Research Article
Assessment of the Effectiveness of Ozone Theraphy and an Antibacterial Bonding Agent on the Cavity Disinfection of Deciduous Teeth: An In Vivo Study
Abstract
Objectives:The aim of this study is to evaluate
antibacterial effectiveness of 30 second ozone therapy applied via OzonyTronX,
Clearfil Protect Bond(a MDPB containing bonding agent) and Dycal( Ca(OH)2
containing cavity lining material) on S. mutans in deciduos teeth.
antibacterial effectiveness of 30 second ozone therapy applied via OzonyTronX,
Clearfil Protect Bond(a MDPB containing bonding agent) and Dycal( Ca(OH)2
containing cavity lining material) on S. mutans in deciduos teeth.
Materials and Methods:40 primary molars were obtained from
ten patients whose ages ranged between 5 and 11. Dentin samples which were
collected before the treatment and after a period of four weeks following the
implementation of materials were microbiologically evaluated and material’s
antibacterial effectiveness were compared.
ten patients whose ages ranged between 5 and 11. Dentin samples which were
collected before the treatment and after a period of four weeks following the
implementation of materials were microbiologically evaluated and material’s
antibacterial effectiveness were compared.
Results:Differences between the antibacterial
effectiveness of the materials were found to be statistically significant according to the results of covariance
analysis with randomized block design(p<0.05). Order of success in our
study, which evaluated antibacterial effectiveness of the groups on S. mutants,
is as follows: Group 2( ozone therapy)> Group 3(CPB)> Group 1(Dycal).
effectiveness of the materials were found to be statistically significant according to the results of covariance
analysis with randomized block design(p<0.05). Order of success in our
study, which evaluated antibacterial effectiveness of the groups on S. mutants,
is as follows: Group 2( ozone therapy)> Group 3(CPB)> Group 1(Dycal).
Conclusion: While CPB, which is an
antibacterial self etching system, and ozone therapy do not increase the
duration of clinical treatments, they can be efficient solutions for the
restorative treatment of primary teeth.
Supporting Institution
Ankara Üniversitesi BAP
Project Number
grant number 08B3334003
Thanks
The authors declare no conflict of interest.
References
- Bjørndal L, Larsen T, Thylstrup A. A clinical and microbiological study of deep carious lesions during stepwise excavation using long treatment intervals. Caries Res, 1997;31(6):411–417.
- Kidd EA. How 'clean' must a cavity be before restoration? Caries Res, 2004; 38: 305-13.
- Orhan AI, Oz FT, Ozcelik B, Ozgul BM. A clinical and microbiological comparative study of deep carious lesion treatment in deciduous and young permanent molars. Clin Oral Invest, 2008;12(4):369–378.
- Ricketts DN, Kidd EA, Beighton D. Operative and microbiological validation of visual, radiographic and electronic diagnosis of occlusal caries in non-cavitated teeth judged to be in need of operative care. Br Dent J, 1995;179 (6):214–220.
- Sancakli HS, Siso SH, Yildiz SO, Gökçe YB. Antibacterial effect of surface pretreatment techniques against Streptococcus mutans. Nigerian Journal of Clinical Practice, 2018;21(2):170-175.
- Weerheijm KL, Kreulen CM, de Soet JJ, et al. Bacterial counts in carious dentine under restorations: 2-year in vivo effects. Caries Res, 1999;33(2):130–134.
- Bjørndal L, Larsen T. Changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure. Caries Res, 2000;34(6):502–508.
- Dummett OC, KopeL MH. Pediatric Endodontics. In:Endodontics. Ed: Ingle JT, Bakland LK. 5th Ed.London;BC Decker Inc, Hamilton;2002. pp. 861-902.
- Deshpande P, Nainan MT, Metta KK, et al. The comparative evaluation of antibacterial activity of methacryloxydodecyl pyridinium bromide and non-methacryloxydodecyl pyridinium bromide dentin bonding systems using two different techniques: an in vitro study. J Int Oral Health, 2014;6(5):60–65.
- Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent. Mater, 2003; 19: 313-9.
- Schmalz G, Ergucu Z, Hiller KA. Effect of dentin on the antibacterial activity of dentin bonding agents. J. Endod, 2004;30: 352-8.
- Imazato, S., Kaneko, T., Takahashi, Y., Noiri, Y., Ebisu, S. In vivo antibacterial effects of dentin primer incorporating MDPB. Oper. Dent,2004; 29: 369-75.
- Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci, 2006;114(4):349–353.
- Polydorou O, Halili A, Wittmer A, Pelz K, Hahn P. The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clin. Oral. Invest, 2012; 16(2): 545-550.
- Turkun M, Turkun LS, Ateş M. Antibacterial activity of a self-etching adhesive system containing. “MDPD",GÜ Dişhek Fak Derg, 2003; 20, 41-6.
- Almaz ME, Sönmez IŞ . Ozone therapy in the management and prevention of caries. Journal of the formosan medical association, 2015;114(1):3-11
- Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone-generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res, 2000;34:498–501.
- Cangul S, Yıldırım ZS, Bahsi E, Sagmak S, Satıcı O. Do ozone and boric acid affect microleakage in class V composite restorations?The Journal of the Internationale Ozone Association, 2019;41(1): 92-101.
- Gokcen EY, Oz FT, Ozcelik B, Orhan AI, Ozgul BM. Assessment of antibacterial activity of different treatment modalities in deciduous teeth: an in vitro study Biotechnology & Biotechnological Equipment,2016;30(6):1192-1198.
- Neut D, Van De Belt, Stokroos I, van Horn JR., van Der Meı HC, Busscher HJ. Biomaterial-associated infection of gentamicin-loaded PMMA beads in orthopaedic revision surgery. J. Antimicrob. Chemother,2001; 47: 885-91.
- Kidd EA, Joyston-Bechal S, Beighton D. Microbiological validation of assessments of caries activity during cavity preparation. Caries Res, 1993;27(5):402–408.
- Lager A, Thornqvist E, Ericson D. Cultivatable bacteria in dentine after caries excavation using rose-bur or carisolv. Caries Res, 2003;37: 206-11.
- Wambier DS, Dos Santos FA, Guedes-Pinto AC, Jaeger RG, Simionato MR. Ultrastructural and microbiological analysis of the dentin layers affected by caries lesions in primary molars treated by minimal intervention. Pediatr. Dent, 2007; 29: 228-34.
- Pinto AS, de Araujo FB, Franzon R, et al. Clinical and microbiological effect of calcium hydroxide protection in indirect pulp capping in primary teeth. Am J Dent, 2006;19 (6):382–386.
- Azarpazhooh A, Limeback H. The application of ozone in dentistry: a systematic review of literature. J Dent, 2008;36:104-16.
- Nagayoshi M, Kitamura C, Fukuizumi T, et al. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod, 2004;30(11):778–781.
- Kidd EA, Beighton D. Prediction of secondary caries around tooth-colored restorations: a clinical and microbiological study. J. Dent. Res, 1996; 75: 1942-6.
- Moll K, Fritzenschaft A, Haller B. In vitro comparison of dentin bonding systems: effect of testing method and operator. Quintessence Int, 2004;35(10):845–852.
- Ozer F, Karakaya S, Unlu N, et al. Comparison of antibacterial activity of two dentin bonding systems using agar well technique and tooth cavity model. J Dent, 2003;31 (2):111–116.
- Hickel R, Dasch W, Janda R, Tyas M, Anusavıce K. New direct restorative materials.. Inter. Dent. J, 1998; 48: 3-16.
- Mclean JW, Wilson AD. Glass-ionomer cements. Br. Dent. J, 2004; 196: 514-5.
- Leung RL, Loesche WJ, Charbeneau GT. Effect of Dycal on bacteria in deep carious lesions. J. Am. Dent. Assoc,1980; 100: 193-7.
- Hauser-Gerspach I, Pfaffli-Savtchenko V, Dahnhardt JE, Meyer J, Lussi A. Comparison of the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children in vivo. Clin Oral Investig, 2009;13: 287-91.
- Atabek D, Oztas N. Effectiveness of ozone with or without the additional use of remineralizing solution on non-cavitated fissure carious. Eur J Dent.2011 Oct;5(4):393-9.
Year 2019,
Volume: 22 Issue: 4, 442 - 450, 29.12.2019
Abstract
Project Number
grant number 08B3334003
References
- Bjørndal L, Larsen T, Thylstrup A. A clinical and microbiological study of deep carious lesions during stepwise excavation using long treatment intervals. Caries Res, 1997;31(6):411–417.
- Kidd EA. How 'clean' must a cavity be before restoration? Caries Res, 2004; 38: 305-13.
- Orhan AI, Oz FT, Ozcelik B, Ozgul BM. A clinical and microbiological comparative study of deep carious lesion treatment in deciduous and young permanent molars. Clin Oral Invest, 2008;12(4):369–378.
- Ricketts DN, Kidd EA, Beighton D. Operative and microbiological validation of visual, radiographic and electronic diagnosis of occlusal caries in non-cavitated teeth judged to be in need of operative care. Br Dent J, 1995;179 (6):214–220.
- Sancakli HS, Siso SH, Yildiz SO, Gökçe YB. Antibacterial effect of surface pretreatment techniques against Streptococcus mutans. Nigerian Journal of Clinical Practice, 2018;21(2):170-175.
- Weerheijm KL, Kreulen CM, de Soet JJ, et al. Bacterial counts in carious dentine under restorations: 2-year in vivo effects. Caries Res, 1999;33(2):130–134.
- Bjørndal L, Larsen T. Changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure. Caries Res, 2000;34(6):502–508.
- Dummett OC, KopeL MH. Pediatric Endodontics. In:Endodontics. Ed: Ingle JT, Bakland LK. 5th Ed.London;BC Decker Inc, Hamilton;2002. pp. 861-902.
- Deshpande P, Nainan MT, Metta KK, et al. The comparative evaluation of antibacterial activity of methacryloxydodecyl pyridinium bromide and non-methacryloxydodecyl pyridinium bromide dentin bonding systems using two different techniques: an in vitro study. J Int Oral Health, 2014;6(5):60–65.
- Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent. Mater, 2003; 19: 313-9.
- Schmalz G, Ergucu Z, Hiller KA. Effect of dentin on the antibacterial activity of dentin bonding agents. J. Endod, 2004;30: 352-8.
- Imazato, S., Kaneko, T., Takahashi, Y., Noiri, Y., Ebisu, S. In vivo antibacterial effects of dentin primer incorporating MDPB. Oper. Dent,2004; 29: 369-75.
- Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci, 2006;114(4):349–353.
- Polydorou O, Halili A, Wittmer A, Pelz K, Hahn P. The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clin. Oral. Invest, 2012; 16(2): 545-550.
- Turkun M, Turkun LS, Ateş M. Antibacterial activity of a self-etching adhesive system containing. “MDPD",GÜ Dişhek Fak Derg, 2003; 20, 41-6.
- Almaz ME, Sönmez IŞ . Ozone therapy in the management and prevention of caries. Journal of the formosan medical association, 2015;114(1):3-11
- Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone-generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res, 2000;34:498–501.
- Cangul S, Yıldırım ZS, Bahsi E, Sagmak S, Satıcı O. Do ozone and boric acid affect microleakage in class V composite restorations?The Journal of the Internationale Ozone Association, 2019;41(1): 92-101.
- Gokcen EY, Oz FT, Ozcelik B, Orhan AI, Ozgul BM. Assessment of antibacterial activity of different treatment modalities in deciduous teeth: an in vitro study Biotechnology & Biotechnological Equipment,2016;30(6):1192-1198.
- Neut D, Van De Belt, Stokroos I, van Horn JR., van Der Meı HC, Busscher HJ. Biomaterial-associated infection of gentamicin-loaded PMMA beads in orthopaedic revision surgery. J. Antimicrob. Chemother,2001; 47: 885-91.
- Kidd EA, Joyston-Bechal S, Beighton D. Microbiological validation of assessments of caries activity during cavity preparation. Caries Res, 1993;27(5):402–408.
- Lager A, Thornqvist E, Ericson D. Cultivatable bacteria in dentine after caries excavation using rose-bur or carisolv. Caries Res, 2003;37: 206-11.
- Wambier DS, Dos Santos FA, Guedes-Pinto AC, Jaeger RG, Simionato MR. Ultrastructural and microbiological analysis of the dentin layers affected by caries lesions in primary molars treated by minimal intervention. Pediatr. Dent, 2007; 29: 228-34.
- Pinto AS, de Araujo FB, Franzon R, et al. Clinical and microbiological effect of calcium hydroxide protection in indirect pulp capping in primary teeth. Am J Dent, 2006;19 (6):382–386.
- Azarpazhooh A, Limeback H. The application of ozone in dentistry: a systematic review of literature. J Dent, 2008;36:104-16.
- Nagayoshi M, Kitamura C, Fukuizumi T, et al. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod, 2004;30(11):778–781.
- Kidd EA, Beighton D. Prediction of secondary caries around tooth-colored restorations: a clinical and microbiological study. J. Dent. Res, 1996; 75: 1942-6.
- Moll K, Fritzenschaft A, Haller B. In vitro comparison of dentin bonding systems: effect of testing method and operator. Quintessence Int, 2004;35(10):845–852.
- Ozer F, Karakaya S, Unlu N, et al. Comparison of antibacterial activity of two dentin bonding systems using agar well technique and tooth cavity model. J Dent, 2003;31 (2):111–116.
- Hickel R, Dasch W, Janda R, Tyas M, Anusavıce K. New direct restorative materials.. Inter. Dent. J, 1998; 48: 3-16.
- Mclean JW, Wilson AD. Glass-ionomer cements. Br. Dent. J, 2004; 196: 514-5.
- Leung RL, Loesche WJ, Charbeneau GT. Effect of Dycal on bacteria in deep carious lesions. J. Am. Dent. Assoc,1980; 100: 193-7.
- Hauser-Gerspach I, Pfaffli-Savtchenko V, Dahnhardt JE, Meyer J, Lussi A. Comparison of the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children in vivo. Clin Oral Investig, 2009;13: 287-91.
- Atabek D, Oztas N. Effectiveness of ozone with or without the additional use of remineralizing solution on non-cavitated fissure carious. Eur J Dent.2011 Oct;5(4):393-9.
There are 34 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Research Articles |
| Authors | |
| Project Number | grant number 08B3334003 |
| Publication Date | December 29, 2019 |
| Submission Date | November 5, 2019 |
| Published in Issue | Year 2019Volume: 22 Issue: 4 |
Cited By
Antimicrobial Effect of Ozone Therapy in Deep Dentinal Carious Lesion: A Systematic Review
International Journal of Clinical Pediatric Dentistry
https://doi.org/10.5005/jp-journals-10005-2168
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Murat ÇETİNKAYA
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