nano-hydroxyapatite concentration on remineralization of initial enamel lesion in vitro. Biomed Mater 2009;4:034104.
  • Itthagarun A, Cheung YM, King NM. The effect of nano-hydroxyapatite containing dentifrice on artificial enamel carious lesion progression: An in vitro pH cycling study. Hong Kong Dent J 2010;7:61-6.
  • Enax J, Epple M. Synthetic Hydroxyapatite as a Biomimetic Oral Care Agent. Oral Health Prev Dent 2018;16:7-19.
  • Jeong SH, Jang SO, Kim KN, Kwon HK, Park YD, Kim BI. Remineralisation potential of new dentifrice containing nano-hydroxyapatite. Key Eng Mater 2006;309:537-40.
  • Hornby K, Evans M Long, M, Bebington AJ. Enamel benefits of a new hydroxyapatite containing fluoride dentifrice. Int Dent J 2009;59:325-31.
  • Mielczarek A, Michalik J. The effect of nano-hydroxyapatite dentifrice on enamel surface remineralization. An in vitro study. Am J Dent 2014;27:287-90.
  • Mielczarek A, Gedrange T, Michalik J. An in vitro evaluation of the effect of fluoride products on white spot lesion remineralization. Am J Dent 2015;28:51-6.
  • Nakashima S, Yoshie M, Sano H, Bahar A. Effect of a test dentifrice containing nano-sized calcium carbonate on remineralization of enamel lesions in vitro. J Oral Sci 2009;51:69-77.
  • Huang S, Gao S, Cheng L, Yu H. Combined effects of nano-hydroxyapatite and Galla chinensis on remineralisation of initial enamel lesion in vitro. J Dent 2010;38:811-9.
  • Dağ C, Özalp N. Ağız-diş sağlığının vazgeçilmezi: Diş macunları. Acta Odontol Turc 2013;30:149-56.
  • Hannig M, Hannig C. Nanotechnology and its role in caries therapy. Adv Dent Res 2012;24:53-7.
  • Kani T, Kani M, Isozaki A, Shintani H, Ohashi T, Tokumoto T. Effect of apatite-containing dentifrices on dental caries in school children. J Dent Health 1989;39:104-9.
  • Hellwig E, Altenburger M, Attin T, Lussi A, Buchalla W. Remineralization of initial carious lesions in deciduous enamel after application of dentifrices of different fluoride concentrations. Clin Oral Investig 2010;14:265-9.
  • Ammari AB, Bloch-Zupan A, Ashley PF Systematic review of studies comparing the anti-caries efficacy of children’s dentifrice containing 600 ppm of fluoride or less with high fluoride dentifrices of 1,000 ppm or above. Caries Res 2003;37:85-92.
  • Lelli, M, Putignano A, Marchetti M, Foltran I, Mangani F, Procaccini, M, et al. Remineralization and repair of enamel surface by biomimetic Zn-carbonate hydroxyapatite containing dentifrice: A comparative in vivo study. Front Physiol 2014;5:1-6.
  • Tschoppe P, Zandim DL, Martur P, Kielbassa AM. Enamel and dentine remineralization by nano-hydroxyapatite dentifrices. J Dent 2011;39:430-7.
  • El Assal DW, Saafan AM, Moustafa DH, Al-Sayed MA. The effect of combining laser and nanohydroxy-apatite on the surface properties of enamel with initial defects. J Clin Exp Dent 2018;10:e425-e430.
  • Souza BM, Comar LP, Vertuan M, Neto CF, Buzalaf MAR, Magalhães AC. Effect of an experimental paste with hydroxyapatite nanoparticles and fluoride on dental demineralisation and remineralisation in situ. Caries Res 2015;49:499-507.
  • de Carvalho FG, Vieira BR, Santos RLD, Carlo HL, Lopes PQ, de Lima BASG. In vitro effects of nano-hydroxyapatite paste on initial enamel carious lesions. Pediatr Dent 2014;36:85-9.
  • Amaechi BT, van Loveren C. Fluorides and Non-Fluoride Remineralization Systems. In van Loveren C(ed). Toothpastes. Basel, Karger, 2013.Vol:23 pp:15-26.

EFFECTS OF NANO-HYDROXYAPATITE DENTIFRICES WITH AND WITHOUT FLUORIDE ON PRIMARY TEETH ENAMEL: A MICRO-CT AND A SEM STUDY

Year 2020, Volume: 23 Issue: 3, 191 - 199, 05.10.2020

Cansu Koçyiğit , Burcu Nihan Yüksel , Nurhan Özalp

https://doi.org/10.7126/cumudj.738837
Cited By: 1

Abstract

Objectives: The ideal remineralization system should provide sufficient ion reserves to provide mineral gain on deeper surfaces, as well as being biocompatible and easy to apply with no adverse effect. The aim of this study was to evaluate the remineralization efficiency of dentifrice form of nano-hydroxyapatite alone and its combination with fluoride on initial enamel lesions in primary teeth by micro-CT and SEM. Materials and Methods: The enamel surfaces of 30 extracted primary incisors were treated with nano-hydroxyapatite based (nHAP), nano-hydroxyapatite-fluoride-containing (nHAP+F) and fluoride-containing dentifrice (F), respectively after the pH cycle. Mineral gain and surface properties were evaluated by micro-CT and SEM.Results: nHAP group provided more mineral gain than fluoride containing paste groups but there was no statistically significant difference (p>0.05) according to the micro-CT evaluations.In SEM images, the micropores were completely covered with an apatite layer and mineral precipitations were observed to be wider and more pronounced in the nHAP group. Calcium fluoride crystalline structures exhibited non-homogeneous globular precipitations in the F Group.Conclusions: It was concluded that nHAP dentifrice is as effective as fluoride dentifrice on initial enamel lesions in primary teeth with less caries-resistant enamel surface and therefore it can be safely used.

Keywords

Dentifrices Hydroxyapatite Microcomputed Tomography Preventive Dentistry Tooth remineralization

Project Number

14L0234001

References

  • Toumba KJ, Twetman S, Splieth C, Parnell C, van Loveren C, Lygidakis NΑ Guidelines on the use of fluoride for caries prevention in children: an updated EAPD policy document. Eur Arch Paediatr Dent. 2019;20:507-16.
  • Koruyucu M, Birant S, Topcuoglu S, Külekci G, Seymen F. Investigation of the Clinical and Microbiological Effects of Different Toothpastes: In-Vivo Study. Cumhuriyet Dent J 2020;23:22-31.
  • American Academy of Pediatric Dentistry (AAPD). Fluoride therapy 2018;40:250-3.
  • Cochrane NJ, Cai F, Huq NL, Burrow MF, Reynolds EC. New approaches to enhanced remineralization of tooth enamel. J Dent Res 2010;89:1187-97.
  • American Academy of Pediatric Dentistry (AAPD). Policy on Early Childhood Caries (ECC): Classifications, Consequences, and Preventive Strategies. Pediatr Dent 2016;38:52-4
  • Roveri N, Battistella E, Bianchi CL, Foltran I, Foresti E, Iafisco M, et al. Surface enamel remineralization: Biomimetic apatite nanocrystals and fluoride ions different effects. J Nanomater 2009;Article ID:746383:1-9.
  • Roveri N, Foresti E, Lelli M, Lesci IG. Recent advances in preventing teeth health hazard: The daily use of hydroxyapatite instead of fluoride. Recent Pat Biomed Eng 2009;2:197-215.
  • Swarup JS, Rao A. Enamel surface remineralization: Using synthetic nano-hydroxyapatite. Contemp Clin Dent 2012;3:433-6.
  • Huang S, Gao S, Cheng L, Yu H. Remineralization potential of nano-hydroxyapatite on initial enamel lesions: An in vitro study. Caries Res 2011;45:460-8.
  • Nozari A, Ajami S, Rafiei A, Niazi E. Impact of Nano hydroxyapatite, Nano silver fluoride and sodium fluoride varnish on primary teeth enamel remineralization: an in vitro study. J Clin Diagn Res 2017;11:ZC97-ZC100.
  • Haghgoo R, Ahmadvand M, Moshaverinia S. Remineralizing effect of topical NovaMin and Nano-hydroxyapatite on caries-like lesions in primary teeth. J Contemp Dent Pract 2016;17:645-9.
  • Kamath P, Nayak R, Kamath SU, Pai D. A comparative evaluation of the remineralization potential of three commercially available remineralizing agents on white spot lesions in primary teeth: An in vitro study. J Indian Soc Pedod Prev Dent 2017;35:229-37.
  • Grewal N, Sharma N, Kaur, N. Surface remineralization potential of nano-hydroxyapatite, sodium monofluorophosphate, and amine fluoride containing dentifrices on primary and permanent enamel surfaces: An in vitro study. J Indian Soc Pedod Prev Dent 2018;36:158-66.
  • Pepla E, Besharat LK, Palaia G, Tenore G, Migliau G. Nano-hydroxyapatite and its applications in preventive, restorative and regenerative dentistry: A review of literature. Ann Stomatol (Roma) 2014;5:108-14.
  • Huang SB, Gao S, Yu H. Effect of nano-hydroxyapatite concentration on remineralization of initial enamel lesion in vitro. Biomed Mater 2009;4:034104.
  • Itthagarun A, Cheung YM, King NM. The effect of nano-hydroxyapatite containing dentifrice on artificial enamel carious lesion progression: An in vitro pH cycling study. Hong Kong Dent J 2010;7:61-6.
  • Enax J, Epple M. Synthetic Hydroxyapatite as a Biomimetic Oral Care Agent. Oral Health Prev Dent 2018;16:7-19.
  • Jeong SH, Jang SO, Kim KN, Kwon HK, Park YD, Kim BI. Remineralisation potential of new dentifrice containing nano-hydroxyapatite. Key Eng Mater 2006;309:537-40.
  • Hornby K, Evans M Long, M, Bebington AJ. Enamel benefits of a new hydroxyapatite containing fluoride dentifrice. Int Dent J 2009;59:325-31.
  • Mielczarek A, Michalik J. The effect of nano-hydroxyapatite dentifrice on enamel surface remineralization. An in vitro study. Am J Dent 2014;27:287-90.
  • Mielczarek A, Gedrange T, Michalik J. An in vitro evaluation of the effect of fluoride products on white spot lesion remineralization. Am J Dent 2015;28:51-6.
  • Nakashima S, Yoshie M, Sano H, Bahar A. Effect of a test dentifrice containing nano-sized calcium carbonate on remineralization of enamel lesions in vitro. J Oral Sci 2009;51:69-77.
  • Huang S, Gao S, Cheng L, Yu H. Combined effects of nano-hydroxyapatite and Galla chinensis on remineralisation of initial enamel lesion in vitro. J Dent 2010;38:811-9.
  • Dağ C, Özalp N. Ağız-diş sağlığının vazgeçilmezi: Diş macunları. Acta Odontol Turc 2013;30:149-56.
  • Hannig M, Hannig C. Nanotechnology and its role in caries therapy. Adv Dent Res 2012;24:53-7.
  • Kani T, Kani M, Isozaki A, Shintani H, Ohashi T, Tokumoto T. Effect of apatite-containing dentifrices on dental caries in school children. J Dent Health 1989;39:104-9.
  • Hellwig E, Altenburger M, Attin T, Lussi A, Buchalla W. Remineralization of initial carious lesions in deciduous enamel after application of dentifrices of different fluoride concentrations. Clin Oral Investig 2010;14:265-9.
  • Ammari AB, Bloch-Zupan A, Ashley PF Systematic review of studies comparing the anti-caries efficacy of children’s dentifrice containing 600 ppm of fluoride or less with high fluoride dentifrices of 1,000 ppm or above. Caries Res 2003;37:85-92.
  • Lelli, M, Putignano A, Marchetti M, Foltran I, Mangani F, Procaccini, M, et al. Remineralization and repair of enamel surface by biomimetic Zn-carbonate hydroxyapatite containing dentifrice: A comparative in vivo study. Front Physiol 2014;5:1-6.
  • Tschoppe P, Zandim DL, Martur P, Kielbassa AM. Enamel and dentine remineralization by nano-hydroxyapatite dentifrices. J Dent 2011;39:430-7.
  • El Assal DW, Saafan AM, Moustafa DH, Al-Sayed MA. The effect of combining laser and nanohydroxy-apatite on the surface properties of enamel with initial defects. J Clin Exp Dent 2018;10:e425-e430.
  • Souza BM, Comar LP, Vertuan M, Neto CF, Buzalaf MAR, Magalhães AC. Effect of an experimental paste with hydroxyapatite nanoparticles and fluoride on dental demineralisation and remineralisation in situ. Caries Res 2015;49:499-507.
  • de Carvalho FG, Vieira BR, Santos RLD, Carlo HL, Lopes PQ, de Lima BASG. In vitro effects of nano-hydroxyapatite paste on initial enamel carious lesions. Pediatr Dent 2014;36:85-9.
  • Amaechi BT, van Loveren C. Fluorides and Non-Fluoride Remineralization Systems. In van Loveren C(ed). Toothpastes. Basel, Karger, 2013.Vol:23 pp:15-26.
There are 34 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research Articles
Authors

Cansu Koçyiğit 0000-0003-4475-1722

Burcu Nihan Yüksel 0000-0002-8133-6627

Nurhan Özalp 0000-0003-4192-2960

Project Number 14L0234001
Publication Date October 5, 2020
Submission Date May 17, 2020
Published in Issue Year 2020Volume: 23 Issue: 3

Cite

EndNote Koçyiğit C, Yüksel BN, Özalp N (October 1, 2020) EFFECTS OF NANO-HYDROXYAPATITE DENTIFRICES WITH AND WITHOUT FLUORIDE ON PRIMARY TEETH ENAMEL: A MICRO-CT AND A SEM STUDY. Cumhuriyet Dental Journal 23 3 191–199.

Cited By

Effects of Five Different Toothpastes on Remineralization and Surface Roughness of Primary Tooth Enamel with Artificial Initial Caries
Applied Sciences
https://doi.org/10.3390/app14167232
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